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Ensemble or group of glycan structures resulting from coordinated expression of several biosynthetic enzymes
Non-template biosynthesis
Challenges
• Chemical heterogeneity • Challenges in isolation and analysis• Challenges in representation/processing glycan Information
Multivalent interactions involving multiple glycan motifs with multivalent CRDs on proteins
Glycan-Protein InteractionGlycan
Structure-FunctionRelationships
Challenges
• Understanding biochemical basis for glycan-protein interactions• How glycan specific genotype governs whole organism phenotype• Constructing biological pathways and interaction networks
Advancing GlycomicsAdvancing GlycomicsKey Issues & ChallengesKey Issues & Challenges
Consortium for Functional Consortium for Functional GlycomicsGlycomics
www.functionalglycomics.orgwww.functionalglycomics.org
• Active collaborative effort to advance glycomics
• Goal: Understand role of glycan–protein interactions in cell-cell communication
• Develop technologies and resources to obtain data at various levels from molecule mouse
• Integrated approach to glycan structure-function
Bioinformatics Core (B)Bioinformatics Core (B)
Vision & Approach
• Vision: Present the dynamic “face” of the Consortium via Internet to best utilize the value of the resources and datasets generated by its participants and the broader scientific community.
Object-based Relational Database implemented using
Oracle
Middleware implemented using Entity-Class
Operations, Servlets in Java
Static Pages (SHTML) for
Web Site
Dynamic pages (JSPs) for database driven
Entry, Dissemination and Queries
BIOFUNCTION_CATEGORY
Biofunction_key: INTEGER
Biofunction_name: VARCHAR2(20)
BIOLOGICAL_SOURCE
biosource_key: INTEGER
Biosource_ID: VARCHAR2(20)kingdom: VARCHAR2(20)Phylum: VARCHAR2(20)Class: VARCHAR2(20)Genus: VARCHAR2(20)Species: VARCHAR2(20)
CARBOHYDRATE
Carb_key: INTEGER
biosource_key: INTEGERCarb_ID: VARCHAR2(20)Carb_Sciname: VARCHAR2(20)Carb_commonName: VARCHAR2(20)Carb_Family: VARCHAR2(20)Carb_Subfamily: VARCHAR2(20)Carb_Type: VARCHAR2(10)Carb_MW: NUMBERBiofunction_key: NUMBERCarb_BiofunctionDesc: VARCHAR2(100)Carb_listdate: DATECarb_structure: VARCHAR2(500)Carb_structureImgtype: VARCHAR2(10)Carb_structureImg: BLOBAccess_code: VARCHAR2(20)
DNASEQ
DNASEQ_key: INTEGER
Gene_key: INTEGERDNASEQ_ID: VARCHAR2(20)DNASEQ_IDtype: VARCHAR2(20)DNASEQ_sequence: VARCHAR2(20)
GENE
Gene_key: INTEGER
Genome: VARCHAR2(20)Gene_name: VARCHAR2(20)biosource_key: INTEGER
PAPER
Paper_key: NUMBER
Paper_ID: VARCHAR2(20)ID_type: VARCHAR2(20)Paper_title: VARCHAR2(200)Paper_year: INTEGERJournal_name: VARCHAR2(200)Page_range: VARCHAR2(20)
PAPER_REFERENCE
Paper_key: NUMBERCarb_key: INTEGER
PROTEIN
Protein_key: INTEGER
Protein_ID: VARCHAR2(20)ProteinID_type: VARCHAR2(20)Protein_name: VARCHAR2(20)Protein_CommonName: VARCHAR2(20)Protein_family: VARCHAR2(20)Protein_subFamily: VARCHAR2(20)Protein_superUnitKey: INTEGERProtein_type: VARCHAR2(20)Biosource_key: INTEGERBiofunction_key: INTEGERGene_key: VARCHAR2(20)DNASEQ_key: INTEGERProtein_sequenceKey: INTEGERProtein_biofunctionDesc: VARCHAR2(20)Access_code: VARCHAR2(20)Protein_listdate: DATE
PROTEINSEQUENCE
Protein_sequenceKey: INTEGER
Protein_sequence: VARCHAR2(20)Protein_key: INTEGER
REAGENT
Reagent_key: INTEGER
Reagent_ID: VARCHAR2(20)Reagent_IDtype: VARCHAR2(20)Reagnet_name: VARCHAR2(20)Reagent_formula: VARCHAR2(20)Reagent_MW: INTEGER
CPBINDING
Cpb_key: NUMBER
Cpb_ID: VARCHAR2(20)Cpb_IDtype: VARCHAR2(20)Carb_key: NUMBERProtein_key: NUMBERCpb_flag: CHAR(1)Biofunction_key: NUMBERCpb_BiofunctionDesc: VARCHAR2(100)Cpb_Keq: NUMBERCpb_DG: NUMBERCpb_DH: NUMBERCpb_DS: NUMBERCpb_listdate: DATE
PROTOCOL
Protocol_key: INTEGER
Protocol_ID: VARCHAR2(20)Protocol_Category: VARCHAR2(20)Protocol_keyword: VARCHAR2(20)Protocol_Title: VARCHAR2(20)Protocol_overview: VARCHAR2(20)Protocol_procedure: CHAR(18)Protocol_trobleshooting: VARCHAR2(20)Dbuser_key: INTEGER
CPATHREAGENT
Protocol_key: INTEGERReagent_key: INTEGER
Reactant_Weightg: INTEGERReactant_mmol: VARCHAR2(20)
CPATHINSTRUMENT
Protocol_key: INTEGERInstrument_key: INTEGER
INSTRUMENT
Instrument_key: INTEGER
Instrument_category: VARCHAR2(20)Instrument_name: VARCHAR2(20)Instrument_model: VARCHAR2(20)Instrument_Vender: VARCHAR2(20)
CARBPROFILE
Carbprofile_key: NUMBER
Carbprofile_ID: VARCHAR2(20)Carbprofile_title: VARCHAR2(30)Carbprofile_carbComp: VARCHAR2(200)Carbprofile_avgMW: NUMBERMouseind_key: NUMBERCarbprofile_source: VARCHAR2(100)Biofunction_key: NUMBERCarbprofile_biofunctionDesc: VARCHAR2(200)Parentprofile_ID: VARCHAR2(20) CPROFILEID
Carbprofile_key: NUMBERCarb_key: INTEGER
Concentration: VARCHAR2(20)CPROFILEEXP
Cpexp_key: INTEGER
cpexp_ID: VARCHAR2(20)cpexp_title: VARCHAR2(20)Protocol_key: INTEGERCarbprofile_key: NUMBERCpexp_keyword: VARCHAR2(20)Cpexp_instrument: VARCHAR2(20)Cpexp_date: DATECpexp_comment: VARCHAR2(20)Dbuser_key: INTEGER
MICROARRAY
Marray_key: INTEGER
Marray_ID: VARCHAR2(20)Marray_type: VARCHAR2(20)Marray_desc: VARCHAR2(20)Marray_imgtype: VARCHAR2(20)Marray_img: BLOB
MOUSESTRAIN
Mouse_key: INTEGER
Mouse_ID: VARCHAR2(20)Cell_key: NUMBERMouse_type: VARCHAR2(20)Protocol_key: INTEGERMouse_keyword: VARCHAR2(20)Mouse_desc: VARCHAR2(20)
KOGENE
KOgene_key: INTEGER
KOgene_ID: VARCHAR2(20)Mouse_key: INTEGERGene_key: INTEGERGene_ID: VARCHAR2(20)KOgene_synonym: VARCHAR2(20)KOgene_Mtype: VARCHAR2(20)KOgene_UnigenDes: VARCHAR2(20)KOgene_HumanGene: VARCHAR2(20)
CELLSOURCE
Cell_key: NUMBER
Cell_ID: VARCHAR2(20)biosource_key: NUMBERCell_line: VARCHAR2(30)Cell_linetype: VARCHAR2(20)Cell_tissue: VARCHAR2(20)Cell_Organ: VARCHAR2(20)
MAEXP
Maexp_key: INTEGER
Maexp_ID: VARCHAR2(20)Maexp_title: VARCHAR2(20)Protocol_key: INTEGERMarray_key: INTEGERMaexp_SampleID: VARCHAR2(20)Maexp_SampleDesc: VARCHAR2(20)Maexp_keyword: VARCHAR2(20)Maexp_readtype: VARCHAR2(20)Maexp_raw: VARCHAR2(20)Maexp_date: DATEMaexp_imgfiletype: VARCHAR2(20)Maexp_imgraw: BLOBDbuser_key: INTEGER
TARGETGENE
Maexp_key: INTEGERGene_key: INTEGER
Targetgene_desc: VARCHAR2(20)
PROTIENREF
Paper_key: NUMBERProtein_key: INTEGER
CARBPATHREF
Paper_key: NUMBERCpath_key: INTEGER
PROTOCOLREF
Paper_key: NUMBERProtocol_key: INTEGER
CPBREF
Paper_key: NUMBERCpb_key: NUMBER
DBUSER
Dbuser_key: INTEGER
Dbuser_username: VARCHAR2(20)Dbuser_password: VARCHAR2(20)Dbuser_lastname: VARCHAR2(20)Dbuser_firstname: CHAR(18)Dbuser_email: VARCHAR2(20)Dbuser_employer: VARCHAR2(20)Dbuser_title: VARCHAR2(20)Dbuser_Core: VARCHAR2(20)Dbuser_listdate: DATE
MOUSEHEMA
MH_key: INTEGER
MH_ID: VARCHAR2(20)Mouseind_key: INTEGERMH_date: DATEMH_age: INTEGERMH_weight: INTEGERMH_WBC: INTEGERMH_Neutrophils: INTEGERMH_Lymphocytes: VARCHAR2(20)MH_HGB: INTEGERMH_HCT: INTEGERMH_PLT: INTEGERMH_MPV: INTEGERDbuser_key: INTEGER
MOUSEHISTOLOGY
MHS_key: INTEGER
MHS_ID: VARCHAR2(20)Mouseind_key: INTEGERMHS_date: DATEMHS_age: INTEGERMHS_weight: INTEGERProtocol_key: INTEGERMHS_purpose: VARCHAR2(20)MHS_result: VARCHAR2(20)MHS_comment: VARCHAR2(20)Dbuser_key: INTEGER
MOUSEIMMU
MI_key: INTEGER
MI_ID: VARCHAR2(20)Mouseind_key: INTEGERMI_date: DATEMI_age: INTEGERMI_weight: INTEGERMI_sampletype: VARCHAR2(20)MI_CD4: INTEGERMI_CD8: INTEGERB220: INTEGERIAB: INTEGERIgM: INTEGERIgD: INTEGERDbuser_key: INTEGER
MOUSEIND
Mouseind_key: INTEGER
MIND_ID: VARCHAR2(20)Mouse_key: INTEGERMind_sex: VARCHAR2(20)
MOUSEBEHAV
MB_key: INTEGER
MB_ID: VARCHAR2(20)Mouseind_key: INTEGERMB_date: DATEMB_age: INTEGERMB_weight: INTEGERMB_GrossNero: VARCHAR2(20)MB_SensorMotorReflex: VARCHAR2(20)MB_PosturalReflex: VARCHAR2(20)MB_motoractivity: VARCHAR2(20)MB_Nocieption: VARCHAR2(20)MB_AcousticStartle: VARCHAR2(20)MB_SocialDomine: VARCHAR2(20)MB_conditionedFear: VARCHAR2(20)MB_waterMaze: VARCHAR2(20)MB_radialArmMaze: VARCHAR2(20)Dbuser_key: INTEGER
MOUSEMETABOLISM
MM_key: INTEGER
MM_ID: VARCHAR2(20)Mouseind_key: CHAR(18)MM_date: DATEMM_age: INTEGERMM_weight: INTEGERMM_Cardiac: VARCHAR2(20)MM_BloodDiastol: INTEGERMM_bloodSystol: INTEGERMM_plumonary: VARCHAR2(20)MM_FoodConsumption: INTEGERMM_waterConsumption: INTEGERMM_activityLevel: INTEGERMM_OxygenConsumption: INTEGERMM_CO2Output: INTEGERMM_HeatOutput: INTEGERDbuser_key: INTEGER
MAREF
Marray_key: INTEGERPaper_key: NUMBER
ENZYME
Enzyme_key: INTEGER
Enzyme_ID: VARCHAR2(20)Enzyme_type: VARCHAR2(20)Enzyme_name: VARCHAR2(20)Enzyme_CommonName: VARCHAR2(20)Enzyme_family: VARCHAR2(20)Enzyme_subFamily: VARCHAR2(20)biosource_key: INTEGERBiofunction_key: INTEGEREnzyme_biofunctionDesc: VARCHAR2(20)Gene_key: VARCHAR2(20)DNASEQ_key: INTEGEREnzyme_sequenceKey: INTEGERProteindb_ID: VARCHAR2(20)SwissPort_ID: VARCHAR2(20)Access_Code: VARCHAR2(20)Protein_listdate: DATE
CARBPATHWAY
Cpath_key: INTEGER
Cpath_name: VARCHAR2(20)Carb_key: INTEGEREnzyme_key: INTEGERCpath__Reaction: VARCHAR2(20)Cpath_condition: VARCHAR2(20)Cpath_stepFromCarb: INTEGERCpath_yield: INTEGER
ENZYMESEQUENCE
Enzyme_sequenceKey: INTEGER
Enzyme_sequence: VARCHAR2(20)Enzyme_key: INTEGER
CARBEXP
Carbexp_key: INTEGER
carbexp_ID: VARCHAR2(20)carbexp_title: VARCHAR2(30)Protocol_key: NUMBERCarb_key: NUMBERCarbexp_keyword: VARCHAR2(100)Carbexp_Instrument: VARCHAR2(100)Carbexp_comment: VARCHAR2(200)Carbexp_date: DATECabexp_batchno: VARCHAR2(20)User_key: NUMBER
CARBEXPIMG
carbExpImg_key: INTEGER
Carbexp_key: NUMBERCarbExpImg_exptype: VARCHAR2(20)CarbExpImg_filetype: VARCHAR2(20)CarbExpImg_imageRaw: BLOB
CPBEXP
Cpbexp_key: NUMBER
cpbexp_ID: VARCHAR2(20)cpbexp_title: VARCHAR2(20)Protocol_key: INTEGERCpb_key: NUMBERCpbexp__keyword: VARCHAR2(20)Cpbexp_Instrument: VARCHAR2(20)Cpbexp_date: DATECpbexp_comment: VARCHAR2(20)Dbuser_key: INTEGER
CPBEXPIMG
cpbExpImg_key: INTEGER
Cpbexp_key: NUMBERCpbexpImg_exptype: VARCHAR2(20)CpbexpImg_imageRaw: BLOBCpbexpimg_desc: VARCHAR2(20)CpbexpImg_filetype: VARCHAR2(20)
CPROFILEEXPIMG
cpexpImg_key: INTEGER
Cpexp_key: INTEGERCpexpImg_exptype: VARCHAR2(20)CpexpImg_filetype: VARCHAR2(20)CpexpImg_imageRaw: BLOBCpexpImg_desc: VARCHAR2(20)
MAEXPANALYSIS
Maexpanaly_key: INTEGER
Maexp_key: INTEGERMaexpanaly_title: VARCHAR2(100)Maexpanaly_desc: VARCHAR2(500)
CBPEXP
Cbpexp_key: INTEGER
Cbpexp_ID: VARCHAR2(20)Cbpexp_title: VARCHAR2(20)Protocol_key: INTEGERProtein_key: INTEGERCbpexp_keyword: VARCHAR2(20)Cbpexp_Instrument: VARCHAR2(20)Cbpexp_comment: VARCHAR2(20)Cbpexp_date: DATEDbuser_key: INTEGER
ENZEXPIMG
EnzexpImg_key: INTEGER
Enzexp_key: INTEGEREnzexpImg_exptype: VARCHAR2(20)EnzexpImg_filetype: VARCHAR2(20)EnzexpImg_imageRaw: BLOB
ENZEEXP
Enzexp_key: INTEGER
Enzexp_ID: VARCHAR2(20)Enzexp_title: VARCHAR2(20)Protocol_key: INTEGEREnzyme_key: INTEGEREnzexp_keyword: VARCHAR2(20)Enzexp_Instrument: VARCHAR2(20)Enzexp_comment: VARCHAR2(20)Enzexp_date: DATEUser_key: INTEGER
CBPEXPIMG
CbpexpImg_key: INTEGER
Cbpexp_key: INTEGERCbpexpImg_exptype: VARCHAR2(20)CbpexpImg_filetype: VARCHAR2(20)CbpexpImg_imageRaw: BLOB
GLYCOARRAY
Garray_key: INTEGER
Garray_ID: VARCHAR2(20)Garray_type: VARCHAR2(20)Garray_desc: VARCHAR2(20)Garray_imgtype: VARCHAR2(20)Garray_img: BLOB
GAEXP
Gaexp_key: INTEGER
Gaexp_ID: VARCHAR2(20)Gaexp_title: VARCHAR2(20)Protocol_key: INTEGERGarray_key: INTEGERGaexp_SampleID: VARCHAR2(20)Gaexp_SampleDesc: VARCHAR2(20)Gaexp_keyword: VARCHAR2(20)Gaexp_readtype: VARCHAR2(20)Gaexp_raw: VARCHAR2(20)Gaexp_date: DATEGaexp_imgfiletype: VARCHAR2(20)Gaexp_imgraw: BLOBDbuser_key: INTEGER
TARGETPROTEIN
Gaexp_key: INTEGERProtein_key: INTEGER
Targetcbp_desc: VARCHAR2(20)
CPATHEXP
Cpathexp_key: INTEGER
Cpathexp_ID: VARCHAR2(20)Cpathexp_title: VARCHAR2(20)Cpath_key: INTEGERProtocol_key: INTEGERCpathexp_keyword: VARCHAR2(20)Cpathexp_Instrument: VARCHAR2(20)Cpathexp_comment: VARCHAR2(20)Cpathexp_date: DATEUser_key: INTEGER
CPATHEXPIMG
CpathexpImg_key: INTEGER
Cpathexp_key: INTEGERCpathexpImg_exptype: VARCHAR2(20)CpathexpImg_filetype: VARCHAR2(20)CpathexpImg_imageRaw: BLOB
CARBINVENTORY
Carbinv_key: NUMBER
Carbinv_ID: VARCHAR2(20)Carb_key: NUMBERCarbexp_key: VARCHAR2(20)Carbinv_Purity: NUMBERCarbinv_color: VARCHAR2(10)Carbinv_State: VARCHAR2(10)Quantity: NUMBERCarbexp_batchno: VARCHAR2(20)
CARBORDER
Corder_key: NUMBER
Corder_ID: VARCHAR2(20)Corder_date: DATECarbinv_key: NUMBEROrder_receiver: VARCHAR2(50)Order_address: VARCHAR2(200)Order_quantity: NUMBERshipping_flag: CHAR(1)
Part of CFG database overall ontology map
Core B OrganizationCore B Organization
Core Operations &
Management
BioinformaticsScientific liaisons, User specifications,
Bioinformatics applications
Information TechnologyDatabase, web, software
applications, user interface development
AdministrationRam Sasisekharan (Core Coordinator), Rahul Raman (Core Director)
Ada Ziolkowski (Admin Staff)
Core B Team Maha Venkataraman, Subu Ramakrishnan, Savitri Subramanian, Thomas Lutteke
Ganesh Venkataraman, Wei Lang, Eric Berry, Nishla Keiser, Ishan Capila, Chipong Kwan and Consultants
MIT Information Systems
Server Maintenance
MIT Information Systems
Database Administration
Consortium DB
Data Objects, Relationships, Raw and Parsable Data
Public Databases
GBP, Glycans, Glycoenzymes
User InterfaceData Acquisition
Web-based Forms Annotation Tools
User Interface for InformationDissemination
GBP Molecule
Pages
GlycoEnzyme Molecule
Pages
Glycan StructuresDatabase
Consortium Data
Dissemination
Implementation StrategyImplementation StrategyDatabases and Interfaces
Implementation StrategyImplementation StrategySchema of CFG Data Objects and Integration
Glycobiology, 16(5), 82R-90R
Highlights of ProgressHighlights of ProgressYears 1-4
• Infrastructure set-up
• Database implementation
• Software development
• Interactions with Cores for data acquisition and dissemination
Years 1 - 2• Acquisition of Core data
• Public release of databases
• CFG data dissemination
• GBP molecule pages
• Glycan structures DB
Years 3-4
Highlights of ProgressHighlights of ProgressQuarters 1-3 of Year 5
CFG data acquisition and dissemination• Streamlining the process to upload printed array data from Cores D and H• Enhancing interfaces for disseminating scientific Core data• Implementation of data dissemination and research tracking interfaces for PIs
Specialized databases and molecule pages• Data organization and implementation of glycosyltransferase database• Updating expert contribution fields for C-type lectins in GBP database
Other key highlights• Bioinformatics satellite session at the Society for Glycobiology 2005 meeting –
discussion of glycan analysis methods and data exchange formats• Renewal application and preparation for Council review
Publications• “Glycomics: An integrated systems approach to structure-function relationships of glycans.”
Nature Methods, 2(11): 817-24• “Advancing glycomics: implementation strategies at the consortium for functional glycomics.”
Glycobiology, 16(5): 82R-90R
CFG Scientific Core DataCFG Scientific Core DataData Organization
Experiment Information
Brief description of scope of experiment or analysis
Experiment Information
Brief description of scope of experiment or analysis
Protocols
Standardized Protocols used in an experiment or
analysis
Protocols
Standardized Protocols used in an experiment or
analysis
Sample/Resources
Detailed information on sample / resource utilized [mouse, tissue, cell, GBP]
Sample/Resources
Detailed information on sample / resource utilized [mouse, tissue, cell, GBP]
Data Summary
Summary of interpretation of data by Scientific Core
Data Summary
Summary of interpretation of data by Scientific Core
Raw Data
Raw data files generated by the analysis – Images, Excel,
binary formats
Raw Data
Raw data files generated by the analysis – Images, Excel,
binary formats
Annotated Data
Processed information on entities such as gene, mouse, GBP, etc. stored in DB tables
Annotated Data
Processed information on entities such as gene, mouse, GBP, etc. stored in DB tables
CFG Scientific Core DataCFG Scientific Core Data Statistics (as of June 2006)
Gene Microarray (E)
47 Experiments (33)
617 Samples (387)
3546 data files (1826)
Mouse Phenotyping (G)
16 KO Strains (11)
266 Experiments (149)
3116 data files (1100)
Glycotechnology (C)
Mouse: 11 tissues, 8 KO strains, 92 MALDI-MS spectra
Human: 11 Tissues 108 MALDI-MS spectra
Cell Lines: 12 Cell Lines 27 Spectra
GBP-Glycan (H)
247 Samples (143)
CFG Scientific Core DataCFG Scientific Core DataEnhanced dissemination interfaces
Navigation and downloading of CFG Data – Gene Expression, MALDI-MS Glycan Profiling, Mouse Phenotyping and Glycan Array - DEMO
CFG Data IntegrationCFG Data IntegrationGlycan profile of spleen of FucT-VII KO mice with differences in histology staining
Tracking PI Research ProgressTracking PI Research ProgressData dissemination and resource tracking
interface
PI
Core A
Database
Request ID Data
IDs
Core
Submit Online Request Form
Copy of submitted request emailed with Request ID in database
SC
Post Status
Prioritizing/ Approved
Request
Approval Process
Upload Datawith Request ID
Tracking PI Research Tracking PI Research ProgressProgress
Data dissemination and resource tracking interface
Navigating PI information page to find associated resource requests and data - DEMO
Automated Acquisition
Data from public databases, links to public resources
Data from Cores
Interface to CFG resources and data
Expert Contribution
Filling out fields as experts on the molecule
MoleculeInformation
Portal
GBP Molecule Page GBP Molecule Page InterfacesInterfaces
Three main componentsThree main components
• Glycan array data
• Mouse phenotyping data
• Transgenic mouse line
Contribution from experts obtained for C-type lectins
GBP Molecule PagesGBP Molecule PagesUpdating expert contributions for C-type Updating expert contributions for C-type
lectinslectins
GBP Molecule Page example for C-type lectins with filled expert contribution fields - DEMO
DC-SIGN Molecule PageDC-SIGN Molecule Page
PDB IDs of DC-SIGNcomplexed with glycanligands identified usingPDB2LINUCS tool
ExpertContributions
GT Database DevelopmentGT Database DevelopmentExpert annotation of glycosylation Expert annotation of glycosylation
pathwayspathwaysType II Extension/ TerminationComposite Structure
FucTs expert annotation
343 Genes
40 Sialyl-T24 Fucosyl-T69 GlcNAc-T 47 Gal-T 54 GalNAc-T 78 Sulfo-T 31 Man-T
Automated Acquisition
Data from public databases, links to public resources
Data from Cores
Interface to CFG resources and data
MoleculeInformation
Portal
GT Database DevelopmentGT Database DevelopmentGlycosylation interface & GT molecule Glycosylation interface & GT molecule
pagespages
• Glycan profiling of GT KO mice
• Mouse phenotyping data
• Transgenic mouse line
• Gene expression (Year 6 goals)