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BIOMATERIALS LAB INTRODUCTION AND SAFETY
Wednesday, January 18th
BME 245/445 – Lab 0
PREFACE
The goal of this course is to provide students with hands on experience in…
Materials synthesis Materials characterization Materials modification Cell culture Histological Analysis Technical Writing & Data Analysis
01/18/2012
EXPECTATIONS
SAFETY! arrive prepared for lab work in TEAMS of 4-5 to complete lab write INDIVIDUAL lab report provide constructive feedback
01/18/2012
OVERVIEW
Safety Lab Outline Expectations Reports
01/24/2012
SAFETY
Environmental Health & Safety Training for Biological/Chemical Research Labs
labs are dangerous; so be safe if you have questions…ASK DON’T BE STUPID
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CHEMICAL HAZARDS
Flammable / Combustible / Toxic Respect all chemicals in lab
If you can smell it…it is bad for you! Use a hood if specified
LABEL EVERYTHING!
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BIOHAZARDS
Biosafety Level 1 – least hazardous Cell Culture Media Preparation
LABELS!
(krishgen.com)
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PROTECTIVE EQUIPMENT
Goggles – on at ALL times! Gloves – use them for everything Closed-toe shoes
Safety Quiz
(sentrysafetysupply.com)
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BIOMATERIALS LABS
1. Biomaterial Mechanics
2. Bulk vs. Surface Degradation
3. Cell-Material Interactions
4. Controlled Delivery and Release
5. Histological Analysis of Implanted Biomaterials
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Investigate how hydrogel precursors influence end-stage network mechanics
1. BIOMATERIAL MECHANICS
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Investigate different network chemistries and how these influence degradation
2. DEGRADATION MECHANISMS
(Wikipedia - Anhydride. 2011)
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3. CELL INTERACTIONS
Investigate how hydrogel incorporated “RGD” peptide influences cell adhesion
01/24/2011
(U. Michigan ME – Jianping Fu. 2011)
4. DELIVERY AND RELEASE Investigate how hydrogel properties can be
modified to control drug delivery
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Small Mesh Size Large Mesh Size
5. HISTOLOGICAL ANALYSIS OF
IMPLANTED BIOMATERIALS
Investigate how tissue responds to implanted biomaterials
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(Heungsoo Shin et al. Biomaterials. 24(19) 2011)
PRE-LAB
Literature reading relevant to lab Calculations Brief quiz at start of lab Introductory “lecture”
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POST-LAB REPORTS
Present findings in clear/concise fashion Make connections between lab experiments
and pre-lab literature reading List team members Error analysis REFERENCES! See Write-up Expectations on Blackboard
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For Lab #1
Register: Mon or Tues only Complete safety quiz; bring to lab (104)
Read paper on Blackboard Read lab handout Do pre-lab calculations