BIMM18 Schedule Fall 2019: Sept 2nd, 2019 – Jan 19th, 2020 Course leader: Joan Yuan (Joan.yuan@med.lu.se), Phone: 046-2220430 Module leaders: Cell culturing: Stina Oredsson (Stina.oredsson@biol.lu.se), Phone: 070-3771393 Statistics: Karin Källén (Karin.kallen@med.lu.se), Phone: 046-2227538, Animal models: Madeleine Durbeej-Hjalt (madeleine.durbeej-hjalt@med.lu.se), Phone: 073-6407263, 046-2220812 Immunology: Katharina Lahl (katharina.lahl@med.lu.se), Phone: +45 935 11214

1st week (w36), Cell culturing/Statistics: Introduction to cell culturing

Monday 2 Sept Tuesday 3 Sept Wednesday 4 Sept Thursday 5 Sept Friday 6 Sep

0900-1000

Introduction (SO) BiolD227 1015-1200

Lecture: Cell culturing (SO) Biol D227

1000-1200 Lecture: Cell culturing II (SO) Biol D227

0900-1200 Lecture: Cell culturing III (SO) Biol D227

9.00-12.00 Lecture: Statistics (KK) Room: BMC I1254, BMC H datorsal

13.00-16.00 INTRODUCTION DAY: BIOMEDICAL PROGRAM Segerfalksalen, BMC A-house

1300-1800

Lab: Sterile technique Group A (AMP, MS)

1300-1800

Lab: Sterile technique Group B (AMP, AT) Group II 13.45-15:15 Lecture statistics, (KK) Room I1254 1515-1645 Lab statistics A+C, Computer room BMC H,

1300-1800

Lab: Sterile technique Group C (AMP, AT) Group I 13.45-15:15 Lecture statistics (KK) BMC, Room I1245 1515-1645 Lab, statistics B+D, Computer room

1300-1800

Lab: Sterile technique Group D (SO, AT)

2nd week (w.37) Cell culturing/Statistics: Growth curves, Long-term storage, Routine culture

Monday 9 Sep Tuesday 10 Sep Wednesday 11 Sep Thursday 12 Sep Friday 13 Sep 0900-1200 Lab: Growth curve Routine culture Group I (JS, AMP)

Group I 9-1200 Lab: Growth curve, Group (JS, AT)

Group I 0900-1200 Lab: Growth curve Group I (JS, AT)

0900-1200

Lab: Growth curve, Long-term storage, Routine culture Group I (JS, AT) 1000-1200 Excel exercise, Group II (SO) Room: Biol D226

0900-1200 Lab: Growth curve, Group I (JS, AT) 0930-1200 Base group meeting 1: Group II (SO) Room: Biol B108/B106

1300-1700

Lab: Growth curve, Routine culture Group II (AMP, AT)

Group II 13-1600

Lab: Growth curve, (AMP, MS)

Group II 1300-1600 Lab: Growth curve Group II (AMP, AT)

1300-1600 Lab: Growth curve, Long-term storage, Routine culture Group II (AMP, MS) 1300-1500

Excel exercise Group I (SO) Room: Biol D226

1300-1530 Base group meeting 1: Group I (SO) Room: Biol B106/108 1300-1600 Lab: Growth curve, Group II (AMP, MS)

3rd week (w.38), Cell culturing/Statistics: Routine culturing

Monday 16 Sep Tuesday 17 Sep Wednesday 18 Sep Thursday 19 Sep Friday 20 Sep 0900-1200 Lab: Routine culturing, Long-term storage Group II (AT)

9.00-12.00 Lecture: Statistics (KK) Room: C217 HSC

0930-1200 Lecture: Specific techniques (SO) Room: Biol D227

0900-1200 Lab: Routine culturing, Group II (AT) Group 1 Lecture: Statistics (KK) Room: C217 HSC

0900-1200 Base group meeting 2: Group II (SO) Room: BiolB106/108

1300-1600 Lab: Routine culturing, Long-term storage, Group I (AMP)

1300-1600 Lab: Routine culturing Group I (AMP) Group II Lecture: Statistics (KK) Room: BMC I1345 (Bring own computer!)

1300-1600 Base group meeting 2: Group I (SO) Room: Biol D225/D227

4th week (W.39) Cell Culturing/Statistics: Transfection

Monday 23 Sep Tuesday 24 Sep Wednesday 25 Sep Thursday 26 Sep Friday 27 Sep 0900-1200 Lab: Routine culturing, Wound healing, Group I (SO)

0900-1200 Lab: Wound healing, Group I, Group I (WS)

0800-0915 Practical exam in the cell culturing lab (SO, AMP, AT) Group A 0930-1045

Practical exam in the cell culturing lab (SO, AMP, AT) Group B

1000-1200 Base group meeting 3: Opposition Long-term storage Group I (JS) Room: Biol D225/D227 0900-1200 Lab: Wound healing, Routine culturing, Group II (AT)

1300-1600 Lab: Routine culturing, Wound healing, Group II (SO) Hand in lab report: Long-term storage

1300-1600 Lab: Wound healing, Group II (WS)

1300-1415 Practical exam in the cell culturing lab (SO, AMP, AT) Group C 1430-1545

Practical exam in the cell culturing lab (SO, AMP, AT) Group D

1300-1500 Base group meeting 3: Opposition Long term storage Group II (JS) Room: Biol D225/D227 1300-1600 Lab: Wound healing, Routine culturing, Group I (AT)

13-16 Lecture statistics Room: BMC I1254 (Bring your own computer!) Deadline 13.00: Hand in lab report: Growth curve

5th week (w.40) Cell culturing

Monday 30 Sep Tuesday 1 Oct Wednesday 2 Oct Thursday 3 Oct Friday 4 Oct 0900-10.3000 Lab: Routine culture, Group I (AMP) 10.30-12.00 Lab: Routine culture, Group II (AMP)

9.00-12.00 Statistics repetition (KK) C217

Deadline 13.00: Hand in lab report: Wound healing (very short)

Deadline 13.00: Hand in lab report: Routine culture (very short)

13.00-16.00 Statistics: Written Exam (KK) Room: C217 HSC

6th week (w.41), Cell culturing

Monday 7 Oct Tuesday 8 Oct Wednesday 9 Oct Thursday 10 Oct Friday 11 Oct

9.00-12.00 Written exam Cell culturing Room: Biol D205

1300-1600 Lab follow up: (SO) Room: Biol D205

14-16:00 Novo Nordisk Study Visit Carlsgatan 3 in Malmö (Glasvasen right next to Malmö C). Register prior to 1/10

Schedule Animal Models in Biomedical Research October 14 - November 19, 2019 Course leader: Madeleine Durbeej-Hjalt BMC C12 221 84 Lund Phone: 073-6407263, 046-2220812 Email: madeleine.durbeej-hjalt@med.lu.se Lab tutors: Sara Andersson, Linda Wei and Bodil Sjögreen PBL tutors: Madeleine Durbeej-Hjalt and Kinga Gawlik

Week 42, Oct 14-18, Animal handling and laws Monday 14 Oct Tuesday 15 Oct Wednesday 16 Oct Thursday 17 Oct Friday 18 Oct

9.00-10.00 INTRODUCTION Madeleine Durbeej-Hjalt Lena Uller Room: Segerfalk lecture hall 10.15-12.15 LECTURE Maria Swanberg Biology of the laboratory mouse, rat, hamster, guinea pig and rabbit Room: Segerfalk lecture hall

9.00-12.00 LECTURE Charlotta Grims Post surgical handling Pain perception Anesthesia Analgesia Killing Room: Grubb lecture hall

10.15-12.15 Group A+B PBL 1a Kinga Gawlik Room: Student center H1118 10.15-12.15 Group C+D PBL 1a Madeleine Durbeej-Hjalt Room: Student center H1119

9.00-12.00 INTRODUCTION TO SCIENTIFIC WRITING Matthias Bank Room: BMC H computer room

10.00-12.00 Group A+B DEMONSTRATION/ PRACTICAL Sara Andersson Linda Wei Handling laboratory mice Sex determination Checking vaginal plugs Anatomy Injection techniques Room: D104 Biologihuset (Sölveg. 35)

12.15-13.00 Lunch 12.00-13.00 Lunch 12.00-13.00 Lunch 13.00-15.30 LECTURE Rebecca Sandgren Animal husbandry Health monitoring Aseptic techniques Surgical techniques Sample collection Room: GK lecture hall

13.00-15.00 LECTURE Eva Ekblad Laws concerning animal experimentation Ethical application Room: Grubb lecture hall

13.00-16.00 INTRODUCTION TO SCIENTIFIC WRITING Ladaea Rylander Room: Segerfalk lecture hall

13.00-15.00 Group C+D DEMONSTRATION/ PRACTICAL Sara Andersson Linda Wei Handling laboratory mice Sex determination Checking vaginal plugs Anatomy Injection techniques Room: D104 Biologihuset (Sölveg. 35)

Week 43, Oct 21-25, Animal handling, surgical techniques and reproduction Monday 21 Oct Tuesday 22 Oct Wednesday 23 Oct Thursday 24 Oct Friday 25 Oct

8.00-10.30 RAT LAB Group 1 Bodil Sjögren Biologihuset, room D104 11.00-13.30 RAT LAB Group 2 Bodil Sjögren Biologihuset, room D104

10.15-12.15 LECTURE Bodil Sjögreen Alternatives to animal experiments Room: Seminar room BMC C12

10.15-12.15 Group A+B PBL 2a Kinga Gawlik Room: Student center H1118 10.15-12.15 Group C+D PBL 2a Madeleine Durbeej-Hjalt Room: Student center H1119

PRACTICAL Sara Andersson Linda Wei Surgical technique 09.00-11.00 Group 1 Room: D104 Biologihuset

12.15-13.00 Lunch 12.15-13.00 Lunch 14.00-16.30 RAT LAB Group 3 Bodil Sjögren Biologihuset, room D104

13.00-15.00 Group A+B PBL 1b Kinga Gawlik Room: Student center H1118 13.00-15.00 Group C+D PBL 1b Madeleine Durbeej-Hjalt Room: Student center H1119

13.00-15.00 LECTURE Kinga Gawlik Reproduction in mice Room: Grubb lecture hall

PRACTICAL Sara Andersson Linda Wei Surgical technique 12.00-14.00 Group 2 14.15-16.15 Group 3 Room: D104 Biologihuset

Week 44, Oct 28-Nov 1, Embryology and generation of genetically modified animals Monday 28 Oct Tuesday 29 Oct Wednesday 30 Oct Thursday 31 Oct Friday 1 Nov

10.15-12.15 Group A+B PBL 3a Kinga Gawlik Room: Student center H1118 10.15-12.15 Group C+D PBL 3a Madeleine Durbeej-Hjalt Room: Student center H1119

9.00-11.30 LECTURE Madeleine Durbeej-Hjalt Basic construct design for the generation of different types of transgenic mice and knock-out mice Room: Grubb lecture hall

11.30-13.00 Lunch 13.15-15.15 LECTURE Kinga Gawlik Pre-implantation Implantation Embryonic development Disturbances in development Room: Grubb lecture hall

13.00-15.00 Group A+B PBL 2b Kinga Gawlik Room: Student center I1006 13.00-15.00 Group C+D PBL 2b Madeleine Durbeej-Hjalt Room: Student center I1008

13.00-15.00 LECTURE Thomas Blom ES cell cultivation Room: Segerfalk lecture hall

Week 45, Nov 4-8, Generation of genetically modified animals and geno- and phenotyping Monday 4 Nov Tuesday 5 Nov Wednesday 6 Nov Thursday 7 Nov Friday 8 Nov

09.00-10.30 Group A 10.30-12.00 Group B DEMONSTRATION Sara Andersson Linda Wei Preparation of mouse oocytes Preparation of blastocysts Room: Transgene Lab BMC D10

10.15-12.15 Group A+B PBL 4a Kinga Gawlik Room: Student center H1118 10.15-12.15 Group C+D PBL 4a Madeleine Durbeej-Hjalt Room: Student center H1119

10.15-12.00 LECTURE Madeleine Durbeej-Hjalt Genotype and phenotype analysis of mice and rats Room: Grubb lecture hall

10.00-12.00 Group A+B PRACTICAL: Sara Andersson Linda Wei Age determination of embryonic and fetal stages Room: Transgene Lab D10

13.00-14.30 Group C 14.30-16.00 Group D DEMONSTRATION Sara Andersson Linda Wei Preparation of mouse oocytes Preparation of blastocysts Room: Transgene Lab BMC D10

13.00-15.00 Group A+B PBL 3b Kinga Gawlik Room: Student center H1118 13.00-15.00 Group C+D PBL 3b Madeleine Durbeej-Hjalt Room: Student center H1119

13.00-14.30 Group A 14.30-16.00 Group B DEMO/PRACTICAL Sara Andersson Linda Wei Pronucleus injections Blastocyst injections Room: Transgene lab D10

13.00-14.30 Group C 14.30-16.00 Group D DEMO/PRACTICAL Sara Andersson Linda Wei Pronucleus injections Blastocyst injections Room: Transgene lab D10

13.00-15.00 Group C+D PRACTICAL: Sara Andersson Linda Wei Age determination of embryonic and fetal stages Room: Transgene Lab D10

Week 46, Nov 11-15, Presentation of ethical application and literature task Monday 11 Nov Tuesday 12 Nov Wednesday 13 Nov Thursday 14 Nov Friday 15 Nov

10.15-12.15 PRESENTATION OF HOME WORK WITH ETHICAL APPLICATIONS Anders Forslid and Madeleine Durbeej-Hjalt Groups: special presentation list will be given Room: I1245/1250

10.15-12.15 PRESENTATION OF HOME WORK WITH ETHICAL APPLICATIONS Anders Forslid and Madeleine Durbeej-Hjalt Groups: special presentation list will be given Room: I1245/1250

10.15-12.15 PRESENTATION OF HOME WORK WITH ETHICAL APPLICATIONS Anders Forslid and Madeleine Durbeej-Hjalt Groups: special presentation list will be given Room: I1245/1250

11.30-13.30 PRESENTATION OF LITERATURE TASKS Madeleine Durbeej-Hjalt Anja Meissner Animal models in the study of human diseases Room: Segerfalk lecture hall

14.00-16.00 LECTURE Madeleine Durbeej-Hjalt Generation of monoclonal and polyclonal antibodies Room: Grubb lecture hall

13.00-15.00 Group A+B PBL 4b Kinga Gawlik Room: Student center H1118 13.00-15.00 Group C+D PBL 4b Madeleine Durbeej-Hjalt Room: Student center H1119

13.00-18.00 PRESENTATION OF LITERATURE TASKS Madeleine Durbeej-Hjalt Anja Meissner Animal models in the study of human diseases Room: Grubb lecture hall

14.00-17.00 PRESENTATION OF LITERATURE TASKS Madeleine Durbeej-Hjalt Anja Meissner Animal models in the study of human diseases Room: Segerfalk lecture hall

Week 47, Nov 18-22, Examination, Introduction to Immunology and T cell lectures Monday 18 Nov Tuesday 19 Nov Wednesday 20 Nov Thursday 21 Nov Friday 22 Nov

12.30-16.30

EXAMINATION (written test) Room: Grubb lecture hall

11.15-13.00 LECTURE INTRODUCTION TO THE IMMUNE SYSTEM Katharina Lahl Room: BMC I 1245, 1250

10.15-12.00 LECTURE T CELLS PART 1 Karin Leandersson Room: BMC I 1245, 1250

10.15-12.00 LECTURE T cells Part 2 Karin Leandersson Room: Rune Grubb-Salen

Week 48, Nov 25-29, T cell group work and B cell lectures Monday 25 Nov Tuesday 26 Nov Wednesday 27 Nov Thursday 28 Nov Friday 29 Nov

10.15-12.00 Case group work T CELLS (Thomas Bäckström) Room: BMC I 1108, 1109, 1251, 1253

10.15-12.00 LECTURE B CELLS PART 1 Joan Yuan Room: BMC I 1245, 1250

10.15-12.00 LECTURE B CELLS PART 2 Joan Yuan Room: BMC I 1245, 1250

11.00-12.00 FACS LECTURE Lab 1 Katharina Lahl Room:

12.15-13.00 Lunch 12.15-13.00 Lunch 13.15-15.00 iRAT/tRAT T CELLS Thomas Bäckström Room: HSC B215

13.15-15.00 Case group work T CELLS (Thomas Bäckström) Room: BMC I 1208, 1211, 1251, 1253

13.00-15.00 CASE SUMMARY T CELLS Thomas Bäckström Karin Leandersson Room: HSC C315

13.00-15.00 FACS LAB Lab 1 Katharina Lahl + Tutors Room: BMC D14

Week 49, Dec 2 - 6, B cell group work and Mucosal Immunology lectures

Monday 2 Dec Tuesday 3 Dec Wednesday 4 Dec Thursday 5 Dec Friday 6 Dec

10.15-12.00 Case group work B CELLS (Thomas Bäckström) Room: BMC I 1108, 1109, 1251, 1253

10.15-12.00 LECTURE MUCOSAL IMMUNOLOGY PART 1 Katharina Lahl Room: BMC I 1245, 1250

10.00-12.00 FACS LAB Lab 2 Groups A+B Katharina Lahl + Tutors Room: BMC D14

11.30-13.00 Lunch 13.15-15.00 iRAT/tRAT B CELLS Thomas Bäckström Room: HSC C315

13.15-15.00 Case group work B CELLS (Thomas Bäckström) Room: BMC I 1208, 1211, 1251, 1253

13.00-15.00 CASE SUMMARY B CELLS Thomas Bäckström Joan Yuan Room: HSC B215

13.15-15.00 LECTURE MUCOSAL IMMUNOLOGY PART 2 William Agace Room: Segerfalksalen

13.00-15.00 FACS LAB Lab 2 Groups C+D Katharina Lahl + Tutors Room: BMC D14

Week 50, Dec 9-13, Mucosal Immunology group work and Autoimmunity Lectures Monday 9 Dec Tuesday 10 Dec Wednesday 11 Dec Thursday 12 Dec Friday 13 Dec

10.15-12.00 Case group work MUCOSAL IMMUNOLOGY (Thomas Bäckström) Room: BMC I 1108, 1109, 1251, 1253

10.15-12.00 LECTURE AUTOIMMUNITY AND TOLERANCE PART 1 Anja Schmidt-Christensen Room: BMC I 1245, 1250

10.15-12.00 LECTURE AUTOIMMUNITY AND TOLERANCE PART 2 Anja Schmidt-Christensen Room: BMC I 1245, 1250

10.00-12.00 FACS LAB Lab 3 Groups C+D Katharina Lahl + Tutors Room: BMC D14

13.15-15.00 iRAT/tRAT MUCOSAL IMMUNOLOGY Thomas Bäckström Room: HSC C315

13.15-15.00 Case group work MUCOSAL IMMUNOLOGY (Thomas Bäckström) Room: BMC I 1208, 1211, 1251, 1253

13.00-15.00 CASE SUMMARY MUCOSAL IMMUNOLOGY Thomas Bäckström Katharina Lahl Room: HSC B235

13.00-15.00 FACS LAB Lab 3 Groups A+B Katharina Lahl + Tutors Room: BMC D14

Week 51, Dec 16-20, Autoimmunity and Tolerance group work Monday 16 Dec Tuesday 17 Dec Wednesday 18 Dec Thursday 19 Dec Friday 20 Dec

10.15-12.00 Case group work AUTOIMMUNITY AND TOLERANCE (Thomas Bäckström) Room: BMC I 1108, 1109, 1251, 1253

10.00-12.00 FACS LAB Lab 3 Groups A+B Katharina Lahl + Tutors Room: BMC D14

12.00-13.00 Lunch 13.15-15.00 iRAT/tRAT AUTOIMMUNITY AND TOLERANCE Thomas Bäckström Room: HSC C315

13.15-15.00 Case group work AUTOIMMUNITY AND TOLERANCE (Thomas Bäckström) Room: BMC I 1208, 1211, 1251, 1253

13.00-15.00 CASE SUMMARY AUTOIMMUNITY AND TOLERANCE Thomas Bäckström Anja Schmidt-Christensen Room: HSC C315

13.00-15.00 FACS LAB Lab 3 Groups C+D Katharina Lahl + Tutors Room: BMC D14

Week 2, Jan 6-10, Applied Immunology Lectures and Group Work Monday 6 Jan Tuesday 7 Jan Wednesday 8 Jan Thursday 9 Jan Friday 10 Jan

10.15-12.00 LECTURE APPLIED IMMUNOLOGY PART 1 Bengt Johansson-Lindbom Room: BMC I 1245, 1250

10.15-12.00 LECTURE AUTOIMMUNITY AND TOLERANCE PART 1 Karin Leandersson Room: BMC I 1245, 1250

10.15-12.00 iRAT/tRAT APPLIED IMMUNOLOGY Thomas Bäckström Room: HSC C315

10.15-12.00 Case group work APPLIED IMMUNOLOGY (Thomas Bäckström) Room: BMC I 1208, 1211, 1251, 1253

13.15-15.00 FACS LAB SUMMARY Katharina Lahl Room: BMC I 1251, 1253

13.15-15.00 Case group work APPLIED IMMUNOLOGY (Thomas Bäckström) Room: BMC I 1208, 1211, 1251, 1253

Week 3, Jan 13-17, Applied Immunology Summary and Examination

Monday 13 Jan Tuesday 14 Jan Wednesday 15 Jan Thursday 16 Jan Friday 17 Jan

09.00-13.00 EXAM Katharina Lahl Room: Fernströmsalen

13.00-15.00 CASE SUMMARY APPLIED IMMUNOLOGY Thomas Bäckström Karin Leandersson Room: HSC C315