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AMT AND ITS METABOLITES 251
than NT level. In the urine, AMT, NT, 10-OH-AMT, 10-OH-NT are excreted in the range of 0-1, 1-2, 2-6 and 10-50 m g / g c rea t in ine respectively. The rat io of 1 0 - O H - N T / N T in the ur ine ranged 10-30 for pa- t ients with normal liver funct ion. Our observat ions about correlat ion between serum and u r i n a r y levels of AMT and metaboli tes and control of depression will be published elsewhere.
ACKNOWLEDGEMENTS
For f inanc ia l support to the Medical Research Council, G ran t No. MA-5563 and St. Joseph's Hospital Foundat ion, Gran t No. 820-042.
For advice and research samples to Alber t Manian, N.I.M.H. and Merck Research Ins t i tu te .
For help in the collection and handl ing of blood sam- pies and collection of pa t ient data to Lois Barry.
For secretar ia l assis tance to Anne Robitaille.
REFERENCES
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(1973). Clh~. Toxicol. 6:571-584. ]3. Usdin, E. and Efron, D. H. (1972). Psychotropic
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LETTER TO THE EDITOR
Bilirubin Quantitation with Lipemic Plasma
Clin. Biochem. 9, 2, 96-98 (1976)
It has been brought to our attention that while the AO bilirubinometer was rather insensitive to Intralipid lipe- mia in bilirubin estimation in artificially jaundiced hu- man serum albumin, this principle does not necessarily apply to jaundiced plasma. The effects of lipemia on bili- rubin estimation by the AO bilirubinometer were therefore determined in four different adult plasma samples (Tri- glyceride El-G) less than 20 mg/dl, Bilirubin less than 0.5 mg/dl) . As with Human Serum Albumin, TG levels of 50 mg / /d l or less caused no interference in bilirubin estimations. At higher concentrations of Intralipid (100- 750 mg/dl) , there is a linear increase of l mg/dl of bili- rubin per 100 mg/dl (see figure l) and this effect seems to be independent of the bilirubin concentration used in the experiments (2.5 to 25 mg/dl) . Therefore, if the TG concentrations in the samples are known a subtraction of 1 mg/dl bilirubin per 100 mg/dl of lipid will give the actual bilirul~in concentration.
George Chan, Kathy Merrills and David Schiff, Department of Pediatrics
University of Alberta Edmonton, Alberta.