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BIO-PROCESS LAB (B) BIO-PROCESS LAB (B) 2010 2010 KAREN LANCOUR KAREN LANCOUR Bio-Process Lab Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR [email protected]

BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR [email protected]

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Page 1: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

BIO-PROCESS LAB BIO-PROCESS LAB (B) (B) 2010 2010

KAREN LANCOURKAREN LANCOUR Bio-Process LabBio-Process Lab

NATIONAL SUPERVISORNATIONAL [email protected]

Page 2: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Bio-Process Lab (B)

Event Description -Event Description - lab-oriented lab-oriented competition involving the competition involving the fundamental science processes of fundamental science processes of a middle school biology lab a middle school biology lab programprogram

Event – Event – lab practical in stationslab practical in stations Event Parameters – be sure to be sure to

check the rules for resources check the rules for resources allowed, type of goggles needed.allowed, type of goggles needed.

Page 3: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

TRAINING MATERIALS

Training HandoutTraining Handout – content Event Supervisor GuideEvent Supervisor Guide – sample

stations, process skills, methods Internet ResourceInternet Resource – links to on-line

courses, lab manuals, notes, sites Sample TournamentsSample Tournaments – sample

stations with key

Page 4: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Basic Science Process Skills

ObservingObserving MeasuringMeasuring InferringInferring ClassifyingClassifying PredictingPredicting CommunicatingCommunicating

Page 5: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Integrated Science Process Skills

Formulating Hypothesis Identifying Variables Defining Variables Operationally Describing Relationships Between

Variables Designing Investigations Experimenting Acquiring Data Analyzing Investigations and Their Data Understanding Cause and Effect

Relationships Formulating Models

Page 6: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Student Preparation

Team work skillsTeam work skills Time limitsTime limits Answering questionsAnswering questions Measurement and CalculationsMeasurement and Calculations Reference materialsReference materials Practice using labs and lab manuelsPractice using labs and lab manuels Construct sample stations Construct sample stations

Page 7: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Compound Microscope

Parts Parts Making wet mountMaking wet mount Appearance of Appearance of

objectsobjects Movement of objectsMovement of objects MagnificationMagnification Changing objectsChanging objects Estimating size of Estimating size of

objectsobjects Field diameter & areaField diameter & area

Page 8: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Principles of Microscopy

Page 9: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Measuring objects

Page 10: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Stereomicroscope PartsParts Appearance of Appearance of

objectsobjects MagnificationMagnification AdvantagesAdvantages UsesUses Observing Observing

objectsobjects

Page 11: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Electronic Balance

CapacityCapacity UnitsUnits Tare or ZeroTare or Zero ErrErr Using the Balance Using the Balance Advantages & Advantages &

Disadvantages Disadvantages

Page 12: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Triple Beam Balance Capacity – auxillary Capacity – auxillary

weights weights Units – numbered Units – numbered

and unnumbered and unnumbered increments increments

Tare Tare Using the Balance Using the Balance Advantages & Advantages &

DisadvantagesDisadvantages

Page 13: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Measuring Liquids

Meniscus – read Meniscus – read bottom bottom

Capacity and Range Capacity and Range Graduations – Graduations –

numbered and numbered and unnumbered unnumbered incrementsincrements

ReadabilityReadability Making measurementsMaking measurements

Estimating Estimating

Page 14: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Metric ruler and calipers Capacity and Range Numbered and

unnumbered increments

cm vs mm Uses of each Making

measurements Estimating Vernier Scale

Page 15: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Thermometers Capacity and RangeCapacity and Range Do not start at zeroDo not start at zero Numbered and Numbered and

unnumbered unnumbered incrementsincrements

Uses of eachUses of each Making Making

measurementsmeasurements EstimatingEstimating

Page 16: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Probes Graphing Graphing

calculatorcalculator Easy link or CBLEasy link or CBL ProbeProbe Collect data onto Collect data onto

calculatorcalculator Transfer data to Transfer data to

computercomputer Graph analysisGraph analysis Quick data Quick data

collectioncollection

Page 17: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Data Presentation and Analysis- Data Tables

Format Format Title Title Units of Units of

measuremenmeasurementt

Numbering Numbering TablesTables

SourceSource

Leg Leg (thigh) (thigh) Length Length (cm)(cm)

Time of 40 Time of 40 yard dash yard dash (sec)(sec)

2424 99

3131 9.29.2

3737 1111

3838 1010

3939 8.28.2

4242 8.48.4

5555 9.39.3

6262 99

Page 18: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Data Presentation and Data Presentation and

Analysis-GraphsAnalysis-Graphs Graph – types Graph – types X vs Y axisX vs Y axis Scaling axisScaling axis Plotting pointsPlotting points Human Error Human Error Curve or best fit Curve or best fit

line line Labeling Labeling

Page 19: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Human Mistakes vs Experimental Errors

Human Mistakes – carelessness Human Mistakes – carelessness Experimental Error – instrument Experimental Error – instrument

variation or technique variation or technique Random Error – chance variationRandom Error – chance variation Systematic Error – system used Systematic Error – system used

for designing or conducting for designing or conducting experiment experiment

Page 20: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

A Sample Dichotomous Key

1. wings covered by an exoskeleton . . . . . . go to step 2  1. wings not covered by an exoskeleton. . . . go to step 3

2 body has a round shape. . . . . . . . ladybug  2 body has an elongated shape. . . . . .grasshopper  

3.wings point out from the side of the body . . dragonfly3 wings point to the posterior of the body.. . . . Housefly

Note: There should be one less step than the total number of organisms to be identified in your dichotomous key.  

Page 21: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Stations – Population Density

Measure sample area Determine population density for symbols Assign an organism to symbols Form a food chain Evaluate sample – predict techniques, etc.

Page 22: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Station – Dichotomous Key

Have specimens of leaves Formulate a key or use a key to identify

specimens

Page 23: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Station - Measurement

Identify range, capacity, incrementation Do measurement – estimate last digit

Page 24: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Station – Experiment Analysis

Analyze the design of the experiment Identify variables Explain results – form conclusions

Page 25: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Station – Microscopy

Determine the diameter of the field and the length of one cell in mm and convert to mcm.

Page 26: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Station – Data Analysis

Making hypothesesMaking hypotheses Food web analysisFood web analysis Eating habit Eating habit

analysisanalysis Predictions and Predictions and

conclusions conclusions InferencesInferences

Page 27: BIO-PROCESS LAB (B) 2010 BIO-PROCESS LAB (B) 2010 KAREN LANCOUR Bio-Process Lab NATIONAL SUPERVISOR NATIONAL SUPERVISOR karenlancour@charter.net

Sample Station – pH

Determine the pH of various solutions using either pH probe or pH test papers.