BioinformaticsIrfana Rashid

Bsc pharmacology

Ras9051.Uel.Ac.Uk

•Aim- To determine the function of potential protein products of a given DNA sequence. Firstly the nucleic acid translator is used to translate the

DNA sequence according to the triplet codon of the gene. The translator provided six possible translation peptide

products which when searched in the owl database provides a list of peptides that contain the sequence.

•The EC code from this database is noted and used to find information and structures of proteins from different organisms using the NCIB data base.

•All information on data bases and some DNA sequences can be found at

E.C code for the enzyme is - EC1.7.3.3

Polymer chain Residues

POLYMER CHAIN RESIDUES MOL-WEGHT CHAIN TYPE1UOX 296 33438 protien

This is a ribbion structure of the compound urate oxidase that the DNA sequence has coded.

Details on urate oxidase

• Title : Urate oxidase Aspergillus flavus complexed with its inhibitor 8 Azaxanthine.

•Classification: Oxedoreductase

•Biological unit: Tetramer

•Experiment method: X-ray diffraction

•Source: Aspergillus flavus

Urate oxidase and inhibitor complex

The gene coding for urate oxidase, an enzyme that catalyzes the oxidation of uric acid to allantoin, is inactivated in humans. Consequently, urate oxidase is used as a protein drug to overcome severe disorders induced by uric acid accumulation. Th structure of the active homotetrameric enzyme reveals the existence of a small architectural domain that we call T-fol (for tunnelling-fold) domain. It assembles to form a perfect unusual dimeric alpha 8 beta 16 barrel

Urate oxidase may be the archetype of an expanding new family of tunnel-shaped proteins that now has three members; tetrahydropterin synthase, GTP cyclohydrolase I and urate oxidase. The structure of the active site of urate oxidase around the 8-azaxanthine inhibitor reveals an original mechanism of oxidation that does not require any ions or prosthetic groups.