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8/16/2019 b-hcg.pptx

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Beta HCG d

Progesteron

disusun oleh

Linda Ayu(3

Widia Utam

Dian Lisnaw

Meisya TrinSalisa Husn
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HCG (Human Chorionic

Gonadotropin)

- Hormon glikoprotein yang biasanya dihasilkan oleh plasentkehamilan- HCG disekresi syncytiotrophoblast kedalam sirkulasi darah saat implantasi hari keenam sampai ketujuh setelah fertilisas- HCG mendukung korpus luteum menghasilkan hormon pro

mempertahankan kehamilan- HCG juga diproduksi oleh cancer pada GIT, UGT, bronchial

juga limfoma
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Sub unitHCG

!" HCg #$ tidak spesi%kkarena hampir identik dengan subunit

alpha hormon glikoprotein lainnya,seperti Thyroid Stimulating Hormone&TSH', !uteini(ing Hormone &!H', dan"ollicle Stimulating Hormone &"SH')

*eta HCg#$ Spesi%kSehingga beta-hCG

memungkinkan deteksi dinikehamilan
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+emeriksaan dilakukan untuk )

aluasi molahidatidosa

.ehamilan

Chorio-carsinoma

*ahan pemeriksaan) Serum/ 0angan gunakan hemolitik, ikterik atau spesimen liHarap dicatat) Sampel yang mengandung natrium a(ida karena tidak boleh digupengujian tersebut/ Nilai rujukan : < 5 mIU/ml
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1eningkat +ada ) 1enurun +

2/ .ehamilan3/ 1ola hidatidosa4/ Chorio-carcinoma5/ Teratoma oarium atau teratoma

testis

6/ .ehamilan ganda7/ Seminoma, kanker kolon, kanker

gaster,kanker paru, kankerpayudara

8/ .ehamilan topic9/ ritroblastis feotalis

2/ bsortus mengan3/ .ematian janin

4/ Gestosis5/ .ehamilan ektop6/ IU":
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Prinsip Pemeriksaan Beta

dengan Elisa metode Sand

ntibody Capture

;ells dilapisi denganantibodi anti-
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a/ +rosedur kerja

2/ :iberi penomeran pada mikro>ell

3/ :ipipet 36 ul standar, control, dan sampel denganmenggunakan tip disposible kedasar >ell/

4/ Tambahkan 2?? ul en(ym konjugat/

5/ Campurkan selama 2? detik/

6/ Inkubasi selama 7? menit disuhu ruang/

7/ .eluarkan kelebihan reagen dari mikro>ell, beeri 5??ul a@uadest kedalam >ell selama 6 menit/

8/ Tambah 2?? ul substrat /9/ Inkubasi 26 menit pada suhu ruang/

A/ *eri stopsolution 6? ul/

2?/ :ibaca B: pada panjang gelombang 56? 2? nm/
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C/ +erhitungan Hasil &kualitatif'Untuk analisis kualitatif tingkat arna spesimen dibandingkan

dengan >arna ? mIU D m! dan 6? standar mIU Dm!/- 0ika >arna biru kurang intens dibandingkan>arna 6? mIU D m! standar, sampel dianggapsebagai negatif/

- 0ika >arna biru lebih intens dari atau samadengan >arna 6? mIU D m! standar sampeldianggap sebagai positif/
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Progesteron

disekresi ole korpus luteum dan plasenta $

kegunaan : a$ #iosintesis steroid pada semua jaringan yang mengeluarkan ormo

#$ *rogesteron #ertanggung ja)a# untuk peru#aan progestasional d

dan peru#aan siklik dalam leer raim dan .agina $

E %ujuan pemeriksaan : %ingkat progesteron plasma #erguna dalam menyeliddan pemantauan o.ulasi $
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1eningkat +ada )1enurun +a

2/ .ehamilan3/ Bulasi4/ .ista oarium

5/ Tumor adrenal6/ Tumor oarium7/ 1ola hydatidosa8/ Congenital adrenal hiperplasia

2/ menorrhoe

3/ bsorbsi mengan4/ .ematian janin5/ klamsi
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Prinsip

pemeriprogest

kompet
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