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BACTERIA
• UBIQUITOUS IN NATURE• UNICELLULAR• LIGHT MICROSCOPIC - 0.2 - 2
MICROMETERS IN DIAMETER; 2 - 8 MICROMETERS IN LENGTH
• PROCARYOTIC IN CELL STRUCTURE• CELL SHAPE AND ARRANGEMENT
ANATOMY OF A TYPICAL BACTERIUM
• THE GLYCOCALYX – A SUGAR COAT
• CAPSULE, SLIME LAYER, BIOFILM
– PRODUCED WITHIN THE CELL AND SECRETED EXTRACELLULARLY
• CAPSULE
• CARBOHYDRATE - POLYSACCHARIDE
• WELL ORGANIZED
• LAYED DOWN EVENLY AROUND THE CELL WALL
• FIRMLY ATTACHED
• NOT EASILY PENETRATED
ADVANTAGES OF HAVING A CAPSULE
• ADHERENCE
• NUTRITION SOURCE• PROTECTION
– ANTIBIOTICS– IMMUNE SYSTEM– NUTRIENT AND WATER LOSS
SLIME LAYER
• NOT WELL ORGANIZED• EASILY PENETRATED• ADVANTAGES OF A SLIME LAYER
BIOLFILM
• BACTERIA LIVE IN COMMUNITIES CALLED BIOFILMS
• USUALLY ATTACHED TO SOMETHING – CATHETER, HEART VALVE, TOOTH, MUCOUS MEMBRANE
• ADVANTAGES OF A BIOFILM:
• PROTECTION FROM: DESSICATION, ANTIBIOTICS, BODY’S IMMUNE SYSTEM
• 70% OF HUMAN BACTERIAL INFECTIONS INVOLVE BIOLFILMS
THE BACTERIAL CELL WALL
• MAJOR COMPONENT OF MOST BACTERIAL CELLS
• MAINTAINS THE SHAPE AND INTEGRITY OF THE CELL
• PRESENT IN ALL PROCARYOTIC CELLS EXCEPT THE MYCOPLASMAS
• MAJOR DIFFERENCES IN CELL WALLS DEMONSTRATED BY THE GRAM STAIN
THE GRAM POSITIVE CELL WALL
• SINGLE LAYER• UP TO 90% PEPTIDOGLYCAN• 15-20 nm THICK• CONTAIN TEICHOLIC ACIDS WHICH ARE
ACIDIC POLYSACCHARIDES• CONTROL AUTOLYSINS IN THE CELL
THE GRAM NEGATIVE CELL WALL
• SEVERAL LAYERS SEEN• 10-15 nm THICK
• INTERESTING CHARACTERISTICS OF THE AMINO ACIDS IN PEPTIDOGLYCAN
• AMINO ACIDS CAN TAKE ON TWO STRUCTURAL CONFIRMATIONS IN SPACE
• D-AMINO ACIDS VS L-AMINO ACIDS
• DIAMINOPIMELIC ACID – AA FOUND ONLY IN PEPTIDOGLYCAN
• INTERESTING CHEMICALS THAT EFFECT THE CELL WALL OF BACTERIA.
• PENICILLIN• LYSOZYME
• DIFFERENCES BETWEEN THE CELL WALLS
OF THE ARCHAEABACTERIA AND THE EUBACTERIA.
• ARCHAEABACTERIA HAVE NO
PEPTIDOGLYCAN IN THEIR CELL WALLS• THERE ARE NO D AMINO ACID ISOMERS IN
THE CELL WALL OF THE ARCHAEABACTERIA. ONLY L AMINO ACID ISOMERS
• THE ARCHAEABACTERIA ARE GRAM VARIABLE
THE CELL MEMBRANE
• SIMILAR TO THAT OF EUCARYOTES• 50% PROTEIN AND 50% LIPID IN CHEMICAL
COMPOSITION• ACTIVE AND PASSIVE TRANSPORT
OBSERVED
• DIFFERENCES BETWEEN THE CELL MEMBRANES OF THE EUCBATERIA AND THE ARCHEOBACTERIA– OVERLAPPING LIPID LAYERS– DIFFERENT BONDING IN PHOSPHOLIPID
MOLECULES
CYTOPLASMIC CONTENTS
CYTOPLASMIC CONTENTS
• RIBOSOMES– MAKE UP MAJOR PART OF CYTOPLASM– 15,000+ PER CELL - POLYRIBOSOMES– 60% PROTEIN AND 40% RNA
• STORAGE GRANULES– CARBON RESERVE
• POLY-BETA-HYDROXYBUTYRATE• GLYCOGEN
– VOLUTIN– SULFUR
• ENDOSPORES
THE NUCLEAR APARATUS
• DNA• USUALLY A SINGLE CIRCULAR MOLECULE
OF DOUBLE STRANDED DNA• CAN BE LINEAR AS IN BORELLIA
BURGDORFERI
• EXTRACHROMOSOMAL DNA – PLASMIDS• DEFINITION OF A PLASMID• REPLICATES AUTONOMOUSLY• EASILY PASSED FROM BACTERIUM TO
BACTERIUM• PLASMID GENES ARE NOT NECESSARY
FOR THE SURVIVAL OF THE BACTERIUM• PLASMIDS USED IN GENETIC
ENGINEERING
EXTERNAL STRUCTURES OF THE BACTERIAL CELL
• FLAGELLA– CHEMICAL COMPOSITION - PROTEIN– ORGANELLE OF MOTILITY– DIAMETER IS BELOW THE RESOLVING
POWER OF THE LIGHT MICROSCOPE– ARRANGEMENT
• POLAR
• PERITRICHOUS
• FIMBRIAE - ADHESION
• PILUS - CONJUGATION
CLINICALLY SIGNIFICANT BACTERIA
A VARIETY OF CLINICALLY SIGNIFICANT BACTERIA AND THE DISEASES THEY CAUSE
• RODS - ARRANGEMENT– ESCHERICHIA COLI – STRAIN 0157:H7
• HUS-hemolytic – uremic - syndrome
– CLOSTRIDIUM– CORYNEBACTERIUM– MYCOBACTERIUM– LACTOBACILLUS– BACILLUS
• COCCI – ARRANGEMENT
• DIPLOCOCCI• NEISSERIA GONORRHOEAE – pp 790-792• NEISSERIA MENINGITIDIS – pp 645-646
• STREPTOCOCCI – pp 620-622;675-676; 714-715• STREPTOCOCCUS PYOGENES
– SCARLET FEVER– RHEUMATIC FEVER
• STREPTOCOCCUS PNEUMONIAE – pp 456;716• ENTEROCOCCUS FAECIUM - VRE (VANCOMYCIN RESISTANT
ENTEROCOCCUS) – pp 593– NOSOCOMIAL INFECTIONS
• TETRAD/SARCINAE - MICROCOCCUS LUTEUS
– STAPHYLOCOCCI• STAPHYLOCOCCUS AUREUS – pp 436;593;615-
20;675;751-2; MRSA, VISA, VRSA– NOSOCOMIAL INFECTIONS
• STAPHYLOCOCCUS EPIDERMIDIS
• STAPHYLOCOCCUS SAPROPHYTICUS
• SPIRALS• VIBRIO – CURVED RODS – VIBRIO
CHOLORAE – pp 755-758• SPIROCHETES
• TREPONEMA PALLIDUM – SYPHYLIS – pp 794-799
• BORRELIA BURGDORFERI – LYME DISEASE• pp. 685-687
BACTERIAL DIVERSITY• A VARIETY OF CLINICALLY SIGNIFICANT ATYPICAL
BACTERIA• CHLAMYDIAE – PHYLUM- pp 336-7;636;727;729;792-4• OBLIGATE INTRACELLULAR PARASITES• NO PEPTIDOGLYCAN IN THEIR CELL WALL• VERY SMALL RODS - 1.5 X 0.2 MICROMETERS• ENERGY PARASITES • TWO GENERA: CHLAMYDIA AND CHLAMYDOPHILA
• CHLAMYDIA TRACHOMATIS (STD)• EYE DISEASE SEEN OFTEN IN THIRD WORLD
COUNTRIES• CHLAMYDOPHILA PSITTACI – PSITTACOSIS• CHLAMYDOPHILA PNEUMONIAE
• RICKETTSIAE – PHYLUM -pp 316;687-688• OBLIGATE INTRACELLULAR PARASITES• VERY SMALL PLEOMORPHIC RODS – 0.8 X
2.0 MICROMETERS• TRANSMITTED FROM HOST TO HOST BY A
VECTOR – INSECTS AND TICKS• MOST PATIENTS PRESENT WITH A RASH• ROCKYMOUNTAIN SPOTTED FEVER
• MYCOPLASMAS – IN THE FAMILY MYCOPLASMATACEAE – pp 333-4; 726,794
• NO CELL WALL• FREE LIVING• VERY SMALL – SMALLEST FREE LIVING
ORGANISMS KNOWN• 0.3 – 0.5 MICROMETERS IN DIAMETER• CAN SLIP THROUGH 0.2 MICROMETER
MEMBERANE FILTERS• HAVE STEROLS IN THEIR CELL
MEMBRANES
• MYCOPLASMA PNEUMONIAE – WALKING OR ATYPICAL PNEUMONIA
• MYCOPLASMA HOMINIS - STD