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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Bee Venom & Apitoxin
Version:
Th. Cherbuliez, MD & A. Kochan, MD
CMACC 2012 – Portland, OR
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
Bee venom and Apitoxin Comparisons : list of topics - page 1
Ease of keeping &
transporting
Dilutions possible
Acceptability to patient
Can be injected in hard tissue
With procaine, is not allergenic
Apitoxin Bee venom
Specialized enterprise
Variable dosages possible
Acceptability to patient requires
working with
Can be injected in hard tissue to a
degree
Trusting relation may decrease risks.
Injectable at any depth Depth limited to stinger’s
size
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
Bee venom and Apitoxin Comparisons : list of topics – page 2
Can be spread over large area
Specific molecule, identified action
Has small variability in potency
Can be used in research
Pain control, mixed with anesthetic
Can be spread over large area
Synergy of products and effects
Can be used in research
Pain control with different stings
Has always variability in composition
Apitoxin Bee venom
Loss of the “Energetics” of the bee “Energetics” conserved
Research re relative afficacy of Apit. No research relative efficacy of BV.
4/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
From Bee venom to Apitoxin a chromatographic study
Done in 2001 at Chemistry Department of the University of Louvain
in Brussels, Belgium
Conducted by Prof. R. Domerego of the Apitherapy Commission of
Apimondia
Comparison of fresh bee venom with Apitoxin
Description of the differences and inferences about their clinical
meaning.
5/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
From Bee venom to Apitoxin subtitle
Apitoxin from Michael Simics
Collection of fresh venom
Macerate of stingers in water 6 hours, with the presence of esterase
Without esterase the transformation takes 6 to 9 months
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Chromatography Collection Apparatus 2001
porous cylinder, with
Bees stinging tight junction
to Chomatograph
vacuum
warm water 80°
dry air 30° C
support
valve
Flow meter
tubing
Cool to - 90° C
T
°
© Apimondia Commission of Apitherapy Version:
7/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Chromatography-1 fresh Venom 140 bees
Compare 5,7,8,9,17,18,& 20 with apitoxin
34 spikes recorded in venom
4 & 6 represents 60% by mass
in fresh venom
© AAS & Apimondia Commission of Apitherapy Version:
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Chromatography-2 Apitoxin (M. Simics): 100 bees
very decreased ~ Disapeared New: R1,R2,R3,R4,R5 & R6
5 & 8
17,18 & 20
4
6
© AAS & Apimondia Commission of Apitherapy Version:
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Chromatography-3 macerate of 50 stingers for 6 hours
very decreased corresponding Generally decreased
17,18 & 20
5 & 8
© AAS & Apimondia Commission of Apitherapy Version:
10/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Chromatography Comparison Bee Venom / Apitoxine
© AAS & ApimondiaCommission ofApitherapy
34 spikes recorded in fresh bee venom
Spikes #4 & #6 most important in venom: 60% by weight
Spike #6 is at 6% in graphs 1 & 2 and 2% in graph 3
Spike #4 disappears in macerated venom sacs: graph 3
Spikes #5,7,8,9,17,and 20 disappear in graphs 3 & 4
Five new spikes appear in graphs 3 & 4
R1≈ D4, R2 ≈ D5, R3 ≈ D6, R4 ≈ D7 and R6 ≈ D9
11/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
From Bee Venom to Apitoxin
Version:
Major chemical transformation of the volatile components are
observed from venom to apitoxin and macerate.
The esters present in the venom are absent in the apitoxin and in
the macerate. Therapeutically, their action is antispasmodic,
calming, tonic, anti-arrhythmic.
Apitoxin and sting macerate are quite similar.
New acids appear in the apitoxin and in the macerate.
Instability of the apitoxin and the macerate.
12/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
Bee Venom Consultation One time Consultation; part 1
In was treated with 30 units (3000µg) of Apitoxin up to this consult
Man age ≈40; diagnosis MS; the “virulent” kind that kills in six months
I gave him 24 bees all on his back from coccyx to C7 (3600µg)
weight equivalent
He came on a stretcher in a light coma; he grunted when I talked to
him to his ear and in a loud voice
20 minutes later, he was eating an apple and talking with his family.
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
Bee Venom Consultation One time Consultation; part 2
...with the consent of the family I gave him the bee stings
What I did:
What I caused factually to happen:
I related to him to the extend he was available: minimal, then...
He received a cocktail of substances that his system had never
experienced and that was new by the richness of essential oils
contained in the venom
14/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
Bee Venom Consultation Reflection; part 3
The referring MD told me, had never seen such results
Look at the changes in his capacity to relate:
He related to his human environment:
He related to his physical environment:
He related to his body:
The change did not last. My treatment had no follow-up
Patient died two months later.
mother and young son
he was hungry
he ate an apple
15/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Apitoxin for Scarring
History
11/06/08 lumpectomy for CA of breast
12/12/08 mastectomy and clean-up of scar
01/08/09 Ist visit a lot of scarring and pain and
abduction limited to 90 degrees
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
01/08/09 Ist visit a lot of scarring and pain and abduction limited to 90 degrees
17/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
01/08/09 Ist visit a lot of scarring and pain and abduction limited to 90 degrees
18/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Treatment Course
01/08/09 0.5cc 25% Apitoxin 9 sites
01/09/09 0.5cc 25% Apitoxin 14 sites
Abduction 110 deg.
01/22/09 0.6cc 15 sites
01/29/09 0.6cc 15 sites 02/05/09 0.8cc 16 sites
Abduction 175 deg.!
12 more visits using 1cc or more completed on 06/12/09.
19/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
06/12/09 Treatment completed
20/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
Bee Venom Collector from Bulgaria
21/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D
INJECTABLE BEE VENOM
22/xx
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© Th. Cherbuliez, M.D. & A. Kochan, M.D xxxx
END