Biocontrol

Justin Kim

Need

Knowledge Base

Jorge M. Vivanco (2002)Harsh Pal Bais (2000)

R. Charudattan (2006)R. Charudattan (2006)

Biocontrol

Biocontrol Agent

• (Elizabeth and Emmert, 1999).

Bacillus Subtilis

Kung-Ta Lee (1993) Kung-Ta Lee (2001)

Literature Review

Fall, Ray (2004)

Disease

Ross Ballard (2008)

Jason Brantner (2004)

Arabidopsis/SoybeanN= 120

Control Group-Seeds grown in normal environmentN=30

V3- Plant inoculated with Pseudomonas Putida from fusariumN=30

V2- Plant inoculated with bacillus subtilis from the fusariumN=30

Mortality of the plants are comparedMean +- SDTwo way Anova

V1- plant grown with the fusariumN=30

Methodology Flow Chart

Do-Ability

• Biocontrols available from Online Carolina Catalog

Purpose-

To find biocontrol agents that works better against certain diseases

Hypothesis-Alternative- Bacillus subtilis worked better against the disease

Alternative- Pseudomonas putida worked better against the disease

Budget PlanVendor Item # Quantity Description Price Total

Carolina Online 154921 1 Bacillus subtilis, Living, Tube $9.20 $9.20

Carolina Online 156033 1 Fusarium Oxysporum $9.20 $9.20

Carolina Online 177600 1 Arabidopsis: Columbia (Col-0) Seed, Pk 200 $10.50 $10.50

Carolina Online 593120 1 Walter QZG Zoom Stereomicroscope $519.00 $519.00

BD CATALOG 442104 1 Diluting fluid- used to count Bacteria

Carolina Online 741270 1 Petri Dish, Polymethylpentene, 100 x 15 mm, each $6.45 $6.45

Carolina Online 195700 1 Murashige and Skoog Salt Base, Powdered, 10 x 1 L $16.05 $16.05

Carolina Online 155265 1 Pseudomonas putida, Living, Tube $9.20 $9.20

Bibliography

• Baker et al., "Inhibitory effect of Bacillus subtilis on Uromyces phaseoli and on development of rust pustules on bean leaves" Phytopathology 73:1148-1152 (1983).

• Cavaglieri L., Orlando J., Rodríguez M.I. - Biocontrol of Bacillus subtilis against Fusarium verticillioides in vitro and at the maize root level- Departamento de Microbiología e Inmunología, Facultad de Ciencias Exactas, Físico-Químicas y Naturales, Universidad Nacional de Río Cuarto, Ruta Nacional 36 Km, 601, (5800) Río Cuarto, Córdoba, Argentina

• Elizabeth AB, Emmert HJ. Biocontrol of plant disease: A (Gram-)positive perspective. FEMS Microbiol Lett. 1999;171:1–9

• Estruch et al., "Transgenic plants: An emerging approach to pest control" Nature Biotechnology 15:137-141 (1997).• G. Heidi . Elnaga, Abo - Bacillus subtilis as a Biocontrol Agent for Controlling Sugar Beet Damping-off Disease -Egypt.

J. Phytopathol., Vol. 34, No.1, pp. 51-59 (2006)• Olsson J, Bőrjesson T, Lundstedt T, Schnürer J. Detection and quantification of ochratoxin A and deoxynivalenol in

barley grains by GC-MS and electronic nose. Int J Food Microbiol. 2002;72:203–214.• Onji Y, Aoki Y, Tani N, Umebayashi K, Kitada Y, Dohi Y. Direct analysis of several Fusarium mycotoxins in cereals by

capillary gas chromatography-mass spectrometry. J Chromatogr A. 1998;815:59–65.• Placinta CM, D’Mello JPF, Macdonald AMC. A view of worldwide contamination of cereal grains and animal feed

with Fusarium mycotoxins. Anim Feed Sci Tech. 1999;78:21–37.• Pelczar, Michael. Pelczar, Rita- “plant disease”- Encyclopædia Britannica. 2009. Encyclopædia Britannica Online• Singh et al., "Bacillus subtilis as a control agent against fungal pathogens of citrus fruit" Trans. Br. Mycol. Soc.

83(3):487-490 (1984).• Williams & Wilkins- Bergey's Manual of Sytematic Bacteriology, , vol. 2, pp. 1123-1124, 1126, 1133 (1986).• Xin Zhang,1 Bing-xin Zhang,†‡1 Zhen Zhang,2 Wei-feng Shen,2 Ching-hong Yang,3 Jing-quan Yu,4 and Yu-hua Zhao-

Survival of the biocontrol agents Brevibacillus brevis ZJY-1 and Bacillus subtilis ZJY-116 on the spikes of barley in the field- Department of Plant Protection, School of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310029, China

• Yamada et al., "Biological activity of antifungal substances produced by Bacillus subtilis" J. Pesticide Sci. 15(1):95-96 (1990).