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8/2/2019 Bio Production of Butanol in Bio Reactors
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Luisa Martins
8/2/2019 Bio Production of Butanol in Bio Reactors
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8/2/2019 Bio Production of Butanol in Bio Reactors
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Rubens Maciel Filho
School o Chemical Engineering State University of Campinas
Modeling of Chemical and Biochemical Process: Computer AidedDesign, Computer Aided Process Engineering, Computer Aided
Process Operation Optimization of Chemical and Biochemical Process: Optimization
of Large Scale System, Off-Line Optimization, On-LineOptimization, Real-Time Process Integration.
Advanced Control Algorithms: Advanced Process Control, High
Quality and Performance Process Operation, Soft Sensors, Real-Time Process Integration
8/2/2019 Bio Production of Butanol in Bio Reactors
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Simultaneous acetone butanol ethanol(ABE) fermentation by C. beijerinckii P260
and in situ product recovery
Vacuum process operating in two modes:
Continuous
Intermittent: 1.5 h vacuum with intervals of 4, 6and 8 h
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Controlbatch
fermentation
Incomplete glucose utilization
Butanol Toxicity (13g/L in a batchfermentation)
In Situ
Recovery
Complete glucose utilization Greater productivity
Improved cell growth Concentrated recovered ABE stream
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Vacuum Process Recovery
14.4% to 77.0% of recovery
Presence of fermentation
intermediates Vacuum recovery time
Initial concentration of butanol
Removal rate had to higher
than ABE productivity
2 h to recover 68.5%
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ABE in Batch Bioreactor(Control)
Maximum cell growth at12 h
Residual glucose of 13.9g/L
60 h batch
Produced:
3.7 g/L acetone 11.8 g/L butanol
0.6 g/L ethanol
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ABE by Vacuum Fermentation
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18 h fermentation before vacuum process started
ABE yield is lower
Higher butanol concentration recovered
Continuous: 13.3 g/L 4 h: 35.9 g/L
6 h: 41.4 g/L
8 h: 36.5 g/L
Residual ABE in reactor for intermittent (18%)
C. beijerinckii P260is not adversely affected by thevacuum recovery
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Vacuum technology can be used to recover butanol
Enhanced performance of ABE fermentation due toremovel of butanol
Vacuum fermentation resulted in: Decrease in fermentation time Complete utilization of glucose
Greater cell growth
Concentrated product stream
Intermittent mode is desirable
lower energy costs andhigher ABE concentrations
Best experimental situation: 1.5 h sessions in 4 hintervals 3.7 higher butanol concentration
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Attempt to make biofuel production more attractive
Concerns about productivity
Higher concentrations will lead to less costs in
purification Cost analysis is it worth?
Loss control in vacuum tubing and pumps
Comparison to other used methods (gas stripping)
Using a mutant strain that is more tolerant to butanol C. beijerinckii BA101