Upload
chad-carr
View
245
Download
0
Tags:
Embed Size (px)
Citation preview
Biological Membranes
Lipid Bilayer
• Phospholipids form bilayers in water
• Fluid mosaic model of membrane structure
• Membrane is only 10 nm thick
• Proteins move around laterally like icebergs in the ocean
Hydrophilic
Hydrophobic
AmphipathicAmphipathicAmphipathicAmphipathic
Bilayer
• Phospholipids form bilayers because the molecules….– Have two distinct regions, one
strongly hydrophobic and the other strongly hydrophilic
– Have cylindrical shapes that allow them to associate with water most easily as a bilayer structure
Fluid Mosaic Model
• Singer and Nicholson in 1972
• Phospholipid bilayer with proteins embedded within the membrane or otherwise associated with the membrane
• Dynamic layer- molecules move about laterally in the membrane
Membranes are two-dimensional fluids
Membranes are two-dimensional fluids
Molecules move laterallyPhospholipids can rotateBut stay within their layer
Frye and Edidin (1970)Fused and labeled human and mouse cells and noted that the some of the molecules in the cell membrane had indeed moved
Michael Edidin
Graduate Professor at Johns Hopkins University in Baltimore, Maryland
Cell Membrane Fluidity
• To maintain proper function, a membrane’s lipids must be in optimum fluidity
• Too cold– a problem- could turn solid
• Too hot- a problem- could make membrane weak
Hydrocarbon Chains
• Chains can twist at the single bond sites
• This twisting increases as T increases
Solutions??• Develop some more unsaturated fats
– How would this help?
unsaturated
saturated
Solutions??• Get some cholesterol!
– How would this help?
cholesterol
phospholipid
Plant Cells
In plant cells, other steroids perform the same function as cholesterol
In plant cells, other steroids perform the same function as cholesterol
Exocytosis
The cell can create a vesicle that fuses with the membrane and then releases its contents outside of the cytosol
Endocytosis
The cell can take in nutrients by enclosing them in a vesicle and releasing the vesicle to the cytosol
Membrane Proteins• Integral Proteins- firmly bound to
the membrane• Amphipathic
– Hydrophilic regions extend outside the cell
– Hydrophobic regions intermingle with the fatty acid chains of the phospholipids
Transmembrane Protein
• Extend all the way through the cell membrane
• Some go through only once and some go through as many as 24 times
• The most common is -helix with hydrophobic amino acid side chains
Peripheral Proteins
• Located on the inner or outer surface of the membrane
• Usually bound to exposed parts of the integral proteins
Placement of Proteins in the Membrane
• As we know, there are proteins embedded in the membrane
• These proteins are not randomly placed in this location– They have a specific orientation with the
membrane
• There may be more proteins on the inside than the outside and vice versa
• As we know, there are proteins embedded in the membrane
• These proteins are not randomly placed in this location– They have a specific orientation with the
membrane
• There may be more proteins on the inside than the outside and vice versa
Membrane Protein Formation
• Inner surface proteins– Manufactured by free ribosomes– Move to the membrane through cytoplasm
and fuse with the membrane
Membrane Protein Formation
• Outer Surface Proteins– Formed by ribosomes on the
rough ER
Membrane Protein Formation• Outer Surface Proteins
– Become associated in ER membrane and push through into the lumen of the ER
• Here sugars are added that are found only in the lumen of the ER
• This makes them glycoproteins proteincarbohydrate
Membrane Protein Formation• Outer Surface
Proteins– Transport vesicles
move the newly formed gylcoprotein to the golgi complex
Golgi complex
Membrane Protein Formation• Outer Surface Proteins
– Glycoprotein becomes associated with the golgi complex in the same orientation as it did with the ER
• Further modification is made to the carbohydrate while it is in the lumen of the the golgi complex
• GC may also sort glycoproteins together
Membrane Protein Formation
• Outer Surface Proteins– GC buds and
sends vesicle to the plasma membrane where it fuses with the membrane
Membrane Protein Formation
• Outer Surface Proteins– The carbohydrate part of the glycoprotein
extends to the outside of the membrane
• Outer Surface Proteins– Formed by ribosomes on the rough ER– Become associated in ER membrane and push through into the
lumen of the ER• Here sugars are added that are found only in the lumen of the
ER• This makes them glycoproteins
– Transport vesicles move the newly formed gylcoprotein to the golgi complex
– Glycoprotein becomes associated with the golgi complex in the same orientation as it did with the ER
• Further modification is made to the carbohydrate while it is in the lumen of the the golgi complex
• GC may also sort glycoproteins together– GC buds and sends vesicle to the plasma membrane where it fuses
with the membrane– The carbohydrate part of the glycoprotein extends to the outside of
the membrane
How do Membranes Fuse?
• Golgi complex membrane is of same structure as the plasma membrane
• Fusion is like an air bubble with a speck of dirt in it rising to the surface of a glass of water and depositing the speck on the surface of the water as the water surrounding the bubble becomes part of the water’s surface.
Not
ice
any
sim
ilarit
ies?
Membrane Protein Functions