Opal Multiplex Automation IHC Detection KitsIncrease your study throughput and benefit from the consistency of automation by performing Opal™ multiplex staining on one of the leading research automated staining platforms – Leica Biosystem's BOND RX.

With the push of a button, the BOND RX will run entirely unaided to produce up to 30 beautiful seven-color immunofluorescence slides in about 14 hours, providing you with the flexibility to support the dynamic demands of translational research.

Automating Opal staining brings the benefits of:

• Quality, consistency, and reproducibility with every sample

• Time-savings vs. a laborious manual process

• Ease-of-use with a walkaway, high-throughput protocol

AUTOMATE YOUR MULTIPLEX IHC

BOND RX by Leica Biosytems Opal Multiplex Automation

IHC Detection Kits

Breast cancer tissue stained on the BOND RX with Opal Multiplex IHC Detection Kits for Automation. Cytokeratin (blue), CD68 (purple), CD20 (red), CD8 (yellow), CD4 (green), FoxP3 (orange).

D.A.

E.B.

F.C.

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Opal Multiplex IHC Detection Kits for Automation

Detection kits include Opal fluorophores, DAPI, 1X Plus Automation Amplification Diluent, 1X Antibody Diluent, and either Opal Polymer HRP Ms+RB or Opal Polymer anti-Rabbit HRP detection reagent.

PRODUCT FLUOROPHORE SIZES PRODUCT NUMBER

Opal 4-color Automation IHC Kit

DAPI, Opal 520, Opal 570, Opal 690 50 slides NEL820001KT

Opal 7-color Automation IHC Kit

DAPI, Opal 520, Opal 540, Opal 570, Opal 620, Opal 650, Opal 690 50 slides NEL821001KT

Opal 4-color anti-Rabbit Automation IHC Kit

DAPI, Opal 520, Opal 570, Opal 690 50 slides NEL830001KT

How Does Opal Work?

Opal follows the standard IHC workflow using unlabeled primary antibodies, followed by the addition of anti-species-HRP conjugate and detection substrate. Opal fluorescent detection substrates bind covalently near the epitope, allowing subsequent antibody removal or inactivation to clear the tissue for detection of the next target. The signal remains stable after antibody removal.

The Phenoptics™ Workflow – A Complete Solution

Mantra™, Vectra® and Vectra® Polaris™ imaging systems use multispectral unmixing to provide quantitative results for each marker in a multicolor IHC slide, making them ideal for imaging and analysis of Opal samples. When combined with inForm® analysis software, this novel solution enables automated cellular phenotyping and tissue segmentation for virtually unlimited investigation of the biology expressed in your samples. The complete workflow – from immunostaining, through imaging and analysis – is known as Phenoptics.

Vectra® Polaris™ Automated Quantitative Pathology Imaging System

For more information please contact your local sales representative or visit www.perkinelmer.com/Phenoptics

All kit components are also available for sale individually.

Opal, Vectra, and Mantra products are for research use only and not intended for use in diagnostic procedures. BOND RX is for research use only. Not for use in diagnostic procedures.