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S704 Abstracts / Journal of Biotec

VII5-P-062

Biodegradation of PCP in soil by bioaugmentation method withwhite-rot fungus Coriolus versicolor

Kazuhiro Hoshino ∗, Rie Watanabe, Maki Takano

Graduate School of Science and Technology, University of Toyama,Toyama 930-8555, Japan

E-mail address: khoshino@eng.u-toyama.ac.jp (K. Hoshino).

In soil purification, the bioaugmentation which pours degrading-bacteria into the pollution spot is a suitable technology (Lestanand Lamar, 1996). The method can be also applied to the pollutionspot with very few or no degrading-bacteria and the field to whichself-purification ability is weak. However, the application in spotis hardly done because the safety of the foreign bacteria pouredinto it and its behaviour after the injection in the spot can’t bequite predicted. The detection and quantification of such a specificmicroorganism is not easy in soil. In this research, with a secretionsystem of laccase by the white-rod fungus C. versicolor (Ullah et al.,2000; Ford et al., 2007), we examined the development of bioaug-mentation method that a fungus is injected in soil contained bypentachlorophenol (PCP). We examined the relations of the growthof fungus and the secretion enzyme in the polluted soil filling upthe soil column (ID: 75 mm, H: 40 cm). Mycelia were inoculated atthe position of a depth of 0–5 cm from the surface, so that it mightbecome uniform. First, the growth of fungus showed the logarith-mic growth until 8 day and then was decreased rapidly. The laccaseactivity in the soil rose after 8th day and then the activity was keptat the almost same level until the end of the cultivation. Moreover,in the depth from 0 to 10 cm PCP in was degraded smoothly, andthen the concentration was decreased from 84 ppm to 23 for 40days. In conclusion, when the detection and quantitative estima-tion of a specific fungus injected in soil could attempt by Real-timePCR method, the growth could be grasped in the soil through thisresearch. Moreover, by using soil column the behaviour of the fun-gus could be conformed such as its growth, secretion of laccase, anddegradation of PCP.

References

Ford, C.I., Walter, M., Northcott, G.L., Di, H.J., Cameron, K.C., Trower, T., 2007. Fungalinoculum properties: extracellular enzyme expression and pentachlorophenolremoval in highly contaminated field soils. J. Environ. Qual. 36, 1599–1608.

Lestan, D., Lamar, R.T., 1996. Development of fungal inocula for bioaugmentation of

contaminated soils. Appl. Environ. Microbiol. 62, 2045–2052.

Ullah, M.A., Bedford, C.T., Evans, C.S., 2000. Reactions of pentachlorophenol withlaccase from Coriolus versicolor. Appl. Microbial. Biotechnol. 53, 230–234.

doi:10.1016/j.jbiotec.2008.07.1633

VII5-P-063

Synergy effect on bioremediation by using co-culturing effec-tive microorganisms with Sphingobacterium sp. WY

Ki-Seok Yoon 1,∗, Seok-Young Moon 1, Sun-ung Heo 1, Hyun-ShikYun 1, Young-Jun Kim 2, Yoon-Mo Koo 3

1 Department of Biological Engineering, Inha University, Incheon 402-751, Republic of Korea2 ERC for Advanced Bioseparation Technology, Inha University, Incheon402-751, Republic of Korea3 Department of Biological Engineering, ERC for Advanced Biosepara-tion Technology, Inha University, Incheon 402-751, Republic of Korea

Effective Microorganisms (EM) (Higa and Wididana, 1991) consistof eighty different species. Especially there are three main gen-

gy 136S (2008) S678–S707

era: phototrophic bacteria, lactic acid bacteria, and yeast. Recentstudies about EM show that it can degrade the petroleum concen-tration of the petroleum contaminated soil. Sphingobacterium sp.WY (Park, 2002) was isolated in petroleum contaminated site andit also can degrade the petroleum concentration. The final objec-tive of this study is to apply both EM and Sphingobacterium sp. WYinto oil-contaminated site for inexpensive bioremediation. Acrossthe study of the cell interaction, co-culture is obviously necessary.Thus, as the beginning stage, we have examined the symbiosisbetween EM and Sphingobacterium sp. WY by co-culturing of bothof them. Composition of culture medium and condition was: glu-cose 20 g l−1, MgSO4 1.2 g l−1, Yeast extract 20 g l−1, KH2PO4 5 g l−1,K2HPO4 3 g l−1 and 30 ◦C, 150 rpm in shaking incubator for 72 h.During the culture, Optical density was measured by spectropho-tometer at 600 nm to analyze the cell growth and the symbiosis wasobserved by using an optical microscope. The result of co-cultureshowed higher OD 600 value than each culture, and they can livecommensally. The synergy effect of EM and Sphingobacterium sp.WY on their petroleum-degrading activities was also investigated.

References

Ghazali, Farinazleen Mohamad, Rahman, Raja Noor Zaliha Abdul, Salleh, Abu Bakar,Basri, Mahiran, 2004. Biodegradation of hydrocarbons in soil by microbial con-sortium. Int. Biodeter. Biodegr. 54, 61–67.

Higa, T., Wididana, G.N., 1991. Concept and theories of effective microorganisms.In: Parr, J.F., Hornic, S.B., Whitman, C.E. (Eds.), Proceedings of the First Interna-tional Conference on Kyusei Nature Farming. U.S. Department of Agriculture,Washington, DC, USA, pp. 118–124.

Park, C.-B., 2002. Biodegradation of diesel oil by microorganisms isolated frompetroleum contaminated site.

doi:10.1016/j.jbiotec.2008.07.1634

VII5-P-064

Comparison of toluene removal in biofilters packed with fourdifferent media

Hee Wook Ryu 1,∗, Kyung-Suk Cho 2

1 Department of Chemical and Environmental Engineering, SoongsilUniversity, Seoul 156-743, South Korea2 Department of Environmental Science and Engineering, Ewha Wom-ans University, Seoul 120-750, South Korea

E-mail addresses: hwryu@ssu.ac.kr (H.W. Ryu), kscho@ewha.ac.kr

(K.-S. Cho).

Biofiltration technology has been used for the removal of relativelylow concentrations of volatile organic compounds due to its simpleoperation and lower expense (Kim et al., 2005; Shim et al., 2006). Inthis study, to select the best available filter material, we comparedthe efficiency of toluene removal by biofilters packed with differentfilter materials, including porous ceramic (celite) (Lee et al., 2005),Jeju scoria (lava) (Cho et al., 2000), a mixture of granular activatedcarbon and celite (GAC/celite), and cubic PU foam (Kim et al., 2005;Shim et al., 2006). Toluene removal in biofilters packed with celite,lava, GAC/celite, and PU is shown in Fig. 1. The maximum elimina-tion capacities in the celite, lava, and GAC/celite biofilter were 100,150, and 110 g m−3 h−1, respectively. In contrast, the eliminationcapacity for the PU biofilter was approximately 350 g m−3 h−1 atan inlet loading of approximately 430 g m−3 h−1, which was 2–3.5times higher than for the other biofilters. After 15 to 20 d, when thepressure drop in the celite, lava, and GAC/celite biofilters began toincrease, the elimination efficiency decreased. After 30–40 d, whenthere was a dramatic increase in the pressure drop, the elimina-tion efficiency decreased to 40–65%. However, the PU filter had thehighest removal efficiency and lowest pressure drop. Comparison