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The U of A 2009 iGEM Project :. BioBytes: A high throughput Method for Minimal Genome construction. What’s what. Synthetic biology - Engineering of organisms to perform artificial functions iGEM - International synthetic biology competition for undergraduate students Team REcoli - PowerPoint PPT Presentation
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BioBytes:BioBytes:A high throughput Method for Minimal A high throughput Method for Minimal
Genome constructionGenome construction
The U of A 2009 iGEM Project:
What’s whatWhat’s whatSynthetic biology - Engineering of organisms to perform artificial functions iGEM - International synthetic biology competition for
undergraduate students Team REcoli- A collection of 30 life sciences, business, computer
sciences, physical sciences and engineering undergraduate students
E. coli- Well studied bacterium naturally found in the
mammalian large intestine.Genome - All of the genes in an organism.
Anderson et al. 2006
Bacteria that target metastatic tumors
New organisms with controlled structure
Inexpensive houses from engineered plants
What could SynBio build?What could SynBio build?
What has SynBio built?What has SynBio built?
Synthetic biological production of the antimalarial drug artemisinin Received $42 million from the Bill and Melinda Gates Foundation http://www.artemisininproject.org
http://openwetware.org/wiki/LightCannon
“Colliroids”
http://2008.igem.org/Team:Harvard
A bacteria powered battery
Project REcoliProject REcoli
What is the minimal set of genes What is the minimal set of genes E.coli needs to survive?E.coli needs to survive?
Objectives:Objectives:1. Propose a minimal genome, about 10% of the original 1. Propose a minimal genome, about 10% of the original
sizesize
2. Develop methods to rapidly assemble hundred of 2. Develop methods to rapidly assemble hundred of genesgenes
3. Develop an automated system for gene assembly3. Develop an automated system for gene assembly
Why minimize a genome?Why minimize a genome?
To understand the requirements for life – if we To understand the requirements for life – if we know what is needed, we can start asking whyknow what is needed, we can start asking why
Develop a simplified, well characterized chasse Develop a simplified, well characterized chasse for other experimentsfor other experiments
To optimize and demonstrate high throughput To optimize and demonstrate high throughput techniquestechniques
How we’re doing itHow we’re doing it
E.coli genomic DNA
Amplify just the essential genes
Add special ends
String genes together on a bead
How we’re doing itHow we’re doing it
Insert into E. coli
Cell division
Move replication origin from original genome to essential genes
Essential genes can replicate, original genome can not
E.coli with Minimal genome
Genomic DNA
Essential genes
ori
ori ori
ori ori
Why it Matters to YouWhy it Matters to You
Interested in a career in biology or engineering?Interested in a career in biology or engineering? Synthetic biology is a promising emerging field.Synthetic biology is a promising emerging field.
Want to do science?Want to do science? High school students and early High school students and early undergraduates can do great research that matters. undergraduates can do great research that matters.
iGEM is just one of many opportunities.iGEM is just one of many opportunities.
Considering the societal impact?Considering the societal impact? Play a role in how Play a role in how our our
society manages synthetic biology through your society manages synthetic biology through your consumer choice, your vote, or your career.consumer choice, your vote, or your career.
EthicsEthics If a man-made genome can sustain a living cell, is If a man-made genome can sustain a living cell, is
that creating life? Should that be permitted? that creating life? Should that be permitted?
Should we be allowed to own an organism whose Should we be allowed to own an organism whose genome we’ve design? genome we’ve design?
Who should be allowed to ‘build their own bug’ Who should be allowed to ‘build their own bug’ with our assembly kit? How can we regulate it?with our assembly kit? How can we regulate it?
Should the genetic parts in the assembly kits be Should the genetic parts in the assembly kits be free, or can the inventor own and charge money free, or can the inventor own and charge money for them? for them?
What do you think?What do you think?