Slide 1

Bioinformatics BIO520 Chuck Staben Tom Badgett

Slide 2

Louis XV After me, the deluge

Slide 3

Bioinformatics-what is it? Acquisition, curation, and analysis of biological data Hypothesis

Slide 4

Bioinformatics training Who needs it? Life Scientists Professional Graduate Undergraduate Medical professionals Pre-med undergrads Information scientists Computer scientists Library/database specialists Statisticians Mathematicians

Slide 5

BIO520 Syllabus Content Lectures Lab Exercises: sessions or NetMeetings Reading www.uky.edu/Classes/BIO520/BIO520WWW/ UKTV (16): Sat, Sun 4-5 PM Expectations Grading Assignments, Hour Exam, Project

Slide 6

Class Project Fermentation-Respiration Shift in Yeast Transcription analysis Control sequences Transcription/Translation/Metabolism Correlation Evolutionary Conservation Applications to Drug Discovery

Slide 7

Computer Resources WWW materials www.uky.edu/Classes/BIO520/BIO520WWW/ e-mail listserv: BIO520@lsv.uky.edu newsgroup: news.uky.edu/uky.classes.bio520 NetMeeting SeqWeb www.seqanal.mi.uky.edu Seqanal seqanal.mi.uky.edu (telnet)

Slide 8

Information Science Dramatic Changes Information Storage Digitized databases: text, numbers, images Computerized Analysis Facile Information Distribution WWW, e-mail,...

Slide 9

Bioinformatic Data-1978 to 1998 DNA sequence Gene expression Protein expression Protein Structure Genome mapping Metabolic networks Regulatory networks Trait mapping Gene function analysis Scientific Literature

Slide 10

DNA Sequence

Slide 11

Transcript Analyses Northern Blot-1977Genomic transcript profiling DeRisi, Iyer, Brown; Science, 1998

Slide 12

Protein Structure Determination

Slide 13

Genome/Trait Maps

Slide 14

Protein Expression Analysis 1978-1998

Slide 15

Metabolic Networks KEGG, 1998

Slide 16

Regulatory Networks KEGG

Slide 17

Bioinformatic Challenges Acquire, store, share, search data Analyze data Interpret analyses Design further analyses to produce high-value information Apply results of analyses

Slide 18

Biological Principles Structure Function Homeostasis Evolution by natural selection

Slide 19

Biological Hierarchies Molecule Cell Tissue Organ Organism Population Species Biosphere