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Dr. Roshni Rajamohan
ATP Synthesis
Dr. Roshni Rajamohan
Department of Botany
Deshbandhu College
Dr. Roshni Rajamohan
ATP Synthesis• Mechanism of ATP Synthesis
• Substrate level phosphorylation
• Chemiosmotic mechanism (oxidative and photophosphorylation)
• ATP Synthase
• Boyer’s conformational model
• Racker’s experiment
• Jagendorf’s experiment
• Role of uncouplers
Dr. Roshni Rajamohan
Mechanism of ATP Synthesis
1. Oxidative phosphorylation-It is the chemiosmotic synthesis of ATP associated with the transfer of electrons through the electron transport chain (from NADH / FADH 2 to O 2 by a series of electron carriers) and the accompanying consumption of oxygen. ATP is formed as a result of the transfer of electrons This process, which takes place in mitochondria, is the major source of ATP in aerobic organisms.
2. Photophosphorylation- The light-dependent chemiosmotic synthesis of ATPin the chloroplast in the presence of light. ATP is formed as a result of the transfer of electrons which takes place in chloroplast, is the major source of ATP in photosynthetic organisms
3. Substrate level phosphorylation-ATP synthesis by the transfer from a high-energy compound (phosphate group) ADP, without involvement of any electron transport is called substrate-level phosphorylation.
Dr. Roshni Rajamohan
Photophosphorylation Light-dependent ATPsynthesis- coupled reaction
• This process was discovered by Daniel Arnon and co-workers in 1950s
• Electron flow without accompanying phosphorylation is said to be uncoupled.
• Photophosphorylation works via the chemiosmotic mechanism, first proposed in the 1960s by Peter Mitchell.
• The total energy available for ATP synthesis, called the proton motive force (∆p), is the sum of a proton chemical potential and a transmembrane electric potential, ∆p = ∆E − 59(pΗi − pΗο)
• A transmembrane pH difference of 1 pH unit is equivalent to a membrane potential of 59 mV.
Dr. Roshni Rajamohan
Substrate-level phosphorylation
• ATP synthesis by the transfer from a high-energy compound (phosphate group) ADP, without involvement of any electron transport is called substrate-level phosphorylation.
• In simple terms, it is the production of ATP by the transfer of a phosphoryl group from the substrate of a reaction to ADP.
• Substrate-level phosphorylation differs from the other two ways of ATP synthesis i.e., oxidative-phosphorylation and photo-phosphorylation, in that the ATP synthesis is not coupled to any electron transport.
• Oxidative-phosphorylation and photo-phosphorylation involves ATP synthesis coupled with electron transport.
Dr. Roshni Rajamohan
2 Steps in Glycolysis where substrate level phosphorylation occurs
Dr. Roshni Rajamohan
• In the first reaction, energy is harvested in the form of ATP. The enzyme phosphoglycerate kinase catalyzes the transfer of the phosphoryl group of 1,3-bisphosphoglycerate to ADP. This is the first substrate-level phosphorylation of glycolysis, and it produces ATP and 3-phosphoglycerate. It is a coupled reaction in which the high energy bond is hydrolyzed and the energy released is used to drive the synthesis of ATP.
• The final substrate-level phosphorylation in the pathway is catalyzed by pyruvate kinase where Phosphoenolpyruvate is coverted to Pyruvate. Phosphoenolpyruvate serves as a donor of the phosphoryl group that is transferred to ADP to produce ATP. This is another coupled reaction in which hydrolysis of the phosphoester bond in phosphoenolpyruvate provides energy for the formation of the phospho-anhydride bond of ATP.
Dr. Roshni Rajamohan
ATP synthase
• The ATP is synthesized by a large (400 kDa) enzyme complex known by several names: ATP synthase, ATP ase (afterthe reverse reaction of ATP hydrolysis), and CFo–C1 (Boyer,1997).
• This enzyme consists of two parts: a hydrophobic membrane-bound portion called CFo and a portion that sticks out into the stroma called CF1.
• CF1 is the portion of the complex that synthesizes ATP. CF1 is made up of several peptides, including three copies of each of the αand βpeptides arranged alternately much like the sections of an orange. Whereas the catalytic sites are located largely on the βpolypeptide, many of the other peptides are thought to have primarily regulatory functions.
• CFo appears to form a channel across the membrane through which protons can pass.
• The molecular structure of the mitochondrial ATP synthase has been determined by X-ray crystallography (Stock et al. 1999). Although there are significant differences between the chloroplast and mitochondrial enzymes, they have the same overall architecture and probably nearly identical catalytic sites. In fact, there are remarkable similarities in the way electron flow is coupled to proton translocation in chloroplasts, mitochondria, and purple bacteria .
• Another remarkable aspect of the mechanism of the ATP synthase is that the internal stalk and probably much of the CFo portion of the enzyme rotate during catalysis (Yasuda et al. 2001). The enzyme is actually a tiny molecular motor.
Dr. Roshni Rajamohan
Structure of ATP synthase
Dr. Roshni Rajamohan
ATP synthase- A tiny molecular motor
ATPase or CFo–CF1
• CF1 is the portion of the complex that synthesizes ATP.
• CFo appears to form a channel across the membrane through which protons can pass.
• It is a large multi-subunit complex, CF1, attached on the stromal side of the membrane to an integral membrane portion, known as CFo.
• CF1 consists of five different polypeptides, with a stoichiometry of α3, β3, γ, δ, ε. Cfo contains probably four different polypeptides, with a stoichiometry of a, b, b′, c12.
Dr. Roshni Rajamohan
• Model of the FoF1-ATPase, showing the attachment of the catalytic complex to the membrane via the β subunit and the δ subunit.
• When the reaction runs in reverse (ATP synthesis), protons diffuse through the Fo complex down their electrochemical gradient. The movement of protons through the channel drives the rotation of the entire Fo complex within the membrane.
• The γ subunit, which is attached to the Fo complex, then turns within the catalytic complex, causing the conformational changes that are required for ATP synthesis.
• It is assumed that the catalytic complex itself does not rotate, but is anchored to the membrane. The δ subunit is located on the outside of the β subunit and serves as the site of attachment of the β subunit, which anchors the catalytic complex to the membrane and prevents it from spinning.
• In mechanical terms, the F1 complex and its membraneanchor act as a stationary housing, or “stator,” while γsubunit (and possibly the Fo complex) serves as the “rotor.”(From Junge et al. 1997.)
Dr. Roshni Rajamohan
Uncouplers / Protonophores• Uncouplers are amphiphilic compounds(which are soluble both in water and lipids). They are
agents with conjugated double bonds which allow them to diffuse across the membrane in both the protonated form and the unprotonated form, and thus dissipate the electrochemical proton gradient.
• Uncouplers which transfer protons across the membrane are known as protonophores.
• They disrupts phosphorylation by dissociating the reactions of ATP synthesis from the electron transport chain. They directly bypasses the ATP synthase by allowing passive proton influx, without affecting electron flow, but ATP synthesis does not occur.
• The result is that the cell or mitochondrion expends energy to generate a proton motive force, but the proton motive force is dissipated before the ATP synthase can recapture this energy and use it to make ATP.
• Uncouplers increases the proton permeability of the inner mitochondrial membrane and dissipates the proton gradient. Uncouplers are capable of transporting protons through mitochondrial and chloroplast membranes.
• Both mammalian and plant mitochondria contain uncoupling protein (UCP). This protein facilitates the movement of protons across the inner membrane and therefore partially uncouples electron transport and decreases the ATP yield of respiration. Electron flow without accompanying phosphorylation is said to be uncoupled.
• Uncoupling proteins (UCPs) occur in the inner mitochondrial membrane and dissipate the proton gradient across this membrane that is normally used for ATP synthesis.
Dr. Roshni Rajamohan
Role of uncoupling agents
• Addition of uncouplers results in continuation of electron transport and proton pumping, without generation of any proton gradient. ATP synthesis does not occur without affecting uptake of oxygen. In the absence of proton gradient, however, protons are transported in reverse direction through ATP synthase at the expense of ATP. Protonated DNP (a weak acid) diffuses from high proton concentration side of the membrane to low proton concentration side where it gets dissociated to generate protons resulting in dissipation of proton gradient. Membrane is permeable to both protonated and anionic forms of these.
• E.g. FCCP (trifluoromethoxy carbonyl cyanide phenylhydrazone), a very efficient mitochondrial uncoupler. Other exampes of uncouplers- Carbonyl cyanide phenylhydrazone (CCP) 2,4-dinitrophenol (DNP), Carbonyl cyanide m-chlorophenyl hydrazine (CCCP)
Dr. Roshni Rajamohan
The mechanism of action of uncouplers
(B) In the presence of an electrochemical proton gradient across the membrane, FCCP will become protonated and thus pick up a proton on the positive side of the membrane, move across the membrane in the neutral form, and lose the proton on the negative side. This is driven by the electrochemical proton gradient. In the negative form, FCCP will be driven back out across the membrane by the membrane potential. The net result is the movement of one proton from the positive to the negative side of the membrane, which results in the dissipation of the electrochemical proton gradient, and the movement of one electrical charge from the negative to the positive side of the membrane, which results in the dissipation of the electrical gradient. The shaded area represents the system of conjugated double bonds (π-orbital system). (Modified from Nicholls and Ferguson 1992.)
(A) The protonation / deprotonation of FCCP which is a weak acid.
Dr. Roshni Rajamohan
CHEMIOSMOTIC THEORY
• Peter Mitchell in 1961 proposed CHEMIOSMOTIC THEORY
• Nobel Prize for Chemistry in 1978
• Membrane potential with high negative charges and positive charges operating on the opposite surfaces of the membranes can generate energy rich bond between ADP and Pi to synthesize ATPs.
• The basic principle of chemiosmosis is that ion concentration differences and electric-potential differences across membranes are a source of free energy that can be utilized by the cell
• Early evidence supporting a chemi-osmotic mechanism of photosynthetic ATP formation was provided by an experiment carried out by André Jagendorf and co-workers.
Dr. Roshni Rajamohan
Jagendorf’s Experiment
• Andre T. Jogendorf & Earnest Uribe Placedchloroplasts extracted from cells in darkness,thereby eliminating light absorption & electrontransfer as a source of energy forphotosynthesis.
• In the dark, thylakoids were first incubated in amedium of pH 4 until both the exterior andinterior of the vesicles had ph4.
• Then, the thylakoid vesicles were quicklytransferred to a medium with pH 8. At thispoint, there was a pH gradient, with theinterior of the thylakoid (pH 4) having a higherH+ concentration than the exterior (pH 8).
• When ADP was added, ATP was made, even inthe dark. This is convincing evidence linking apH gradient to ATP synthesis.
Dr. Roshni Rajamohan
Dr. Roshni Rajamohan
Efraim Racker
• Efraim Racker- identified and purifiedFactor-1 (F1), the first part of the ATPsynthase enzyme to be characterised.
• F1 is only a part of a larger ATP synthasecomplex. It is a peripheral membraneprotein attached to component Fo, which isintegral to the membrane.
• Racker was able to confirm Peter D.Mitchell's hypothesis that contrary topopular opinion, ATP synthesis was notcoupled to respiration through a high-energy intermediate but instead by atransmembrane proton gradient.
Dr. Roshni Rajamohan
Can a proton gradient drive the synthesis of ATP?
Peter Mitchell’s hypothesis that a proton gradient can drive the
synthesis of ATP was proposed before experimental evidence
supported it and was therefore met with skepticism. In the 1970s,
biochemist Efraim Racker and his collaborator Walther Stoeckenius
tested the hypothesis.
RACKER’S EXPERIMENT Racker and Stoeckenius built an artificial
system consisting of a membrane, a bacterial proton pump activated by
light, and ATP synthase.They measured the concentration of protons in
the external medium and the amount of ATP produced in the presence
and absence of light.
RESULTS In the presence of light, the concentration of protons
increased inside the vesicles, suggesting that protons were taken up by
the vesicles.
In the dark, the concentration of protons returned to the starting level.
ATP was generated in the light, but not in the dark.
INTERPRETATION In the presence of light, the proton pump was
activated and protons were pumped to one side of the membrane,
leading to the formation of a proton gradient. The proton gradient, in
turn, powered synthesis of ATP via ATP synthase.
RACKER’S EXPERIMENT
CONCLUSION: A membrane, proton
gradient, and ATP synthase are sufficient
to synthesize ATP. This result provided
experimental evidence for Mitchell’s
hypothesis.
Dr. Roshni Rajamohan
Boyer’s conformational model (Binding change model)
Our understanding of how ATP is synthesized was advanced by Paul Boyer (1997) at the University of California, Los Angeles, who proposed the binding-change mechanism for catalysis by F-ATPases. From the detailed understanding of how the ATPases function, he proposed that the binding-change mechanism for ATP synthesis contains three important components:
1. The major energy-requiring step is not the synthesis of ATP from ADP and Pi, but the release of ATP from the enzyme.
2. Substrate is bound and products are released at three separate but interacting catalytic sites, corresponding to the three catalytic subunits (β subunits).
3. Each catalytic site can exist in one of three conformations: tight, loose, or open.
The binding changes are coupled to proton transport by rotation of the γ subunit. That is, the flow of protons down their electrochemical gradient through the Fo complex causes the γ subunit to rotate.
Rotation of the γ subunit then brings about the conformational changes in the catalytic complex that allow the release of ATP from the enzyme, and the reaction is driven forward. The reverse occurs when the enzyme functions as an ATP-driven proton pump
Dr. Roshni Rajamohan
Binding change model• In this model, the F1 subunit exists in three states:
1. an O - open - state with very low affinity for substrates and has no catalytic activity;
2. a L - loose - state with low affinity for substrates and also no catalytic activity, and
3. a T - tight - tight state with high affinity for substrates and with catalytic activity.
• The F1 subunit consist of three α and three β subunits, which can cycle between three conformations, bind substrate, and have catalytic activity.
• The collapse of the proton gradient (i.e. the proton-motive force) causes the γ subunit to rotate like a crankshaft relative to the F1 subunit, forcing the β subunit to change conformation from the T to the O (releasing ATP) and then the L (binding ADP and Pi).
• The γ subunit does not appear to undergo any significant conformational change on ATP hydrolysis as evidenced by tritium exchange studies of amide protons.
Top view
Dr. Roshni Rajamohan
The binding-change mechanism as seen from the top of the F1 complex. There are three catalytic sites in three different conformations: loose, open, and tight. (For clarity, only the three β subunits are shown.) Substrate (ADP + Pi) initially binds to the open site and is converted to ATP at the tight site. In step 1, rotation of the γ subunit causes a conformational change, resulting in a change in the formation of the sites. As a result, ATP is released from the enzyme. In step 2, substrate again binds to the open site, and another ATP is synthesized at the tight site. (After Duncan et al. 1995.)
http://6e.plantphys.net/topic12.04.html
Dr. Roshni Rajamohan
The model of Boyer's binding change mechanism. The α and βsubunits configure three nucleotide binding sites: O, which provides theearly binding site for ADP and inorganic phosphate, L, to which the ADPand inorganic phosphate bind after migrating from O, and T, which tightlybinds ATP. Energy ensuing from the movement of protons from thechloroplast lumen to the stroma drives the rotation of the γ subunit of CF1,and the interconversion of the binding sites and the release of an ATPmolecule.
Dr. Roshni Rajamohan
Rotary motion of the γ subunit
• To visualize the rotation of the enzyme, Masasuke Yoshidaand his colleagues at the Tokyo Institute of Technologyattached an actin filament labeled with a fluorescent dye tothe base of the γ subunit using another protein as a "glue."
• They then attached the F1 complex upside down to a glasssurface. If the γ subunit rotates with respect to the catalyticcomplex, the actin filament should swing around with it.Since the filament is very long compared to the ATP synthase(about 1 μm), its rotation should be visible in a fluorescencemicroscope.
• In other words, the fluorescently tagged actin filament,which is large enough to visualize in a light microscope,reports the rotation of the γ subunit.
• When ATP was added to the modified enzyme, the actinfilaments were seen to swing around in a circle at as much as4 revolutions per second in a fluorescence microscope .Demonstration of the rotary motion of the γ subunit made itpossible to put together a model of how the ATP synthaseworks
Dr. Roshni Rajamohan
The γ subunit is inserted like a shaft through the center of the catalytic complex, which consists of three α subunits and three β subunits arranged alternately in a doughnut-like structure. The interface between the γ subunit and the α and β subunits is highly hydrophobic. The hydrophobicity of the interface minimizes the interactions between the subunits, consistent with the rotation of the γ subunit within the hole formed by the catalytic complex. In other words, the γ subunit looks like a molecular bearing lubricated by a hydrophobic interface.
A method for visualizing rotation of the γ subunit. A fluorescently labeled actin filament was attached to one protruding end of the γ subunit. The F1 complex was then attached upside down to a coverslip. When ATP was added to the coverslip, the actin filament rotated. (After Noji et al. 1997.)
Sequential images of the rotating actin filament attached to the γ subunit, as viewed in a fluorescence microscope. There is 133 msbetween the images and the rotation rate is 0.5 r.p.s. (From Noji et al. 1997, courtesy of S. Noji.)
http://docencia.izt.uam.mx/docencia/alva/atpaseyoshida.htm.
Dr. Roshni Rajamohan
Paul D. Boyer
Proposed passage of protons through
the channel of the basal unit makes
the stalk & headpiece spin like a top
just as the flow of water makes a
water wheel turn. The turn cycles
each of 3 catalytic sites on the
headpiece. Through sequential
conformational changes that pickup
ADP & phosphate, combine them, &
release the ATP product. Nobel prize
w/ walker for research into the
mechanisms by which ATP synthase
makes ATP.
John E. Walker
Used X-ray diffraction to create a
3 dimensional picture of ATP
synthase that clearly verified
Boyers model by showing the
head in different rotational
positions as ATP synthesis
proceeds. Nobel prize w/ Boyer
for research into the mechanisms
by which ATP synthase makes
ATP
For their contributions to elucidation of the mechanism of ATP synthesis, Paul Boyer and John Walker shared half the Nobel prize in physiology or medicine in 1997. The other half went to Jens Skou for his pioneering work on the K+,Na+-ATP synthase, the mammalian counterpart of the plant plasma membrane H+-ATP synthase
Dr. Roshni Rajamohan
Suggested Reading:
1. Taiz, L. and Zeiger, E. (2010) Plant Physiology. 5th Edition, Sinauer Associates.
2. William G. Hopkins, Norman P. A. Hüner (2008) Introduction to Plant Physiology, 4th Edition, Wiley.
3. Bhatla, Satish C, Lal Manju A. (2018) Plant Physiology, Development and Metabolism, Springer.