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Bigelo
RT
ow Lab
Research The Gulf
REU
borato
Experienf of Main
U SymposiumThursday
1
ory for
nce for Une and the
m Program y, August 9,
Ocean
Undergrae World
& Abstract, 2012
n Scien
aduates Ocean
ts
nces
2
Program 1:00 Opening Remarks 1:15 Marie Neidig- University of Southern Maine
PRODUCTION AND ASSIMILATION OF DIMETHYLSULFONIONPROPIONATE (DMSP) BY MARINE PHYTOPLANKTON (Matrai – Archer)
1:30 James (Nick) Hunt- Texas A&M University – Corpus Christi.
ENHANCEMENT OF LIPID PRODUCTION IN MICROALGAE BY DODECANE ADDITION AND NITRATE LIMITATION (Wilson- Countway)
1:45 Cynthia Liles- University of California, Riverside
THE RELATIONSHIP BETWEEN MICROALGAE GROWTH PHASE AND LIPID CONTENT (Countway-Wison)
2:00 Emily Bell-Hoerth- Earlham College
VARIATIONS IN PHYTOPLANKTON COMMUNITIES ASSOCIATED WITH BIVALVE AQUACULTURE IN COASTAL MAINE (Poulton - Heil)
2:15 Zakary Jaques- Colby College
EFFECTS OF DISSOLVED HUMIC COMPOUNDS FROM THE DAMARISCOTTA AND KENNEBEC RIVERS ON MARINE MICROBIAL COMMUNITIES. (Poulton - Heil)
2:30 Andrew Burchill – University of Chicago
DETECTING EVOLUTIONARY SELECTION IN HIGHLY CONSERVED PROTEINS: PHYSIOCHEMICAL SHIFTS IN THE MAMMALIAN CYTOCHROME B (McClellan)
2:45 Break (15 minutes) 3:00 Ashley Poehls – Lake Superior State University
EFFECTS OF OCEAN ACIDIFICATION ON GROWTH RATE, BIOMASS, AND COCCOLITH MORPHOLOGY OF THE COCCOLITHOPHORE PLEUROCHRYSIS (Balch - Fields)
3:15 Melanie Ross - Colby College
INDIRECT EFFECTS OF OCEAN ACIDIFICATION ON ACARTIA TONSA (Balch - Fields)
3:30 Alyson Lowell - University of Maine
EXPERIMENTAL TECHNIQUES FOR CHARACTERIZING SHEATH-FORMING ZETAPROTEOBACTERIA: A METHODOLOGICAL APPROACH (Field-Emerson)
3:45 Kaitlyn Pritchard – Northeastern University
EXAMINING THE GRAZING PREFERENCES AND DIGESTIVE PROCESSES OF OXYRRHIS MARINA USING A RATIOMETRIC FLUORESCENT DYE (Neuster-Twinning)
3
Abstracts
PROMAR Mari1Uni DimantiosulfupartiDunatertiorespeno adhuxleDMSloweabunDMS
ODUCTIONRINE PHY
ie Neidig1,2, iversity of So
methylsulfonioxidant and/our for bacteriiculate DMSaliella tertioolecta produectively. Totdjustment toeyi. T. pseudSPd did not est in bacterindance and aSPd levels.
N AND ASSTOPLANK
Dr. Patriciaouthern Mai
opropionateor cryoproteial organism
SP(p) by threolecta and a uced no DMStal DMSPp c
o intracelluladonana enhachange as a ized treatmenactivity in th
IMILATIOKTON
a Matrai2 & Dine, 2 Bigelow
(DMSP) is ectant. DMSP
ms. We measuee axenic phycoccolithophSPp, while Tconcentratioar DMSP witanced its DMfunction of nts, especiale saline cond
ON OF DIM
Dr. Steve Arw Laborator
produced byP, when releured the proytoplankton hore EmilianT. pseudonanons for all spth cell growt
MSPp producsalinity, aftelly at 50 g/kgdition, despi
4
METHYLSU
rcher2 ry for Ocean
y marine phyeased into seduction and species (a d
nia huxleyi) na and E. huecies increasth. DMSPd wction under ler the initial g salinity. Thite decreased
ULFONION
n Sciences
ytoplankton eawater, is an
assimilationdiatom Thala
and one bacuxleyi had losed as a funcwas assimilalow salinity cosmotic shohis is likely d phytoplank
NPROPIONA
worldwide an important n of dissolveassiosira tertcterized straiow and high ction of cell ated only by conditions w
ock. DMSPddue to the hikton abundan
ATE (DMS
as an osmolysource of ca
ed DMSP(d)tiolecta, a chin of T. pseuDMSPp probiovolume dT. pseudona
whereas the rd concentratiigher bacterince and unch
P) BY
yte, arbon and and hlorophyte
udonana. D. oduction, displaying ana and E. release of ions were ial hanged
ENHNIT
AlgaphotThisPavlLipidwerecultucytomfactoan inbetwprod
HANCEMERATE LIM
1T
ae, a renewabtosynthetic o research foclova lutheri cd concentrate divided intures. Samplemetry. The mor 15 for the ncrease by th
ween days 6 aduction, and
ENT OF LIPMITATION
J. NTexas A&M
ble fuel sourorganisms prcused on enhcultures withtions were mo two treatm
es were stainmain findingP. lutheri do
he average faand 13 compare a solid fo
PID PRODU
Nicholas HuUniversity –
rce, can poteroduce lipidshancing lipidh the hydroc
monitored usiments: dodecned with a 1:gs display a lodecane treaactor 2.52 anpared to the foundation fo
UCTION IN
unt1, William– Corpus Ch
entially replas that can be d productioncarbon dodecing the fluorane-adminis100 dilutionlipid productatments betwnd maximumcontrols. Str
or further str
5
N MICROA
m H. Wilson2
risti, 2Bigelo
ace significanconverted in
n by stressingcane and deprescent dye, Bstered culturen BODIPY, ation increase
ween days 6 m factor 5.25ressing cells ress-induced
ALGAE BY
2 and Peter Cow Laborato
ant fossil fuento renewabg Dunaliellapleting nitratBODIPY 50es during staand analyzede by the averand 13, after
5 for D. tertiovia these mlipid enhanc
DODECAN
Countway2 ory for Ocean
l consumptioble fuel for da tertiolecta,te amounts in05/515, and fationary phad for green frage factor 5r dodecane wolecta nitratethods resulcement inve
NE ADDITI
n Sciences
on levels. Thdiesel engine
Isochrysis gn the growthflow cytome
ase and nitratfluorescence 5.22 and maxwas adminise-limited trelt in increaseestigations.
ION AND
hese s: biodiesel.galbana, andh medium. etry. Strains te-limited by flow ximum tered, and
eatments ed lipid
d
THE CynWill Therand rThe undenoveand fthrouwhenprodclaimphas
E RELATION
thia Liles, Eie Wilson, N
re is growingrenewable enlipids produ
erstand how el dye used tflow cytomeugh exponenn placed into
duction in somm. Isolation ase with no sig
NSHIP BET
EnvironmenNational Cen
g concern fonergy source
uced by algaegrowth relato detect alga
etry offers quntial to statioo new mediame algal straand treatmengnificant cha
TWEEN MIC
ntal Sciencenter for Mari
r climate che. Algae utile have been tes to lipid cal lipids by fualitative anonary phase. a to mimic laains, howevents of each gange in grow
CROALGAE
es, Universitine Algae an
ange as the Ulize energy fridentified as
content to hafluorescencealysis of lipiPavlova lut
ate lag phaseer, the additigrowth phasewth.
6
E GROWTH
ty of Californd Microbiot
United Statefrom the sun s a potential rvest algae a
e. This dye inid productivtheri has beee. It is reportion of glucose showed an
H PHASE AN
rnia, Riversta, Bigelow L
es struggles tand carbon source of bi
at optimal con combinatio
vity throughoen determineted that orgase or glycero
n increase in
ND LIPID C
side; Dr. PetLaboratory f
to find a cosdioxide, a m
iodiesel. It isonditions. BOon with epiflout the algal ed to have thanic carbon sol did not inclipid conten
CONTENT
te Countwayfor Ocean Sc
st-efficient, smajor greenhs therefore imODIPY 505/luorescence growth cycl
he highest lipsources boosclusively sup
nt only durin
y and Dr. ciences
sustainable, house gas. mportant to /515 is a microscopy le, from lag pid content st lipid pport this g late lag
VARBIV EmiEarlh BivasurroDiarundethe eedulDamsampchardiatopositcouldamou
RIATIONVALVE AQ
ily Bell-Hoeham College
alve aquacultounding watrrhetic Shellferstand the reeffect of nutris and a bio-
mariscotta Ripled on a regacterized usioms. Detritaltively to nutrd prove harmunts.
NS IN PHYQUACUL
rth1, Cynthie1, Bigelow L
ture provideer. Harmful fish Poisoninelationship brient addition-fouling macver and incugular basis. Cing an imagil matter incrrient stimulamful to huma
YTOPLANLTURE IN
ia Heil2 and Laboratory f
s substrate falgal specie
ng (DSP), habetween cultns on the microalgal specubated in situChanges in ting-flow-cytreased on botation within an health an
NKTON CN COASTA
Nicole Poulfor Ocean Sc
for epibenthies, such as Prave been asstured bivalveicroalgal comcies, Ulva lau with and wthe phytoplantometer (Floth Ulva and the first 24 hd bivalve far
7
COMMUNAL MAIN
ton2
ciences2
ic microalgarorocentrumociated withes and their ammunities astuca. Ostrea
without nutrienkton comm
owCAM). Aloyster incubhours on botrms. Proroc
NITIES ASNE
ae and can alm lima, whichh bivalve aquassociated mssociated wia edulis and ents (Osmoc
munity on eacll communitibations over th substrates
centrum lima
SSOCIAT
lter nutrient dh produces ouaculture in
macro and mith the EuropU. latuca we
cote©) in Wech substrate ies were domtime. Pseud
s. This potena was not pre
TED WITH
dynamics inokadaic acidthe Gulf of M
microalgae, wpean Oyster,ere collectedest Boothbayover time w
minated by pdonitzschia rntially toxic sesent in sign
H
n the d causing Maine. To
we examined Ostrea d from the y Harbor and
were pennate responded species
nificant
d
EF
GloborgaCDOuptakLakeEast fractRivedecrdiffeexamthe Ghigh
FFECTS OF
bal warming anic matter (COM, have beke, carbon ae on microbiBoothbay H
tionated chloer DHS additeases in cert
erent sourcesmined (bacteGulf of Mainher trophic le
F DISSOLVER
1
and changinCDOM) into
een shown toassimilation aial communiHarbor, and corophyll a. Ations within tain phytoplas documenteeria, phytoplane may potenevels.
ED HUMICRIVERS ON
Zakary J
Colby Colle
ng precipitato the Gulf ofo influence mand growth. ity compositcharacterizedAn initial 12the first 24 h
ankton groupd rapid (6 hrankton, or hentially signif
COMPOUNN MARINE M
Jaques1,2, Cy
ege, 2Bigelow
tion patternsf Maine. Dismicrobial gro
The effects ion was examd using flow
20 hr experimhours; with ips. A secondr) responses eterotrophs) ficantly impa
8
NDS FROMMICROBIA
ynthia Heil2,
w Laboratory
have increassolved humowth throughof DHS isolmined using
w cytometry, ment documeincreases in d experimentto additionsand DHS so
act microbia
M THE DAMAL COMMU
Nicole Pou
y for Ocean
ased the transmic substanceh trace metallated from th
g in situ incuimaging cyt
ented the comtotal chloropt examining s depending ource. Resulal community
MARISCOTTUNITIES.
lton2
Sciences
sport of rivees (DHS), thl chelation, ehe Kennebec
ubations of setometry (Flommunity resphyll and bathe effect ofupon comm
lts indicate cy compositio
TA AND KE
erine coloredhe major comeffects uponc River or Deawater fromowCAM), ansponse to Keacteria, as wef DHS addit
munity compochanging DHon, in turn e
ENNEBEC
d dissolved mponent of n nutrient amariscotta
m West and nd size-ennebec ell as tions from onent
HS levels in ffecting
DETPHY And1Uni Cytoelectlongadap(Woanalyusingaminphysfor rproteto ad
TECTING EYSIOCHEM
drew Burchiiversity of C
ochrome b (ctron transpor
gevity, obesitptations. Conolley et al., 2ytical methog the physicno acid chansicochemicalobust interprein. This studaptation dur
EVOLUTIOMICAL SHI
ill1, David Mhicago, 2Big
cytb) is a conrt chains invty, and age-rnsequently, t2003), I ana
od has demonochemical p
nges and the l shifts in funretations of tdy suggests ring mamma
ONARY SELIFTS IN TH
McClellan2 gelow Labor
nserved protvolved in resprelated diseathere are fewlyzed cytb senstrated the
properties assproperties innctional protthese resultsthat several
alian evoluti
LECTION HE MAMMA
ratory for Oc
ein found inpiration and
ases like Alzhw mammal-wequences frosensitivity nsociated withnvolved in thtein regions s in terms of regions of thon.
9
IN HIGHLALIAN CY
cean Science
n nearly all euphotosynthe
heimer's. Mawide studies com 28 specienecessary to h structure ahe adaptive palso were de
f the electronhe protein th
LY CONSERYTOCHROM
es
ukaryotes anesis. Some hany statisticacharacterizines from nearcharacterizeand functionprocess wereescribed. Th
n transport anhought to be
RVED PROME B
nd prokaryothuman cytb mal models fang cytb evolrly all major e the evolution. The locatioe identified. he well-studind proton pu nonfunction
OTEINS:
tes, and is comutations afail to detect slution. Usingmammal lin
on of conseron of importDirectional
ied nature ofumping funcnal have bee
ommon in ffect subtle g TreeSAAPneages. This rved proteinstant cytb
f cytb allowsctions of the en important
P
s
s
INDMela1Colb Atm97%unit Intergthencoccwere(280AddperiForaninfollo
IRECT EFFanie Ross1, Dby College, 2B
mospheric pC% by the end
decrease in governmentaneriticcalancolithophoreeallraised0ppm,380pitionally,deodsthrough280ppm,thncreasegrazowedby280
FECTS OF ODavid FieldsBigelow Labo
CO2 has risenof the centurthe oceans, pl Panel on Clnoidcopepoe,PleurochryatapCO2leppm,and75e‐platedPlehoutthe48hegrazingrazingrates.T0ppmand3
OCEAN ACIs2, Barney Boratory for Oc
n by 36% overy. The oceapredicted to imate ChangeodAcartiatorysis,weremvelof380p0ppm,reprurochrysis‐hourre‐plaatesdecreasThehighest380ppmresp
IDIFICATIOalch2, Steve cean Sciences
er the preindanic absorptidecline anot
e. Thisstudyonsa.Ingestmeasuredinpmwhilethresentingprfromallthratingprocessedduringtingestionrpectively.
10
ON ON ACAShema2, As
s, 3Lake Supe
dustrial levelion of that inther 0.3 to 0yinvestigattionratesofn24‐hourbohealgaewere‐industriareepCO2levsstocompathere‐platinatesforreg
ARTIA TONshley Poehlserior State Un
l and is expenflux of atmo0.46 pH unitstedindirectfA.tonsafeottleexperiregrownatal,current,avelswerefeareingestionngprocess,gularlyplate
NSA 3
niversity
ected to increospheric COs by 2100 acteffectsofoedingonthimentsat16tthreedistiandpredictedinthreesnrateswithwhile380aedcellswer
ease by an adO2 has causedccording to thoceanacidifiecoastal6ºC.ThecopnctpCO2levedCO2inpueparate4‐hhcells’litheand750ppmrefrom750p
dditional d a 0.1 pH he icationon
pepodsvelsts).hourgrazingstructure.mbothsawppm,
g
EXPZETAlys
1TheEast Zetapiron formwhatsheasequsigna
PERIMENTTAPROTEOson Lowell1,
e University Boothbay H
proteobacteis oxidized,
m is largely ut is a largely
ath-forming Zuences to genatures. 375 1
TAL TECHNOBACTERIA
2, Dr. Erin F
of Maine, OHarbor, Main
eria (“Zetas”extracellula
unknown, hoy inorganic mZetaproteobanerate fluore16S rRNA Z
NIQUES FOA: A METHField2, Dr. D
Orono Mainene 04544
”) are marinear structures owever studiematrix of Fe-acteria. Inve
escent in situZetaproteoba
OR CHARAHODOLOGIDavid Emerso
e, 04469; 2Bi
e microorgansuch as helices have indic-oxides. Thisestigations c
u hybridizatioacteria gene
11
ACTERIZINICAL APPRon2 and Dr. D
igelow Labo
nisms that ocal stalks or cated that pos research focentered on ion (FISH) prsequences w
NG SHEATROACH David McCl
oratory for O
xidize iron itubular shea
olysaccharidocused on preidentifying crobes and to
were compilesourccompUnfoalignworkreproCompdetersheatorganprecibactepolysSpectranspsheatfreshtargepolys
TH-FORMI
lellan2
Ocean Scienc
in microaeroaths form. H
des are an orgeliminary at
characteristico identify noved from bothces representpilation of Zeortunately, dinment were ok must be donoducibility anparative gen
rmine possibth-forming "nisms represipitates and neria were usesaccharide exifically, a larporter genesth-forming B
hwater iron oeted as potensaccharide ex
ING
ces, 60 Bigel
ophilic envirHow these strganic compottempts to idc “Zeta” shevel genes ash public and ting the larg
Zetaproteobaiscrepanciesobserved andne to increasnd robustnes
nomics was uble genetic si"Zetas.” Eighsenting stalknon-structured to determxport genes.rge number s were identiBetaproteobaoxidizer. Fivntial genes inxport.
low Drive,
onments. Asructures onent of entify
eath-forming genetic in-house est cteria. in sequence
d additional se ss. used to ignatures forht novel s, sheaths, e forming
mine . of ABC ified in acteria, a e genes were
nvolved in
s
g
e
r
e
Abstan adunitsthis sPleuconc
EFFECTS
tract: Atmosdditional 100s and IPCC pstudy we exa
urochrysis spcentrations o
S OF OCEANMORPH
Ash1 Lake Su
spheric pCO2
0% by the enpredictions samined the e
p. Growth ratof 280, 380, a
N ACIDIFICHOLOGY O
hley Poehls1
uperior State
2 levels havend of the censuggest an adeffects of incte, culture biand 750 ppm
CATION ONOF THE COC
, David FielUniversity,
e increased bntury. Risingdditional deccreased pCOiomass, and
m. SEM, bire
12
N GROWTHCCOLITHO
lds2, Barney 2 Bigelow L
by 36% over g pCO2 has acrease of 0.3
O2 levels on tcell biomas
efringence, a
H RATE, BIPHORE PLE
Balch2, StevLaboratory fo
r the last centalready caus3 to 0.45 pH the calcifyins were meas
and light micvisstruincandandcelimastruresphyaltecocconproproeco
OMASS, ANEUROCHRY
ven Shema2
or Ocean Sc
tury and aresed ocean pH
units by theng coccolithosured at calibcroscope imasualize pCO2
ucture. Datacreasing pCOd culture biod X respectivll biomass inages revealeucture with isults indicateysiologicallyered by oceaccolithophorntributors tooductivity, coperties, thesological imp
ND COCCOYSIS
iences
expected toH to decreasee end of the cophore algaebrated pCO2
ages were us2 effects on ca shows that O2 levels, groomass decreavely, while i
ncreased by Xed a compromincreasing pe that Pleuroy and morphan acidificatires are major the ocean’sarbon cycle,se effects ha
plications.
OLITH
o increase bye by 0.1 century. In e, sed to cell and lith with owth rate ased by X individual X. SEM mised lith
pCO2. These ochrysis are hologically ion. Becauser
s primary , and optical
ave many
y
e
EXAMAR Pritc Nues Twin Moszoopinfluand bvacuphytfluor10 tidigeinvercoccquan
AMINING TRINA USIN
chard, K. R.,
ster, J., Bige
ning, B. S., B
t of the iron plankton grazuences of prebioavailabili
uoles of the htoplankton prescent dye times more ofst these cellsrsely propor
colithophoresntitatively de
THE GRAZNG A RATI
Northeaster
elow Laborat
Bigelow Lab
used by phyzers. Undersey-type on thity of regeneheterotrophicrey (diatom,to compare df a chlorophs. However, rtional to pres than with determine the
ZING PREFIOMETRIC
rn University
tory for Oce
boratory for
ytoplankton standing chehis processinerated iron. Ac dinoflagell, coccolithopdigestive vacyte than a coinitial fluore
ey preferencedinoflagellateffect of pre
FERENCESC FLUORES
y, Boston, M
ean Sciences
Ocean Scien
in high-nutremical proceng is thereforAs such, we late Oxyrrhisphore, chlorocuole acidityoccolithophoescence ratioe, with vacuotes. Additioney digestion
13
S AND DIGESCENT DY
MA, USA, ka
, East Booth
nces, East Bo
ient, low-chssing of ironre useful to ustudied the gs marina. Wophyte) overy with each pore (the leastos (with highole fluoresce
nal work willon iron spec
ESTIVE PRYE
aitlynpritcha
hbay, ME, U
oothbay, ME
hlorophyll aren within grazunderstandingrazing pref
We comparedr 24 hours. Inprey. Overalt consumed ph ratios correence ratios al constrain pciation.
ROCESSES
ard1@gmail.
USA, jnuester
E, USA, btw
eas comes frzer digestiveng grazer coferences andd grazing raten addition, wll, we found prey) and toesponding toapproximatelpH from thes
S OF OXYR
.com
wining@bige
rom reminere systems anntrols on the
d digestion ofes of three tywe used a ratthat Oxyrrh
ook 2-3 timeso low pH) wly 6 times hise ratios and
RRHIS
org
elow.org
ralization byd the e speciation f prey withinypes of tiometric is consumeds as long to
were igher with
d
n
d
ThaFouNSFSiteOceResMai
anks to the Nundation for F Grant OCEe: Bigelow Lean Sciencessearch Experine and the W
14
National Scieits support uE 1156740 –
Laboratory fos – Undergrarience in the World Ocean
ence under – REU or aduate
Gulf of n.