BioNordikaBergman AS • tel 23 03 58 00 • fax 23 03 58 01 • info@bioberg.no • www.bionordikabergman.no

October - December 2015

Issue 4bergman

Contents

Our new website . . . . . . . . . . . . . . . . . page 2

Water purification . . . . . . . . . . . . . . . . page 3

Rotavapor R-300 . . . . . . . . . . . . . . . . . . page 4

Lab reactor . . . . . . . . . . . . . . . . . . . . . . page 5

Centrifuge . . . . . . . . . . . . . . . . . . . . . . . page 5

DNA/RNA UV-cleaner box . . . . . . . . page 5

DNA/RNA analysis . . . . . . . . . . . . . . . page 6

DNA purification kits . . . . . . . . . . . . . page 6

DNA extraction kit . . . . . . . . . . . . . . . . . page 6

NanoDrop One . . . . . . . . . . . . . . . . . . page 7

Media preparation . . . . . . . . . . . . . . . . page 7

transOMIC . . . . . . . . . . . . . . . . . . . . . . page 8

New products from NEB . . . . . . . . . page 8

FlashGel . . . . . . . . . . . . . . . . . . . . . . . page 10

New antibodies from CST . . . . . . . page 10

Primary cells . . . . . . . . . . . . . . . . . . . page 11

Antibody production . . . . . . . . . . . . page 12

Cell death kit . . . . . . . . . . . . . . . . . . . . . page 12

Cytation 5 . . . . . . . . . . . . . . . . . . . . . . . page 13

Plate washers and readers. . . . . . . page 15

Our service team . . . . . . . . . . . . . . . page 16

2

NEW WEBSITE!www.bionordikabergman.no

BioNordikaBergman is proud to finally introduce to you our new webpage. With this page, we provide you with information about our consumables, research products and instruments, all one site.

What is new?

• Webshop! You may set up a new user account and thus get access to prices and order entry. And if you need primer or probes our oligonucleotide order form is still available.

• Product search! Search for specific products and get easy access to all the alternatives that we offer from our different suppliers.

• Easy access to special offers, news and activities!

As a distributor of several suppliers we offer a huge product portfolio, and our new website will only give you a small overview of what we can offer. For more product details, you can follow links directly to our suppliers. If you can’t find your favorite product, please do not hesitate to contact us by e-mail or phone.

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Do you want to learn more about our products for Water Purification?Contact Monica Laukas at 404 40 960

monica@bioberg.no

WATer purIFICATIoN

PurELaB® flex 3 - The most intuitive and innovative system for all your pure water needs The pureLAB flex is uniquely designed around your needs. It combines ease of use with the accurate delivery of pure water. The flexible modular system can be configured to deliver the quality of water you need for your applications.

Features• Intuitive to use, ergonomic handset design • Clear water purity display for absolute confidence as you dispense • real-time ToC monitoring for critical analytical applications • prioritized information shown at all times (system status, ToC, alarm) • point of use filters for multiple applications• Flexible dispensing by: - Variable flow - drop by drop or up to 2 liters per minute - AutoVolume dispense with 50ml to 60 liters and repeat dispensing - Hands free with optional foot pedal - Locked dispense for glassware filling

Specifications Flex 3• Daily volume: <10 liters• Dimensions: (W) 236mm (D) 470mm (H) 900mm (max. height: 102 cm)• operational Weight: 23 kg• Feedwater pressure (max): 90psi (6 bar) (If <60psi (4 bar) a separate boost pump is required)• Mains Input: 100-240V ac, 50-60Hz

PURELAB ChoRUs - In Tune with your NeedsThe pureLAB Chorus gives you the chance to configure a solution that best suits your needs with the added benefit of flexibility should your future needs change. Ideal for a wide range of applications including sophisticated research and testing applications, qualitative analyses, feeding ultrapure water systems, glassware washing, autoclaves etc. The list of possible applications is endless!

Featuring:• PURELAB Chorus 1: ultrapure water for critical applications• PURELAB Chorus 2: for your pure Type II+/II solutions• PURELAB Chorus 3: for your general grade Type III solutions• Halo Dispense Solutions: three remote dispensing and monitoring solutions• Storage Reservoirs: designed to accomodate PURELAB Chorus water purification systems

4

Quality in your handswww.buchi.com/rotavapor

Rotavapor® R-300More convenient. More effective. More for you.

∙ Control all process parameters with a central interface

∙ Automate your individual SOPs using configurable methods

∙ Distill even foaming samples without supervision

∙ Receive push notifications on your mobile device when a process is finished

Do you want to learn more about Rotavapors?Contact Marte Tøften at 922 11 099

marte@bioberg.no

The r-300 meets the highest expectations in convenience and versatility. Its modular design allows extension of the rotavapor® R-300 to a fully integrated system where a central interface regulates each component.

Your most important benefits:

Convenient• Comfortable flask and vapor duct removal thanks to easy to use Combi-Clip• user-friendly operation due to ergonomic electric or manual lift• Immediate adjustment of heating temperature and rotation speed with intuitively positioned control knobs

Extendable and versatile• easy plug & play extension to a fully integrated system including a central interface, vacuum pump and recirculating chiller according to your needs• Choice between 5 L high-temperature and 1 L fast-heating bath• Wide range of beneficial accessories available: seven different glass assemblies, safety shield, lid for heating bath, automatic distillation and automatic foam detection

reproducible• precise operation control with integrated digital display of heating temperature, rotation speed and lift position• Traceability of all process parameters including data recording and charting with the optional Interface I-300 pro• Instant alert with push notification on mobile devices in case of process deviation (with Interface I-300 / I-300 pro only)

Introduction offer: Prices from Kr. 32 000,-

NEW!

Do you want to learn more about Lab Reactors?Contact Marte Tøften at 922 11 099

marte@bioberg.no

5

THe reACTor-reADY™ LAB reACTor

reactor-ready is designed as a universal reactor work station with a range of easily interchangeable vessels from 100ml to 5 litres which can be configured to suit the chemistry and scale needed for each project. easy to use, this one unique framework can re-place many, saving money and fumehood space.

LAB eQuIpMeNT FroM BIoSAN

NEW! Centrifuge/Vortex for PCR plates CVP-2 (cat.no. BS-010219-A02) is a fully automatic device for reproducing The Spin–Mix–Spin technology for 2 pCr plates at the same time, thus saving time considerably. The Spin–Mix–Spin technology is intended to spin-down micro volumes of reagents on the well’s bottom (first centrifugation spin), following mixing (mix) and spin-down the reagents again from the walls and cap of the well (second spin). This repetitive algorithm of operation is aimed to reduce the mistakes during sample preparation for pCr analysis.

A must-have instrument for PCR and DNA analyses laboratory.Includes rotor for 2 pCr plates, adapters for semiskirted and unskirted pCr-plates.

DNA/RNA UV-cleaner box uV-cleaner boxes are equipped with an open uV lamp installed in the upper hood. uV-radiation from the open lamps disinfects the working area inactivating DNA/rNA fragments during 15–30 min of exposure. A digital timer controls duration of the direct uV irradiation. A daylight lamp provides proper illumination of the working surface.uV-cleaner boxes are equipped with a flow-type bactericidal uV cleaner- recirculator Ar, which provides constant decontamination inside the box during operation. They are recommended for operations with DNA/RNA amplicons.

NOW! We offer 20% discount on all DNA/RNA UV Cleaner Boxes from Biosan!

-20 %

Do you want to learn more?Contact Kristin Lundemo at 992 27 101

kristinl@bioberg.no

Reactor Features• rapid, tool-free vessel exchange with quick-release vessel clamp and hose couplings.• Accepts all leading brands of overhead stirrer and allows easy, tool-free adjustment.• Triple support stand features heavy duty stainless steel support rods for stability.• Self-aligning stirrer coupling engages without the need for tools.• Innovative hose manifolds allow easy thermofluid drain down.• Temperature range: -70ºC to +230ºC.• Wide range of accessory glassware including condensers, dropping funnels etc.• optional software allows you to log and control stirrers, circulators, balances, pumps, temperature sensors and other devices.

reaction Vessel Features• Range of single and vacuum jacketed vessels from 100ml to 5 litres.• Process vessels to mimic larger scale plant or manufacturing reactors.• Precision engineered borosilicate glass vessels.• DN100 5 neck Glass Lid fits all Vessel sizes.• Vessels feature V4 Zero Dead Space Drain Stopcock which prevents over-tightening which can cause glass breakage.

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Ladder of successThe Shimadzu concept of DNA/RNA analysis is faster and more sensitive than ever before. Providing an inexpensive alternative to agarose gel electrophoresis, the Shimadzu MultiNA system is the ladder of success to a new level of electrophoresis analysis simplifying the daily routine, e.g. in forensics and clinical diagnostics.

• High sensitivity detection

• High resolution and reproducibility

• Fully-automated fast analysis

• Direct documentation of results

EurogENtEc NEWS!

SMarTPurE The Smartpure Kits are easy-to-use kits intended for quick and efficient minipurification of DNA fragments and plasmid DNA. They contain a Smart-Pure column made of a silica based membrane which separates efficiently the DNA molecules from the reaction mixture.With the Smartpure kits the isolated DNA can be used immediately in all your routine molecular biology applications.

The Smartpure plasmid Kit provides high quality purified plasmid DNA. The plasmid isolated from bacterial cell culture can be used directly in any downstream molecular biology applications. Working time: +/- 20 minutes. Typical yield: Up to 40 μg plasmid DNA / 1-5 mL E.coli culture**Depending on the plasmid copy number and the culture OD.

The Smartpure pCr Kit It is perfectly suited to efficiently separate DNA fragments from pCr or enzymatic reaction mixtures without using toxic phenol and chloroform solvants. Working time: +/- 6 minutes. Typical yield: From 60 bp to 10 kbp. Elution recovery: ≥99%

The Smartpure Gel Kit A convenient kit for the extraction and purification of DNA fragments from up to 3% standard (or high/low melting) agarose gel in either TAe or TBe buffer.Working time: +/- 16 minutes. Typical yield: From 60 bp to 23 kbpElution recovery: ≥99%

SMArTpure BeNeFITS

• Easy to use: Each kit is provided with a Smart Protocol card

• Fast: purification completed in a few minutes

• efficient: High recovery yield

• Convenient: 100 Preps pack size and stable for 18 months

Cat. no. Product INTroDuCTIoN prICeSK-pLpu-100 Smartpure plasmid kit, 100 preps 539,-SK-pCpu-100 Smartpure pCr kit, 100 preps 539,-SK-Gepu-100 Smartpure Gel kit, 100 preps 539,-

Do you have questions about DNA extraction and purification?Contact Gitika at 940 07 207gitika@bioberg.no

SMarTEXTraCT The Smartextract DNA extraction kit achieves genomic DNA extraction from almost any crude sample type (e.g. blood, human & animal tissues, plant seeds & leaves...). DNA extraction is performed in a single tube in 12 minutes only. The recovered solution can be used directly in any amplification reaction (pCr or end point qpCr).

SMArTFLeX BeNeFITS

• Easy to use: extraction in 1 tube only, with no precipitation, no column

• Fast: extraction completed in 12 minutes

• efficient: Ideal for rapid genotyping

• Universal: A single kit for various sample types

• Convenient: Stable at room temperature, and compatible with robotics

Cat. no. Product INTro. PRICE

SK-DNeX-100 SmartExtract DNA kit , 100 preps

887,-

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Do you want to learn more about NanoDrop?Contact Kristin Lundemo at 992 27 101

kristinl@bioberg.no

NotE!Information regarding NanoDrop 1000All service and support on NanoDrop 1000 will end in December 2015. unfortunately there will be no more new software releases or all hardware support.

Do you need aquipment for media preparation?

We offer petri dish fillers and media preparators from Integra, and can advice you to find the best solution for your laboratory!

Do you want to learn more?Contact Monica Laukas at 404 40 960monica@bioberg.no

NEW! MoVe oNe STep CLoSer To SuCCeSS WITH NANoDrop oNe

Quantify and qualify DNA, rNA, and protein samples with only 1–2 μL in seconds with no dilutions using the Thermo Scientific™ NanoDrop™ one microvolume uV-Vis spectrophotometers. Gain a more complete understanding of sample quality before using samples in downstream applications with Thermo Scientific™ Acclaro™ Sample Intelligence technology built into every NanoDrop One instrument.

• Fast and easy evaluation of nucleic acids and proteins with Auto- Measure and pre-programmed applications• Modern standalone design with local control and high-resolution touchscreen interface saves bench space• Accurate measurements up to 27,500 ng/μL (dsDNA) with extended auto-range pathlength technology• No consumables needed – pipette directly onto the pedestal sample-retention system• enhanced connectivity with data transfer via uSB, ethernet, Bluetooth® and Wi-Fi options; pC software available for data management• optional cuvette position for measuring dilute solutions and performing temperature sensitive experiments

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NEB NEWS! rapid PNGase F pNGase F is the most effective enzymatic method for removing N-linked oligosaccharides from glyco- proteins. pNGase F digestion deaminates the asparagine residue to aspartic acid, and leaves the oligosaccharide intact for further analysis. Rapid PNGase F is an improved reagent that allows the complete and rapid deglycosylation of antibodies and immunoglobulin fusion proteins, as well as other glycoproteins, in minutes. All N-glycans are released rapidly and without bias, and are ready to be prepared for downstream chromotography or mass spectrometry analysis.

other Glycobiology Reagents Now Availableα1-2,4,6 Fucosidase is a highly specific exoglyco- sidase that catalyzes the hydrolysis of α1-2, α1-4 and α1-6 linked l-fucopyranosyl residues from oligo- saccharides. α1-3,4,6 Galactosidase is a highly specific exoglyco- sidase that catalyzes the hydrolysis of α1-3, α1-4 and α1-6 linked d-galactopyranosyl residues from oligo-saccharides.

New Restriction EnzymesThe new restriction enzymes from NeB are all active in the CutSmart® Buffer.

Visit www.neb.com to view the full list of restriction enzymes available from NeB.

Cat. no. ProductP0710S Rapid PNGase F

Cat. no. ProductP0748S/L α1-2,4,6 FucosidaseP0747S/L α1-3,4,6 Galactosidase

Cat. no. ProductR3575S/L BsrGI-HF®R0680S/L BssSαIR0667S/L BtsαIr3198S/L MluI-HFr3192S/L NruI-HFR3127S/L NsiI-HF

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Do you have questions about products from TransoMIC and NEB?

Contact Gitika Emberland at 940 07 207gitika@bioberg.no

and more NEB NEWS!....

NEB® Golden Gate Assembly MixThe efficient and seamless assembly of DNA fragments, com- monly referred to as Golden Gate assembly, has its origins in 1996 when, for the first time, it was shown that multiple inserts could be assembled into a vector backbone using only the sequential or simultaneous activities of a single type IIS restriction enzyme and T4 DNA Ligase. This method can be accomplished using Type IIS restriction enzymes, such as BsaI, and can also be used for the cloning of single inserts. The method is efficient and can be completed in one tube in as little as 5 minutes for single inserts, or can utilize cycling steps for multiple inserts. Golden Gate Assembly has been widely used in the construction of custom-specific TALeNs for in vivo gene editing, among other applications.

Cat. no. ProductP0710S Rapid PNGase F

Cat. no. ProductP0748S/L α1-2,4,6 FucosidaseP0747S/L α1-3,4,6 Galactosidase

In its simplest form, Golden Gate Assembly requires a Type IIS recognition site, in this case, BsaI (GGTCTC), added to both ends of a dsDNA fragment. After digestion, these sites are left behind, with each fragment bearing the designed 4-base overhangs that direct the assembly.

Cat. no. ProductE1600S NEB Golden Gate Assembly Mix

hiscribe™ T7 ARCA mRNA KitsMost eukaryotic mrNAs require a 7-methyl guanosine (m7G) cap structure at the 5 ́ end and a poly(A) tail at the 3 ́end to be efficiently translated. The HiScribe T7 ArCA mrNA Synthesis Kit (NeB #e2060) is designed to synthesize capped and tailed mrNAs for variety of applications. Capped mrNAs are synthesized by co-transcriptional incorporation of Anti-reverse Cap Analog (ArCA) using T7 rNA polymerase. A poly(A) tail is then added by e. coli poly(A) polymerase. A separate version of the kit, without e. coli poly(A) polymerase (NeB #e2065), is available for use with DNA templates encoding a poly(A) stretch or not requiring a poly(A) tail. The kits also include DNase I and LiCl for DNA template removal and quick mrNA purification.

Both cap and tail are required for mrNA function in cell culture Luciferase expression in U2OS cells. Purified Cypridina luciferase RNA produced as indicated was co-transfected into U2OS cells with purified Gaussia luciferase mRNA. mRNAs produced using the HiScribe T7 ARCA mRNA Kit (with Tailing) are 5 ́-capped and have 3 ́ poly(A) tails. After 16 hours incubation at 37°C, cell culture supernatants from each well were assayed for CLuc and GLuc activity. Luminescence values were recorded and used to calculate relative luciferase activity.

Cat. no. ProductE2060S HiScribe T7 ArCA mrNA Kit (with tailing)E2065S Hiscribe T7 ArCA mrNA Kit

NEBNext Library Quant Kit for Illumina®Accurate quantitation of next-generation sequencing libraries is essential for maximizing data output and quality from each sequencing run. Furthermore, for Illumina sequencing, accurate quantitation of libraries is critical to achieve optimal cluster densities, a requirement for optimal sequence output. qpCr is considered to be the most accurate and effective method of library quantitation, providing considerably higher consistency and reproducibility of quantitation. Amplification-based methods quantitate only those molecules that contain both adaptor sequences, thereby providing a more accurate estimate of the concentration of the library molecules that can be sequenced. The NeBNext Library Quant Kit delivers significant improvements to qpCr-based library quantitation for next gen sequencing.

Cat. no. ProductE7630S/L NeBNext Library Quant Kit for Illumina

Place an order of any 3 or more products from Cell Signaling Technology using the promotion code eu15SLS, and receive a 4th product free.*

*promotion code eu15SLS must be referenced when placing order. offer applies to purchases of 3 catalog items or more and excludes peptide (40 ul) or smaller sized products. Free item must be of equal or lesser value to the lowest priced item.

FLASHGeL DeVICe pACK - GEL ELECTrOPhOrESIS aNd GEL dOCuMENTaTION IN ONE uNITComplete separation, recovery and documentation safely, at the bench, in minutes.

With the FlashGel™ System, you get great resolution and fast results. Simply load samples, watch bands migrate and get data in as little as 2 minutes. Say goodbye to gel preparation, band excision, purification, and uV light.

experience the Speed of the FlashGel™ System• 5 minute separation and recovery• excellent band resolution• precast, prestained agarose gels and buffer - no need for gel preparation, buffer addition or gel staining

The FlashGel™ Device pack is the easy way to speed up your DNA and rNAseparation and recovery. All the equipment you need, in one part number.

10

STeM CeLL AND DeVeoLpMeNT TArGeTS

Do you want to learn more?Contact Kristin Lundemo at 992 27 101

kristinl@bioberg.no

Cat. no. Product Special offer57062 FlashGel™ Device pack

(Incl. FlashGel™ Dock, FlashGel™ power Supply and FlashGel™ Camera)

16 500,-Do you have questions about FlashGel?

Contact Gitika Emberland at 940 07 207gitika@bioberg.no

oct-4A (D6C8T) Rabbit mAb (mouse specific) #83932Marker of stem cell pluripotency. WB, Ip, IF, ChIp and Flow Cytometry validated.

Confocal IF using #83932 (green) and phalloidin #13054 (red).

New sox Family Rabbit mAbs for IhC and IFSox 9 rabbit mAb #82630WB, IHC and IF validated.

Sox10 rabbit mAb #89356WB, Ip and IF validated.

IHC analysis using #82630.

PRoX1 (DsJ6J) Rabbit mAb #14963Transcription factor that plays critical roles in organ development as well as eMT in certain cancers.WB and IHC validated.

IHC analysis using #14963.

BUY 3 PRODUCTS AND GET A 4TH FREE

15FLYCORPPRDI0016ENG_02

For Research Use Only. Not For Use In Diagnostic Procedures. © 2015 Cell Signaling Technology, Inc. Cell Signaling Technology and CST are trademarks of Cell Signaling Technology, Inc.

YOUR CST DiSTRiBUTOR:

Place an order with Cell Signaling Technology (CST) for 3 or more products using the promotion code EU15SLS, and receive a 4th product free.*

BUY 3 GET 4th FREE!

Use Code EU15SLS When Placing Order

If you would like to know more about this promotion or our product listings, please contact your local distributor.

*Offer only valid in AT, CH, CZ, DE, DK, ES, FN, FR, IT, NO, PL, PT, SE, TR. Expires December 31st, 2015. No cash or cash equivalent. No substitutions. Offer valid on new orders only. Offer may not be applied to existing, pending or prior orders. Promotion code EU15SLS must be referenced when placing order. Offer applies to purchases of 3 catalog items or more and excludes petite (40µl) or smaller sized products. Free item must be of equal or lesser value to the lowest priced purchased item. Cannot be combined with any other promotions. Limit 1 promotion per order. Items that are not eligible, tax and shipping and handling charges will not be included in determining merchandise subtotal.

BioNordikaBergman AS Gladengveien 3B, 0661 Oslo Tel: 23 03 58 00 Faks: 23 03 58 01E-post: info@bioberg.no

11

BioResearch

Primary Cells ‘Anniversary’ Ad – BioTechniques

Clonetics™ Primary Cells and Media The Trusted Path to Relevant Results

www.lonza.com/primary© 2014 Lonza Walkersville, Inc

Choose Lonza for Optimal Cell PerformanceLonza’s Clonetics™ Primary Cells and Media are globally renowned for quality and reliability. As the pioneers of primary cell culture, Lonza celebrates 30 years of providing these products and expertise to scientists for consistent and biologically relevant results every time.

Our breadth of cell-type offering is unmatched and spans across research areas such as respiratory, neurobiology, cancer, stem cells, diabetes, cardiovascular and others.

Clonetics™ Primary Cells Portfolio: – 100+ cell types – cryopreserved or proliferating, human and animal – Broad donor variety – large selection of donors for most cell types – Cells from normal and diseased tissues – Optimized Growth Media BulletKit™ for each cell type

The trusted path to results starts with Lonza.

Clonetics™ Primary Cells

30th

Anniversary

PrimaryCells_Ad_BioTech.indd 1 5/7/14 11:52 AM

STeM CeLL AND DeVeoLpMeNT TArGeTS

12

reference SPEEdY PTM

Antigen = peptide produced by Eurogentec

1 modified (pTM)1 unmodified

peptide design Includedpeptide synthesis >70%

10-13 aa15-20 mg (modified)

10-20 mg (unmodified)peptide coupling to carrier Coupling of 5 mg peptide

Carrier KLH, BSA, oVA or THY28-Day Program Yes

Rabbits 2Elisa Guarantee optional

Affinity purification + eLISA Double purification of 50 ml seriumELISA

Aditional services optional Shipment on dry ice Yes

Delivery purified AntibodyRemaining Serum

remaining peptide

SpeeDY 28-DAY Your Polyclonals in 28 days only!

Speedy 28-Day is the reference program for the production of custom polyclonal antibodies at eurogentec. This proprietary program uses a non-Freund adjuvant that stimulates the immune response and gives mostly IgG’s, despite the short immunization time.

speedy Post Translational ModificationSpeedy 28-Day includes a very efficient immunization protocol with frequent boosts. It is therefore well adapted to generate Abs, specifically targeting pTMs, as it can minimize instability of the modification(s) harbored on the peptide used as antigen.

By far the most popular requested pTMs specific Abs are directed to phosphorylated residues but we can also do Anti-Methylation, Anti-Acetylation and many more pTM Abs.

Do you want to learn more about Custom Antibody Production?Contact Målfrid Røe at 975 90 405malfrid@bioberg.no

BackgroundpArp1 is the primary member of the poly(ADp-ribose) poly-merase family, whose function is to signal DNA damage (and to recruit repair proteins) by pArylation. pArp1 is also involved in multiple cell death pathways, including apoptosis, necroptosis, autophagy, and a relatively new pathway termed parthanatos. It has been implicated in a new form of cell death termed parthanatos. pArp1 can also promote tissue survival by shifting the balance of cell death programs between autophagy and necrosis. Clinical studies have shown vulnerability to PARP inhibitors in DNA repair defective cancers. Anti-pArp1 (internal) antibody is useful for researchers interested in cellular processes including DNA damage, transcriptional control, and stem cell identity research.

Application NoteAnti-pArp1 (internal) antibody with the matched secondary has been validated by western blotting and nanoimmunoassay (NIA). Specific conditions for reactivity should be optimized by the end user. expect a band approximately 113 kDa in size corresponding to pArp-1 by western blotting in the appropriate cell lysate or extract.

PARP1 (internal) Antibody Combo Pack Properties

Cat. no. K-X51 Host Rabbit Known Cross reactivity HumanClonality Polyclonal Application Western Blot : 1:500 - 1:2000Kit Type Combo Pack

HIGHLY SpeCIFIC AND SeNSITIVe NANoIMMuNoASSAY oF pArp1a toolkit to measure cell death

Questions?Contact Kristin Lundemo at 992 27 101

kristinl@bioberg.no

13

Do you want to learn more about Cytation™ 5?Contact hanne Ramstad at 414 79 591

hanne@bioberg.no

Cytation™ 5 is a uniquely integrated, configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate detection to provide phenotypic cellular information and well-based quantitative data. This instrument replaces multiple modules and software interfaces, yet is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular visualization in fluorescence, brightfield, H&E stain and phase contrast channels. The multi-mode detection module features BioTek’s patented Hybrid Technology™, which incorporates variable bandwidth

monochromator optics and high sensitivity filter-based detection optics for unmatched versatility and performance. Temperature control to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors optimize conditions for cell-based imaging and detection. Image capture, data collection and powerful image and data analysis are managed with Gen5™ software, specifically designed for uncomplicated processing of even the most complex assays.

Features:

• Fluorescence, brightfield, H&E stain and phase contrast microscopy and conventional multi-mode detection

• Patented Hybrid Technology™ in multi-mode detection combines high performance filters with variable bandwidth monochromators for versatility and performance

• Variable bandwidth fluorescence monochromators offer a 9 nm to 50 nm in 1 nm increments for ultimate flexibility

• Laser-based excitation for AlphaScreen® / AlphaLISA® assays

• CO2/O2 control, incubation to 65 0C and shaking optimize cell- based and other assays

• Low volume (2 µL) nucleic acid and protein quantification with the available Take3 Plates

• Powerful yet easy to use Gen5 software for efficient plate reading, image capture and analysis

www.biotek.com

Euglena gracilis, 60x TiliaStem, 2x Z-stack, 20xZebrafish, 4x

Cytation 5 with dual injector (left) and CO2/O2 Gas Controller (right)

Scan with your smart phone to watch the video.

Cytation™ 5 is a uniquely integrated, configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate detection to provide phenotypic cellular information and well-based quantitative data. This instrument replaces multiple modules and software interfaces, yet is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular visualization in fluorescence, brightfield, H&E stain and phase contrast channels. The multi-mode detection module features BioTek’s patented Hybrid Technology™, which incorporates variable bandwidth

monochromator optics and high sensitivity filter-based detection optics for unmatched versatility and performance. Temperature control to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors optimize conditions for cell-based imaging and detection. Image capture, data collection and powerful image and data analysis are managed with Gen5™ software, specifically designed for uncomplicated processing of even the most complex assays.

Features:

• Fluorescence, brightfield, H&E stain and phase contrast microscopy and conventional multi-mode detection

• Patented Hybrid Technology™ in multi-mode detection combines high performance filters with variable bandwidth monochromators for versatility and performance

• Variable bandwidth fluorescence monochromators offer a 9 nm to 50 nm in 1 nm increments for ultimate flexibility

• Laser-based excitation for AlphaScreen® / AlphaLISA® assays

• CO2/O2 control, incubation to 65 0C and shaking optimize cell- based and other assays

• Low volume (2 µL) nucleic acid and protein quantification with the available Take3 Plates

• Powerful yet easy to use Gen5 software for efficient plate reading, image capture and analysis

www.biotek.com

Euglena gracilis, 60x TiliaStem, 2x Z-stack, 20xZebrafish, 4x

Cytation 5 with dual injector (left) and CO2/O2 Gas Controller (right)

Scan with your smart phone to watch the video.

Cytation™ 5 is a uniquely integrated, configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate detection to provide phenotypic cellular information and well-based quantitative data. This instrument replaces multiple modules and software interfaces, yet is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular visualization in fluorescence, brightfield, H&E stain and phase contrast channels. The multi-mode detection module features BioTek’s patented Hybrid Technology™, which incorporates variable bandwidth

monochromator optics and high sensitivity filter-based detection optics for unmatched versatility and performance. Temperature control to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors optimize conditions for cell-based imaging and detection. Image capture, data collection and powerful image and data analysis are managed with Gen5™ software, specifically designed for uncomplicated processing of even the most complex assays.

Features:

• Fluorescence, brightfield, H&E stain and phase contrast microscopy and conventional multi-mode detection

• Patented Hybrid Technology™ in multi-mode detection combines high performance filters with variable bandwidth monochromators for versatility and performance

• Variable bandwidth fluorescence monochromators offer a 9 nm to 50 nm in 1 nm increments for ultimate flexibility

• Laser-based excitation for AlphaScreen® / AlphaLISA® assays

• CO2/O2 control, incubation to 65 0C and shaking optimize cell- based and other assays

• Low volume (2 µL) nucleic acid and protein quantification with the available Take3 Plates

• Powerful yet easy to use Gen5 software for efficient plate reading, image capture and analysis

www.biotek.com

Euglena gracilis, 60x TiliaStem, 2x Z-stack, 20xZebrafish, 4x

Cytation 5 with dual injector (left) and CO2/O2 Gas Controller (right)

Scan with your smart phone to watch the video.

Cytation™ 5 is a uniquely integrated, configurable system that combines automated digital widefield microscopy with conventional multi-mode microplate detection to provide phenotypic cellular information and well-based quantitative data. This instrument replaces multiple modules and software interfaces, yet is simple to setup and operate. With up to 60x magnification, the microscopy module provides high-quality cellular and sub-cellular visualization in fluorescence, brightfield, H&E stain and phase contrast channels. The multi-mode detection module features BioTek’s patented Hybrid Technology™, which incorporates variable bandwidth

monochromator optics and high sensitivity filter-based detection optics for unmatched versatility and performance. Temperature control to 65 °C and shaking, plus available CO2/O2 control and dual reagent injectors optimize conditions for cell-based imaging and detection. Image capture, data collection and powerful image and data analysis are managed with Gen5™ software, specifically designed for uncomplicated processing of even the most complex assays.

Features:

• Fluorescence, brightfield, H&E stain and phase contrast microscopy and conventional multi-mode detection

• Patented Hybrid Technology™ in multi-mode detection combines high performance filters with variable bandwidth monochromators for versatility and performance

• Variable bandwidth fluorescence monochromators offer a 9 nm to 50 nm in 1 nm increments for ultimate flexibility

• Laser-based excitation for AlphaScreen® / AlphaLISA® assays

• CO2/O2 control, incubation to 65 0C and shaking optimize cell- based and other assays

• Low volume (2 µL) nucleic acid and protein quantification with the available Take3 Plates

• Powerful yet easy to use Gen5 software for efficient plate reading, image capture and analysis

www.biotek.com

Euglena gracilis, 60x TiliaStem, 2x Z-stack, 20xZebrafish, 4x

Cytation 5 with dual injector (left) and CO2/O2 Gas Controller (right)

Scan with your smart phone to watch the video.PARP1 (internal) Antibody Combo Pack Properties

Cat. no. K-X51 Host Rabbit Known Cross reactivity HumanClonality Polyclonal Application Western Blot : 1:500 - 1:2000Kit Type Combo Pack

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Do you want to learn more?Contact hanne Ramstad at 414 79 591

hanne@bioberg.no

ELx50 strip washer

• Washes 96- and 384-well microplates• Magnetic and polystyrene bead assays• ELISAs and cell-based assays• Filtration-to-waste processes• patented Dual-Action™ wash manifold for independent control of dispense and aspiration tubes• Comprehensive onboard software makes programming quick and easy• Automatic switching for up to three wash buffers• programmable shaking duration and intensity• Built-in maintenance programs

Epoch & Take3 nanodrop plateA microplate reader and a nanodrop in one instrument

• Save valuable sample by using Take3 to quantitate 2 μL volumes of DNA, rNA and proteins• Monitor cell growth using the Epoch’s well scanning capability• run spectral scans of 2 μL samples, or samples contained in a microplate or cuvette• Dial in individual wavelengths from 200 – 999 nm• perform rapid qualitative or quantitative analysis in microplates from 6- to 384-wells• Measure kinetic rates of reaction• run multiplex assays quickly and easily• Save space on the benchtop with its very small footprint• Avoid costly maintenance with the robust, thoughtfully engineered optics and hardware• Never purchase costly absorbance filters

MultiFlo FX Dispenser/Washer

• Up to four independent reagents dispensed in parallel with no potential for carryover• unique parallel Dispense technologies – choice of peristaltic or syringe pumps for unique assay requirements• Large, high resolution color touch screen display• onboard computer with two uSB flash drive ports for file transfer, storage and operation• optional plate washing module increases functionality• Modular and upgradable• eLISAs, cell-based assays (including automated staining and fixing for imaging) and bead-based assays such as Luminex xMAp®

Promo code: NL-4/15Note! The promo code is required to obtain the special prices!

The offers are valid until 31.12.2015 and cannot be combined with other offers or agreements. The prices are listed excl. VAT and shipping charges.

We reserve the right to typos

BioNordikaBergman AS • tel 23 03 58 00 • fax 23 03 58 01 • info@bioberg.no • www.bionordikabergman.no

bergman

Our SErVICE TEaM

As a supplier of equipment and instruments to laboratories throughout the country, we do know how important it is that everything is working optimal.

Our skilled service engineers has all a high degree of competence within their product range and their expertise is kept at the highest level with continues trainings and courses at the suppliers.

Maintenance of instruments is crucial to make sure that they will give accurate and reliable results. Our service team follow the suppliers’ guidelines during the maintenance assignment.

Many of our customer have signed a periodic maintenance agreement that ensures ourfollow up and assistance. These agreements can be custom tailored depending on the equipment/instruments in your lab.

After a maintenance assignment, we set up a detailed report with information about the condition of the instrument, replaced spare parts and things that might need to be improved.

In addition to periodic maintenance, we also perform installations, repairs, calibrations and other technical assistance, either in our service facility in Gladengveien 3B or at your lab.

When did you last service your

spectrophotometer?

Should your plate reader have been

calibrated?

Maybe we can assist you with this or something else?

Should the filters in your water

purification system have been changed?