Bacterial Transformation With Plasmid DNAAdd plasmid* to 50 µl or 100 µl of competent cells in each of 2 tubes.

* Amount of plasmid and competent cells to be determined

Do not add plasmid to 50 µl or 100 µl of competent cells in each of 2 additional tubes.

Perform the following for each of the tubes of competent cells.

Incubate on ice for 15 minutes.

Heat shock at 42oC for 1.5 minutes.

Incubate on ice for 2 minutes.

Plate cells onto LB and LB/AMP plates.

Incubate at 37oC overnight.