Upload
buihanh
View
218
Download
0
Embed Size (px)
Citation preview
Click to edit Master title style
©2017 NuGEN Technologies Inc.
)( )
B 5 5
007/
7-8 0LAA
-C27
qy
qy
l qy 88 9r g t oh e
k moh c
( )
h e g u bmo a pn 5 9A u bmo
k on
n 85 9A u bmok on g v
9NL A85 9A3D L LR
IN F 85
6P NDI T FNL FI3D L LR 9RMNAG
6P NDI T 8 D3D L LR 9RMNAG
6P NDI T FNL FI4ANCRF 9A
3D L LR 9RMNAG
6P NDI T 88 94ANCRF 9A 9RMNAG
FFABLI LBANA1A INR D B
g v
g v
B
B
B
B
w i
5U
LDI 85 9A
6P NDI T DPALM F85 9A 9RMNAG
DPALM F 7FOMG85 9A
B
B
B
B 9D BFA AFF
6P NDI T 9I3I85 9A 9RMNAG
6P NDI T -IG FANA7LIE LRIND 859A 3D L LR 9RMNAG
6P NDI T 0OMDI7 AF LBAN
/ LD CGA N 9RMNAG
B
B
B 6P NDI T 85 9AMRMNAG
V
6P NDI T LBAN/ LD CGA N 9RMNAGB
B•
i qk s d957 2 AF -5
S
S
G 4 G i ei w
ARIML RPA MU 7IBPAP RE (p seyc mb f NGoD 290 w290
o a eARIML 90 EO ( - ( ¥ml dh
290 w n o c te
A1 D 1 2 1 C C 0
p r rg
n G 4 G i ei wn CLPSU g Fi ein c dVa V - LPR L Fvtrn m b h h bN nyO w
® ARIML RPA MU 7IBPAP RE (
UIR MSR NSPIFI ARIML BEAD)**91 *) -+/ +)** */
ARIML RPA MU 7IBPAP RE ( )( )** )( ((+/ +)** *ARIML RPA MU 7IBPAP RE (
UIR MSR NSPIFI ARIML BEAD )( )**91 )( +, (+/ +)** +ARIML RPA MU 7IBPAP RE (/, ASRM ARIML /, )**91 0 )-+/ +)** +ARIML RPA MU 7IBPAP RE (
AIPED 2SA LIOSE 6LDEV 86 /, / */ 0 *,+/ +)*+ +
dh onp o
u o ml reARIML RPA MU 7IBPAP RE ( p 9S539o2I EP4PEE RE LM MG nvx c woc mb rh o n qit
o a n m oa e
b l 4
s op opWorkflow
9S539 0 93 (
cG fg k ekGW n G lswP
n A tm imnGPWd cGO vNi f sn Wapsgt A 2 PrG GO wn C D m imnGOw V M F
S s oO un m imnGP p Gns OTUF g y
E A A
9 PHLKU NFDP / MP ND LC D %(( %%%,- ()) )%
9 PHLKU NFDP / MP ND LC Du bs ihV9 PHLKU PN LS 6HAN NT TOPD %()) (
m h tf w ul cs i hb
9 PHLKU NFDP / MP ND LC DnVv mND D D P NFDP 1KNH G DKP TOPD l tgpb
cGg s o m imnG0 5 C
F08 08 22 1
E CD
83w
w83 PELN
E
A A
vND D D P
, m w u aV %% KFuwiagVv m ND D D P 4 K
5HKL D ¥ o9 PHLKU NFDP / MP NDLC Du g u p
af dtetm w m nVm,- r-, uy ag paf
(8 318 w 981
9SA L V AM D 2 7RI MIET UP E
. )( ) / )( ) -+./ +) )+9SA L V AM D 2 7RI MIET UP E/ , )( )( )( ) -+./ +) ),9SA L V AM D 2 7RI MIET UP E
/, /, )(. /,./ +) )/
9SA L V AM D 2 7RI MIET 25 75 /, / + /, (+
, 5 , fgO Sb bc mrLon
n fgO C , b bc Lmronn eads WNn O ts PRq V i pRvk
9SA L V AM D 6 OAOU UP E o ( in id 1 t l¥n
i 41 m dr u t m rbiapd 9SA L V 80 EN ( - ( . li cgC280 q n t
, + i t drb iapd
-E D G 0GCD
eads C , 5 ,
l fg fg
cg n y v x5 MR t n 280 q
t c t ¥pcg esfs ®nt c u lcm ¥ qspcg
2 F
-E D , 0 A0GCD 1
84 IA LO
-E D , 0 A0GCD
8R438 0 983
G280
L AI 80
-E DG 0GCD C
8O MD GS EM E 6 MCRE 196NEMD E RLM F ,
) ),- ) (( (8O MD GS EM E 6 MCRE 196NEMD E RLM F - ),- ) (( (
n WPN A S - NU FD5A 9 9F 19Cq f kLSv O wn r cid le b L m Rsn 9D D99 F9 AB B RT ag La hLS M f kL
5F BA 3 FD5 B 9F 19C D5D 1 F9
Lb mgl - A
utno no
- mglNU 9F 19Cq f kLSv O w
l/DLNEADM 0 GO LD G t l nuncxU
BD B
8O MD GS EM E 6 MCRE 5D R RLM Flvb GB 17 r ¥
faU l yw at g¥f naU
l ®p ihw naU
8O MD G EM E 6 MCRE 5D R RLM Ffl7N427 1DF 3 M CG E BR qs ur
w gi n meo¥ ® i
¥ faUG F9 BA 9D BA
G F9y 0
7N427 872 )
17 3 3
17 3
® 8M CGFP / 6 BOD O E ) ),- ) (( )
00)1x yN 1 Byn knoM P
n p slpRrM qMn oMg hmi ap f M scSn w R eldM SWX M sc uS/ t
8M CGFP / 6 BOD O Eh FAgu 17 l r Sg R
h f e n���5 0044 i R
fh/C D C 0GFM I CGFf n h ioi qR
cdMfaslp -(
uv uv
00)1 0 6 5 6 0 A D ) D 1 B 5 oMgS O
. 00)1 1 B 1 D
m gCF GIE CM I g f n8M CGF / 6 BOD O E r Sg i R
3GIN I M I d��� 3I AE F g f y e ¥o fi Ri 7 tf m SvS f g y g
ar wS 90 f n g i - 6 I 0 6 R
7 427 sr 872
S W N N N db
n 5 - ef in CMTWCEB 5 B 5 dbn ch G a g
M B5BOETG S09 BMN mW649 prBOETG TO N B 1 l t fol
S A O B N GC OB BMN l x whd asnhl N GC OB hmW dra
ilh gf ETAM ST BOETG TO N B E 1 tdrai i qncf
M B5BOETG S09 A GBmW 1i E 1tdrfol N GC OB BMN hd 6 436 l5BOETG 9B x bsf
hmW bs ®t bend E GB DB B N GC OB NB B D 409 WMBA BA MB MBNB O O N GC OB NB B D 8809 l x ¥qnd
M B5BOETG S09 m lt cnd
/ l N GC OB hmW 1iE 1t drai h neu
0 M B5BOETG S A O B N GC OBBMN pqW 1i E 1l
vx hd
6 436 yx /9 763 ,
7 O U GOM G R 5BOETG 9B 285 GO GBS 9TNOB - R OE M B5BOETG S09 ( ( ( ((
7 O U GOM G R 5BOETG 9B 285 GO GBS 9TNOB . R OE M B5BOETG S09 ( ( ( ((7 O U 8809 5BOETG 9B 9TNOBR OE M B5BOETG S09 ( ( ( (
M B5BOETG S09 A GB ( ) ) ( (
m m nsneu
n m eD vy UG LAl Po T e
4 p - p D
85 pI EPU smb S
EE 4PLDL
E 85
05 L GG
>05 G D G
1G I DI ED D GG 8
4 4D PG D>C G
4 R q D p D L ES
4 RA- ONrtO Da si u U L
5N215 9 51
W GP0 IEC E GL> DI 85 9 R i d h vocr h q h
85 hg muma i e R9 I S R c hi at GP0 IE v i ma 9 I S y eR AA v
R h v maS GP0 IE ~ c h R v uma 9 I ( S GDO L E 85 9 e ,* R9 4 85 9 % e - % h
utcnR uc h R uco fhsma 9 I ) SGP0 IE R3G0 f lud c f o eaS
~hge ud maS1 - 5 D1 0 14 - 5 D
0 14 4 5- D- D
7 IAED 96/ G I C
- , - ,-. ()) .,
( , ( ,-. ()) .-
. , /-. ()) ..
7 IAED 96/ G 3382 I C - , - --. ()
n TD?D5 1.)N uv I T .)n q p A0 1 T 20-)7 IAED 96/ G I C aO64 egu o
u yq p g 16/i gntu P F IEI 96/i) N ls gV¥ P 85/ e 809 eg
i S ntu O R 16/P s Tm imn eg OR
- 5s T. 2 megh lSVt c P
Total RNA500 pg - 50 ng
Ovation® RNA-SeqSystem V2
Ovation® Rapid Library System
Ovation® Ultralow Library System V2
Illumina NGS Platform
.)
m imn
5D ? 1.) 2 A 2GCD 4
c g r o ?D5 1.)
yuv uv
20-) 2 0 -C?D 5 ) 5D ? R (
20-) P .) a fl
6 426 m v / 762 .
RAPGK K9K DM OPDI
6 IA) ,- ) (( * * )
-* ) -* ,- ) (( *RAPGK K9K DM OPDI
:K OD) ,- ) (( *( * )
-* ) -* ,- ) (( *)RAPGK K9K DM OPDI
2NKOKLFGHA) ,- ) (( ** * )
-* ) -* ,- ) (( *
RAPGK K9K DM 1KND 8GP ( ,- ) (( * - )-* ( -* ,- ) (( * )
n 8 8 P D D5 R CD 5 787 taotun E x y 8 8D8 u nn 8 8D8r s v ci qa Tn - 5D 8u n ug d iS eiml LG EHD BDHH ¥v LEu PKPAH 7HH IG A
u t g 5 u xpph t u
m gxh44 3 t 53 u p
2DLHDPD , 7 CDS DAC y i dqtN sr s u t u s g x 1
u dqp ®s dqap xh 2DLHDPDv u pt h o s u a ph
0 5D . 8A CD8 C8 8 P – R 8Ataotu S
1KND 8GPtv 2DLHDPD v x xi2DLHDPD uyu xh e c ¥
i mf pv–I P
4 D5
N O
, 7 CDS DAC vt 1 u
tgxh u 1¥ tv 2u
t s v pxg a 0 1u
t nu ap m tv ap
xi pg 53 u- 78G 857pv
t c, uu t
n a 1t u gxh ddpv q 0v u
lxha ,7 CDS DACa t
q xh g gq 1t ¥ov u
n , 7 CDS DACp 1 p
q xh , P 01, ,
,
- 78G 857 1
53 3
% ±8O D G GDO E 971 : :
, )( ) ) )()-./ ) -
8O D G GDO E 971 : :/, /, / ( 1
./ ) (
k kg Wni 0( F s R t
n k kg i D A 0(Wn G DC Ag s Rqn iG 0( hl –i q(CM EA hmon 2 mo 0(u hl
S t –i(CM EA w R q re okar h(CM EA s Rq dTok 0 - 0 e RvR U h p s Rq e
k s R 0 - 0 e k P e dU c fl eiG 0(i ®q f (CM EA s R t l Uk Rw
q c U
1 DCO C G A 0( F M
yR u D A 0(N C
7DG ADL
8O D G GDO E 971 : : lSsaG E 971 p u ¥ f
l Ui971 S 971ih it q7N547 1G 3 IE
0 6G31 2 t q g¥ f 971Svy f1G 3 IE u t q g
fS ®t sa t qg¥ f
1G 3 IE
1G 3 IE
1G 3 IE CG E1G 3 IE t si S 971 om E DG
rq lSbrcr ( /R ( ,om . .R. fSDGA DO ¥sa
() )R- / f r S 1G 3 IE te S 971 om E DG lSbrcr
/ R ( om (R nf S DGA DO¥./ .R ( n
7N547 u 1: 874
G G C MC
DC G CMC L
0 A D (C AD
G C DC
C E G
. DC L 0 A D(C AD G ( E DG
G C A DC
(CM EA( E DG )A
2)
1A D 5 CC D
2G C
5 GCL C
5G C G EG 0(
¥ 8M BFEP 6FL 7 H O D (, ),- ) (( )8M BFEP 7 H O D (- ),- ) (( )8M BFEP 0 F FGAB 7 H O D ) ),- ) (( )(8M BFEP B FG B 7 H O D ) ),- ) (( ))
¥ 8M BFEP F5F 7 HEO0 G 9 BD 6F L 4LD E
),- ) (( )
8M BFEP F5F 7 HEO0 G 9 BD 6F L 6FL
)(,- ) (( ),
8M BFEP F5F 7 HEO0 G 9 BD 6F L 0 F FGAB
)),- ) (( )-
n i l a d o vOB B 0)AB 6 6Ml glmH
) E+ B l iH R 0) tl glmH a
1D B F 0) ) E+ B 6
¥ 8M BFEP 4LD E / FF 7 H6L BG N O D , ),- ) (( (8M BFEP 4LD E / FF 7 H6L BG N O D -
, ),- ) (( ((
¥ 8M BFEP 4LD E 2291 7 H6L BG N O D ,
( ),- ) (( ()8M BFEP 4LD E 2291 7 H6L BG N O D -
( ),- ) (( (
nq
n --2, P 8 y P 8OB B 0)
6n ea 0) ( ( S o p s ) )
+ ) E+ B rN u
--2,
) E+ B 6
7L317 R S 871
® 8P NDI S 0IG N 9LIE LRIND:7/ 1: 6O ND RMN G , ),- ) ((8P NDI S 0IG N 9LIE LRIND:7/ 1: 6O ND RMN G - ),- ) (( ,
eqb RP5 D O 0 A kn
n eqb liPD D RCD 5 5O Sknn eqb liP P S ( D moNrgn a p D D pN L
W a p D D
hfcd cd
-F D D D 0 A 0 CD
1 7
8P NDI 0IG N 9LIE LRIND :7/ 5D L LR RMN GMm
0I NLI L AIG LDG L so8P NDI 0IG N9LIE LRIND :7/ 5D L LR RMN GMx g c d CmNIN :7/¥t y m ®x paeU m30
x l ag lL:7/x ag v ix ag pbU
x ibv8P NDI S 0IG N 9LIE LRIND :7/5D L LR RMN Gn k bv ¥t ®ae
NIN :7/¥t y m ® bUL:7/ m kx bver 7O327 m/ R1 N
4 1/ 0 x ag pbU ® n k lbver / R1 N y nL:7/m lag g pbU e v ln8P NDI S DP LM :7/ RMN G ( k x g/ R1 N y x bv i su
x q i pbU
( D
7O327 ® y / 872
CD 0D 5 0 D C CD 0 D F
0 5 0D 5 0 D C CD E D 5
D D
) 5
D D E D 55 5 D C
0D 5 0 D
5 D F( D
E M I 8 D96/ H
- - - )-- ()) ,)
( - ( +- ,- ()) ,
+ - + ) +- ()) ,+
E M I 8 D96/ HN 3 D E A E /EO0 G
- (( -- ()
( (( ( (,- () (
+ (( +- () )
E M I 8 D96/ H D( (
+ +
E M I 8 D96/ H 04 54 + )) + +
E M I 8 D96/ H 04 54 N 3 D E A E /EO0 G + ), + -,
P P gp R 1 kc cdFq
n U . 7 R . 010 1 kc cdF q vrn 5 1 11 1 7 N W a F F t c cdFn 1 c cdF Rs u np 1 71 1wyN S mvr
5A1 .7 7 1
F fae .7D
olhi hi
- 7
E M I 8 D96/ HcP an ru wRmpvR D96/ H 6 216yb/EO0 G a PD96/ Hs qstRUg a
DimerFree
mRNASelection
AnyDeplete
Transcript Depletion
cDNA Synthesis
no adaptor dimer unwanted transcript
removal
rRNA tRNA
+mRNA AAAAA
AAAAA
AAAAAAAAAAAAAAA
AAAAA
AAAAA
rRNA tRNA
AdaptorLigation
oligoT beads
ae S i S
6 216y l o / 761 )
MF 7FOE 3PH I M 7 IR KG O
, ) , ,- ) (( , )) ,- ) (( - ) )
- ) - ,- ) (( + () )
MF 7FOE 6 PN M 7 IR KG O
, ) + , ,- ) (( + )) + ,- ) (( + ) )
- ) + - ,- ) (( + () )MF 7FOE APNO H IR KG O KM N O c
MF 7 4FOE 3PH I M 7 IR KG O - - ( - )
F- kT PT l N PT a S
n 0.) 70 7 .D E 7 5 ) A 5E S W )n 7 77E7 S W e f T R g ghn ) 7A7E7S mw t 1 )R TqrRh c ypS s
3 1 ) 7
ei E E5 1 )A I
urno ( noG
Trio RNA-Seq
®
www.nugen.com
M01441v1
High sensitivity whole transcriptome analyses
Trio RNA-Seq offers a complete, best in class, whole transcriptomics solution for FFPE, challenging samples and detection of rare transcripts. Designed for robust and reliable transcript analysis, Trio RNA-Seq can help you consistently capture more information from virtually any sample. Trio RNA-Seq integrates the proven technology from the highly published Ovation® RNA-Seq System V2 to provide a complete workflow from total RNA to library.
Why use Trio RNA-Seq?
Trio combines three powerful technologies:
1. Single Primer Isothermal Amplification (SPIA) enables better access to limited and degraded samples.
2. Enzymatic fragmentation and DimerFree library construction allows for more efficient and robust library preparation.
3. Customizable transcript depletion with AnyDeplete maximizes informative sequencing reads from whole transcriptome data.
This powerful combination helps increase sensitivity while maintaining unbiased libraries
with no adaptor dimers, improving detection of rare transcripts and decreasing sequencing costs via higher multiplexing.
Features
Integrated DNase treatment
Unbiased, highly reproducible RNA amplification
Enzymatic fragmentation
Efficient library preparation with unique barcodes for every sample
Customizable transcript depletion after library preparation
Automation friendly
Figure 1 Trio RNA-Seq is a complete workflow, incorporating three proprietary technologies.
lq oaT c e T
MF 7 nS 1190 s dO O G 7 w l gSw dpm haT l hS Um l d
7 haTodS w dA 7 m i FH M1M m y lrtS l y m w i haT
5 ) mO O G 7 m) KDS) IDS) IDwy i gS MF 7
w g y w odT ln l cg
y d w g oaTylrsbS
sug oaT
7P207 y 870 )
n EGo EF d Diy lnn 0 p EF g wr kAn EF maA T
->>9; A 5 ;9E 9 AE ; 0 0u ow e
32 3 ;>9 2 9 < 9 E9 < A>A; g 302g
u ow Se302s uh m O S
0
P SN t
fe
ma PS W P n
->>9; A 5 ;9E9 9 AE ;
2 A69302
)( )( N T
) ) N T->>9; A 5 ;9E9 9 AE ;
2 A69302
)( )( N T
) ) N T->>9; A 5 ;9E9 9 AE ;
2 A69302
)( )( N T
) ) N T->>9; A 5 ;9E9 9 AE ;, 2 A69 N T
1
->>9; A 5 ;9E 9 AE ;g Se b
302gbd Se OS u ow Se302 f f
A G5 95 6 fea r n t hl g c
u pt i O P S
0 0 hy -3 10 (
n iu 0)V 0 . 0 42 5 nPipran Wb O r s ciu Ofmn nPipr
/ n Pgv P R( e l r P S
3 G D K 7 E G E G 6 FG i 2 2 h N cR 64 7 6 A4E E E G 7 DAD s Sb h 2 Vo N s by u Ns lh c M N N s c eaoh
N cm h N g u y h u Ns c MN h W S N s M g N h N i
Tor M64 7ieh h N g bm 0 O 1 h N u gc M
Eco nP iPl
Pw2 yP ctP hnPipr
4C A 2F F CF C 5 CDAD 42 5 a nPipr lm
1 8 DCN 58F CF C 4 G
mnPrk 0) M C
– ( T
nPipr Pwco nP ctP h
42 5 DF 0)® 2 W g M. t Ns r rh 2 ® g u N M5 8t NW N N Pv u s T N u u N
MN h s S M
. n 5 8t Ng r rh u Ns Sb M( N W r u NW r M
DF AD
2 2 u 6 32 )
n 0)T 0 . 0 42 5 kPfn an akPfnn i e mwPsW S Pdr P ( 8FGt gP –6M D GO 1 D G G E IB 0GID CF G 9N F i 5 205 h S cT9 0 9DGBE IDF I 0GID CF G CG E BN t ®h E 85 o S t
St amh c RyS Sh. E B A 9 F D 4 D G DG . G I .6943. d.CDF I - h S
S c µ d r ( h µ h t S g uc l Rs GBh E 85 h6M D GO 5 4 E A I IB 0GID CF G fe lai11 0 oh cTr PBh E 85 c Sh c R
F 0) O ltkPycfPi
0)
1 8GDCM FDC28C A58E G CE CG4 FG
kP h t GDG8A 0) C N
( R
t Ps2 tycuP P ekPfn
kPfn t PsltkP P e
4C A 2E E CE CG5 CDAD 42 5 a RVkPfn i ov
42 5 DE 0)5 gf l R
1 v St r rh 5 g S l R80 v S a S S t V S d S l R
S h t l R( 1 Wn 80 v Sg r rh St l R) S ra S rl R
DE AD
1 8G DCM 0) /D ADE 58E G CE CG
5 205 y 9 650
6 6 - - ACP S NORT
() - 6 6 EAI B
- 6
9 OG T 1PNO I
MECO 3 MGAFIC O SNOCId
- d
9 OG T A28/ 7 BP CD M MECO 3 MGAFIC O
, - , ),- ) ()- ),- ) () (
- - - ),- ) () )
9 OG T 4PNG : CMECO 3 MGAFIC O SNOCI
, - ( , , ),- ) ()- ( ( , ),- ) ()
- - ( -,- ) () ,
9 OG T 4PNG : CMECO 3 MGAFIC O SNOCIG E C CR 3 MGAFIC O
, - ) , ),- ) () -- ) (, ),- ) ()
- - ) - ),- ) ()
8 6 BCR C B o m:1 BP GA OCn m pcV n:1 e mo 0ns m luy o p e / 0 1ns my ge mopdx eV8P538 n8 6 BCR C B o m n n mst hy
:1 BP GA OCmsurn¥ pcV o / 0 o ny fpc 86 BCR C B lue avpcV ¥ / 1m io ny ¥h8 6 BCR C Br
ue :1 BP GA OC u avpcV, O 01 , 8 V
8P538 y / 983 -
2S
VT
R
WQ
SV
T
CFI
DFF
5L
CBO
E
FF5L
CBO
E
FF5L
CBO
>I
>G
E
AFC>
CFF
E
FC
2*>
CC0O
>
G
--
E
OD>
IBN
>E>*
ABG
PII
O3M
BFE
2D
GO
>D
EFI
>BF
BE1F
LC>
3M
BFE
2D
GO
>D
EFI
>BF
BE1F
LC>
3M
BFE
BF
O>D
EFI
>BF
BE1F
LC>
3M
BFE
2O
>D
3M
BFE
>>E
EFI
>BF
BE1F
LC>
3M
BFE
3E>
BI>
O>D
EFI
>BF
BE1F
LC>
3M
BFE
--O
>D
EFI
>BF
BE1F
LC>
OD>
IBN
>E>*
ABG
NFE
>E>
IIO
GGC
L>
D
GCL
O>D
EFI
>BF
BE1F
LC>
3M
BFE
BF
O>D
3M
BFE
BF
0O
>D
EFI
>BF
BE1F
LC>
3M
BFE
BF
O>D
3M
BFE
BF
0O
>D
EFI
>BF
BE1F
LC>
3M
BFE
BF
O>D
3M
BFE
BF
0O
>D
EFI
>BF
BE1F
LC>
3M
BFE
3E>
BI>
O>D
EFI
>BF
BE1F
LC>
3M
BFE
--O
>D
EFI
>BF
BE1F
LC>
BC>E
LC 1F
>>E
>NG
I>BF
EII
O
3M
BFE
2D
GO
>D
2L
2P
BC>E
FCL
BFE
GGCB
BFE
2F
>
3M
BFE
2D
GO
>D
2L
2P
BC>E
FCL
BFE
GGCB
BFE
2F
>
3M
BFE
BF
0O
>D
2L
2P
BC>E
FCL
BFE
GGCB
BFE
2F
>
3M
BFE
2D
GO
>D
2L
2P
BC>E
FCL
BFE
GGCB
BFE
2F
>
3M
BFE
3E>
BI>
O>D
2L
2P
BC>E
FCL
BFE
GGCB
BFE
2F
>
3M
BFE
--O
>D
2L
2P
BC>E
FCL
BFE
GGCB
BFE
2F
>
/CCL
DBE
AFC>
>EFD
>NG
I>BF
E>
*ABG
3M
BFE
2D
GO
>D
EFI
>BF
BE/0
1F
LC>
3M
BFE
2D
GO
>D
EFI
>BF
BE/0
1F
LC>
3M
BFE
BF
0O
>D
EFI
>BF
BE/0
1F
LC>
3M
BFE
2D
GO
>D
EFI
>BF
BE/0
1F
LC>
3M
BFE
3E>
BI>
O>D
EFI
>BF
BE/0
1F
LC>
3M
BFE
--O
>D
EFI
>BF
BE/0
1F
LC>
5L
EB
BM>
*3M
BFE
*O
>D3M
BFE
BF
O>D
3M
BFE
BF
0O
>D
3M
BFE
*O
>D3M
BFE
3E>
BI>
O>D
3M
BFE
--O
>D
������ �� ��������� �����
A0
2
* ) (
ge af i e
4 AE L 5A E 8- 7 G 9, ( ) (
) ) , ( (,) - , ( ( (
O 5 0 5 DSD P
0 B A
ge af i e
4 AE L 005 8- 7 G, (
) ) , ( , , (,) - , ( , (
RVT 0 P
0 B A
ge af i e
4 AE L 63- - FBA A AE 7 G 9, ( (
) ) , ( )) (,) - , (
ENFLW 0 RVT
0 B 0 A
ge af i e
4 AE L 6 ) ) , ( ,,) - , ( (
W 0 RVT
0 B 2
geT 4 AE L 63- - FBA A AE 7 G 9 S¥V Rc O PN
ge af i e
4 AE L 5A E72 8- 7 G 9 , ( ) (, ( (
O 5 0 5 DSD P
0 B A
3 1 3 ge W -7 43
url ot v rv mq p 7M B EO 7EA 0BIA N AD ( , ( , (
R Oql- IQN imM
A D B
url ot v rv mq p 7M B EO 8 9 N AD , ( , (
- pN PNE - IQN imW
A D - B
url ot v rv mq p -GGC L AO -DG 8CL N AD (( , ( ,) (
a dL - IQ Sim R eM n
D / / B
url ot v rv mq p
1E IAO B BE 5 LCA, ( , (
) ) , ( , (,) - , ( , (
1E IAO B BE34 5 LCA , ( ,( ,
cT pN PN N ogC d MD
6L216iur - 761
S y Rmqa n Ws ed Tc P W¥
ipgjL
xQ GNgp
GN Ca
) ) ) ( ( !MNS
C a ) ) C a len a ro nMNS a MNS
n rm7041.8
P boTeb cal
�� �
G i a M
G i t
C C N
X
h s t7041.8
26 HHE 9I A 9 E H H F:0 B9 A /35O 9 E