Lahore University of Management Sciences
Fundamentals of Molecular Techniques (BIO511) Fall 2013
INSTRUCTORS Drs. Sohail A. Qureshi, Zakir Ullah, Muhammad
Tariq
Room No. Room 10-507 (SAQ); 9-413A (ZU); 9-415A (MT)
Office Hours TBA
Email [email protected]; [email protected];
[email protected]
Telephone X-8349 (SAQ); X-8217 (ZU); X-8218 (MT)
Secretary/TA Haris Manzoor (SAQ); Rana Sarfraz Ilyas (ZU &
MT)
TA Office Hours NA
Course URL (if any) None
COURSE BASICS
Credit Hours Three (3)
Lecture(s) Nbr of Lec(s) Per Week Two (2) Duration 75
minutes
Schedule Tuesdays & Thursdays Time: 11:00 12:15 Venue SDSB
102
Recitation/Lab (per week) Nbr of Lec(s) Per Week NA Duration
Tutorial (per week) Nbr of Lec(s) Per Week NA Duration
COURSE DISTRIBUTION
Core Yes (for graduate students)
Elective Yes (for undergraduates)
Open for Student Category Biology majors and minors
Close for Student Category Others
COURSE DESCRIPTION
Over the past two decades a number of cutting-edge technologies
have been introduced which permit investigators to study cellular
processes and function. As a result there are numerous tools
available to modern day molecular and cell biologists who are
interested in studying genes, proteins as well as cell and
organismal biology. This course has been designed to cover the
modern experimental aspects of molecular and cellular biology.
COURSE PREREQUISITE(S)
Students enrolled in this course are expected to have taken an
introductory level course in molecular biology and a firm grasp of
structure and function of nucleic acids and proteins.
COURSE OBJECTIVES
The objective of this course is to impart theoretical as well as
experimental aspects of various molecular and cell biology
techniques to students with emphasis on how to design experiments
and interpret data.
Lahore University of Management Sciences
Learning Outcomes
After taking this course students should be: Knowledgeable about
methods used for manipulating genes, and analyzing proteins and
nucleic acids Knowledgeable about the various approaches and
instruments used for studying cells Able to understand how
experiments are designed and how their results interpreted Able to
critically evaluate raw experimental data and draw conclusions
Capable of troubleshooting technical problems
Grading Breakup and Policy
Midterm Examination: 40% Project: 20% Final Examination: 40%
Examination Detail
Midterm Exam
Yes/No: Yes Combine Separate: Combined Duration: 3 hours
Preferred Date: None Exam Specifications: Short answer
questions
Final Exam
Yes/No: Yes Combine Separate: Combined Duration: 3 hours Exam
Specifications: Short answer questions
WEEKS TOPICS INSTRUCTOR(S)
Week 1 DNA isolation Electrophoresis Polymerase chain
reaction
MT; SAQ
Week 2 Restriction & Modifying enzymes; DNA Cloning SAQ
Week 3 Recombinant protein production and purification
SAQ
Week 4 Radiolabeling of nucleic acids DNA sequencing Detection
and quantification of RNA
SAQ
Week 5 Analysis of DNA SAQ
Week 6 Analysis of proteins Generation of monoclonal and
polyclonal
antibodies
SAQ
Week 7 Culturing cells and organs Transient and stable
transfections Reporter genes and assays
ZA
Week 8 Stem cells - purification Generation of induced
pluripotent stem cells
ZA
Week 9 Transgenic animals MT
Week 10 Methods of studying gene expression Protein-protein
interactions Protein-DNA interactions
SAQ
Week 11 Studying enzymatic activity in vivo and in
vitro activity Methods for studying signaling pathways
SAQ
Week 12 Methods for studying epigenetics MT
Week 13 Identification of disease causing genes SAQ
Week 14 Recent advances in gene therapy SAQ
