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Temperature andYeastInvestigationNigel Thomas
Biology13/2/2008
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Data Collection
Table 1 Bubbles !roduced vs" Time at Di#erentTemperatures
$olution
Temperatu
re %&C'
Bubbles !roduced
At 30 seconds At 1 minute At 10 Minutes
( 0 0 0
1( 0 0 0
2( 1 1 30
3( 1) 2* 20+
,( 2 3 3(
(( 0 0 0
*( 0 0 0
)( 0 0 0
Data !rocessing
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-rap. 1 Bubbles !roduced vs" Time at Di#erent
Temperatures
0 1 2 3 4 5 6 7 8 9 100
50
100
150
200
250
5 (!
15 (!
25 (!
35 (!
45 (!
55 (!65 (!
75 (!
Time %inutes'
o" o Bubbles !roduced
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-rap. 2 Bubbles !roduced at 10 minutes vs" at Di#erent
Temperatures
0 10 20 30 40 50 60 70 800
50
100
150
200
250
$olution Temperature %&C'
o" o Bubbles !roduced
Conclusion"t a##ea$s that tem#e$atu$e has a %e$& #$onounced
e'ect on the $ate o the
e$mentation $eaction catal&sed )& &east cells* +eing
an en,&me- &east cells
ha%e a s#eci.c tem#e$atu$e at /hich the& catal&se most
ecientl& and $om the
g$a#h it can )e seen that this o#timum tem#e$atu$e is
a##$oimatel& 35*
o/e$ tem#e$atu$es than this do not ha%e enough ene$g& to
o%e$come the
$eui$ed acti%ation ene$g& and thus little to no $eaction
occu$s* At tem#e$atu$es
highe$ than this- although the acti%ation ene$g& $eui$ement
is met- the heat
causes the en,&me to )e denatu$ed and no longe$ act as a
catal&st and the
$eaction $ate is once again %e$& lo/*
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valuationAlthough a t$end /as ound- im#$o%ements can )e made to
im#$o%e the
accu$ac& o the $esults*
The num)e$ o )u))les /as onl& $eco$ded at 0*5- 1 and 10
minutes and this /as
not sucient to de$i%e a t$end o ho/ the $eaction #$og$essed o%e$
time* The
num)e$ o )u))les should )e taen at e%e$& 30 seconds to gain
an
unde$standing o ho/ the $ate o $eaction changes $om sta$t to
.nish*
The num)e$ o )u))les /as counted )ut it /as assumed that the
%olume o each
)u))le /as unio$m although this ma& not ha%e )een the case*
The gas #$oduced
should ha%e )een collected and #$o#e$l& measu$ed o$ %olume
as this /ould gi%e
a )ette$ indication o the $ate o $eaction*
"t /as %e$& dicult to maintain the tem#e$atu$e o the
solution at the s#eci.edtem#e$atu$e using a the$momete$ and +unsen
)u$ne$ alone* A the$mostat
cou#led /ith a hot#late /ould allo/ o$ g$eate$ tem#e$atu$e
$egulation and
#$o%ide data that mo$e accu$atel& models the $elationshi#
)et/een tem#e$atu$e
and $ate o $eaction*
