Benefits• Up to 24-Plex

2 bead sizes (S4) and (S5)

12 levels of fluorescence intensity for each bead size

• Works on flow cytometers equipped with a single blue or dual (blue and red) lasers

Uses the PE and either PE-Cy5 or APC detectors

No need to purchase and maintain another piece of expensive equipment

• Classic “Sandwich” style immunoassay format using capture (cAb) and detection (dAb) antibodies:

• Multiplexing – Less sample needed – only 15 µL!– Less assay time and cost

• Consistent and superior assay performance

• Flexible assay panels – “Mix and Match” one or more

individual analyte from a given Group to create a custom panel for your research

– Some assays can multiplex across Groups – contact YSL Bio for details

• 387 assays now available• Premixed standards and beads are

“ready to use” – less pipetting steps• Available in 32-test and 96-test kits

Assay Protocol Overview

24-Plex Assay

Correlation with Luminex™ Assays

Cell culture supernatantsamples from human PBMCstreated with various reagentswere assayed with the AimPlex®Human Th1/Th2/Th17 7-Plex*assay and a commerciallyavailable Luminex multiplexassay kit.

• Sample types: cell culture supernatant, saliva,

plasma, cell/tissue lysates, serum, BALF,

pleural and peritoneal fluids, and others

• LOD1: Majority of the assays < 10 pg/mL

• LLOQ2: Majority of the assays < 20 pg/mL

• ULOQ3: Majority of the assays > 5,000 pg/mL

• Standard dose recovery: 70-130%

• Intra-assay CV: < 10%; Inter-assay CV: < 20%

• Cross-reactivity of Panel analytes: negligible

• Sample volume: 15 µL

Assay Specifications

*Assays analyzed on a BD FACSCalibur™, Sony EC800™ or Beckman Coulter CyAn ADP™

Note: Trademarks are the property of their respective owners

For research use only. Not for use in diagnostic procedures.

Bead-based Multiplex Immunoassays for Flow

Representative Data Correlation with CBA ™ Assays

Size 5 Beads

Size 4 Beads

Peak#: 1 2 3 4 5 6 7 8 9 10 11 12

Peak#: 1 2 3 4 5 6 7 8 9 10 11 12

1. LOD: Limit of Detection (Sensitivity)2. LLOQ: Lower Limit of Quantification (Precision and Accuracy)3. ULOQ: Upper Limit of Quantification (Precision and Accuracy)

Human normal and abnormal serum samples were measured with both AimPlex® and CBA assays and analyzed on a BD FACSCanto II.

Your Resource for Customized Panels

Human Mouse

Available Assays*

Activin AANGPT-1/Angiopoietin-1BDNFbeta-NGF/NGFBBLyS/BAFF/sCD257/TNFSF13BBTC/BetacellulinCCL2/SYCA2/MCP-1CCL3/SYCA3/MIP-1 CCL4/SCYA4/MIP-1 βCCL5/SCYA5/RANTESCCL7/SYCA7/MCP-3/MARCCCL8/MCP-2CCL9/CCL10/MRP2/MIP-1CCL11/EotaxinCCL12/MCP-5CCL17/SYCA17/TARCCCL19/SCYA19/MIP-3 βCCL20/SCYA20/MIP-3 CCL21/Exodus-3/6CkineCCL22/MDC/ABCD-1CCL24/Eotaxin-2/MPIF-2CCL27/SCYA27/CTAKCCL28/MEC/CCK-1 CD14/sCD14CRP/C-Reactive ProteinCXCL1/GRO /KCCXCL2/GRO β/MIP2CXCL5/SCYB5/ENA78CXCL9/MIGCXCL10/IP-10CXCL13/BCA-1/BLCCXCL14/BRAK/MIP-2γCXCL16/SCYA16/HCC-4Endoglin/sCD105E-selectin/sCD62EFGF-1/FGF-acidic/HBGF1FGF-2/FGF-basic/HBGH2FGF-9/HBGF-9/GAFG-CSF/CSF-3GM-CSF/CSF-2HGF/HPTA/SFICAM-1/CD54IFN-IFN- R1/IFNGR1/sCD119IL-1 alpha/IL-1F1 IL-1 beta/IL-1F2 IL-1RAIL-1R1/sCD121aIL-2

β-NGF/NGFBCCL2/SYCA2/MCP-1CCL3/SYCA3/MIP-1 CCL5/SCYA5/RANTESCCL7/SCYA7/MCP-3 CCL11/EotaxinCXCL1/Gro /KCCXCL2/Gro β/MIP2CXCL6/SCYB6/GCP2CXCL10/IP-10CXCL12/SDF-1G-CSF/CSF-3GM-CSF/CSF-2ICAM-1/sCD54IFNIL-1 /IL-1F1IL-1 β/IL-1F2

IL-2IL-4IL-5IL-6IL-10/CSIF IL-12p40IL-12p70 IL-13IL-15IL-17A/CTLA-8 LeptinPDGF-ABTIMP-1TNF-alpha/TNF-TNFSF11/sCD254/RANKLVCAM-1/sCD106

Rat NHP Canineβ-NGF/NGFBCCL2/SYCA2/MCP-1CCL5/RANTESCCL7/MCP-3/MARCCCL11/EotaxinCXCL9/MIGCXCL10/IP-10CXCL12/SDF-1FGF basic/FGF-2IFNIL-1βIL-1RAIL-2IL-4IL-5IL-6IL-8/CXCL8

IL-10/CSIFIL-12p70 IL-17A/CTLA-8TGF-/TGF-type ITNF-

IFNγIL-2IL-6IL-8/CXCL8IL-10/CSIFIL-12p40IL-17AMCP-1/JE/CCL2SCF/MGF/KITLGTNFαVEGF

Sub-CategoriesHuman: Groups 1 – 10Mouse: Groups 1 – 5 Rat: Groups 1 and 2 Non-Human Primate: Group 1 Canine: Group 1

AdiponectinAFPALCAM/sCD166ANGPT-1/Angiopoietin-1Artemin/ARTN/NeublastinBDNFBetacellulin/BTCβ-NGF/NGFBBIGH3/TGFBI/βIG-H3 BLyS/BAFF/sCD257/TNFSF13BBMP-2

BMP-4

CA15-3/MUC1 CA19-9 CA125/MUC16 CA50 CCL1/SCYA1/I-309CCL2/SCYA2/MCP-1CCL3/SCYA3/MIP-1 CCL4/SCYA4/MIP-1 βCCL5/SCYA5/RANTESCCL7/SCYA7/MCP-3/MARCCCL8/SCYA8/MCP-2CCL11/SCYA12/Eotaxin-1CCL13/SCYA13/MCP-4CCL14/SCYA14/HCC-1CCL15/SCYA15/MIP-1 deltaCCL16/SCYA16/HCC-4CCL17/SYCA17/TARCCCL18/SCYA18/PARCCCL19/SCYA19/MIP-3 βCCL20/SCYA20/MIP-3 CCL21/SCYA21/6CkineCCL22/SCYA22/MDCCCL23/SCYA23/MPIF-1CCL24/SCYA24/Eotaxin-2CCL25/SCYA25/TECKCCL26/SCYA26/Eotaxin-3CCL27/SCYA27/CTAKCCL28/SCYA28/MECCD14/sCD14CD163/M130/sCD163 CEACAM-1/CD66aCEA/CEACAM-5/CD66eCNTFCRP/C-Reactive ProteinCX3CL1/FractalkineCXCL1/MGSA/GROαCXCL2/GROβ/MIP-2α/GRO2CXCL4/PF4CXCL5/SCYB5/ENA78CXCL6/GCP-3CXCL7/SCYB7/NAP-3CXCL9/MIGCXCL10/IP-10

CXCL11/I-TACCXCL12/SDF-1CXCL13/BCA-1CXCL14/BRAKCXCL16/SYCB16Cystatin C/CST3E-Cadherin/CDH1/sCD324EGFEGFR/ErbB1EG-VEGF/PROK1/PK1Endoglin/sCD105EndostatinEpCAM/MK-1/sCD326EPO-RE-selectin/ELAM-1/sCD62EFerritinFGF-1/FGF-acidic/HBGF-1FGF-2/FGF-basic/HBGH-2FGF-4FGF-7/KGFFGF-9/HBGF-9/GAFFGF-19Flt-3 Ligand/Flt-3L/Flk-2LFollistatin/FSTGalectin-1/GalaptinGASP-1/WFIKKNRPG-CSF/CSF-3GDF-15/MIC-1/PTGFBGDNF/ATF-1GM-CSF/CSF-2Gp130/sCD130/IL6STGranzyme A/GZMA /CTLA3Granzyme B/GZMB/CTL1HGF/Hepatopoietin AICAM-1/sCD54ICAM-3/sCD50IFN-2IFN-βIFN-IgAIgEIGF1IGF2IGFBP-1IGFBP-2IGFBP-3IGFBP-5IGFBP-6IGFBP-7IL-1 /IL1A/IL-1F1IL-1 β/IL1B/IL-1F2IL-1RAIL-1R1/sCD121aIL-1R2/sCD121bIL-2

IL-2R/sCD25IL-3IL-4IL-5IL-6IL-6R-/sCD127IL-7IL-8/CXCL8IL-9IL-10/CSIFIL-11IL-12 (p70)IL-12/IL-23 (p40)IL-13IL-15IL-16/LCFIL-17A/CTLA-8IL-17CIL-17FIL-19IL-20/IL-10DIL-21IL-22/IL-TIFIL-23/IL-23p19IL-24/MDA-7IL-25/IL-17EIL-27/IL-27AIL-28A/IFN-Lambda-2/IFN-λ2IL-29/IFN-Lambda-1/IFN-λ1IL-31IL-32IL-33/NFNEV/DVS27IL-34IL-35InsulinKeratin 19/KRT19/CK-19Klotho/KLA/KLLAP(TGF-β1)/Latent TGF-β1Leptin/LEPLIFLipocalin-2/LCN2/NGALLL37/CAMP/CAP18/FALL39L-selectin/LECAM-1/sCD62LLymphotactin/XCL1/SCYC1M-CSF/CSF1MIA/CD-RAPMidkine/MK/NEGF2MIFMMP-1/CLGN (Total)MMP-3/Stomelysin-1 (Total)MMP-7/PUMP-1 (Total)MMP-8/Collagenase 2 (Total)MMP-9/MANDP2 (Total)MMP-13/Collagenase 3 (Total)MPO

NCAM-1/sCD56NOV/CCN3/IGFBP-9NRG1-beta1/HRG1-beta1OPG/OsteoprotegerinOPN/Osteopontin/BNSP OSM/Oncostatin MPAPPAPD-1/sCD279PDGF-AAPDGF-ABPDGF-BBPDCD1LG1/PD-L1/sCD274PDCD1LG2/PD-L2/sCD273PECAM-1/sCD31PIGF/PIG-FP-selectin/LECAM-3/sCD62PRAGE/AGER/sRAGEResistin/ADSFSCF/MGF/KIT-LigandSLAMF4/2B4/NAIL/sCD244SLAMF8/BLAME/sCD353SLPIST2/IL-1R4/IL1RL1Survivin/BIRC5TGF-alpha/TGF-/TGFATGF-beta 1/TGF-β1/TGFBTHPO/Thrombopoietin/MGDFTIM-1/HAVCR-1/KIM-1TIMP-1/TIMP1TIMP-2/TIMP2TNF-alpha/TNF-TNF-beta/TNF-β/TNFSF1A/LTATNFRSF1A/sCD120a/sTNF R1TNFRSF1B/sCD120b/sTNF R2TNFRSF5/sCD40 TNFRSF6/sCD95/FasRTNFRSF9/sCD137/4-1BBTNFRSF10C/sCD264/TRAIL-R3TNFRSF13B/sCD267/TACITNFRSF14/sCD270/HVEMTNFRSF17/BCMA/sCD269TNFSF5/sCD154/sCD40LTNFSF6/sCD178/sCD95L/FasLTNFSF8/sCD153/sCD30LTNFSF10/sCD253/TRAILTNFSF11/sCD254/RANKLTNFSF12/TWEAK/Apo3 LigandTNFSF13/sCD256/APRILTNFSF14/sCD258/LIGHTTSLPVCAM-1/sCD106VEGF-AVEGF-CVEGF-D/FIGFVEGFR1/Flt-1VEGFR2/Flk-1/sCD309/KDR

IL-2Rα/sCD25IL-3IL-4IL-5IL-6IL-7IL-7R/IL-7Rα/sCD127IL-9IL-10/CSIFIL-11IL-12/IL-23 p40IL-12p70IL-13IL-15IL-17A/CTLA-8IL-17FIL-20/IL-10DIL-21IL-22IL-23p19IL-25/IL-17EIL-28A/IFN-Lambda-2/IFN-λ2IL-33/NFHEV/DVS27Leptin/LEPLIFL-selectin/sCD62LM-CSF/CSF-1 Neprilysin/sNEP/sCD10OPG/OsteoprotegerinOSM/Oncostatin MP-selectin/LECAM3/sCD62PRAGE/sRAGE/AGERTGF-beta 1/TGF-β1/TGFBTIMP-1/TIMP1TNF-alpha/TNF-TNFR1/sCD120TNFR2/sCD120bTNFRSF7/sCD27TNFRSF8/sCD30TNFRSF12A/sCD266/TWEAK-RTNFSF5/sCD155/sCD40LTNFSF8/sCD154/sCD30LTNFSF11/sCD254/RANKLTSLPVCAM-1/sCD106VEGFVEGFR1/Flt-1/sVEGFR1VEGFR2/Flk-1/sCD309/KDR