BC and Me – 1980 to 2003• 1980: First Meeting –NMR meeting• 1984: Joined Brit’s lab• 1984-87: Animal Models– Open Chest Animals: dog and cat

• Volume & pressure overload; ischemia; hypoxia– Closed Chest animals: dog

• Volume & pressure overload; cardiomyopathy; heart failure; ischemia

– Other intact organs: Skeletal Muscle; liver, brain• Increase work load; ischemia

• 1987-2003: Isolated organs, cells & mitochondria

Multiple Technologies

• NMR: 31P, 23Na; 1H; 13C; 19F• NADH Fluorescence• NIR Spectroscopy• Other physiological measurements– Piezoelectric crystals: Mechanical function– Laser Doppler: flow in blood vessels; tissue perfusion– Tissue electrodes: Cae2+, Ke+, Nae+– ECG, EEG– BP, HR, LVP

World Wide Colleagues• British Connection: John Haselgrove• Chinese Connection: Daning Zhang; DJ Wang• French Connection: Bertrand Autlier; Suzanne Wehrli• German Connection: Manfred Prammer• Hungarian Connection: Laci Ligeti; Tomas Ivanics; Aristied Kovach: Kati Megyeri; Laci Deszi• Indian Connection: Hari Subramanian; Ramin Mitra• Israeli Connection: Avraham Mayevsky; Myer Shinnar• Japanese Connection: Shoko Nioka; K Niibori• Netherland Connection: Hans van Beek• Polish Connection: Maria Erecinska; Krzysztof Wroblewski • Russian Connection: Andre Savchenko• Ukrainian Connection: Nicola Doliba; Natalya Doliba; Andriy Babsky• Uruguayan Connection: Juan Crestanello• US Connection: too numerous to list• The World: too numerous to list

NMR Spectroscopy – Multiple collaborations

• Intact in vivo brain• Closed Chest Animals: Dog; Cat• Open Chest animals: Dog; Cat• Perfused Heart• Superfused Cardiomyocytes; hepatocytes• Superfused Mitochondria – heart, liver

Closed Skull Cat Brain

Closed Skull Cat Brain – 31P

Open Chest Animal: Dog or Cat

Open Chest Dog – 31P

Closed Chest Dog – 31P

Langendorff Perfused In NMR

Isolated Perfused heart – 31P

Superfusion SystemIsolated Cells & Mitochondria

Isolated Superfused Cardiomyocytes -31P

Isolated Superfused Mitochondria- 31P

Optical Spectroscopy & Electrode System

• NADH Fluorescence– Heart – Brain

• NIR– Oxygenation

• Multiple electrode system– Ca2+– K+– Na+

NADH Fluorescence & NMR

NADH FluorescenceHypoxia & NE Administration

Multiparameter Analysis: Flow probe; NADH light guide; Balloon occluder

Multiparameter Analysis: Multi-channel Light Guide; Electrodes (Cae+, Ke+, Nae+; Laser Doppler

Tissue Flow; CA flow Probe

Multiparameter Analysis:NADH, Laser Doppler Flow; Ca2e+, K2+

Not the End – Just the Beginning

• The story continues through BC’s • Many students • Former colleagues • Friends