Avenanthramides

in Avena Genetic Resources

Lena Dimberg

AvenanthramidesAvenanthramides

R

R

NH

O

OH

R

R

COOH

n

R= H, OH or OCHR= H, OH or OCH33

n= 1 or 2n= 1 or 2

Project Working Collection

Species 2008 2009

A. sativa (54)* 287 256

A. byzantina (2) 8 16

A. strigosa (5) 15 31

A. abyssinica (1) 2 3

A. barbata (1) 3 1

A. canariensis - 1

A. damascena (1) 2 -

A. fatua (3) 7 9

A. hirtula 1 3

A. hybrida (1) 1 -

A. sterilis (1) 2 3

A. wiestii (1) 1 -

329 323

*70 oat samplesused for

quantification of avenanthramides

Year 2008: Bulgaria, Estonia and Polen

Year 2009: Italy, Sweden and Romania

The content of the avenanthramides The content of the avenanthramides 2c2c, , 2p2p and and 2f2f in in 70 oat samples grown in Bulgaria 200870 oat samples grown in Bulgaria 2008

Oat samples

90-3870 mg/kg

The content of the avenanthramides The content of the avenanthramides 2c2c, , 2p2p and and 2f2f in in 70 oat samples grown in Bulgaria 200870 oat samples grown in Bulgaria 2008

Oat samples

A. strigosa 90-3870 mg/kg

The content of the avenanthramides The content of the avenanthramides 2c2c, , 2p2p and and 2f2f in in 70 oat samples grown in Bulgaria 200870 oat samples grown in Bulgaria 2008

Oat samples

”Wild species” 90-3870 mg/kg

Avenanthramide content in different Avenanthramide content in different types of oat genetic resources types of oat genetic resources

(Bulgaria, 2008)(Bulgaria, 2008)

Avenanthramide [nmol/g]

n Min Average Max

Modern 20 337 1463 a 4947

Cultivated obsolete 36 313 1040 a 3013

Marginally cultivated 6 1527 6517 b 13603

Wild (Poland, 2008) 8 545 2071 c 3564

Table 8.1

Preliminary correlations between AVAs and various quality traits (Bulgaria 2008)

Yield TGW Test weight

Husk Prot Fat Starch Total -glucan

nPearson correl

63-0.34**

62-0.43**

63-0.38**

63-0.08ns

410.10 ns

410.11ns

67-0.13 ns

650.10ns

Content of 2c, 2p and 2f in oats grown in Content of 2c, 2p and 2f in oats grown in Bulgaria and Estonia, 2008Bulgaria and Estonia, 2008

Year variation of avenanthramidesYear variation of avenanthramides

0

100

200

300

400

500

600

2c 2p 2f

Co

nce

ntr

atio

n (

pea

k ar

ea/g

)

s

r

Avenanthramides in crown rust Avenanthramides in crown rust susceptible and resistant cultivarssusceptible and resistant cultivars

Correlation between DON and AVA Correlation between DON and AVA in Fusarium inocculated standard cultivarsin Fusarium inocculated standard cultivars

Correlation between DON and AVA Correlation between DON and AVA in non (or natural) -inocculated standard cultivarsin non (or natural) -inocculated standard cultivars

Total amounts of avenanthramides Total amounts of avenanthramides 2c2c, , 2p2p and and 2f 2f in eleven standard oat cultivars (2 naked, 9 in eleven standard oat cultivars (2 naked, 9

covered) covered)

FusariumNon or naturally inocculated (□)Artificially inocculated (■)

Avenanthramide content in oats with Avenanthramide content in oats with or without or without Fusarium Fusarium inocculation inocculation

(mean of 11 oat cultivars)(mean of 11 oat cultivars)***

******

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