Ayush in Cancer Care (Compilation Work)

7/25/2019 Ayush in Cancer Care (Compilation Work)

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AYUSH FOR CANCER CARE

Abstracts

Compendium of Select Research Papers

(Full text of Papers in CD)

CENTRAL COUNCIL FOR RESEARCH IN AYURVEDIC SCIENCES

Ministry of Ayurveda, Yoga & Naturopathy, Unani, Siddha,

Homoeopathy (AYUSH)

Govt. of India

New Delhi


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IndexSI.

NO.

Content Page

No.

I. CONCEPT PAPER

II. RESEARCH PUBLICATIONS

2.1 Clinical Research 5

2.1.1 Treatment of Relapsed Undifferentiated Acute Myeloid Leukemia (AML-MO) with Ayurvedic Therapy

Balendu Prakash,International Journal of Ayurveda Research Vol.2, Issue1,Jan-Mar 2011

6

2.1.2 Taming the Side Effects of Radiotherapy in Oral Carcinoma Patients withHelp of Ayurvedic Medicine (Clinical Study)

Dr.Sunil Gupta, Dr.H.K.Kushwaha,Dr. Amita Jhunjhunwala, Dr. D.P. Agarwal,Journal of

Ayurveda Vol.ii,No.4,Oct.-Dec.-2008

7

2.1.3 Effect of Intervention of Ayurveda and Practical Implementation ofShadchakras(Pranic Healing) an Indigenous Approach as Co-Therapy with

Chemotherapy & Radiotherapy for Physical & Mental well being of Cancer

Patients- a clinical study

Dr.Pooja Sabharwal,Dr.M.Dinkar Sarma,Dr.D.P. Agarwal Journal of Ayurveda Vol.ii,No.4,Oct.-Dec.-2008

8

2.1.4 An Effective Herbomineral Treatment as an Adjuvant Therapy for theImprovement of Quality of Life of Hepatocellular Carcinoma(HCC)

Jayawardhane N.D.N.,De Silva R.H.S.K.,Tripathi J.S., Narasimha Murthy k.h.h.v.s.s.,Tiwari s.k.Jayawardhane n.d.n et al.irjp 2012, 3:(7).

9

2.1.5 Protective Effect of Yashtimadhu(glycyrrhiza glabra) Against SideEffects of Radiation/Chemotherapy in Head and Neck Malignancies

Debabrata Das,S.k.Agarwal,H.M.Chandola, AYU/Vol.32,Issue 2,Apr-Jun 2011

10

2.1.6 Efficacy of Rasayana Avaleha as Adjuvant to Radiotherapy andChemotherapy in reducing Adverse Effects

Purvi vyas,A.B.Thakar, M.S.Baghel,Arvind Sisodia,Yogesh Deole,AYU/VOL.31,Issue4,Oct.-Dec.2010

11

2.1.7 Efficasy Of Varunadi Ghritha (Polyherbal Compound) In Treated HeadAnd Neck Cancer As A Biological Response Modifier

Divya Ravindran,Indhu Hariharan,Richard Muwonge, Rejnish R.Kumar, M. RadhakrishnaPillai,Kunnambath Armadas, Ayu/Vol.35,Issue2,Apr-Jun2014

12

2.1.8 Low Resource Screening method of Pre-Cancerous Lesions and itsreversal by Triphala in teen-age Indian Population

Anshula Deshpande, Shobha Tendon, Neeraj deshpande,Ayu/Vol.35,Issue2,Apr-Jun2014

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2.1.9 Swarna Bhasma in Cancer: A Prospective Clinical Study

Soumen das, Mangal c.das, Retina Paul,Ayu,Vol.33,Issue3,Jul.-Sep.2012

14

2.1.10 Evaluation Of Clinical Response Of Carcinolyt (Herbal Nutrients) ToControl Adverse Effects Of Radiotherapy In Cancer Patients

Dr. Amanpreet Broca, Dr.Umesh Chandra Sharma, Prof. Hemant Kumar Kushwaha,ProflS.S.Sharma, Dr. D.P. Agarwal,Dr. Arun Chauguley,JOURNAL Of AYURVEDA VOL.1, No.1,Jan-

Mar2007

15

2.1.11 Yogas Impact On Inflammation,Mood,And Fatigue In Breast CancerSurvivors: A Randomized Controlled Trial

Janice K.Kiecolt-Glaser,Jeanette M. Bennett, Rebecca Andridge, Juan Peng, Charles L. SHAPIRO.William B. Malarkey,Charles F.Emery,Rachel Layman, Ewa E.Mrozek,And Ronald Glaser,YogaFor Breast Cancer Survivors,Page1-12

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2.1.12 Effect of Yoga Interventions on Fatigue in Cancer Patients andSurvivors: a Systematic Review of Randomized Controlled Trials

Julie Sadja,Paul j.Mills,Sadja and Mills ,Page1-19

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2.1.13 Classical Homeopathy in the treatment of Cancer PatientsAProspective Observational Study of Two Independent Cohorts

Matthias rostock,johannes naumann,corina guethlin, lars guenther, hans h bartsch ,herald walach,Rostock et al. Bmc cancer 2011, 11-19.

18

2.2 Pharmacology, Experimental Studies and Standardization

2.2.1 Report on Screening of Single Herbal Drug Extract for Potential Anti-Cancer Activity

Published by CCRAS, Department of AYUSH, Govt. of India, New Delhi, in 2009

20

2.2.2 Ayurvedic Drugs in the management of Cancer.

Published by CCRAS, Department of AYUSH, Govt. of India, New Delhi, in 1999

22

2.2.3 Evaluation of some plant extracts for standardization and anti canceractivity.

S.N. Gaidhani, Arjun Singh, Suman Kumari, G.S.Lavekar, A.S. Juvekar, S. Sen & M.M.Padhi.,Ind.J. Traditional Know. Vol. 12 (4), Oct. 2013, PP. 682-687.

24

2.2.4 In-Vitro Anticancer Activity of Standard Extracts Used In Ayurveda

S.N. Gaidhani , G.S Lavekar, A.S. Juvekar, S. Sen, Arjun Singh, Suman Kumari., PHCOGMAG.,Vol 5, Issue 20 (Suppl.), Oct-Dec, 2009 Page 425-429.

25

2.2.5 Screening the Anti-Cancerous Efficacy of Achyranthes aspera Linn. usingAnimal ModelSwiss Albino Mice

Geetha. P, Narayanan. K.R and A.G. Murugesan, J Biomed Sci and Res., Vol 2 (4), 2010, 231-235.

26

2.2.6 Plants as a source of anti-cancer agents.

Gordon M. Cragg

, David J. Newman.Journal of Ethnopharmacology 100 (2005) 7279.

27

2.2.7 Preparation And Standardization Studies On Veera Mezhugu A Siddha 28


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Anticancer Formulation.

P Rajalakshmi, C Savariraj Sagayam , P Brindha, International Journal of Pharmacy andPharmaceutical Sciences, Vol 6, Suppl 1, 26-33.

2.2.8 Pharmacognostic, Preliminary Phytochemical Studies And AnticancerousPotential Of Trigonella Foenum-Graecum.

G. Chauhan, M. Sharma, H. Kharkwal and A. Varma, Pharma Science Monitor An International

Journal Of Pharmaceutical Sciences, Vol-2, Issue-2, 2011, 72-81.

29

2.2.9 Comparative Anticancer Potential of Clove (Syzygium aromaticum) - anIndian Spice - Against Cancer Cell Lines of Various Anatomical Origin.

Vinay Dwivedi, Richa Shrivastava, Showket Hussain, Chaiti Ganguly Mausumi Bharadwaj, AsianPacific Journal of Cancer Prevention, Vol 12, 2011, 1989-1993.

30

2.2.10 Cytotoxicity, Toxicity, and Anticancer Activity of Zingiber OfficinaleRoscoe Against Cholangiocarcinoma.

Tullayakorn Plengsuriyakarn, Vithoon Viyanant, Veerachai Eursitthichai, Smarn Tesana, WannaChaijaroenkul, Arunporn Itharat, Kesara NaBangchang, Asian Pacific Journal of Cancer

Prevention, Vol 13, 2012, 4597-4606.

31

2.2.11 Potential of traditional ayurvedic formulation, Triphala, as a novelanticancer drug.

T. Sandhyaa, K.M. Lathika a, B.N. Pandeyb, K.P. Mishra.Cancer Letters 231 (2006) 206214.

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2.2.12 Aloe-emodin novel anticancer Herbal Drug

Khemkaran Ahirwar, Sanmati K. Jain, International Journal of Phytomedicine 3, (2011) 27-31Phytomedicine 3 : 2 (2011).

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2.2.13 In Vitro Anticancer Activity of the Root, Stem and Leaves of Withania

Somnifera against Various Human Cancer Cell Lines.

B. Yadav, A. Bajaj, M. Saxena, and A. K. Saxena, Indian J Pharm Sci. 2010 Sep-Oct; 72(5): 659663

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2.2.14 In vitro evaluation of anticancer and antimicrobial activity fromAyurveda.

Rajesh N. Gacche *, Rafik U. Shaikh, Mahesh M. Pund, Asian Journal of Traditional Medicines,2011, 6 (3)

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2.2.15 Preliminary Anticancer Screening and Standardization of SomeIndigenous Medicinal Plants using Cell Biology and Molecular Biotechnology

based models.

S.kavita Bagya, P.V Rajashree and Kishore Gnana Sam, Research Journal of Medicinal Plant5(6):728-737,2011.

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2.2.16 Ursolic Acid and Oleanolic Acid Suppress Preneoplastic Lesions Inducedby 1,2-Dimethylhydrazine in Rat Colon.

Ricardo A. Furtado, rlon P. Rodrigues, Felipe R. R. Arajo, Wendel L. Oliveira, Michelle A.Furtado, Mrcio B. Castro, Wilson R. Cunha and Denise C. Tavares.Toxicologic Pathology, 36: 576-580, 2008.

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2.2.17 A Double-Blind Study on the Effects of Differing Purified Cellulose andPectin Fiber Diets on 1,2-Dimethylhydrazine-induced Rat Colonic Neoplasia.

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Hugh J. Freeman, Gene A. Spiller and Young S. Kim, [CANCER RESEARCH 40, 2661-2665, August1980]

2.2.18 Chemoprevention of Colon Carcinogenesis by Organosulfur Compounds.

Bandaru S. Reddy, Chinthalapally V. Rao, Abraham Rivenson, CANCER RESEARCH 53. 3493-3498. August I. 1993

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2.2.19 Chemopreventive potentialof ferulic acid in 7,12-

dimethylbenz[a]anthracene-induced mammary carcinogenesis in SpragueDawley rats.

Nagarethinam Baskaran, Shanmugam Manoharan, Subramanian Balakrishnan, PachaiappanPugalendhi, European Journal of Pharmacology 637 (2010) 2229.

39

2.2.20 Chemoprevention of tea on colorectal cancer induced bydimethylhydrazine in Wistar rats.

Xu Dong Jia and Chi Han, World Journal of Gastroenterology, 2000; 6(5):699-703.

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2.2.21 Chemoprevention of Colon Carcinogenesis by Dietary Curcumin, aNaturally Occurring Plant Phenolic Compound

Chinthalapally V. Rao, Abraham Rivenson, Barbara Simi, et al., CANCERRESEARCH55, 259-266,January 15, 1995

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2.2.22 Decrease of intestinal tumors induced by 1,2-dimethylhydrazine in ratsfed with cow milk and buffalo milk.

M. Snchez Negrette, M.A. Montenegro, M.S. Catuogno, W.J. Lrtora, And M.C. Guanziroli,BIOCELL 2007, 31(3): 391-396.

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2.2.23 Effect of ginger on lipid peroxidation and antioxidant status in

1,2dimethyl hydrazine induced experimental colon carcinogenesis.

V Manju, N Nalini, J. Biochem Tech (2010) 2(2):161-167.

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2.2.24 Effect of kaempferol on lipid peroxidation and antioxidant status in 1,2-dimethyl hydrazine induced colorectal carcinoma in rats.

Parthasarathy Nirmala, Manickam Ramanathan, European Journal of Pharmacology 654 (2011) 7579.

44

2.2.25 Inhibition by Ginseng of Colon Carcinogenesis in Rats.

Shoji Fukushima, Hideki Wanibuchi, Wei Li, J Korean Med Sci 2001; 16(Suppl): S75-80.

45

2.2.26 Inhibitory effect of whole oat on abbrant crypt foci information and colontumour growth in ICR and BALB/cmice.

Hsueh-Chun Wang a, Chia-Hung Hung b, Jeng-Dong Hsu c, Mon-Yuan Yang b, Shing-Jung Wang d,Chau-Jong Wang b, Journal of Cereal Science 53 (2011) 73-77.

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2.2.27 Chemoprevention of 1,2-Dimethylhydrazine-induced Colon Cancer inMice by Naturally Occurring Organosulfur Compounds.

Hiromichi Sumiyoshi and Michael J. Wargovich, CANCER RESEARCH 50. 5084-5087, August 15,

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1990.

2.2.28Protective role of luteolin in 1,2-dimethylhydrazine induced experimentalcolon carcinogenesis.

V. Manju and N. Nalini, Cell Biochemistry And Function.

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2.2.29 Enhancement of Rat Colon Carcinogenesis by Wheat Bran Consumptionduring the Stage of 1,2-Dimethylhydrazine Administration.

Lucien R. Jacobs, Cancer Res. 1983; 43:4057-4061.

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III. PUBLISHED REVIEW ARTICLES

3.1 Anti-Cancer Activity of Cow Urine: Current Status and Future Directions

K.Dharma R.S.Chauhan and lokesh Singhal, International Journal of Cow Science,1-25

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3.2 Is there a Role for Herbal Medicine in the Treatment of Pancreatic Cancer?

Muhammad Wasif Saif, JOP. J Pancreas (Online) 2008; 9(4):403-407

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3.3Natural compounds for cancer treatment and prevention.

Stefania Nobili , Donatella Lippi , Ewa Witort , Martino Donnini, Letizia Bausi Enrico Mini ,Sergio Capaccioli. Pharmacological Research 59 (2009) 365378

54

3.4Natural Herbs as Anticancer Drugs.

Nidhi Agarwal ,Chandana Majee,G. S. Chakraborthy, International Journal of PharmTechResearch, Vol.4, No.3, pp 1142-1153, 2012.

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3.5 Traditionally Used Anticancer Herbs In India.

M. Umadevi, K.P.Sampath Kumar, Debjit Bhowmik, S. Duraivel. Journal of Medicinal PlantsStudies, 2013, Vol 1, (3) ; 56 -74.

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3.6 A review of traditional anticancer nano-medicine: triphala.

Amit Kumar, The Pharma Innovation Journal 2014; 3(7): 60-66.

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3.7 Ayurvedic medicine in treatment of Cancer.

Sunyana Jain, Vikrant Gill, Neeru Vasudeva, Neelam Singla, Journal of Chinese integrativeMedicine, Nov. 2009, Vol. 7, No.11, 1096- 1099.

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3.8 Canceran integrative approach by Ayurveda.

Kulkarni Anand R, Reddy RG, Marlewar SS, Wadikar SS, Jangle VM, Kulkarni Amruta P.,International Journal of Experimental Pharmacology, Vol 3 | Issue 2 | 2014 | 47-51.

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3.9 Canceran ayurvedic perspective.

Premalatha Balachandrana, Rajgopal Govindarajan, Pharmacological Research 51 (2005) 1930.

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3.10 Canceran Ayurvedic Perspective

Dipal Patel, AhamedNoor Mansoori, IJARPB, 2012; Vol.2 (2):179-195.

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3.11 Medicinal Plants of Asian Origin Having Anticancer Potential: ShortReview.

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Sushma Kainsa, Praveen Kumar, Poonam Rani, Asian Journal of Biomedical and PharmaceuticalSciences, 2(10) 2012, 01-07.

3.12 Therapeutic Potential of Ocimum sanctum in Prevention and Treatment ofCancer and Exposure to Radiation: An Overview.

N. Singh, P. Verma, B. R. Pandey, M. Bhalla, International Journal of Pharmaceutical Sciences andDrug Research 2012; 4(2): 97-104.

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3.13 From Traditional Ayurvedic Medicine to Modern Medicine:Identificationof Therapeutic Targets for Suppression of Inflammation and Cancer

Bharat B.Aggrawal, Haruyo Ichikawa, Prachi Garodia, Priya Weerasinghe, Gautam Sethi, Indira DBhatt, Manoj Pandey,Shishir Shishodia,Muraleedharan G Nair, Expert Opinion Ashleypublications ,2006,page no.87-117

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CONCEPT PAPER


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Concept NoteAYUSH in Cancer Care

Cancer is most dreaded disease of the 21st Century and spreading further with continuance andincreasing incidence. Multidisciplinary scientific investigations are making best effort to combatthis disease, but the cure for the disease is still eluding. With the advent of gene therapy somehopes are there for future management of the disease. Management of cancer includes surgery,radiation therapy, chemotherapy and biological therapy resulting in cure of >50%patientsdiagnosed with cancer. The modern approach to the treatment of cancer has reached the plateau.

Owing to the importance/benefits of traditional medicine WHO included integrative oncology asone of the primary objective of treatment of cancer besides cure/ prevention of recurrence,prolongation of life& rehabilitation and improvement of quality of life. Sushruta known as father ofsurgery described the disease as a swelling situated either superficially or in deeper structure inrelation to different tissues based entirely on clinical manifestations, course, prognosis andtreatment available in those periods. However, while going through the different ancient literature ithas been observed that there are various types of diseases viz. Apachior Apachit (multiple lymphnode swelling), Arbuda (tumour), Gulma (lumps especially ih inner organs), Granthi (Cyst),Asadhya Vrana (non-healing ulcers) etc. described in Ayurveda which closely relate to the presentnomenclature of cancer.Arbudacan be characterized as fleshy elevated swelling, round and fixedmass, sometimes deep seated, large and non-suppurating, Occasionally painful or painless swelling,occurring anywhere over the body with predominance ofKapha DosainvolvingMamsa Dhatu.

Treatment ofArbudahas been described elaborately by different Ayurvedic physicians. Caraka hasmentioned the treatment ofArbudaalongwith the treatment of localized Shopha (Granthi) Most ofthe Rasayana drugs especially which are Balya (tonic), Brimahana (nutritive), Shramahara (anti-fatigue) and Jeevaniya (nourishing, anti-oxidant common immunomodulation) will be useful in themanagement of these conditions.

The management according to Ayurveda includes Systemic management comprising ofSamshodhan Chikitsa (purifactory), Shaman Chikitsa (palliative), Rasayan Chikitsa (rejuvenative)and Local treatmentviz. Alep (anointing), Parisheka (medicated spray), Kshara Karma(causticapplication) etc. Some medicinal plants such as kancanar (Bauhinia variegata), Lajjalu (Mimosapudica), Karveera (Nerium indicum), Kushtha (Sassurea lappa), Bhallataka (Semecarpusanacardium), Haritaki (Terminalia chebula), Peet-karaveera (Thevetia peruviana), Sadapuspi(Vinca rosea), Neelapushpa (Viola odorata), Rasona (Allium sativum), Ghritkumari (Aloe vera),Saptaparna (Alstonia scholaris), Haridra (Curcuma longa), Vrischikali (Heliotropium indicum),Shigru (Moringa oleifera ), Krishna Jeeraka (Nigella sativa), Tulsi (Ocimum sanctum), Talishapatra (Taxus buccata ), Ashwagandha (Withania somnifera ) ,Sitaphala (Annona squamosa)etchave been mentioned in various text of Ayurveda for the management of such diseases.

Scientific evidences: Ayurvedic interventions as add-on therapy to conventional therapies haveproven with some advantages in cancer management such as:

Effect of Guduchyadi Yoga (containing Guduchi, Amalaki, Ashwagandha, Yastimadhu,Jivanti, Tulasi, Pippali) in Avaleha form in the dosage of 15 gram twice daily along withRadiotherapy and Chemotherapy against adverse effect of radiotherapy & chemotherapy incancer. (Ref. Vyas Poorvi - A clinical study on a Rasayana as a radio-protective and chemo-protectiveadjuvant in the management of carcinoma, M.D.(Ayu) thesis, Gujarat Ayurved University, 2005.)

Rasayana Avaleha containing Mustaka, Usheera, Chandan, Guduchi, Bala, shatavari,ashwagandha, Gokshur, sariva, Manjishtha, Bibhitak, Nirgundi, Munnaka, kapikachhu, Pippali,Shunth, Haridra, Tulasi and sugar and Ghee. was used in the dose of 10 Gm per day for threemonths along with Radiotharapy & chemotherapy before, along and after the completion of the

treatment up to 3 months. The study revealed relief in the complaints of nausea and vomiting


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constipation, mucositis and WBC count was maintained in the group treated with RasayanaAvaleha and chemo therapy when compared to chemo therapy treated group. (Ref. MankadZankhana A clinical study on the role of Rasayana as pre, adjuvant and post treatment ofChemotherapy in the management of Carcinoma, M.D.(Ayu.) thesis, Gujarat Ayurved University, 2007)

Protective role of Yastimadhu against side effects of Radiation/Chemotherapy in cases of headand Neck malignancies has revealed highly significant relief on mucositis and changing voicein Group A& B as compared to the Control. (Ref. Das Debabrata Nirmalendu - A clinical trial on

protective role of Yashtimadhu (Glycyrrhiza Glabra) against side effects of radiation/chemotherapy incases of head & neck malignancies, M.D.(Ayu) thesis, Gujarat Ayurved University, 2010)

Atharva Anant Kalpa (Hemidesmus indicus) preparation in the cases of cancer subjected tochemotherapy in the dose of 5 gram b.i.d. during and after chemotherapy and radiotherapyminimum for three months showed that the drug is found to be effective in the minimizing oftoxicities of chemotherapy and radiotherapy. nausea, loss of appetite, vomiting, diarrhea wereremarkably reduced in more than 60% patients. Maintenance of weight & general wellbeingwas possible as food intake was not hampered, which is commonly observed during the courseof Radiotherapy. (Ref. S P Sardeshmukh & Vasanti Godse - Management of side effects of cancerchemotherapy with Ananata Kalpa a sugar base Ayurvedic preparation of Ananta (Hemidesmus indicus),selected research papers published in National Seminar on Management of Cancer through Ayurveda

by R.A.V. , New Delhi, 6-7 Feb. 2012 and S P Sardeshmukh & Shweta Gujar Efficacy of AnantaKalpa in reducing side effects of Radiotherapy in oral cancers, selected research papers published in

National Seminar on Management of Cancer through Ayurveda by R.A.V. , New Delhi, 6-7 Feb.

2012.) A polyherbal Ayurvedic drug Indukanta Ghritha as a adjuvant to cancer chemotherapy via

immunomodulation. Laboratory of Tumor Immunology and funtional Genomics Preliminarystudies in lab showed immunomodulatory effects with a Th1 type of immune response.Inhibittumor development in Mice challenged with Daltons Lymphoma ascites, InducedLeucopoiesis, Enhanced Median survival time as well as life span in tumor bearing animals.Elevated Macrophage phagocytic activity, Reverse cyclophosphamide inducedmyelosuppression in control tumor bearing animals to values near normal levels. Thus theeffect of Indukanta gritha in patients suffering from leukopenia was beneficial. (Ref. Suraj KGeorge,Rajesh,Sunil Kumar S,B Sulekha,Prabha Balaram - A polyherbal Ayurvedic drug IndukantaGhritha as a adjuvant to cancer chemotherapy via immunomodulation Laboratory of Tumor Immunologyand Funtional Genomics,Division of cancer Research, Regional Cancer Center,Trivandrum,India)

CCRAS research contribution on Cancer: CCRAS has conducted some experimental andclinical studies as under

Screening of medicinal plants on Anticancer Activity: In vitro Screening of 14 StandardizedPlant Extracts Ayurvedic herbs for anti-cancer activity was carried out through ACTREC,Mumbai on various cell lines using taking leads from Ayurvedic Classical Literature. The studyrevealed anti cancer activity of 9 plants extracts viz. Berberis aristata, Piper longum, Picrorhizakurroa,Cedrus deodara, Withania somnifera, Phyllanthus embelia, Acorus calamus, Bauhiniavariegata, Terminalia chebula against cancer cell lines. (Report on Screening of single Herbaldrug extracts for potential anti-cancer activity; 2009, Central Council for Research Ayurveda &Siddha, New Delhi.)

Clinical study on certain indigenous drug for the management of cancer :After primaryscreening of number of indigenous drugs i.e. Bhallatak (Semecarpus anacardium, Rohitak(Amoora rohitak), Madhuyasti (Glycyrrhiza Glabra), Karvir, (Nerium odorum), Gunja (Abrusprecatorius), Gugguluetc. on experimental animals few of them were selected for further studyboth in vivo and in vitro. This clinical study was carried out involving 400 cancer cases of eithersex, different types, sites stages for ten years The study concluded that Ayurveda stand alone andas add-on/ adjuvant therapy to radio/chemo therapies have shown Significant increase in Hb%,Prevent fall in Hb%, loss in body weight inhabited, significant gain in weight, increase in GADlevels, increase in survival period, Improved immune status (IgA Levels) when compared toradio/chemo therapies alone. (Ayurvedic drugs in the management of Cancer; 1999, CentralCouncil for Research Ayurveda & Siddha, New Delhi)


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Recent Initiative under taken by the Council in the field of Cancer: Council has developed anAyurvedic coded drug AYUSH-QOL 2C for improvement in quality of life in cancer patients.Prospective double blind placebo controlled clinical trial of AYUSH-QOL 2C as an adjuvant tochemotherapy/ radiotherapy in Breast Cancer and Lung Cancer patients have been undertakenwhich are in progress .


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II. RESEARCH

PUBLICATIONS

2.1 CLINICAL RESEARCH


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International Journal of Ayurveda Research | Jan-Mar 2011| Vol 2| Issue 1

Treatment of relapsed undifferentiated acute myeloid leukemia (AML-M0)with Ayurvedic therapy

Balendu Prakash

V C P Cancer Research Foundation (Scientific and Industrial Research Organization), MandirMarg, Turner Road, Clement Town, Dehradun, India

ABSTRACTA 16-year-old boy was detected with acute myeloid leukemia (AML M0) with bone marrow

pathology showing 85% blasts in February 07, 1997. He received two cycles of inductionchemotherapy (3+7 protocol) with daunomycin and cytosar, following which he achievedincomplete remission with bone marrow aspirate showing 14% blasts. Subsequently, the patient

received two cycles of high-dose cytosine arabinoside Ara-C and achieved remission. However,his disease relapsed on August 29, 1997. Peripheral blood smear showed 6% blast cells and bonemarrow showed 40% blast cells. The patient refused further chemotherapy and/or bone marrowtransplant and volunteered for Ayurvedic therapy (AYT) advocated by the author fromSeptember 09, 1997. Bone marrow studies done after six months of AYT indicated that thedisease was in remission. The AYT was continued for five years and stopped. Thereafter, the

patient received intermittent maintenance AYT for three months in the next two years. Atpresent, the patient is normal and healthy and has completed 12 years of disease-free survivalwith AYT.

Key words:Ayurvedic, relapsed acute myeloid leukemia


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Journal of Ayurveda Vol.II No.4 Oct-Dec 2008

Taming the side effects of radiotherapy in oral carcinoma patients with help ofAyurvedic Medicine (Clinical Study)

*Dr Sunil Gupta, **Dr H. K. Kushwaha, ***Dr Amita Jhunjhunwala, ****Dr D.P.Agarwal

*Ph.D Scholar Shalya,NIA, **Prof.&H.O.D. Shalya tantra NIA Jaipur, ***Ph.D Scholar RasaShastra & Bhaishajyakalpana,NIA**** Prof. &H.O.D.Radiotherapy unit S.M.S. Hospital Jaipur

Abstract-The role of an ayurvedic formulation is evaluated to minimize the side effects of radiotherapysufferingfrom oral carcinoma in this study. 30 patients of oral carcinoma were taken for this study and

divided in three groups of 10patients in each group. Comparative study was donebetween threegroupsby givingradiotherapy alone in one group and ayurvedic formulation + Radiotherapy inother two groups at different intervals. Results suggested that there was significant reduction ofthe side effects in the patients who weretaking Ayurvedic formulation along with Radiotherapy.

Keywords - Oral carcinoma, Side effects, radiotherapy, Formulation, brachytherapy, mucositis,anorexia and salivary reactions.


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Journal of Ayurveda Vol.II No.4 Oct-Dec 2008

Effect of intervention of Ayurveda & Practical implementation of Shadchakras

(PranicHealing) An indigenousapproach as co-therapywith

Chemotherapy&Radiotherapy for physical & mental wellbeing of cancerpatientsA Clinical Study

*Dr.Pooja Sabharwal, **Dr.M. Dinkar Sarma,***Dr.D. P.Agarwral

Cancer a major killer, the most dreaded among diseases, is fast engulfing the g1obe posingthe challenge the ingenuity of human beings. Patients who present with cancer have basicoptions for treatment. The first and the most conventional treatments utilize chemotherapy,surgery and radiation. Second are a wide range of alternative therapies. Third is a

combined approach. Conventiona1 treatments for cancer have varying success rates.Statistics are available for the success and failure of treatments. A list of side effects for eachtreatment is also available. There has been considerably adverse effects like nausea, itching,emesis, fatigue and ultimately it deteriorated quality of life on physical, functional, social and

psychological domains.

Hypothesis: Thus the present study was designed to evaluate the effects of herbal compoundoncocare capsule and pranic healing along with radiotherapy in cancer patients.

Materials & methods: The study was conducted in 8o patients who were suffering from ca n ce r .Four groups were made. First was control group; oncocare capsule was given in second group, third

group receives only pranic healing techniques and group four receives both oncocare and pranic

healing techniques. Patients were assessed on the basis of subjective and objective, biochemical and

psychological parameters.

Results: There was highly significant improvement in fourth group that is combination ofherbalformulation along with stress management techniques.

Keywords: Cancer, shadchakras, pranic healing, Ayurveda.

*M.D (Ayu),NIA,Jaipur **Prof.&H.O.D. Sharir Rachna,NIA Jaipur, *** Chief RadiationOncologist, S.M.S. Hospital Jaipur


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International Research Journal of Pharmacy 2012, 3(7)

AN EFFECTIVE HERBOMINERAL TREATMENT AS AN ADJUVANT THERAPY FOR THEIMPROVEMENT OF QUALITY OF LIFE OF HEPATOCELLULAR CARCINOMA (HCC)

PATIENT: A CASE REPORT

Jayawardhane N.D.N.1*,De Silva R.H.S.K.2, TripathiJ.S.3, Narasimha Murthy K.H.H.V.S.S.4,Tiwari S.K.5Department of Kayachikitsa, Institute of Medical Sciences, Banaras Hindu University, Varanasi, India

Article Received on: 01/05/12 Revised on: 16/06/12 Approved for publication: 02/07/12*Email:[email protected]

ABSTRACTAccording to WHO reports Hepatocellular carcinoma remains an Asian health problem. Its

prevalence disproportionately shares large of the world's nearly 78%. Treatment options of HCC arelimited and the effectiveness of treatment varies due to development of therapy-related adverse

effects in Allopathic medicine. We report herein a case of HCC admitted to Sir Sundarlal Hospital,Banaras Hindu University, Varanasi was treated with integrated Ayurvedic herbomineral medicine,with desirable results of improvement in QoL. The patient was treated with a holistic interdisciplinary approach i.e., Modern medicine treatment followed by Ayurveda, health education forcancer care and psychotherapeutic measures such as Yoga, psychological counseling etc. Thesymptoms were managed according to its clinical presentation and daily clinical evaluation. This

paper demonstrates the findings of our experience in treating a case of HCC with Ayurvedic herbo-mineral medicine as an adjuvant treatment for improvement of QoL. Moreover, it emphasized theneeds to be explored Ayurvedic cancer management with more advance methodology.

Key Words: Ayurveda, Hepatocellular carcinoma, Herbomineral treatment, QoL
mailto:[email protected]:[email protected]:[email protected]:[email protected]


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AYU| Apr-Jun 2011| Vol 32| Issue 2

Protective effect of Yashtimadhu (Glycyrrhiza glabra) against side effects ofradiation/ chemotherapy in head and neck malignancies.

Debabrata Das1

, S. K. Agarwal2

, H. M. Chandola3

1. M.D.(Ayu.), Speciality: Kayachikitsa, Medical Officer, Shilong, 2. Professor and Head, Department ofRadiotherapy, M.P. Shah Medical College and G.G. Hospital, Jamnagar, 3. Professor and Head,Department of Kayachikitsa, Institute for Post Graduate Teaching and Research in Ayurveda, GujaratAyurved University, Jamnagar, Gujarat, India.

ABSTRACTOne of the very common side effects of Radiation/Chemotherapy especially of the head andneck malignancies is mucositis. Cancer therapy or the cancer itself may cause changes inthe body chemistry that results in loss of appetite, pain, nausea, vomiting, diarrhoea and very

common mucositis which makes eating difficult. Loss of appetite is followed by an undesirableloss of weight due to insufficient amount of calories every day which can lead to loss of musclemass and strength and other complications by causing interruptions of medical therapy, impedingeffective cancer therapy. Mucositis cause decreased immunity and quality of life as well as poortolerance to surgery and altered efficacy of Chemotherapy and Radiotherapy.

The present study is designed with the objective to minimize the radiation induced mucositis,skin reaction, xerostomia, change in voice etc. with an Ayurvedic preparation YashtimadhuGhrita (processed ghee). Total 75 patients were randomly divided into four groups and drugswere administered: Group A with local application of Yashtimadhupowder and honey in theoral cavity for few minutes prior to radiotherapy along with oral intake of Yashtimadhu Ghrita;

Group B with only local application of the Yashtimadhupowder and honey in the oral cavity;Group C patients administered with only local application of honey in the oral cavity; Group Don conventional modern medication controlled group. All these patients under four groups hadreceived Radiotherapy and Chemotherapy for maximum duration of 7 weeks. Mucositis and Skinreactions were observed in 100% of patients with varying degree. The intensity of Radiation andChemotherapy induced mucositis was reduced to a great extent by the trial drug. Yashtimadhu(Glycyrrhiza glabra) can be used effectively in prevention and treatment of oral mucositis postradiation and chemotheraphy in patients of cancer, especially of the head and neck region. It

proves beneficial in two ways: (i) there were no interruptions in the treatment, and (ii) foodintake was not severely affected leading to maintenance of nutritional status of the patients.

Key words: Head and neck cancer, oral mucositis, radio-chemotherapy, Yashtimadhu ghrita


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AYU| Oct-Dec 2010| Vol 31| Issue 4

Efficacy of Rasayana Avaleha as adjuvant to radiotherapy and chemotherapy inreducing adverse effects

Purvi Vyas1, A. B. Thakar2, M. S. Baghel3, Arvind Sisodia4, Yogesh Deole5

1Kshara Sutra Vaidya -Class 1, Smt.Maniben M.A.H. Government Ayurvedic Hospital, Asarava, Ahmedabad,2Reader, Department of Panchakarma, Institute for Post Graduate Teaching and Research in Ayurveda, GujaratAyurved University, Jamnagar, 3Director, Institute for Post Graduate Teaching and Research in Ayurveda,Gujarat Ayurved University, Jamnagar, 4Professor & Head of Cobalt Unit, Govt. Medical College, Bhavnagar,5Ph.D.Scholar, Department of Panchakarma, Institute for Post Graduate Teaching and Research in Ayurveda,Gujarat Ayurved University, Jamnagar, Gujarat, India.

ABSTRACTCancer is the most dreadful disease affecting mankind. The available treatments such as chemotherapyand radiotherapy have cytotoxic effects, which are hazardous to the normal cells of the patient, causingmany unnecessary effects. This further leads to complications of the therapy, impaired health, anddeterioration of quality of life, resulting in mandatory stoppage of the treatment. In the present study,the efficacy of an Ayurvedic formulation,Rasayana Avaleha, has been evaluated as an adjuvantmedication to modern radiotherapy and chemotherapy. A total of 36 cancer patients were registered inthis trial and were divided into two groups, group A and group B. In group A, the patients were treatedwith radiotherapy and chemotherapy along with adjuvantRasayana Avaleha(RT + CT + RA), while ingroup B only radiotherapy and chemotherapy (RT + CT) were given, as the control group. Afterassessing the results, it was observed thatRasayana Avaleha gave better results in controlling the

adverse effect of chemotherapy and radiotherapy in comparison with the control group. Therefore,Rasayana Avaleha has proved to be an effective adjuvant therapy in protecting patients from theadverse effects of chemotherapy and radiotherapy.

Key words: Cancer, Radiotherapy, Chemotherapy, Adverse Effects, Ayurveda,Rasayana Avaleha


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Efficacy of Varunadi Ghritha (polyherbal compound) in treated head and neckcancer cases as a biological response modifier

Divya Ravindran, Indhu Hariharan 1, Richard Muwonge2, Rejnish R. Kumar, M.Radhakrishna Pillai1, Kunnambath Ramadas

Department of Radiation Oncology, Regional Cancer Centre, Thiruvananthapuram, 1IntegratedCancer Research Program, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala,India, 2Early Detection and Prevention Section, International Agency for Research on Cancer, Lyon,France.

ABSTRACTBackground: Persistent immune suppression is reported in Head and Neck Cancers (HNC) evenafter treatment and a higher recurrence rate was observed in patients with poor CD3 count. Locoregional recurrences and second primary tumours are the common forms of failure in head and neck

cancers. Several agents have been tried to overcome this problem without much benefit. In Ayurveda,several plant based products have been reported to have anti-tumour and immunomodulatoryproperties.Aim: To test the role of Varunadi Ghritha,as an immunomodulator in apparently healthy, treated andcontrolled HNC patients and to evaluate its effectiveness in preventing locoregional relapses anddevelopment of second primary tumours. Materials and Methods: Total 78 patients of treated headand neck cancers were randomly selected for intervention and control group. Patients in theintervention group (n = 38) received Varunadi Ghritha, 5gms twice daily for one year and followedup to two years. Patients in the control group (n = 40) were followed up at regular intervals. Immune

parameters were assessed in the peripheral blood at base line and at the end of administration of thestudy compound. Results: In the intervention group, mean percentage increase in CD3, CD19 and

CD16 positive cells were significantly higher after the administration of the study compoundcompared to the control group indicating an immunomodulatory effect of the study compound. Anon-significant improvement in disease control was observed in patients with advanced stage ofdisease in the intervention group. Conclusion: Administration of Varunadi Ghritha resulted in anincrease in T cell counts in patients with treated HNC.

Key words: Ayurveda, head and neck cancer, immunomodulation, loco-regional control, secondPrimary tumour.


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Clinical ResearchLow resource screening method of precancerous lesions and its reversal by

Triphala in teenage Indian population

Anshula Deshpande, Shobha Tandon1, Neeraj Deshpande2

Departments of Pedodontics, K. M. Shah Dental College, Sumandeep Vidyapeeth, Vadodara,Gujarat, 1Department of Pedodontics, Babu Banarasi Das College of Dental Sciences, BBDUniversity, Lucknow, Uttar Pradesh, 2Periodontics, K. M. Shah Dental College, SumandeepVidyapeeth, Vadodara,Gujarat, India.

AbstractABSTRACT

Background: Cancer screening is the main weapon for early detection at a pre-invasive orpremalignant stage. It has been reported that over 12 million people use some form of tobacco,which is one of the high risk factors and has hence become an alarming world-wide problem.Aim: To evaluate the effective diagnostic screening of disease in its early stage by inexpensivemethod and also to evaluate the effect of indigenous mouth rinse on reversal of pre-cancerouslesions. Materials and Methods: The screening for teenagers belonging to low socio-economicstatus was carried out. Suspected subjects were evaluated for the reversal of the lesions by use ofAyurvedic preparation as a mouthwash. From 13 to19 years working-child population of North Indiawas selected for the study. Screening was performed by new method-visual inspection with aceticacid. The positive subjects were further investigated by pap smear and biopsy was done as aconfirmatory histopathological report. In second phase, the subjects showing positive lesions were

advised indigenous anti-cancer mouth rinse and its effect was evaluated after 6 month and 9 monthof prescribing the rinse. Results: The total 1095 children were screened (831 boys and 264 girls).Out of total 34 teenager boys were diagnosed, as acetowhite positive lesion. All the acetowhite

positive lesions were found exclusively in males. Histological findings after 9 month use of Triphalamouth rinse revealed no changes in cells in 23 (85.2%), hyperkeratinization in 2 (7.4%),hyperkeratinization and spongiosis was evident in 1 (3.7%), mild pleomorphism in 1 (3.7%) patient.Comparative evaluation from 0-9 month showed statistically highly significant test (P < 0.01).

Conclusion: Use of different forms of tobacco and betel nut showed convincing relationshipbetween developments of oral pre-cancerous lesions. Triphala was found to have great potential forreversal of these lesions.

Key words: Alcohol, oral pre-cancerous lesions, screening, tobacco, Triphala and teenagers.


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AYU| Jul- Sep 2012| Vol 33| Issue 3

Swarna Bhasma in cancer: A prospective clinical study

Soumen Das, Mangal C. Das1

, Retina Paul2

Assistant Professor, Department of Surgery, Institute of Post Graduate Medical Education andResearch (SSKM Hospital), 1Director, Department of Integrated Medicine, The CalcuttaGastroenterology Research Institute, Kolkata, West Bengal, 2Resident, Department of Microbiology,Padmashree Dr. D.Y. Patil Medical College, Pune, Maharashtra, India

ABSTRACTDespite the advances in the treatment of cancer, mortality is still high. Complementary and alternativemedicine is emerging as a potent modality in cancer treatment. Swarna Bhasma (SB), containing gold

particles, is an ancient Indian medicine has shown its anticancer activity. This present study wasconducted to detect the effect of SB on solid malignancies. A total of 43 patients were included in thisstudy received SB for 1 year. Seventeen patients showed response. The response was best in rectalcancer group 70% (7/10). Nearly 41.02% patients survived for 1 year after treatment but after 5 yearsthis came down to 15.38%.

Key words: Cancer, complementary medicine, gold particle, Swarna Bhasma


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Journal of Ayurveda Vol.1No.1 Jan-Mar 2007

Evaluation of Clinical Response of Carcinolyt (Herbal Nutrients) To ControlAdverse Effects of Radiotherapy in Cancer Patients.

* Dr . Amanpreet Kaur Broca, **Dr . Umesh Chandra Sharma, ** *Prof Hetnant Kumar Kushwaha* * * * Prof S. S. Sharma * * * * * Dr. D.P. Agarwal * * * * * * Dr. Arun Chauguley

*Incharge, Shalya Tatra Unit, GAH,Jammu**Lecturer,Shalya Vibhag,GuruNanak Dev Medical College, Ludhiana***Professor &Head, Dept. of Shalya Tantra,N.I.A,Jaipur****Professor & Ex-Head, Dept. of Shalya Tantra,N.I.A. Jaipur*****Professor & Head, Radiotherapy unit S.M.S. Hospital,Jaipur******Associate Professor, Radiotherapy unit S.M.S. Hospital, Jaipur

ABSTRACTThe Best in the cancer treatment will come through a combination of conventional and alternative

medicine. Ayurveda can play a vital role in palliative, promotive and preventive strategy againstcancer. The importance and utility of Ayurveda in cancer management is because there are limitationsto the present treatment modalities of this fatal disease, which are well known for their toxic effects andcomplications. In thepresent study, an indigenous formulation- Carsinolyt (awaleha & Ghan Satvaa)was evaluated to prevent side effects of Radiotherapy Trial was done on forty patients, Group APatients were given carsinolyt and radiotherapy simultaneously whereas Group B patients were givenradiotherapy only.

The results achieved were encouraging with improvement of 56.62% in mucosal reactions, 37.5% inHaematological status, 18.18% in Pain, 8.33% in Salivary reactions, and 21.42% in Skin reactions.Carsinolyt incorporates Agnideepak, Amadoshahar, Vrana shodhak, Vrana ropak, Vedna sthapak,

Vishaghna, Gandamala Nashak, Mukh shodhak, Raktashodhak & Balya drugs and the reduction ofcytotoxic effects of radiotherapy also owe to the same actions. Carsinolyt proved to be non-toxic,immunomodulator, adaptogenic and radioprotectivepreparation.

Key words: Herbal, Radioprotective, Cancer, Radiotherapy, V1'Qnashodhak,Rakta Shodhak, Agnideepak;Balya,Cytotoxic.


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Journal of Clinical Oncology 2014 DOI: 10.1200/JCO.2013.51.8860

Yogas Impact on Inflammation, Mood, and Fatigue in Breast CancerSurvivors: A Randomized Controlled Trial

Janice K. Kiecolt-Glaser, Jeanette M. Bennett, Rebecca Andridge, Juan Peng, Charles L. Shapiro,William B. Malarkey, Charles F. Emery, Rachel Layman, Ewa E. Mrozek, and Ronald GlaserInstitute of Behavioral Medicine Research, The Ohio State University College of Medicine,Columbus, OH43210

A B S T R A C T

PurposeTo evaluate yogas impact on inflammation, mood, and fatigue.

Patients and Methods

A randomized controlled 3-month trial was conducted with two post-treatment assessments of 200 breastcancer survivors assigned to either 12 weeks of 90-minute twice per week hatha yoga classes or a wait-listcontrol. The main outcome measures were lipopolysaccharide-stimulated production of proinflammatorycytokines interleukin-6 (IL-6), tumor necrosis factor alpha (TNF-_), and interleukin-1_ (IL-1_), and scoreson the Multidimensional Fatigue Symptom Inventory-Short Form (MFSI-SF), the vitality scale from theMedical Outcomes Study 36-item Short Form (SF-36), and the Center for Epidemiological Studies-Depression (CES-D) scale.

ResultsImmediately post-treatment, fatigue was not lower (P_ .05) but vitality was higher (P _ .01) in the yogagroup compared with the control group. At 3 months post-treatment, fatigue was lower in the yoga group(P_ .002), vitality was higher (P_ .01), and IL-6 (P_ .027), TNF-_ (P_ .027), and IL-1_ (P_ .037) were

lower for yoga participants compared with the control group. Groups did not differ on depression at eithertime (P _ .2). Planned secondary analyses showed that the frequency of yoga practice had strongerassociations with fatigue at both post-treatment visits (P_ .019;P_ .001), as well as vitality (P_ .016;P_.0045), but not depression (P_ .05) than simple group assignment; more frequent practice produced largerchanges. At 3 months post-treatment, increasing yoga practice also led to a decrease in IL-6 (P_ .01) andIL-1_ (P_ .03) production but not in TNF-_ production (P_ .05).

ConclusionChronic inflammation may fuel declines in physical function leading to frailty and disability. If yogadampens or limits both fatigue and inflammation, then regular practice could have substantial healthbenefits.


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Explore (NY).2013; 9(4):232-243. Doi:10.1016/j.explore.2013.04.005

Effects of Yoga Interventions on Fatigue in Cancer Patients andSurvivors: A Systematic Review of Randomized Controlled Trials

Julie Sadja, MS1,2

and Paul J. Mills, PhD2

1 San Diego State University (SDSU) & University of California, San Diego (UCSD), Joint DoctoralProgram in Clinical Psychology, San Diego, CA

2 Department of Psychiatry, Behavioral Medicine Program, UCSD, La Jolla, CA

AbstractBackgroundFatigue is one of the most frequently reported, distressing side effects reported bycancer survivors and often has significant long-term consequences. Research indicates that yoga can

produce invigorating effects on physical and mental energy, and thereby may improve levels of fatigue.

The objective of this systematic review was to examine the literature that reports the effects ofrandomized, controlled yoga interventions on self-reported fatigue in cancer patients and survivors.The online electronic databases, PubMed and PsycINFO, were used to search for peer reviewedresearch articles studying the effects of yoga interventions on fatigue in cancer survivors.Combinations of yoga, cancer, and fatigue-related search terms were entered simultaneously to obtainarticles that included all three elements. Studies were included if they met the following inclusioncriteria: participants were male or female cancer patients or survivors participating in randomized,controlled yoga interventions. The main outcome of interest was change in fatigue from pre- to post-intervention. Interventions of any length were included in the analysis. Risk of bias using the format ofthe Cochrane Collaborations tool for assessing risk of bias was also examined across studies.

Results Ten articles met inclusion criteria and involved a total of 583 participants who werepredominantly female, breast cancer survivors. Four studies indicated that the yoga interventionresulted in significant reductions in self-reported fatigue from pre- to post-intervention. Three of thestudies reported that there were significant reductions of fatigue among participants who attended agreater number of yoga classes. Risk of bias was high for areas of adequate selection, performance,detection, and patient-reported bias and mixed for attrition and reporting bias. Risk of bias wasuniformly low for other forms of bias, including financial conflicts of interest.

ConclusionsResults of the studies included in this review suggest that yoga interventions may bebeneficial for reducing cancer-related fatigue in women with breast cancer; however, conclusions

should be interpreted with caution as a result of levels of bias and inconsistent methods used acrossstudies. More well-constructed randomized controlled trials are needed to determine the impact of yogainterventions on fatigue in cancer patients and survivors.

KeywordsYoga; cancer; fatigue


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BMC Cancer 2011, 11:19

Classical homeopathy in the treatment of cancer patients - a prospectiveobservational study of two independent cohorts

Matthias Rostock1,4*, Johannes Naumann1,2, Corina Guethlin2,5, Lars Guenther2, Hans HBartsch1, Harald Walach3

1Tumour Biology Center at Albert Ludwigs University Freiburg, Germany.2Dept. of Evaluation Research in Complementary Medicine, UniversityHospital Freiburg, Germany. 3Institute for Transcultural Health Studies andSamueli Institute, European Office, Europa Universitt Viadrina, Frankfurt(Oder), Germany. 4Institute of Complementary Medicine, University HospitalZurich, Switzerland. 5Institute for General Practice, Johann Wolfgang GoetheUniversity Frankfurt, Germany.

AbstractBackground: Many cancer patients seek homeopathy as a complementary therapy. It has rarely beenstudied systematically, whether homeopathic care is of benefit for cancer patients.

Methods: We conducted a prospective observational study with cancer patients in two differentlytreated cohorts: one cohort with patients under complementary homeopathic treatment (HG; n = 259),and one cohort with conventionally treated cancer patients (CG; n = 380). For a direct comparison,matched pairs with patients of the same tumour entity and comparable prognosis were to be formed.Main outcome parameter: change of quality of life (FACT-G, FACIT-Sp) after 3 months.Secondary outcome parameters: change of quality of life (FACT-G, FACIT-Sp) after a year, as well asimpairment by fatigue (MFI) and by anxiety and depression (HADS).

Results: HG: FACT-G, or FACIT-Sp, respectively improved statistically significantly in the first threemonths, from 75.6 (SD 14.6) to 81.1 (SD 16.9), or from 32.1 (SD 8.2) to 34.9 (SD 8.32), respectively.After 12 months, a further increase to 84.1 (SD 15.5) or 35.2 (SD 8.6) was found. Fatigue (MFI)

decreased; anxiety and depression (HADS) did not change. CG: FACT-G remained constant in the firstthree months: 75.3 (SD 17.3) at t0, and 76.6 (SD 16.6) at t1. After 12 months, there was a slightincrease to 78.9 (SD 18.1). FACIT-Sp scores improved significantly from t0 (31.0 - SD 8.9) to t1 (32.1- SD 8.9) and declined again after a year (31.6 - SD 9.4). For fatigue, anxiety, and depression, norelevant changes were found. 120 patients of HG and 206 patients of CG met our criteria for matched-

pairs selection. Due to large differences between the two patient populations, however, only 11matched pairs could be formed. This is not sufficient for a comparative study.

Conclusion: In our prospective study, we observed an improvement of quality of life as well as atendency of fatigue symptoms to decrease in cancer patients under complementary homeopathictreatment. It would take considerably larger samples to find matched pairs suitable for comparison inorder to establish a definite causal relation between these effects and homeopathic treatment.BackgroundMany cancer patients use complementary and alternative medicine (CAM) treatments. Homeopathy isone of the most popular CAM modalities for cancer patients in seven out of 14 European countries [1].Homeopathy has traditionally been very popular in India and South America.


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2.2 PHARMACOLOGY,EXPERIMENTAL STUDIES

AND STANDARDIZATION


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List of Plant extracts selected for screening of anticancer activity

S.No. Name of the Plant Botanical Name Extract Used1. Kantakari Solanum xanthocarpum Hydro-alcoholic

(60:40)2. Yashtimadhu Glycyrrhiza glabra Hydro-alcoholic

(60:40)3. Daruharidra Berberis aristata Hydro-alcoholic

(60:40)4. Pippali Piper longum Hydro-alcoholic

(60:40)5. Sunthi Zingiber offcinalis Hydro-alcoholic

(60:40)6. Katuki Picrorhiza kurroa Hydro-alcoholic

(60:40)7. Guduchi Tinospora cordifolia Hydro-alcoholic

(60:40)8. Vidanga Embelia ribes Hydro-alcoholic(60:40)

9. Devadaru Cedrus deodara Hydro-alcoholic(60:40)

10. Ashwagandha Withania somnifera Hydro-alcoholic(60:40)

11. Amalki Phyllanthus emblica Hydro-alcoholic(60:40)

12. Bhumyanmalaki Phyllanthus amarus Hydro-alcoholic(60:40)

13. Vacha Acorus calamus Hydro-alcoholic(60:40)

14. Kanchanar Bauhinia variegate Hydro-alcoholic(60:40)

15. Haritaki Terminalia chebula Hydro-alcoholic(60:40)


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AYURVEDIC DRUGS IN THE MANAGEMENTOF CANCER

Chief InvestigatorG.C.Prasad

A.B.M.S. Ph. D.. M.A.M.S., F.I.A.P., F.A.M.S., F.A.I.M.Prof. & Head

Department of Shalya-ShalakyaInstitute of Medical Sciences,

Banaras Hindu University, Varanasi221005

Central Council for Research in Ayurveda and Siddha(Deptt. Of I.S.M. & H.)

Ministry of Health and Family Welfare (Govt. of India)New Delhi


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SUMMARY

Cancer is considered to be the most dreadful of all the diseases exixting in the world as it is fatalfor life. The mounting mortality rate reaches 7-10 millions per year all over the world. No diseaseexists without the involvement of Tridosha as per Ayurvedic concepts. So is with the tumor. The highmitotic activity and pain may be attributed to vatic predominance, the voluminous growth is attributed

to Shleshmic property and enzymatic interactions indicates Paittic prodominance.A relative studies of various varieties ofArbudaswas made in comparsion to neoplasia.Kaphaja

andMedaja Arbudawere similar to benign types of tumours.Raktaja Arbudawas similar to malignantneoplasia. Mansaj Arbudas showed mixed characters of benign and malignant lesions.Histopathological characters of tumours were analysed and compared to their Ayurvedic correlates.Gulmas of various varieties were studied in patients of different Prakritis. It was found that themazimum number of cases of cancer were of Shleshmic Prakriti. Mamsa Dhatu was found to bemaximally involed. The maximum incidents of cancer was found in the age group 41-60 years. Theimmune status of the cancer present were assessed in the present study (IgA, IgG & IgM) and the IgAlevels were found low. To some patients Poorva Karmawas administered prior to chemotherapy i.e.

the patients were given Triphala Ghrit and Snehana Karma. Such patients showed incresase in IgA(better immune status). In patients receiving Ayurvedic therapy, prior to chemotherapy, the side effectof chemotherapy viz. nausea, loss of body weight, anemia, skin-discolouration, and hair falling weredecreased. Survival period was more than three years in such patients.

After extensive studies on experimental tumour (in vivo and in vitro) an Ayurvedic formulationwas made for the cancer patients. This formulation consisted of

(a)Bhallataka(b)Rohitaka(c)Madhuyashthi(d)Tamra Bhasma

The patients were divided into five groups to assess the effect of the above mentioned formulationIt was observed that the patients who received Ayurvedic drug as an adjuvant theraphy showedmaximum response. A significant improvement was observed in hemoglobin percentage, body weightand span of life. Patients who received only Ayurvedic drug, revealed result comparable tochemotheraphy treated group. The drug was found more effective in leukeamia with spleenomegalylymphoma, squamous cell carcinoma and adenocarnicoma.

As GABA is a synthetic enzyme and glutamate is a degradading enzyme. GABA is decreased andglutamate is increased in cancer patient. This is due to low GAD activity. It was observed in the

present study that GAD activity declines with age, particularly in paients of Kapha Prakriti. ThusGAD may be considered not only a tumour market but also a biochemical parameter of establishingPrakriti.

The probable mode of action of Ayurvedic treatment may be by increasing body resistance andtumor immunity to fight against cancer cells and accelerating enzymatic activity of glutamatedecarboxy in the blood by arresting the abnormal tumour cells.


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Indian Journal of Traditional Knowledge Vol.12(4),October 2013,pp.682-687

Evaluation of some plant extracts for standardization and anticancer activity

SN Gaidhani1*, Arjun Singh1, Suman Kumari1, GS Lavekar1, AS Juvekar2, S Sen2& MM Padhi11Central Council for Research in Ayurvedic Sciences, 61-65, Institutional area, Opposite D Block, Janakpuri,

New delhi;2Advance Centre for Treatment, Research and Education in Cancer (ACTREC), Kharger, Navi Mumbai-410

210, India*E-mail:[email protected]

Received 17.11.10, revised 04.07.13

In recent times, the trend in cancer research is shifting towards identifying new medicines from natural resourcesfor management of cancer. Medicinal plants such as Sthauneyaka (Taxus baccata L.) and compoundformulations like Triphalaghrita, Khadirarista, Madhusnuhi rasayana, Maha triphaladya ghrita, Panchatiktaguggulu ghrita are indicated in the Ayurvedic texts for management of cancer/ tumour. The anti-proliferativeactivities of hydro-alcoholic extracts of some standardized plant materials were screened against a panel of 14

human cancer cell lines representing different tissues (lung, pancreas, colon, cervix, oral, bladder, prostate,breast, leukaemia, etc.) through Sulforhodamine-B (SRB) assay. The findings revealed that Cedrus deodara(Roxb.) ex Lamb. and Berberis aristata (Roxb.) ex DC. have maximum anticancer activity against 3 cell lineswhile Withania somnifera Dunal. showed activity against two cell lines. In addition to these, PicrorhizakurroaRoyle ex Benth. and Piper longum L. were found active against only one cell line. These results indicate thepotential of Ayurvedic medicinal herbs as anti-neoplastic agents mentioned in the Ayurvedic texts. However,further studies are needed for evaluating their mechanism of action and to isolate the active anticancercompounds responsible for this activity.

Keywords: Medicinal plants, Standardization, Anticancer activityIPC Int. Cl.8: A61K 36/00, A01D 4/04, A01D 4/34


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Phramacognosy Magazine [Phcog.Mag] Vol.5,Issue 20 (Suppl.), Oct-Dec, 2009 Page 425-429

In-Vitro Anticancer Activity of Standard Extracts Used In Ayurveda

Gaidhani S.N.#*, Lavekar G.S.#, Juvekar A.S.##, Sen S##, Singh Arjun#, Kumari Suman#

#Central Council For Research in Ayurveda & Siddha, Dept. of Ayush, Ministry of Health & F.W.,Janakpuri, New Delhi-58. # #ACTREC, TMC Kharghar, Navi , Mumbai , India

* Author for correspondence : *Assistant Director (Pharmacology), Central Council for Research inAyurveda & Siddha, Dept. of AYUSH, Ministry of Health & Family Welfare, Janakpuri, New delhi-58,+919868349837 (m), E-mail-sudeshgaidhani@ gmail.com

ABSTRACTThe hydro-alcoholic extracts of five Ayurvedic medicinal plants, pericarp of Terminalia chebula,rhizome ofAcorus calamus, stem bark of Bauhinia variegate, whole plant of Phyllanthus amarus, rootof Glycyrrhiza glabrawere evaluated for their anti-proliferative activity on fourteen cancer cell lines.These plant extracts were tested bysulforhodamine-B (SRB) assay for its anti proliferative activity andfour extracts except Glycyrrhiza glabrawere found active against prostrate cancer cell line (DU145. Inaddition to this Terminalia chebulaexhibited activity against leukemia cancer cell line (K562).

Keywords: Standardization, Anticancer, Cell Line, SRB Assay.


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J Biomed Sci and Res.,Vol 2 (4), 2010, 231-235

Screening the Anti-Cancerous Efficacy of Achyranthes aspera Linn. using AnimalModel Swiss Albino Mice

Geetha. P1, Narayanan. K.R2* and A.G. Murugesan11Sri Paramakalyani Centre for Environmental Sciences, Manonamaniam Sundaranar University, Alwarkurichi -627 412. 2Department of Advanced Zoology and Biotechnology, Sri Paramakalyani College, Alwarkurichi 627 412, Tamilnadu, India.

Abstract:Achyranthes aspera is a one of the important traditional medicinal plant. In the present investigationanticancer efficiency ofA. aspera was evaluated in Swiss albino mice after treated with mineral oil. InSwiss albino mice the cancer state was induced by intra-peritonial injection of mineral oil at a dose of 1ml/kg of body weight for 21 days. The tail length of the normal mice was 9.6 cm whereas the micewith metastasize tumor in the head had a tail length of 5.8 cm, metastasize throat cancer mice had 5.9

cm of tail length and the mice with plasma cytoma alone had the tail length of 5.4 cm. The anticancerous activity of A. aspera leaves was tested against mineral oil induced cancer mice.Simultaneously a group of mice was first intra-peritoneally injected with the sub-lethal doses (3 mg/ mland 1.5 mg/ ml) of ether extract for 15 days. After 15 days the extract given mice were treated with 1ml/kg of mineral oil periodically for 21 days. It was found that none of the mice got the symptoms ofcancer. The present work clearly indicates that the ether extract at the concentration of 3 mg/ ml is veryeffective in reducing the cancer symptoms.

Keywords:Anti-Cancer activity, mice, mineral oil, ether extract andAchyranthes aspera.


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Journal of Ethnopharmacology 100(2005) 72-79

Plants as a source of anti-cancer agentsGordon M. Cragg , David J. Newman

Natural Products Branch, Developmental Therapeutics Program, Division of Cancer Treatment andDiagnosis, National Cancer Institute, P.O. Box B, Frederick, MD 21702-1201, USA

Accepted 18 May 2005

Available online 11 July 2005.

Abstract

Plant-derived compounds have been an important source of several clinically useful anti-cancer agents.These include vinblastine, vincristine, the camptothecin derivatives, topotecan and irinotecan,etoposide, derived from epipodophyllotoxin, and paclitaxel (taxol). A number of promising newagents are in clinical development based on selective activity against cancer-related molecular targets,including flavopiridol and combretastin A4 phosphate, while some agents which failed in earlierclinical studies are stimulating renewed interest.

Keywords: Camptothecins; Combretastatins; Flavopiridol; Podophyllotoxins; Taxanes; Vincaalkaloids; Cell cycle target inhibitors


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International Journal of Pharmacy and Pharmaceutical Sciences Vol.6, Suppl 1, 2014

PREPARATION AND STANDARDIZATION STUDIES ON VEERA MEZHUGUA SIDDHA ANTICANCER FORMULATION

P RAJALAKSHMI, C SAVARIRAJ SAGAYAM , P BRINDHA

Centre for Advanced Research in Indian System of medicine (CARISM), SASTRA University,Thanjavur, Tamil Nadu. Email:[email protected]

Received: 23 Nov 2013, Revised and Accepted: 25 Dec 2013

ABSTRACTVeeramezhugu is a Siddha formulation which is often prescribed in cancer therapy. It is a poly herbo-metalic preparation comprising Veeram (Corrosive sublimate), Rasam (Mercury),Pooram (Calomel),

Lingam (Cinnaber), Sudam (Camphor), Sambirani (Benzoin), Perungayam (Asafoetida), Vediuppu(Potassium nitrate), Navacharam (Ammonium chloride), Vengaram (Borax), Nervalam seed (Crotontiglium) and honey. In the present work this anticancer Siddha formulation is studied from process and

product standardization point of view. Physicochemical parameters are determined for the end productas per Siddha Pharmacopoeia. Such kind of standardization studies will contribute in establishingscientifically the merits of Siddha herbo-metalic preparations.

Keywords: Veera mezhugu, Preparation, Purification, Standardization.


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Pharma Science Monitor Vol-2,Issue-2,2011

PHARMACOGNOSTIC, PRELIMINARY PHYTOCHEMICALSTUDIES AND ANTICANCEROUS POTENTIAL OF TRIGONELLA

FOENUM-GRAECUM

G. Chauhan*1, M. Sharma1, H. Kharkwal2and A. Varma1

1 Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Block 'E-3', Fourth Floor,Sector 125, Noida, UP 201 303, India.2 Amity Institute of Herbal Research and Studies, Amity University Uttar Pradesh, Block 'E-3', FourthFloor, Sector 125, Noida, UP 201 303, India.

ABSTRACTThe present study deals with the pharmacognostic, preliminary phytochemical studies and anticancerproperties of seeds of Trigonella foenum-graecum. The present paper highlights the macroscopiccharacters of seeds, physico-chemical evaluation, preliminary phytochemical studies and anticancer

properties of the seeds. These observations would be of immense value in the botanical identificationand standardization of the drug in crude form and would help distinguish the drug from its otherspecies. Phytochemical standardization parameters such as moisture content, total ash, water solubleand acid insoluble ash, alcohol soluble and water soluble extractives were determined. Preliminaryidentification of phyto-constituents was performed. HPLC study of the alcoholic extract obtained fromthe seeds was carried out and seven compounds were separated. A comprehensive overview of the

pharmacognostic, phyto-chemical analysis of the seed extract and the literature survey carried out for

the anticancer properties of fenugreek seeds.

Keywords: Medicinal plant, Trigonella foenum-graecum, Fenugreek, Physico-chemical studies,Phytochemical Studies.


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Asian Pacific Journal of Cancer Prevention, Vol 12, 2011

Comparative Anticancer Potential of Clove (Syzygium aromaticum) - an IndianSpice - Against Cancer Cell Lines of Various Anatomical Origin

Vinay Dwivedi1&, Richa Shrivastava1&, Showket Hussain1, Chaiti Ganguly2Mausumi Bharadwaj1*

1Div. of Molecular Genetics and Biochemistry, Inst. of Cytology & Preventive Oncology, Noida, 2Deptof Biotechnology, IILM Academy, Gr. Noida, India

AbstractSpices, active ingredients of Indian cooking, may play important roles in prevention and treatment ofvarious cancers. The objective of the present study is to compare the in vitro anticancer activities ofthree different extracts of Clove (Syzygium aromaticum L), a commonly used spice and food flavouring

agent, against different kinds of cancer cell lines of various anatomical derivations. Water, ethanol andoil extracts were screened for anti proliferative activity against HeLa (cervical cancer), MCF-7 (ER +ve) and MDA-MB-231 (ERve) breast cancer, DU-145 prostate cancer and TE-13 esophageal cancercell lines, along with normal human peripheral blood lymphocytes. Inhibition of cell proliferation wasassessed using MTT assay as a vital stain. In the examined five cancer cell lines, the extracts showeddifferent patterns of cell growth inhibition activity, with the oil extract having maximal cytotoxicactivity. Morphological analysis and DAPI staining showed cytotoxicity to be a result of cell disruptionwith subsequent membrane rupture. Maximum cell death and apoptotic cell demise occurred in TE-13cells within 24 hours by clove oil at 300l/ml with 80% cell death whereas DU-145 cells showedminimal cell death. At the same time, no significant cytotoxicity was found in human PBMCs at thesame dose.

Keywords: Clove - eugenol - GLC - cytotoxicity - DAPI - apoptosis


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Asian Pacific Journal of Cancer Prevention, Vol 13, 2012

Cytotoxicity, Toxicity, and Anticancer Activity of Zingiber Off icinale RoscoeAgainst Cholangiocarcinoma

Tullayakorn Plengsuriyakarn1, Vithoon Viyanant1, Veerachai Eursitthichai1, Smarn Tesana2,Wanna Chaijaroenkul1, Arunporn Itharat3, Kesara Na- Bangchang1*

1Thailand Excellence Center in Drug Discovery and Development, 3Applied Thai TraditionalMedicine Center, Faculty of Medicine, Thammasat University, Pathumthani, 2Department ofParasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand

AbstractCholangiocarcinoma (CCA) is an uncommon adenocarcinoma which arises from the epithelial cells ofthe bile ducts. The aim of the study was to investigate the cytotoxicity, toxicity, and anticancer activityof a crude ethanolic extract of ginger (Zingiber officinale Roscoe) against CCA. Cytotoxic activityagainst a CCA cell line (CL-6) was assessed by calcein-AM and Hoechst 33342 assays and anti-oxidant activity was evaluated using the DPPH assay. Investigation of apoptotic activity was

performed by DNA fragmentation assay and induction of genes that may be involved in the resistanceof CCA to anticancer drugs (MDR1, MRP1, MRP2, and MRP3) was examined by real-time PCR. Toinvestigate anti-CCA activity in vivo, a total of 80 OV and nitrosamine (OV/ DMN)-induced CCAhamsters were fed with the ginger extract at doses of 1000, 3000, and 5000 mg/kg body weight daily orevery alternate day for 30 days. Control groups consisting of 10 hamsters for each group were fed with5-fluorouracil (positive control) or distilled water (untreated control). Median IC50 (concentration that

inhibits cell growth by 50%) values for cytotoxicity and anti-oxidant activities of the crude ethanolicextract of ginger were 10.95, 53.15, and 27.86 g/ml, respectively. More than ten DNA fragments werevisualized and up to 7-9 fold up-regulation of MDR1 and MRP3 genes was observed followingexposure to the ethanolic extract of ginger. Acute and subacute toxicity tests indicated absence of anysignificant toxicity at the maximum dose of 5,000 mg/kg body weight given by intragastric gavage.The survival time and survival rate of the CCA-bearing hamsters were significantly prolongedcompared to the control group (median of 54 vs 17 weeks). Results from these in vitro and in vivostudies thus indicate promising anticancer activity of the crude ethanolic extract of ginger against CCAwith the absence of any significant toxicity. Moreover, MDR1 and MRP3 may be involved inconferring resistance of CCA to the ginger extract.

Keywords: Cholangiocarcinoma - cytotoxicity - ginger -Zingiber Officinale roscoe


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Cancer Letters 231(2006)206-214

Potential of traditional ayurvedic formulation, Triphala, as a novel anticancer drug

T. Sandhyaa, K.M. Lathikaa, B.N. Pandeyb, K.P. Mishraa,*

a Radiation Biology and Health Sciences Division, Bhabha Atomic Research Centre, Mumbai 400 085, India bDepartment of Radiology, New Jersey Medical School, Newark, NJ 07103, USA

Received 8 December 2004; received in revised form 30 January 2005; accepted 31 January 2005

AbstractThe cytotoxic effects of aqueous extract of Triphala, an ayurvedic formulation, were investigated onhuman breast cancer cell line (MCF-7) and a transplantable mouse thymic lymphoma (barcl-95). The

viability of treated cells was found to decrease with the increasing concentrations of Triphala. On theother hand, treatment of normal breast epithelial cells, MCF-10 F, human peripheral bloodmononuclear cells, mouse liver and spleen cells, with similar concentrations of Triphala did not affecttheir cytotoxicity significantly. The drug treatment was found to induce apoptosis in MCF-7 and barcl-95 cells in vitro as determined by annexin-V fluorescence and proportion of apoptotic cells was founddependent on Triphala concentration. MCF-7 cells treated with Triphala when subjected to single cellgel electrophoresis, revealed a pattern of DNA damage, characteristic of apoptosis. Studies on Triphalatreated MCF-7 and barcl-95 cells showed significant increase in intracellular reactive oxygen species(ROS) in a concentration dependent manner. ROS increase was, however, found to be insignificant inMCF-10 F as well as in murine spleen and liver normal cells. In vivo, direct oral feeding of Triphala tomice (40 mg/kg body weight) transplanted with barcl-95 produced significant reduction in tumor

growth as evaluated by tumor volume measurement. It was also found that apoptosis was significantlyhigher in the excised tumor tissue of Triphala fed mice as compared to the control, suggestingthe involvement of apoptosis in tumor growth reduction. These results suggest that Triphala possessedability to induce cytotoxicity in tumor cells but spared the normal cells. The differential effect ofTriphala on normal and tumor cells seems to be related to its ability to evoke differential response inintracellular ROS generation. The differential response of normal and tumor cells to Triphala in vitroand the substantial regression of transplanted tumor in mice fed with Triphala points to its potential useas an anticancer drug for clinical treatment.

Keywords: Triphala; MCF-7; Barcl-95; Cellular cytotoxicity; Apoptosis; ROS; Oxidative stress


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International Journal of Phytomedicine 3(2011) 27-31

Aloe-emodin novel anticancer Herbal DrugKhemkaran Ahirwar1* Sanmati K. Jain1

1 Columbia Institute of Pharmacy, Raipur, C.G.

AbstractThe electrochemical behaviour of the anticancer herbal drug emodin hydroxyanthraquinone present inAloe vera leaves has a specific in vitro and in vivo antineuroectodermal tumor activity. Thecompound does not inhibit the proliferation of normal fibroblasts n or that of hemopoietic progenitorcells. The cytotoxicity mechanism consists of the induction of apoptosis, whereas the selectivityagainst neuroectodermal tumor cells is founded on a specific energy-dependent pathway of drugincorporation. Natural compounds that have traditionally been used to treat a variety of diseases forhundreds of years (1, 2, 3). We assayed only those natural compounds that have already been proven to

be nontoxic, and we evaluated their efficacy against highly malignant tumors that are not normallyincluded in the classical screening assays.

Keywords: Anticancer herbal drug; Emodin; Electrochemical; Dynamics

Indian J Pharm Sci, 2010 Sep-Oct; 72(5): 659-663

I n Vitro Anticancer Activity of the Root, Stem and Leaves of Withania Somnif eraagainst Various Human Cancer Cell Lines

B. Yadav, A. Bajaj, M. Saxena, and A. K. Saxena

Advanced Materials and Processes Research Institute (CSIR), Bhopal462 026, India1 Botany Department, Motilal Vigyan Mahavidhalaya, Bhopal462 016, India2 Indian Institute of Integrative Medicine (CSIR), Jammu Tawi180 001, India

Address for correspondence: Email:[email protected]

Received November 20, 2009 Revised June 21, 2010 Accepted September 30, 2010.

AbstractWithania Somnifera Dunal know asAshwagandhabelong Solanaceae family. It is extensively used inmost of the Indian herbal pharmaceuticals and nutraceuticals. The current study, evaluate in vitro

cytotoxicity in 50% ethanol extract of root, stem and leaves of Withania Somnifera against five humancancer cell lines of four different tissues i.e. PC3, DU145 (prostrate), HCT15 (colon), A549 (lung) andIMR32 (neuroblastoma). Root, stem and leaves extracts showed cytotoxicity activity ranging 098%depending on the cell lines but maximum activity was found in 50% ethanol extract of leaves ofWithania Somnifera. Ethanol extract of leaves obtained from treatments T2, T3, T4 and T5 showedstrong activity against PC3 and HCT15 with 8098% growth inhibition, while the 50% ethanol extractof leaves from T1 treatment showed a minimum of 39% and T3 treatment showed a maximum of 98%growth inhibition against HCT15. This investigation is the first report of the anticancer activity invarious parts of Withania Somniferacultivated in fly ash amended soil.

Keywords: Anticancer, cytotoxicity, fly ash, PC3, HCT15, prostrate, Withania Somnifera


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Asian Journal of Traditional Medicines, 2011, 6(3)

In vitro-evaluation of anticancer and antimicrobial activity of selected medicinalplants from Ayurveda

Rajesh N. Gacche *, Rafik U. Shaikh, Mahesh M. Pund

School of Life Sciences, Swami Ramanand Teerth Marathwada University, Nanded 431 606. (MS), India

AbstractThe use of natural substances to inhibit carcinogenesis is a rapidly evolving aspect of cancer research.In present investigation the ethanolic extract of Argemone mexicana L., (Papaveraceae), Polyalthialongifolia (Sonner.) Thw. (Annonaceae), Terminaliabellarica (Gaerth.) Roxb. (Combretaceae) andTerminalia chebula Retz. Abs. (Combretaceae) were evaluated for their in vitro anticancer andantimicrobial activity. The results obtained indicates that P. longifolia possess a potential inhibiting

activity towards HeLa-B75 [(68.22 0.71) %] HEP-3B [(39.15 0.12)%] and PN-15 [(55.21 0.42)%] cancer cell lines. The selected plant samples were also assessed for their antimicrobial activityagainstEscherichia coli (DH5-), Staphylococcus aureus (MTCC 96),Proteus vulgaris (MTCC 1751),and Candida albicans (MTCC 3017) and the minimum inhibitory concentrations (MICs) weredetermined using microdilution assay. In general, it was observed that the extract of A. mexicana wasfound to be more effective against selected microbial strains. The results of the present findings may beuseful for the discovery of novel anticancer and antimicrobial agents from the plant origin.

Key words: anticancer activity; antibacterial activity; medicinal plants; Ayurveda


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Research Journal of Medicinal Plant 5(6): 728-737,2011

Preliminary Anticancer Screening and Standardization of some IndigenousMedicinal Plants using Cellbiology and Molecular Biotechnology Based Models

1S. Kavitha Bagya,2P.V. Rajashree and 3Kishore Gnana Sam1Department of Biochemistry, Dr. G.R. Damodaran College of Science, Coimbatore, Tamilnadu, India2Department of Biochemistry, Kongunadu Arts and Science College, Coimbatore, Tamilnadu, India3Department of Pharmacy Practice, College of Pharmacy, Gulf Medical College, Ajman, UAE

Corresponding Author: S Kavitha Bagya, Department of Biochemistry, Dr. C.R. Damodaran College ofScience, Coirnbatore, Tamilnadu, India

ABSTRACT

Alcoholic extracts of four Indian medicinal plants Kaempferia galanga (Zingiberacae) Linn.Clerodendrum viocosun (Verbenaceae) Linn., Jatropha curcus (Euphorbiaceae) Linn. And Lensculinaris(Fabaceae) Linn, were subjected to preliminary screening for their antitumor activity. Acutetoxicity studies in mice revealed that all the ethanolic extracts were safe up to a dose level of 500, 1000,2000 mg kg -1body weight. Preliminary short term anticancer screening, by brine shrimp lethality test,

potato disc inhibition and DLA cell line assay, proved that K. galanga, exhibited significant antitumoractivity and it was therefore, selected as a candidate plant for more detailed phytochemical andmechanistic studies. Brine shrimp lethality assay revealed that K. galanga extract inhibited tumordevelopment at a lower concentration LC50= 684.2 g mL

-1as compared to 901, 866 and 5436 g mL-1for the extracts of C. viscosum,J. curcusandL. culnaris, respectively. Alcoholic extract of K. galangasignificantly (p


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Toxicologic Pathology, 36:576-580, 2008

Ursolic Acid and Oleanolic Acid Suppress Preneoplastic LesionsInduced by 1,2 Dimethylhydrazine in Rat Colon

RICARDO A. FURTADO,1RLON P. RODRIGUES,1FELIPE R. R. ARAJO,1 WENDEL L. OLIVEIRA,1MICHELLE A. FURTADO,1MRCIO B. CASTRO,2WILSON R. CUNHA,1AND DENISE C. TAVARES1

1Universidade de Franca, Avenida Dr. Armando Salles de Oliveira, 201 Parque Universitrio, Franca-SP, Brazil2Universidade de Braslia, Campus Universitrio Darcy Ribeiro, Braslia, Distrito Federal, Brazil

ABSTRACT

Ursolic acid (UA) and oleanolic acid (OA) are pentacyclic triterpenoid compounds found in plants usedin the human diet and in medicinal herbs, in the form of aglycones or as the free acid. Thesecompounds are known for their hepatoprotective, anti-inflammatory, antimicrobial, hypoglycemic,antimutagenic, antioxidant, and antifertility activities. In the present study, we evaluated the effects ofUA and OA on the formation of 1, 2-dimethylhydrazine (DMH)induced aberrant crypt foci (ACF) inthe colon of the male Wistar rat. The animals received subcutaneous (sc) injections of DMH (40 mg/kg

body weight) twice a week for two weeks to induce ACF. UA, OA and a mixture of UA and OA wereadministered to the rats five times a week for four weeks by gavage at doses of 25 mg/kg bodyweight/day each, during and after DMH treatment. All animals were sacrificed in week 5 for theevaluation of ACF. The results showed a significant reduction in the frequency of ACF in the grouptreated with the triterpenoid compounds plus DMH when compared to those treated with DMH alone,suggesting that UA and OA suppress the formation of ACF and have a protective effect against coloncarcinogenesis.

Keywords: ursolic acid; oleanolic acid; aberrant crypt foci


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Cancer Research 40, 2661-2665, August 1980

A Double-Blind Study on the Effects of Differing Purified Cellulose and PectinFiber Diets on 1,2-Dimethylhydrazine-induced Rat Colonic Neoplasia

Hugh J. Freeman,

2

Gene A. Spiller, and Young S. KimGastrointestinal Research Laboratory, Veterans Administration Hospital, University of California, SanFrancisco, california 94 121 (H. J. F., V. S. K.]; Cancer Research Centre, University of British Columbia,Vancouver, British Columbia, Canada(H. J. F.); and Syntex Research, Palo Alto, California 94303 (G. A. S.)

ABSTRACTThe incidence, distribution, size, and histopathology of co Ionic tumors induced by parenteraladministration of 1,2-di methylhydrazine were examined in rats fed a chemically de fined fiber-freediet or nutritionally and calorically equivalent diets containing either 4.5 or 9.0% purified cellulose or

pectin. This double-blind study indicates that cellulose is protective against experimental colonicneoplasia. Although the precise mechanism for this protective effect remains to be elucidated, it wasnot cellulose dose dependent and appeared to depend on administration during injection of carcinogen.Furthermore, this study provides strong evidence that identical amounts of cellulose and pectin fed asthe sole source of fiber in chemically defined diets exert strikingly different effects in relation todevelopment of intestinal neoplasia in this animal model.


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Cancer Research 53, 3493-3498, August 1993

Chemoprevention of Colon Carcinogenesis by Organosulfur Compounds

Bandaru S. Reddy,2Chinthalapally V. Rao, Abraham Rivenson, and Gary Kelloff

Division of Nutritional Carcinogenesis B.S. R., C. V. R.and Division of Pathology and Toxicology A.R.,AmericanHealth Foundation. Valhalla. New York I05V5. And Chemoprevention Branch. Division of Cancer Control and Prevention.National Cancer Institute. Bethesda. Maryland 20892 [G. K.I

ABSTRACTIt has been reported that several naturally occurring and related synthetic organosulfur compoundsexert chemopreventive effects in several target organs in rodent models. The chemopreventive actionsof 40 and 80% maximum tolerated doses (MTD) of organosulfur compounds, namely anetholetrithione, diallyl disulfide, W-acelylcysteine, and taurine, administered in AIN-76A diet, onazoxymethane (AOM)-induced neopla sia were investigated in male F344 rats. Also, the effects of

these agents on the activities of phase II enzymes, namely glutathione S-transferase (GST), NAD(P) H-dependent quinone reductase, and UDP-glucuronosyl transferase, in the liver and colonic mucosa andtumors were assessed. The MTD levels of anethole trithione, diallyl disulfide. N-acetylcysteine, andtaurine were determined in male F344 rats and found to be 250,250,1500, and 1500 ppm, respectively.At 5 weeks of age, animals were fed the control diet (AIN-76A) or experimental diets containing 40 or80% MTD levels of each test agent. All animals in each group, except those allotted for vehicle (saline)treatment, were administered AOM s.c. at a dose rate of 15 mg/kg body weight once weekly for 2weeks. All animals were necropsied during week 52 after the second AOM injection. Colonic mucosaland tumor and liver enzyme activities were measured in animals fed 80% MTD levels of each testagent. Colon tumors were subjected to histopathological evaluation and classified as invasive ornoninvasive adenocarcinomas. Colontumor incidence (percentage of animals with tumors) and tumormultiplicity (tumors/animal) were compared among various dietary groups. The results indicated thatadministration of 200 ppm (80% MTD) anethole trithione significantly inhibited the incidence andmultiplicity of both invasive and non-invasive adenocarcinomas, whereas feeding of 100 ppm (40%MTD) anethole trithione or 100 (40% MTD) or 200 ppm (80% MTD) diallyl disulfide suppressed onlyinvasive adenocarcinomas of the colon. Although diets containing N-acelylcysteine and taurineinhibited colon tumor multiplicity, the effect was somewhat marginal. GST, N VI)-(P) H-dependentquinone reductase, and UDP-glucuronosyl transferase activities in colonic mucosa and tumor and liverwere significantly elevated in animals fed anethole trithione or diallyl disulfide, compared to those fedthe control diet. N-Acetylcysteine and taurine slightly but significantly increased only the GST activityin the liver. Although other mechanisms are not excluded, inhibition of AOM-induced coloncarcinogenesis by anethole trithione and diallyl disulfide may be associated, in part, withincreased activities of phase II enzymes such as GST, NAD(P)H-dependent quinone reductase,and UDP-glucuronosyl transferase in the liver and colon.


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European Journal of Pharmacology 637 (2010) 22-29

Chemopreventive potential of ferulic acid in 7,12-dimethylbenz[a] anthracene-induced mammary carcinogenesis in Spragu

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Ayush in Cancer Care (Compilation Work)