--------------MSSSDSVNNGVNSRMYFRNPSFSN--------------------------VILNDAtERF13 1 --MTADSQSDYAFLESIRRHLLG---ESEPILSESTASSVTQSCVTGQSIKPVYGRNPSFSKLYPCFTEAtERF1 1 MYGQCNIESDYALLESITRHLLGGGGENELRLNESTPS--------------------------SCFTEAtERF2 1 -------------MDPFLIQSPFSGFSPEYSIGSSPDSFS-------------------------SSSSERF1 1 ---------------------------------------------------------------------At3g23220 1 ---------------------------------------------------------------------At5g43410 1 ---------------------------------------------------------------------AtERF14 1--------------MEYQTNFLSGEFSPENSSSSSWSS-----------------------QESFLWEEAt1g06160 1 --------------MEYSQ-------SSMYSSPSSWSSS----------------------QESLLWNEAtERF15 1

NWSDLPLSVDDSQDMAIYNTLRDAVSSGWTPSVPPVTSPAE-----ENKPPATKASGSHAPRQ------AtERF13 30 SWGDLPLKENDSEDMLVYGILNDAFHGGWEPSSSSSDEDRSSFPSVKIETPESFAAVDSVPVKKEKTSPAtERF1 65 SWGGLPLKENDSEDMLVYGLLKDAFHFDT--S-SSDLSCLFDFPAVKVEPTENFTAMEEKPKK-----AAtERF2 44 NNYSLPFNENDSEEMFLYGLIEQSTQQTYIDS-------------DSQDLPIKSVSSRKSE--------ERF1 32 ---------------------------------------------------------------------At3g23220 1 --------------------------------------------------------------MDQGGR-At5g43410 1 --------------------------------------------------------------MDQGGRSAtERF14 1 SFLHQSFDQSFLLSSPTDNYCDDFFAFESS--------------IIKEEGKEATVAAEEEE--------At1g06160 33 SCFLDQSSEPQAFFCPNYDYSDDFFSFESPEM------------MIKEEIQNGDVSNSEEEEK------AtERF15 27

--------KGMQYRGVRRRPWGKFAAEIRDPKKNGARVWLGTYETPEDAAVAYDRAAFQLRGSKAKLNFPAtERF13 88 VSAAVTAAKGKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDRAAFRMRGSRALLNFPAtERF1 134 IPVTETAVKAKHYRGVRQRPWGKFAAEIRDPAKNGARVWLGTFETAEDAALAYDIAAFRMRGSRALLNFPAtERF2 105----------KSYRGVRRRPWGKFAAEIRDSTRNGIRVWLGTFESAEEAALAYDQAAFSMRGSSAILNFSERF1 80 ----------MKYRGVRKRPWGKYAAEIRDSARHGARVWLGTFNTAEDAARAYDRAAFGMRGQRAILNFPAt3g23220 1----GVGAEHGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEEAARAYDQAAYSMRGQAAILNFPAt5g43410 7 SGSGGGGAEQGKYRGVRRRPWGKYAAEIRDSRKHGERVWLGTFDTAEDAARAYDRAAYSMRGKAAILNFPAtERF14 8 ----------KSYRGVRKRPWGKFAAEIRDSTRKGIRVWLGTFDTAEAAALAYDQAAFALKGSLAVLNFPAt1g06160 80 ----VGIDEERSYRGVRKRPWGKFAAEIRDSTRNGIRVWLGTFDKAEEAALAYDQAAFATKGSLATLNFPAtERF15 78

HLIGSCKYEPVRIRPR-------------------------------RRSPEPSVSDQLTSEQKRESHVLRVNSGEPDPVR-----------------------------------IKSKRSSFSSSNENGAP-----LRVNSGEPDPVR-----------------------------------ITSKRSSSSSSSSSSSTSSSENAERVQESLSEIKYTYEDG-------------------------------CSPVVALKRKHSMRRRMTNKHEYQMMKDGPNG-----------------------------------SHENAVASSSSGYRGGGG---GHEYNMGSGV----------------------------------------SSSTAMAGSSSASASA---SHEYNMGTGS----------------------------------------SSTAANS------------SADVVEESLRKMENVNLNDGESPVIALKRKHSMRNRPRGKKKSSSSSTLTSSPSSSSSYSSSSSSSSLSSVEVVRESLKKMENVNLHDGGSPVMALKRKHSLRNRPRGKKRSSSS---SSSSSNSSSCSSSSSTSSTSR

AtERF13 150 AtERF1 204 AtERF2 175

ERF1 140 At3g23220 61 At5g43410 73

AtERF14 78 At1g06160 14

AtERF15 144

DDGESSLVVPELDFTVDQFYFDGSLLMDQSECSYSDNRI---KKRRTVAAGGGMDKGLTVKCEVVEVARGDRLLVL------GKLKRRRKAENLTSEVVQVKCEVGDETRVDELLVS------KTKDSDFDHRSVKLDNVVVFEDLGEQYLEELLGSSENSGTWDDGREVIEFEYLDDSLLEELLDYGERSNQDNCNDANR----SSSRQVFEFEYLDDSVLEELLEEGEKPNKGKK---------SSSQQVFEFEYLDDSVLDELLEYGENYNKTHNINMGKRQ--RSRKQSVVMTQESNTTLVVLEDLGAEYLEELMRSCS-----SSSKQSVVKQESG--TLVVFEDLGAEYLEQLLMSSC-----

AtERF13 188 AtERF1 233 AtERF2 209

ERF1 178At3g23220 92At5g43410 99

AtERF14 95At1g06160 83

AtERF15 210

Supplemental data legend. This alignment of Arabidopsis ERF proteins shown in Figure 1B, wasgenerated by the Multiple Sequence Alignment application (AlignX) of the Vector NTI Suiteprogram (InforMax, Inc.), based on the Clustal W algorithm. Amino acids identical between at leasttwo sequences are shaded.