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Guy Hélin Co-founder, CEO [email protected]
Bacteriocins applications for the development of new genetic control of
microbial strains in industrial fermentation
Successful R&I in Europe 2019 - 10th European Networking Event Düsseldorf, Germany February 15, 2019
Our ambition: to be a leader in synthetic biology to improve yield and product quality by microbial control
Biocatalysis Recombinant products New selection technology developed by Syngulon (US patent 9,333,227 + 10,188,114) to control industrial microbes providing:
1. Gene containment
2. Yield increase by industrial Genetic Bio-control
3. Contamination prevention against microbial invaders
4. Genetic drift
Open / semi-open continuous production – could be multicellular Inside
Outside
Fighting contaminants with bacteriocins (can be produced in vivo or by chemical synthesis)
Immunity
Target
Gene of interest
Bacteriocin
Selection technology developed by Syngulon (US patent 9,333,227 + 10,188,114) to control microbes providing:
1. Bacteriocin selection of expressing clones
2. Yield increase
3. Control of genetic drift
4. 100% plasmid-based (available in most E coli strains)
Plasmid
Bacteriocin gene
Immunity gene
Closed and controlled batch fermentor
Immunity
Target
Gene of interest
Bacteriocin gene
Immunity gene
Bacteriocin
• 3 year Syngulon R&D project with support of Walloon region of Belgium • Purpose: development of a genetic firewall against contaminants • Research based on metagenomic analysis of industrial samples on a worldwide basis • For ethanol production based on sugar beet (EU), sugar cane (Brazil) and starch (EU), Syngulon
is working with Tereos, 2nd largest sugar producer WW
• (1) Tereos provides samples from 7 different industrial sites over a one year period • (2) Syngulon performs metagenomics analysis • (3) Syngulon develops bacteriocin cocktails to fight contaminants • (4) Tereos/Syngulon: test of solutions in R&D lab • (5) Tereos: test of solutions approved by R&D and regulatory in industrial sites
The PARAGEN research project
Metagenomic Data
• Type and role of partner(s) sought We would like to offer our technologies to any industrial partner using microbial fermentation that is looking at yield increase and prevention of microbial contamination.
• Previous experience in EU R&D&I projects Syngulon was member of the Proposal 745739 MasterMicro project (H2020-BBI-JTI-2016) that was favourably evaluated but not financed ( Excellence criteria 4.50, Impact 4.00, Quality and efficiency of the implementation 4.50).
Syngulon is looking for partners • For planned Horizon 2020 project • For ongoing Horizon 2020 project • For commercialization of EU-funded research results
R&D Partners
syngulon.com
SYNGULON Team Guy Hélin, Co-founder, CEO Dr. Philippe Gabant, Co-founder, CSO Dr. Mohamed El Bakkoury, CTO Yeast Bertrand Delahaye, R&D Scientist Thomas Gosset, Ir, R&D Engineer Dr. Johann Mignolet, R&D Project Manager Dr. Jason Bland, R&D Project Manager Dr. Juan Borrero, R&D Project Manager
Team / SAB / R&D Partners
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Scientific Advisory Board Pr Joseph Martial (Chairman), ULg, Liège (BE) Pr Bruno André, ULB, Brussels (BE) Adj-Pr Mike Chandler, University of Georgetown (USA) Pr Pascal Hols, UCL, Louvain-la-Neuve (BE) Pr Didier Mazel, Institut Pasteur, Paris (FR) Pr Laurence Van Melderen , ULB, Charleroi (BE) Pr Ruddy Wattiez, UMons, Mons (BE) IN MEMORIAM Dr Régis Sodoyer, ex-Sanofi Pasteur, Lyon (FR)
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