Bio 100 – Guide 07

http://www.nature.com/nbt/journal/v17/n10/images/nbt1099_940.gif

http://www.biologydaily.com/biology/upload/a/ab/Agarose_Gel_Electrophoresis.png

http://www-che.syr.edu/faculty/boddy_group/pages/electrophoresis.jpg

The object of PCR is to make many copies of a specific region of a DNA molecule. All that is needed are short (20-25 bp) primers that uniquely flank the region to be amplified and the enzymes necessary to replicate DNA in a test tube.

http://www.pitt.edu/~heh1/PCR.jpg

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http://www.amebioscience.com/images/pcr- lab.jpg

http://biology.clc.uc.edu/fankhauser/Labs/Genetics/PCR/PCR_images/02_insert_tubes_into_PCR_machine_P2193042crp.JPG

http://www.biochem.arizona.edu/classes/bioc471/pages/Lecture12/mulliBook.jpghttp://www.neue-medizin.com/mullisno.jpg

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Kary Mullis shared the 1993 Nobel Prize in Chemistry

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Borrelia burgdorferithe cause of Lime Disease

http://www.cdc.gov/ncidod/dvbid/lyme/riskmap.htm

Real-Time PCR Detection of Borrelia burgdorferi

RFLP Method - Restriction Fragment Length Polymorphism

http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html

http://homepage.smc.edu/hgp/images/rflp.gif

Paternity Case

Let's use RFLP technology to determine if Jack is the father of Jill's child named Payle. In this scenario, DNA was extracted from white blood cells from all three individuals and subjected to RFLP analysis. The results are shown below:

http://www.bio.davidson.edu/COURSES/genomics/method/RFLP.html

http://www.glofish.com/images/glofish_005_std.jpg

GloFish® fluorescent fish are now on sale at a pet shop near you!

A genetically modified organism (GMO) is an organism whose genetic material has been altered using the genetic engineering techniques generally known as recombinant DNA technology. With recombinant DNA technology DNA molecules from different sources are combined in vitro to one molecule to create a new gene. This modified DNA is then transferred into an organism causing the expression of modified or novel traits. The product is also known as an Genetically Engineered Organism or GEO

GMO Plants are already in the marketplace

•Plants containg the BT-toxin from Bacillus thurngensis that give them natural resistance to insect pests.

•Corn plants containing a gene for resistant to glysophate, a herbicide that makes it easier for fsrmers to kill weeds, but not kill their crop.

•Plants containing a gene from Pseudomonas syringae that makes them frost resistant.

Discussion

The End