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Temperature andYeastInvestigationNigel Thomas

Biology13/2/2008

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Data Collection

Table 1 Bubbles !roduced vs" Time at Di#erentTemperatures

$olution

Temperatu

re %&C'

Bubbles !roduced

At 30 seconds At 1 minute At 10 Minutes

( 0 0 0

1( 0 0 0

2( 1 1 30

3( 1) 2* 20+

,( 2 3 3(

(( 0 0 0

*( 0 0 0

)( 0 0 0

Data !rocessing

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-rap. 1 Bubbles !roduced vs" Time at Di#erent

Temperatures

0 1 2 3 4 5 6 7 8 9 100

50

100

150

200

250

5 (!

15 (!

25 (!

35 (!

45 (!

55 (!65 (!

75 (!

Time %inutes'

o" o Bubbles !roduced

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-rap. 2 Bubbles !roduced at 10 minutes vs" at Di#erent

Temperatures

0 10 20 30 40 50 60 70 800

50

100

150

200

250

$olution Temperature %&C'

o" o Bubbles !roduced

Conclusion"t a##ea$s that tem#e$atu$e has a %e$& #$onounced e'ect on the $ate o the

e$mentation $eaction catal&sed )& &east cells* +eing an en,&me- &east cells

ha%e a s#eci.c tem#e$atu$e at /hich the& catal&se most ecientl& and $om the

g$a#h it can )e seen that this o#timum tem#e$atu$e is a##$oimatel& 35*

o/e$ tem#e$atu$es than this do not ha%e enough ene$g& to o%e$come the

$eui$ed acti%ation ene$g& and thus little to no $eaction occu$s* At tem#e$atu$es

highe$ than this- although the acti%ation ene$g& $eui$ement is met- the heat

causes the en,&me to )e denatu$ed and no longe$ act as a catal&st and the

$eaction $ate is once again %e$& lo/*

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valuationAlthough a t$end /as ound- im#$o%ements can )e made to im#$o%e the

accu$ac& o the $esults*

The num)e$ o )u))les /as onl& $eco$ded at 0*5- 1 and 10 minutes and this /as

not sucient to de$i%e a t$end o ho/ the $eaction #$og$essed o%e$ time* The

num)e$ o )u))les should )e taen at e%e$& 30 seconds to gain an

unde$standing o ho/ the $ate o $eaction changes $om sta$t to .nish*

The num)e$ o )u))les /as counted )ut it /as assumed that the %olume o each

)u))le /as unio$m although this ma& not ha%e )een the case* The gas #$oduced

should ha%e )een collected and #$o#e$l& measu$ed o$ %olume as this /ould gi%e

a )ette$ indication o the $ate o $eaction*

"t /as %e$& dicult to maintain the tem#e$atu$e o the solution at the s#eci.edtem#e$atu$e using a the$momete$ and +unsen )u$ne$ alone* A the$mostat

cou#led /ith a hot#late /ould allo/ o$ g$eate$ tem#e$atu$e $egulation and

#$o%ide data that mo$e accu$atel& models the $elationshi# )et/een tem#e$atu$e

and $ate o $eaction*