Biochemistry 455 Fall Biochemistry 455 Fall 20092009

Techniques in Biochemistry & Techniques in Biochemistry & BiotechnologyBiotechnology

Student comments:“I took a lot out of this class; almost nothing was repetitive of anything I had learned in any other lab.”“I feel that my lab skills improved after taking BIOC 455. I’ve gained more experience and learned a lot of techniques and assays.”“The techniques used here are genuinely applicable, particularly for students already doing research & considering graduate school.”“Things I learned in BIOC 455 will most definitely help me when I enter the workforce.”

Instructor: Gail Grabner (ggrabner@illinois.edu)Topics: Protein purification, Dipeptide sequence analysis, Molecular cloning, ELISA, Lipid analysis

Techniques in Biochemistry & Techniques in Biochemistry & BiotechnologyBiotechnology : BIOC 455

CREDITS: 4 hours

WHEN: M, W Lecture: 1:00-1:50; Lab: 2:00-4:50

WHERE: Lab in 218 Noyes Lab

INSTRUCTOR: Gail K. Grabner

CONTACT: ggrabner@illinois.edu

BIOC 455: Course ContentBIOC 455: Course Content

Dipeptide analysis (2 lab periods) Determination of the amino acid sequence of a dipeptide

Photometry (1 lab period) Colorimetric quantitation of total protein concentration.  

Study of the properties of β-galactosidase (4 lab periods) Kinetic assays of β-galactosidase

Study of carbohydrates (1 lab period) Enzymatic and chemical determination of carbohydrate unknowns

Purification of Glutamate Oxaloacetate Transaminase from pig heart muscle (5 lab periods) Three step purification of an active enzyme from a natural source.  

BIOC 455: Course ContentBIOC 455: Course Content

Study of the interaction of RNA polymerase subunits. (1 lab period) Affinity pull-down of separately expressed RNA polymerase

subunits from Methanococcus jannaschii.  Study of the properties of aspartate transcarbamylase (1

lab period) Kinetic assays of the allosteric enzyme ATCase

Use of the ELISA in immunology (3 lab periods) Determination of the titers of purified sera, unpurified sera, and

monoclonal ascites.  Characterization of Erythrocyte Membranes (2 lab periods)

Extraction of protein and lipid fractions of pig red blood cells.  In vitro transcription/translation (2 lab periods)

Coupled system to produce isotopically labeled protein product

BIOC 455: Student CommentsBIOC 455: Student Comments

BIOC 455: Student CommentsBIOC 455: Student Comments

BIOC 455: Student CommentsBIOC 455: Student Comments