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Astaxanthin: A Radical Approach to Vitality & Beauty From Within. Natural Products Expo Asia 2005. Vincent Wood PhD, Manager International Business Dept/Marketing. T O D A Y ’ S P R E S E N T A T I O N O U T L I N E. What is Astaxanthin? - PowerPoint PPT Presentation
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NOV2004
Natural Products Expo Asia 2005 Hong Kong
Astaxanthin:Astaxanthin: A Radical Approach to A Radical Approach to Vitality & Beauty From Vitality & Beauty From WithinWithin
Natural Products Expo Asia 2005
Vincent Wood PhD, Manager
International Business Dept/Marketing
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• What is Astaxanthin?What is Astaxanthin?• Why is it so potent compared to other carotenoid Why is it so potent compared to other carotenoid
antioxidants?antioxidants?• The Research & Impact on HealthThe Research & Impact on Health• Summary of Recent AdvancesSummary of Recent Advances
– Supplement use in Muscle Endurance, Eye health and Cosmetic benefitSupplement use in Muscle Endurance, Eye health and Cosmetic benefit
• Retail Examples in JapanRetail Examples in Japan• Regulatory, Production Technology & SupplyRegulatory, Production Technology & Supply
T O D A Y ’ S P R E S E N T A T I O N O U T L I N E
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• 600 known carotenoids*600 known carotenoids*• Humans cannot synthesise Humans cannot synthesise
carotenoids.carotenoids.• Need to obtain carotenoids Need to obtain carotenoids
from food.from food.• <25 obtained from food.<25 obtained from food.• Basic carotene structureBasic carotene structure• Xanthophyll ClassXanthophyll Class
– LuteinLutein– AstaxanthinAstaxanthin
Molecular Formula: Molecular Formula: CC4040HH5252OO44
Molecular Weight:Molecular Weight: 596.82596.82CAS No: CAS No: 472-61-7472-61-7
O
HO
O
OH
*Parker RS.,1997
A M E M B E R O F T H E C A R O T E N O I D F A M I L Y
AstaxanthinAstaxanthin
LuteinLutein
-Carotene-CaroteneOH
OH
NOV2004
Natural Products Expo Asia 2005 Hong Kong
1938 1938 Lobster Lobster (Astacus gammarus) Discovered(Astacus gammarus) Discoveredby Nobel Laureate Dr Kuhnby Nobel Laureate Dr Kuhn
Present Day Present Day Other SourcesOther SourcesMicroalgae Microalgae (Haematococcus pluvialis)(Haematococcus pluvialis)Yeast Yeast (Phaffia rhodozyma)(Phaffia rhodozyma)BacteriaBacteria (Agrobacterium aurantiacum)(Agrobacterium aurantiacum)Higher PlantsHigher Plants (Adonis aestivalis)(Adonis aestivalis)Crustaceans Crustaceans ((Euphasia supurbaEuphasia supurba))Fish Fish (Salmon) Typically 1mg per 200g(Salmon) Typically 1mg per 200gBirds Birds (Flamingo, Cockerel)(Flamingo, Cockerel)
A S T A X A N T H I N B A C K G R O U N D
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Haematococcus pluvialisHaematococcus pluvialis
AstaxanthinAstaxanthinPowerful Antioxidant Powerful Antioxidant DDefenefencce Against Free Radicalse Against Free RadicalsPProtects Plant Cell Contentsrotects Plant Cell Contents
HaematoHaemato (Blood) (Blood) coccuscoccus (Seed) (Seed)
Microalgae family – includes Microalgae family – includes SpirulinaSpirulina
Harsh Environment – Dormant / HibernatesHarsh Environment – Dormant / Hibernates
““Astaxanthin efficiently functions as an Astaxanthin efficiently functions as an antioxidant agent in both cytoplasmic and antioxidant agent in both cytoplasmic and membraneous regions to protect cells in membraneous regions to protect cells in vivo against environmental oxidative vivo against environmental oxidative stress.”stress.”(1999 Kobayashi, M., Okada, T. Protective Role of Astaxanthin to (1999 Kobayashi, M., Okada, T. Protective Role of Astaxanthin to Protect Against UVB Radiation in the Green Alga Haematococcus Protect Against UVB Radiation in the Green Alga Haematococcus pluvialis.)pluvialis.)
Microalgae 15,000 ppm Yeast 8,000 ppm Krill 1,500 ppm.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
A S T A X A N T H I N M E A N S M O R E H E N S F O R T H E R O O S T E R !
COME HERE COME HERE GIRLS!GIRLS!
NOV2004
Natural Products Expo Asia 2005 Hong Kong
A S T A X A N T H I N & C A R O T E N O I D P E R C E P T I O N S
Low level of consumer and retail awareness
Too many antioxidants on Too many antioxidants on the marketthe market
Contradictory carotenoid evidence. Not enough science-carotene>lycopene>lutein>astaxanthin
A functional based product is A functional based product is being developed based on being developed based on microalgal astaxanthinmicroalgal astaxanthin
Natural vs Synthetic
Microalgae vs Yeast
Perceived to be expensive!
only small amounts are only small amounts are required. Can complements required. Can complements existing formulations existing formulations
Unstable supply in the past,
Intense competition
‘‘Wait and see” attitude on Wait and see” attitude on market developmentsmarket developments
“Frost & Sullivan estimates that astaxanthin is now one of the fastest growing carotenoids in Europe. European carotenoids were worth $348.5 million in 2003, with 17 per cent of this coming from supplements. But currently less than 1 ton of astaxanthin is going into supplements per year, compared to up to 150 tons for the aquaculture industry.” Source: Nutraingredients.com 22/03/04
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Physical QuenchingPhysical Quenching 1O2 + 1Carotenoid → 3O2 + 3Carotenoid* 3Carotenoid* → 1Carotenoid + thermal energy*
↓
ASTAXANTHIN ELIMINATES FREE RADICALS EXTREMELY FAST BY ENERGY TRANSFER**
Chemical Reaction Chemical Reaction (e.g. (e.g. -Carotene)-Carotene)
1O2 + Carotenoid → Carotenoid RADICAL → OXIDIZED*B.R.Nielsen, K.Jorgensen and L.H.Skibsted : Triplet-triplet extinction coefficients, rate constants of triplet decay and rate constants of anthracene triplet sensitization by laser flash photolysis of astaxanthin, β-carotene, canthaxanthin and zeaxanthin in deaerated toluene at 298K, J.Photochem.Photobio.A:Chem., 112, 127-133 (1998).
A N T I O X I D A N T M E C H A N I S M S
“The energy of the singlet state oxygen is quickly transferred to the carotenoid by direct contact, and the carotenoid in the triplet state relaxes to the singlet state very quickly by the transformation of the potential energy into thermal energy through the vibration of conjugated double bonds in the polyene chain.” (Miki, M., Biological Functions and Activities of Animal Carotenoids. Pure Appl. Chem. 63. 141-146. 1991.)
NOV2004
Natural Products Expo Asia 2005 Hong Kong
0
500
1000
1500
2000
2500
3000
3500
Astaxanthin Zeaxanthin Cantaxanthin Lutein ß-Carotene Vitamin E
Antioxidant
ED
50 v
alu
e (nM
)
Scavenging: Carotenoids with oxygen groups most efficient against lipid peroxidation.
Astaxanthin was the most efficient scavenger. Miki, W. Pure & Appl. Chem. 63(1);141-46
S C A V E N G I N G E F F E C T O F D I F F E R E N T A N T I O X I D A N T S I N A L I P O P H I L L I C
I N V I T R O M O D E L
NOV2004
Natural Products Expo Asia 2005 Hong Kong
e - e -
3 O2 → ・ O2- → H2O2 → ・ OH O 2 LH
1 O2 LH → L ・ → LOO ・ → LOOH
H2O L ・
SODCatalase Ascorbic Acid
Astaxan
thin
↓ ↓ ↓ ↓
↓ ↓
QUENCHINGQUENCHING550 x550 x Vitamin E* Vitamin E*40 x 40 x -carotene*-carotene*
INHIBITSINHIBITSPEROXIDATIONPEROXIDATION
1000 x Vitamin E**1000 x Vitamin E**
N E U T RA L I Z I N G R O S
(Superoxide radical)
(Singlet oxygen radical)
(Hydroxy radicals)
(Peroxide radicals)
*N.Shimizu et al.: Carotenoids as singlet oxygen quenchers in marine organisms, Fisheries Science, 62, 134-137 (1996).
**Miki, W. Pure & Appl. Chem. 63(1);141-46. (ROS: Reactive Oxygen Species)
Astaxanthin
Vitamin E(tocopherol)
NOV2004
Natural Products Expo Asia 2005 Hong Kong
0 1 2 3 4
Astaxanthin
Zeaxanthin
Lycopene
β-Carotene
Normalized relative oxidation rates
H.D.Martin, et al. : Chemistry of carotenoid oxidation and free radical reactions,
Pure Appl. Chem., 71(12), 2253-2262 (1999).
Pure Pure Anti-oxidantAnti-oxidant
Anti- andAnti- andPro-oxidantsPro-oxidants
A S T A X A N T H I N - I S T H E P U R E A N T I O X I D A N T
NOV2004
Natural Products Expo Asia 2005 Hong Kong
T H E A S T A X A N T H I N S H I E L D
Lignell A., & Wood V., Astaxanthin: Radical Cellular Defense. May 2004 Innovations in Food Technology.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
A S T A X A N T H I N D I S T R I B U T I O N I N R A T S A F T E R 3 W E E K S O N S U P P L E M E N T E D D I E T
( 1 0 0 m g A x / k g f e e d ).
0
10
20
30
40
50
60
70
80
Blood Brain Heart Kidney Liver Lung Muscle Skin
Organ
µg a
stax
anth
in/k
g
A S T A X A N T H I N S H I E L DA S T A X A N T H I N S H I E L D
US Pat 6,245,818 Medicament for improvement of duration of muscle function or treatment of muscle disorders or diseases.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
P R O P E R T I E S O F A S T A X A N T H I N
Antioxidant Potency
Powerful Powerful singlet oxygensinglet oxygen quenching quenching and and free radicalfree radical scavenging activity. scavenging activity.
Protects against Protects against lipoperoxidationlipoperoxidation..
Solubility
Spans the membrane bi-lipid layer – Spans the membrane bi-lipid layer – comprehensive protection and comprehensive protection and strengthens rigiditystrengthens rigidity
Protection Forms a Forms a protective shieldprotective shield and and defends against continuous free defends against continuous free radical attack at the cellular level.radical attack at the cellular level.
Safety Defined as a Defined as a pure antioxidantpure antioxidant unlike unlike other well known antioxidants that can other well known antioxidants that can become become pro-oxidantspro-oxidants..
NOV2004
Natural Products Expo Asia 2005 Hong Kong
C A T E R G O R I Z E D L I T E R A T U R E
C A T E R G O R Y P U B L I C A T I O N SGeneral NutritionGeneral Nutrition
Stomach & Gut Health*Stomach & Gut Health*
Eye Health**Eye Health**
Sports Nutrition*Sports Nutrition*
Vetinary Health*Vetinary Health*
CosmecuticalsCosmecuticals
Antioxidant potency, Synergies.Antioxidant potency, Synergies.
Helicobacter, dyspepsiaHelicobacter, dyspepsia
Visual Fatigue, Diabetic CataractVisual Fatigue, Diabetic Cataract
Muscle Endurance and damage, Muscle Endurance and damage, Visual Acuity, anti-inflammatory. Visual Acuity, anti-inflammatory.
Fertility, muscle endurance, anti-Fertility, muscle endurance, anti-inflammatoryinflammatory
UVR damage, photoprotectant, UVR damage, photoprotectant,
anti-inflammatory. anti-inflammatory.
*Patent Protected **Patent Applied
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Sports nutrition & supplement– Major market potential. Continues positive growth.
• Strenuous physical activity generates free radicals– up to 12 times more than at rest
• Activation of the inflammation response– Important factor for post strenuous exercise & recovery
• Feel oxidative damage – Muscle pain, tiredness, need for recovery
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Protocol40 young healthy male students, age 17 - 19, were included. Standardized exercise tests were carried out at baseline and after three and six months of supplementation with 4 mg astaxanthin per day or placebo.
Inclusion 4 mg astaxanthinRandomisation Placebo
Parameters measured:- Fitness- Strength / endurance- Strength / explosivity
3 months 3 months
Dietary supplementation with astaxanthin improves muscle endurance.
A randomised double blind placebo controlled study on healthy male subjects.
(In Press) Investigator Curt L. Malmsten, PhD, MD,
Räddningshälsan AB,
Sweden.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
R E S U L T S : C H A N G E S I N S T R E N G T H / E N D U R A N C E
Baseline
30
35
40
45
50
55
60
65
70
75
80
1 2 3
No.
of k
nee
bend
ings
/per
son
Placebo
Astaxanthin
6 months3 monthsBaseline
p < 0.05
Significant increase of muscle endurance.
No difference in fittness.
No difference in muscle explosivity.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Astaxanthin may be effective for:
Endurance Levels (4 mg daily dose)
Maintains Antioxidant Protection Levels
Reduces Recovery Time to Training
2002 Sawaki K., et al., reported a 28.5%
(P<0.05%) reduction in lactic acid build up
after 1200m supplemented subjects.
On-going US and Swedish clinical trials.
Results Due 1st QTR 2005.Muscle endurance US Pat 6,245,818
C O N C L U S I O N S
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Damage MarkersMuscle cell staining – Cell membrane damageCreatine kinase (CK) – Damaged muscle cells8-hydroxy-2’-deoxyguanosine (OHdG) – DNA damage
Myeloperoxidase (MPO) - Inflammation
Acclimatization
・ Control (N=8)・ Exercise Control (N=8)・ Exercise Supplemented (N=8)
Training Program
Exercise
Protocol
1 wk 3wk
Euthanized
24hr
Astaxanthin Limits Exercise-Induced Skeletal and Cardiac Muscle Damage in Mice
Aoi, W. et al. 2003, Antioxidants & Redox Signalling, 5:(1) 139-144.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
M U S C L E C E L L M E M B R A N E
Gastrocnemius
Heart
CONTROL EXERCISE CONTROL EXERCISE + AX
CONTROL EXERCISE + AXEXERCISE CONTROL
NOV2004
Natural Products Expo Asia 2005 Hong Kong
R E S U LT S
Creatine Kinase in Plasma
0
500
1000
1500
2000
2500
3000
3500
Control Exercised Ax+Exercised
U/L
p<0.05 p<0.05
Inflammation in Gastrocnemius and Heart
0.00
0.50
1.00
1.50
2.00
2.50
3.00
Control Exercised Ax+Exercised Control Exercised Ax+Exercised
Gastrocnemius Heart
MP
O A
cti
vit
y (
mU
/mg
pro
tein
)
*P<0.05*P<0.05*P<0.05*P<0.05
Production of 8-OHdG (DNA Damage)
0.00
0.50
1.00
1.50
Control Exercised Ax+ Exercised Control Exercised Ax+Exercised
Gastrocnemius Heart
8-O
Hd
G/1
05 x
dG
p< 0.05 p< 0.05
Overall Reduction of DNA damage
Reduction of Inflammatory Response
Reduction of Muscle Cell Damage
New Research to Mechanism using Gene Chip Analysis - 2005
NOV2004
Natural Products Expo Asia 2005 Hong Kong
I N F L A M M A T O R Y R E S P O N S E – I N A C T I V A T I O N OF N F – K B
Lignell A., & Wood V., Astaxanthin: Radical Cellular Defense. May 2004 Innovations in Food Technology.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Visual Display Terminals (VDT) at work and leisure. Increased Risk of developing glaucoma for heavy
computer use combined with short sightedness*. Eye Disorders – Blurring, Occular & Visual Asthenopia,
Headache Visual Acuity, Accommodation, Critical flicker fusion, Depth
Perception, Pattern Evoked Potential Deteriorates on Daily Basis & Fatigue Accumulates Age Related Macular Degeneration** No Data on Astaxanthin on VDT Induced Visual Fatigue
*Source: BBC News 16 Nov 2004 Japanese research 10,000 Japanese workers: Computer link to eye disease.
**US Patent 5,527,533, Tso & Lam, “Method of Retarding and Ameliorating Central Nervous System and Eye Damage”
E Y E H E A L T H - F A T I G U E
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Visual Acuity (Landolt Ring) 5m & 35 cm
Accommodation Amplitude = Ciliary Muscle Function
Critical Flicker Fusion (CFF) = Central Nervous System (CNS)
Pattern Evoked Potention (PEVP) = Visual Nerve connection the to brain
Patient Questionnaire (Subject monitoring devised by Ophthalmologists)
C L I N I C A L P A R A M E T E R S
*Nagaki Y., et al., 2002 Journal of Traditional Medicines 19(5):170-173.**Koide R., et al., 1994 Effects of Whortleberry extract on ocular function. J. Eye 11:117-121.***Sawaki K., et al., 2002. Sports Performance Benefits from Taking Natural Astaxanthin Characterized by Visual Acuity and Muscle Fatigue in Humans. J. Clin. Ther. Med. 18(9):73-88.****Nakamura A., et al., 2004. Changes in Visual Function Following Peroral Astaxanthin. 57th Congress of Clinical Opthalmology of Japan 58(6):1051-1054 (Japanese).
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Questionnaire revealed a 54% reduction of eye strain symptoms* at 5 mg/day after 4 weeks (N=26) and better accomodation (P<0.01). Agree with Whortleberry** Hypofunction of Ciliary Body
6mg/day*** for 4 weeks (n=18) showed better depth perception and CFF (P<0..05)
Accomodation improved at 4 mg (P<0.05) and 12 mg/day (P<0.1) (n=49)****
FOSHU Planned Mid 2005 Agent for improving
accommodation blood flow in the eye. VDT Subjects w ith Eye Strain Symptoms before and after astaxanthin supplementation
0
2
4
6
8
10
12
14
Before After Before After
Group B (Treated) Group C (Placebo)
No
. o
f S
ub
jects
wit
h E
ye
Str
ain
E Y E F A T I G U E C O N C L U S I O N
*Nagaki Y., et al., 2002 Journal of Traditional Medicines 19(5):170-173.**Koide R., et al., 1994 Effects of Whortleberry extract on ocular function. J. Eye 11:117-121.***Sawaki K., et al., 2002. Sports Performance Benefits from Taking Natural Astaxanthin Characterized by Visual Acuity and Muscle Fatigue in Humans. J. Clin. Ther. Med. 18(9):73-88.****Nakamura A., et al., 2004. Changes in Visual Function Following Peroral Astaxanthin. 58(6):1051-1054 (Japanese).
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Wrinkles - where do you have them?
Eyes>Forehead>Mouth
UNAVOIDABLE. It’s all part of the aging process.
Causes of aging can be partially counteracted.
By improving nutrition and minimizing phototic injury.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Astaxanthin
BEAUTY FROM WITHIN – The Next Hot Market SegmentL’Oreal & Nestle – “Innévo” 2002 Targeting women >40 y.o.***
Early studies in mice models revealed significant long terms protection against premature wrinkle formation caused by UVA and UVB exposure*
Topical application lowers the UVB induced inflammatory process that may lead to hyperpigmentation**
*Arakane K., 2002. Superior protection via astaxanthin. Carotenoid Science, Vol 5.
**Yamashita E., 1995. Suppression of Post UVB hyperpigmentation by Topical astaxanthin from krill. Fragrance Journal 14:180-185. ***Times, March 2004 (US) L’Oreal innovations - Beauty foods.
NOV2004
Natural Products Expo Asia 2005 Hong Kong
Cosmetic benefit of a Dietary Supplement Cosmetic benefit of a Dietary Supplement Containing Astaxanthin & TocotrienolContaining Astaxanthin & Tocotrienolon Human Skinon Human Skin
Yamashita, E. 2002, FOOD Style 21, 6:(6) 112-117.
A Randomized Double Blind Placebo Controlled Study
Supplemented group - Astaxanthin 2mg from Haematococcus pluvialis - Tocotrienol 40mg from palm fruit - Canola oilSubjects
- Healthy female with dry skin conditions
- n=8 (supplemented group) + 8 (placebo)
- Mean age: 40 years old
Methods
- 1 capsule per day in the evening for 4 weeks
- Parameters measured at start, 2 and 4 weeks
Parameters
- Standardized questionnaire
- Dermatologist inspection/palpation
- Skin moisture & sebum content
- Magnified skin surface inspection
NOV2004
Natural Products Expo Asia 2005 Hong Kong
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Skin condition by individual assessment
R E S U L T S 1R E S U L T S 1
4 Weeks
-1.0
0.0
1.0
2.0
3.0
Treated
Placebo2 Weeks
-1.0
0.0
1.0
2.0
3.0
Treated
Placebo
To
tal
Sag
Po
uc
h
Fin
e w
rin
kle
Wri
nkl
e
Red
nes
s
Sp
ots
/Fre
ckle
s
Du
lln
ess
Acn
e/P
imp
les
Co
mp
lexi
on
Ela
stic
ity
Oil
ines
s
Sm
oo
thn
ess
Mo
istu
re
To
tal
Sag
Po
uc
h
Fin
e w
rin
kle
Wri
nkl
e
Red
nes
s
Sp
ots
/Fre
ckle
s
Du
lln
ess
Acn
e/P
imp
les
Co
mp
lexi
on
Ela
stic
ity
Oil
ines
s
Sm
oo
thn
ess
Mo
istu
re
Ch
ang
es o
f sk
in c
on
dit
ion
(-w
ors
e/+
bet
ter)
Ch
ang
es o
f sk
in c
on
dit
ion
(-w
ors
e/+
bet
ter)
p<0.05 p<0.05
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Dermatologist inspection/palpation
R E S U L T S 2R E S U L T S 2
-3
-2
-1
0
1
2
3
Traeted
PlaceboWeek 2
To
ug
hE
last
icit
y
Sm
oo
thn
ess
Sag
Po
uc
h
Fin
e w
rin
kle
Wri
nkl
e
Red
nes
s
Sp
ots
/Fre
ckle
sD
ull
nes
s
Acn
e/P
imp
les
Co
mp
lexi
on
Oil
ines
s
Ski
n t
ext
ure
Mo
istu
reP
ulp
free
Dar
k ri
ng
s ar
ou
nd
eye
s
Ski
n c
olo
r
-3
-2
-1
0
1
2
3
Treated
PlaceboWeek 4
Sm
oo
thn
ess
Sag
Po
uc
h
Fin
e w
rin
kle
Wri
nkl
e
Red
nes
s
Sp
ots
/Fre
ckle
sD
ull
nes
s
Acn
e/P
imp
les
Co
mp
lexi
on
Oil
ines
s
Ski
n t
ext
ure
Mo
istu
re
To
ug
h
Pu
lpfr
ee
Dar
k ri
ng
s ar
ou
nd
eye
s
Ski
n c
olo
r
Ela
stic
ity
p<0.05
p<0.01p<0.1
p<0.1p<0.1
Ch
ang
es o
f sk
in c
on
dit
ion
(-w
ors
e/+
bet
ter)
Ch
ang
es o
f sk
in c
on
dit
ion
(-w
ors
e/+
bet
ter)
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Skin moisture content
Eye Region
-20
-10
0
10
20
30
40
50
2 Weeks 4 WeeksVaria
tion
(mic
roS)
Treated
PlaceboCheek
-20
-15
-10
-5
0
5
10
15
2 Weeks 4 Weeks
Var
iation
(m
icro
S)
Treated
Placebo
R E S U L T S 3R E S U L T S 3
p<0.05
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Skin sebum content
Brow
-12
-10
-8
-6
-4
-2
0
2
4
6
2 Weeks 4 Weeks
Var
iation
TreatedPlacebo Cheek
-12
-10
-8
-6
-4
-2
0
2
4
6
2 Weeks 4 Weeks
Var
iation
TreatedPlacebo
R E S U L T S 4R E S U L T S 4
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Magnified skin surface inspection
R E S U L T S 5R E S U L T S 5
M4
4 weeks2 weeksStart
NOV2004
Natural Products Expo Asia 2005 Hong KongPossible Mechanism of ActionPossible Mechanism of Action
- Visual Wrinkle Reduction -- Visual Wrinkle Reduction -
1O21O2
1O2
UV
A
UV
A
Singlet oxygen induced UVA oxidizes collagen→ WRINKLE
AstaxanthinAstaxanthin
AstaxanthinAstaxanthin
New CollagenNew CollagenThank you !!Thank you !!
TocotrienolTocotrienol
TocotrienolTocotrienol
NOV2004
Natural Products Expo Asia 2005 Hong Kong
• Increased moisture levelsIncreased moisture levels• Consistent natural oilsConsistent natural oils• Reduction of fine wrinkles and pimplesReduction of fine wrinkles and pimples• Improved elasticityImproved elasticity• Reduced swelling under the eyesReduced swelling under the eyes
Astaxanthin & Tocotrienol supplementation resulted in
C O N C L U S I O N C O N C L U S I O N
NOV2004
Natural Products Expo Asia 2005 Hong Kong
J A P A N E S E R E T A I L P R O D U C T S
Astaxanthin & Blueberry
Astaxanthin & Natto Kinase
Astaxanthin + Astaxanthin + Tocotrienol & SOD Tocotrienol & SOD 60 soft cap 60 soft cap
3,800 JPY/month3,800 JPY/month
37 USD or 287 HKD37 USD or 287 HKD
Pure Astaxanthin
Astaxanthin Astaxanthin 1mg/day + 1mg/day + DHA+ARADHA+ARA
6,300 JPY/month6,300 JPY/month
61 USD or 477 HKD61 USD or 477 HKD
Astaxanthin 2mg/day+ pomegranate juice Astaxanthin 2mg/day+ pomegranate juice 500mL. 6,000 JPY/month, 58 USD or 453 HKD500mL. 6,000 JPY/month, 58 USD or 453 HKD
NOV2004
Natural Products Expo Asia 2005 Hong Kong
C O S M E C E U T I C A L S & B E A U T Y S U P P L E M E N T S
NatuReal Corp.NatuReal Corp.
Astaxanthin, Tocotrienol & Astaxanthin, Tocotrienol & CollagenCollagen
Supplement 7,000 JPY/monthSupplement 7,000 JPY/month
Cream 9000 JPY Cream 9000 JPY
Essence 10,000 JPY Essence 10,000 JPY
68-97 USD or 529-755 HKD68-97 USD or 529-755 HKD
J A P A N E S E R E T A I L P R O D U C T S
Shiseido: Pure White Shiseido: Pure White Astaxanthin 0.5 mg/day plus Astaxanthin 0.5 mg/day plus multivitamins. multivitamins.
Drink 250 JPY 250 mL/day, Drink 250 JPY 250 mL/day, 2.40 USD or 18.9 HKD2.40 USD or 18.9 HKD
Tablet 4000 JPY/ month,Tablet 4000 JPY/ month,
39 USD or 302 HKD39 USD or 302 HKD
NOV2004
Natural Products Expo Asia 2005 Hong Kong
R E G U L A T O R Y I N F O R M A T I O N
US DSHEA granted for 2 mg/day – 4 DSHEA granted for 2 mg/day – 4 mg/day. mg/day.
No GRAS statusNo GRAS status
CFTA/INCI ListedCFTA/INCI Listed
Japan Listed under Food Additives & Listed under Food Additives & ColouringColouring
JP Cosmetic ListedJP Cosmetic Listed
Europe Only biomass approvedOnly biomass approved
EU approvals not yet harmonisedEU approvals not yet harmonised
Requires EU Novel Foods Requires EU Novel Foods Approval Approval
Country SpecificCountry Specific
China/Hong Kong Rquires “Bao Jian Shipin” or Rquires “Bao Jian Shipin” or Health Enchaning Foods approvalHealth Enchaning Foods approval
No information availableNo information available
NOV2004
Natural Products Expo Asia 2005 Hong Kong
I N T E R N A T I O N A L M A R K E T I N G S E C T I O NI N T E R N A T I O N A L M A R K E T I N G S E C T I O N
33rdrd F L R H A L I F A X O N A R I M O N B L D. F L R H A L I F A X O N A R I M O N B L D.
3 – 2 4 –1 0 N I S H I S H I N B A S H I 3 – 2 4 –1 0 N I S H I S H I N B A S H I
M I N A T O – K U M I N A T O – K U
T O K Y O 1 0 5 – 0 0 0 3 J A P A NT O K Y O 1 0 5 – 0 0 0 3 J A P A N
( T ) + 8 1 – 3 – 3 4 3 7 – 2 3 5 3 ( T ) + 8 1 – 3 – 3 4 3 7 – 2 3 5 3
( F ) + 8 1 – 3 – 3 4 3 7 – 2 3 4 7( F ) + 8 1 – 3 – 3 4 3 7 – 2 3 4 7
w w w . f u j i c h e m i c a l . c o . j p / e n g l i s hw w w . f u j i c h e m i c a l . c o . j p / e n g l i s h
E m a i l : i n f o @ f u j i c h e m i c a l . c o . j pE m a i l : i n f o @ f u j i c h e m i c a l . c o . j p
C O N T A C T
Thank you for your kind attention!
For more information vist Booth E17