1.90”

.43”

BACK PRESSURE REGULATORS (BPRS)

Proven Outgassing Protection

Flow-Independent Pump Preload for Greater Pump Efficiency

5 to 1,000 psi Cartridges and Assemblies Available

Upchurch Scientific® offers a full line of factory preset back pressure regulators, designed to enhance system performance through outgassing prevention and improved pump check valve efficiency.

Small gas bubbles often form as solvent moves from the high pressure of an HPLC column to the low pressure environment leading to the detector. This outgassing can cause erratic baseline readings and loss of sensitivity. Placing an Upchurch Scientific BPR after the detector provides an excellent, low-cost method for preventing this problem by maintaining enough back pressure on the mobile phase to keep gases dissolved in solution.

A back pressure regulator can also be used as a pump preload for low and fluctuating pressure applications. Many of today’s pumps require a steady back pressure to function properly. Install an Upchurch Scientific BPR (usually 500–1,000 psi) between the pump and the injector to enhance pump performance. Caution: Do not exceed the maximum operating pressure of your system — please refer to the operating manuals for your system components before choosing the appropriate BPR.

Our line of back pressure regulators includes:

5 and 20 psi Assemblies

40, 75, 100, 250, 500, 750 and 1,000 psi Cartridges and Assemblies

PEEK™ and Stainless Steel BPR Holders

High Pressure Adjustable BPR for pressures between 2,000 and 5,000 psi

Ultra Low Volume BPRs set to 100 and 500 psi (page 110)

Please Note: for flow control options try our Micro-Metering Valves found on page 105.

BPR ASSEMBLIESChoose from our line of PEEK and Stainless Steel BPR Assemblies, each complete with a replaceable, factory preset cartridge.

Upchurch Scientific BPR Assemblies create incremental back pressures ranging from 5 to 1,000 psi (0.3 to 69 bar). For low pressure applications, we recommend our 5 and 20 psi PEEK BPR Assemblies. These products are ideal for eliminating back pressure fluctuation in systems that have delicate components requiring a low pressure threshold. Since these two preset assemblies are extremely sensitive, they are sold as assemblies only – replacement cartridges are not sold separately.

PEEK Back Pressure Regulator Assembly, shown with available Cartridges

1.04”

1.92”

.42”

Back Pressure Regulators

U-609500 psi BPRshown with P-765 Cartridge

P-7905 psi BPR(sold as assembly only)

Back Pressure Re ulator Assembly:

Flangeless Ferrule

Cartridge Holder

Cartridge

Color-Coded End Cap

Flangeless Nut

Each BPR Assembly includes a preset BPR Cartridge and Upchurch Scientific fittings for 1/16” OD tubing.

BPR HOLDERSUpchurch Scientific P-465 PEEK and U-469 Stainless Steel BPR Holders work with any of our replacement BPR Cartridges. Each holder comes with fittings for 1/16” OD tubing (see below). The U-469 Holder is surface-treated to prevent galling, a potential problem with large, threaded metal parts.

Please Note: these PEEK Back Pressure Regulator Holders are designed to allow each cartridge to operate at its stated pressure setting when tightened to 20 in. lbs. of torque. To approximate this level of torque, first finger tighten the Holder, then tighten an additional 1/8–1/4 turn with the supplied wrenches.

P-76140 psi BPR Cartridge

P-7961,000 psi BPR Cartridge

REPLACEMENT BPR CARTRIDGESUpchurch Scientific® BPR Cartridges have a maximum internal volume of 146μL. The cartridge bodies are manufactured from inert PEEK™ and Tefzel® (ETFE) polymers. Internal components are manufactured from PEEK, highly fluorinated elastomer and gold-plated stainless steel. The replacement cartridges will operate in any of the holders listed on pages 108 and 109. These cartridges create back pressures from 40 to 1,000 psi (2.8 to 69 bar) — all independent of flow except as noted below.

Please Note: the recommended operating flow rate range for our BPR Cartridges is 0.1mL - 10mL/min. Within this range, the amount of back pressure created by the BPR Cartridges and Assemblies will not vary more than ±10%. Lower or higher flow may result in larger pressure fluctuations.

HIGH PRESSURE ADJUSTABLE BPRThe biocompatible P-880 High Pressure Adjustable BPR offers the flexibility to adjust your system back pressure between 2,000 and 5,000 psi (138 and 345 bar), independent of the flow‡. To achieve the desired back pressure setting, simply turn the thumbscrew while monitoring your system pressure. Because this product creates such high back pressure, please check system component specifications prior to using to avoid damaging any sensitive components.

‡ Only 10% fluctuation in pressure generally occurs with flow rates of 0.1 - 10mL/min. Lower or higher flow rates will lead to greater fluctuations in pressure.

Back Pressure Regulators

BPR ASSEMBLIES WITH PEEK HOLDER

Swept VolumeP-790 5 psi (0.3 bar) BPR cartridge with PEEK holder1 134μL $ 79.10P-791 20 psi (1.4 bar) BPR cartridge with PEEK holder1 134μL 79.10P-785 40 psi (2.8 bar) BPR cartridge (P-761) with PEEK holder2 131μL 79.10P-786 75 psi (5.2 bar) BPR cartridge (P-762) with PEEK holder2 131μL 79.10P-787 100 psi (7 bar) BPR cartridge (P-763) with PEEK holder2 131μL 79.10P-788 250 psi (17 bar) BPR cartridge (P-764) with PEEK holder3 102μL 79.10P-789 500 psi (34 bar) BPR cartridge (P-765) with PEEK holder4 96μL 79.10P-455 1,000 psi (69 bar) BPR cartridge (P-796) with PEEK holder4 89μL 79.10

BPR ASSEMBLIES WITH STAINLESS STEEL (SST) HOLDER

Swept VolumeU-605 40 psi (2.8 bar) BPR cartridge (P-761) with SST holder5 129μL $ 104.20U-606 75 psi (5.2 bar) BPR cartridge (P-762) with SST holder5 129μL 104.20U-607 100 psi (7 bar) BPR cartridge (P-763) with SST holder5 129μL 104.20U-608 250 psi (17 bar) BPR cartridge (P-764) with SST holder5 99μL 104.20U-609 500 psi (34 bar) BPR cartridge (P-765) with SST holder5 93μL 104.20U-610 750 psi (52 bar) BPR cartridge (P-795) with SST holder4 91μL 104.20

REPLACEMENT CARTRIDGES

COLOR CODING Swept Body End-Cap VolumeP-761 40 psi (2.8 bar) BPR cartridge Tan Blue 125μL $ 54.45P-762 75 psi (5.2 bar) BPR cartridge Tan Yellow 125μL 54.45P-763 100 psi (7 bar) BPR cartridge Tan Red 125μL 54.45P-764 250 psi (17 bar) BPR cartridge Tan White 95μL 54.45P-765 500 psi (34 bar) BPR cartridge Tan Green 89μL 54.45P-795 750 psi (52 bar) BPR cartridge Black Blue 87μL 54.45P-796 1,000 psi (69 bar) BPR cartridge Black Green 83μL 54.45

BPR HOLDERS

P-465 Biocompatible PEEK BPR holder4 $ 53.35U-469 High Pressure Stainless Steel BPR holder6 75.60

HIGH PRESSURE ADJUSTABLE BPR ASSEMBLY

Swept VolumeP-880 2,000 - 5,000 psi (138-345 bar) Adjustable BPR7 9μL $ 123.55

1 Comes with P-200 ETFE Flangeless Ferrules and P-215 ETFE Flangeless Nuts. 2 Comes with P-200 ETFE Flangeless Ferrules, P-215 ETFE Flangeless Nuts and wrenches. 3 Comes with P-200 ETFE Flangeless Ferrules, P-235 PEEK Flangeless Nuts and wrenches. 4 Comes with LT-115 Super Flangeless PEEK Nuts, P-250 Super Flangeless Ferrules and wrenches. 5 Comes with P-200 ETFE Flangeless Ferrules and P-201 Delrin® Flangeless Nuts. 6 Comes with F-300 PEEK Two-Piece Fingertight Fittings. 7 Comes with F-120BLK One-Piece Fingertight Fittings.

.73”

1.08”

P-880High Pressure Adjustable BPRIncludes One-Piece Fingertight Fittings for 1/16” OD tubing

Out

In

BPR Holders:

U-469Stainless Steel BPR Holder(cartridge sold separately)

F-300Two-Piece Fingertight Fitting(page 14)

* Using PEEK tubing and supplied fittings. ** Using stainless steel tubing and supplied fittings.

Pressure Rating 3,000 psi (207 bar)* Pressure Rating 4,000 psi (276 bar)**

P-465PEEK BPR Holder(cartridge sold separately)

LT-115LiteTouch® Nut(page 19)

P-250Super Flangeless™ Ferrule(page 19)

ULTRA-LOW VOLUME BPRs

M-410 100 psi (7 bar) Low Flow, with PEEK Tubing (.010” ID x 1/16” OD) $ 108.50M-412 500 psi (34 bar) Low Flow, with PEEK Tubing (.010” ID x 1/16” OD) 108.50M-420 100 psi (7 bar) High Flow, with PEEK Tubing (.020” ID x 1/16” OD) 108.50

PRESSURE RELIEF VALVES

Swept VolumeU-455 5 psi (0.3 bar) Pressure Relief Valve1 for 1/16” OD tubing 148μL $ 108.85U-456 100 psi (7 bar) Pressure Relief Valve2 for 1/16” OD tubing 139μL 108.85P-612 Pressure Relief Valve Tee1 for 1/16” OD tubing 14μL 52.70P-612S Pressure Relief Valve Tee3 for 3/16” OD tubing 348μL 52.70

All Back Pressure Regulators listed above ship complete with P-230 Flangeless Nuts and P-200 Ferrules (page 56). 1 Comes with P-201/P-200 Flangeless Fittings. 2 Comes with P-201/P-200 Flangeless Fittings and wrenches. 3 Comes with P-133/P-132 Flangeless Fittings.

ULTRA-LOW VOLUME BPRS Our Ultra-Low Volume Back Pressure Regulators (BPRs) were developed to minimize swept volume, which is especially important for multidetector applications. With a maximum swept volume of only 6μL*, it is nearly impossible to detect these BPRs as part of your fluid pathway (please see “Difficult to Detect” below for more details). And because the flow path is completely polymeric, you are assured of biocompatibility.

Four Ultra-Low Volume BPRs are available with back pressure settings of 100 and 500 psi (see Specifications Table). These units can be operated above and below their flow-rate recommendations; however, the resulting back pressure will differ from that set at the factory.

A set screw allows adjustments to the back pressure rating. Take care to ensure the resulting back pressure does not exceed the recommended pressure range listed in the Specifications Table or that of your system components.

To minimize the swept volume added to your flow path, we recommend trimming the length of the attached tubing.

Please Note: Our Ultra-Low Volume Back Pressure Regulators cannot be used as check valves due to their unique internal design. Try our Micro-Volume Inline Check Valve on page 112.

* The maximum internal swept volume listed above is for the back pressure regulator only and does not include the volume of the attached tubing lines.

P-612Pressure Relief Valve TeeIncludes Flangeless Fittings for 1/16” OD tubing

U-455 Pressure Relief ValveIncludes Flangeless Fittings for 1/16” OD tubing

1”

2.50”

BPRs and Pressure Relief Valves

PRESSURE RELIEF VALVES Prevent System Over-Pressurization

Upchurch Scientific Pressure Relief Valves are ideal for preventing system over-pressurization. These products protect system components by diverting fluid flow out of the tee when pressure exceeds the set limit. Choose between our preset 100 psi (7 bar) and 5 psi (0.3 bar) assemblies, both shipped with our Flangeless Fittings. The 100 psi version is a good, general purpose valve, while the 5 psi version is perfect for protecting syringe and peristaltic pump systems (see pages 136 – 143). The void volume of both relief valves is low due to the small .020” (.50mm) thru-holes in the valve tee body.

If you wish to have the Pressure Relief Valve open at a different pressure than 5 or 100 psi, simply combine one of the other replacement Back Pressure Regulator Assemblies listed on page 109 with the P-612 Pressure Relief Valve Tee. Choose the P-612S for larger bore tubing and higher flow applications.

A sample of identical concentration was injected and data was collected from two detectors simultaneously. In the first example below, the two detectors were separated by 42cm of .010” ID tubing, and in the second example they were separated by the Upchurch Scientific® Ultra-Low Volume BPR with 60cm of .010” ID PEEK™ tubing attached. As the data indicates, the presence of the BPR is barely detectable!

Flow Rate = 300μL/min - Column = 2.0mm ID x 15cm I. Detectors with 42cm of .010” ID tubing between them: Retention Peak Peak Width Detector Peak Time Area Asymmetry at 1/2 Height#1 2 7.56 129305.8 1.33 0.30#2 2 7.71 132467.62 1.44 0.32

II. Detectors with M-410 Ultra-Low Volume BPR and 60cm of .010” ID tubing between them:

Retention Peak Peak Width Detector Peak Time Area Asymmetry at 1/2 Height#1 2 7.66 127022.48 1.34 0.30#2 2 7.85 130727.53 1.38 0.33

Data supplied by Gilson®, Inc.

Difficult to Detect:

Ultra–Low Volume BPR Specifications

1:

Back Recommended Pressure Flow Rate Pressure 1/16” OD Setting Recommendations Range TubingM-410 100 psi2 Optimal: 100μL–1mL/min 40-150psi PEEK, .010” ID (7 bar)2 Max.: 4mL/min (3-10 bar)M-412 500 psi2 Optimal: 100μL–1mL/min 250-525psi PEEK, .010” ID (34 bar)2 Max.: 4mL/min (17-36 bar)M-420 100 psi3 Optimal: 3–8mL/min 40-150psi PEEK, .020” ID (7 bar)3 Max.: 10mL/min (3-10 bar)

1 All data generated using water at room temperature. 2 Set at a flow rate of 0.5 mL/min. 3 Set at a flow rate of 5 mL/min.