Automatic registration,seamless mosaicking

and applications

D. Fedorov, B. Sumengen, B. S. Manjunath2005, Vision Research Lab, UCSB

This research was supported by NSF ITR #0331697

Vision Research Lab, UCSB 2/17

Seamless mosaic of 12 confocal images with variable depth in each image through the sample of human fovea.

Robust registration and seamless mosaicking

14 confocal 3D images of 2XKO10 mouse retina

+ =

Vision Research Lab, UCSB 3/17

Robust registration and seamless mosaicking

enhanced mosaic

Original mosaic of 9 confocal images of 7 day detached cat retina stained with TOPRO

Vision Research Lab, UCSB 4/17

Registration for multi-channel imaging

Z + Ch

Novel technique for acquisition of multispectral images using automatic and high quality registration of images acquired with variable depth position and staining. Automatic registration dramatically increases the speed and quality of obtained results.* Images provided by Robert E. Marc and Bryan W. Jones from John Morgan Eye Center, Utah.

B01

E01

TT01

Vision Research Lab, UCSB 5/17

Local enhancement - confocal images

Histogram equalization

Original image

Local enhancement

Vision Research Lab, UCSB 6/17

Local enhancement - confocal images

Original image with non-uniform TOPRO staining in the ONL layer

Enhanced image ready for cell counter and texture analysis

Vision Research Lab, UCSB 7/17

Dynamic range compression

Original 12 bit image

Microtubule Stacks: Dynamic range compression - space and time enhancement. Spatial luminance decay is corrected.

Local enhancement

Vision Research Lab, UCSB 8/17

Microtubule Stack EnhancementThe photo-bleaching effect responsible for gradual fluorescence decay was corrected for the entire stack (30 frames) globally and locally

Entire image

region of interest

Vision Research Lab, UCSB 9/17

Multi-focus with optical microscope

Three of 9 images of radioleria multi-focus result

depth map

Vision Research Lab, UCSB 10/17

Multi-focus

High Resolution Through-Focus Movie of 40 frames through the dorsal lateral geniculate nucleus

in-focus result

Vision Research Lab, UCSB 11/17

Multi-exposure imaging

gradient domain, Fattal...gradient domain, Fattal...mosaic based, our resultmosaic based, our resulttone mapping, Ward Larson...tone mapping, Ward Larson...