Assisted Reproductive Technologies...Assisted Reproductive Technologies LIFE. Cryo Bio System is exclusively dedicated to human life sciences. It provides innovative solutions for

AssistedReproductiveTechnologies

LIFE

mandy.jansz

Sci-NZ

Cryo Bio System is exclusively dedicated to human life sciences. It provides innovative solutions for long-term cryopreservation of biological samples to a varied scientific community through a range of high security products for:

• Assisted Reproductive Technologies

• Biobanks involved in epidemiological research and clin-ical studies;

• Blood donation center sample banks;

• Cellular and gene therapy units;

• All other medical, pharmaceutical and research applica-tions which use cryopreservation for biological samples.

Cryo Bio System works in more than 70 countries through its 5 subsidiaries (Italy, the Netherlands, USA, India and China) and a network of highly effective distributors. It focuses resolutely on its products’ quality and takes care to stay close to customers, ensuring it meets the needs of professionals working in assisted reproduction.

Our comprehensive range of products for use with human samples allows sperm, em-bryos and gonadal tissue to be frozen at very low temperatures, as well as permitting vitri-fication of oocytes and embryos at various stages of development. Our unique concept of High Security long-term cryopreservation guarantees unparalleled security and quality when storing any kind of biological sample.

Cryo Bio System was founded in 1987 and is a division of the IMV Technologies group, a world leader in animal reproduction biotechnology based in L’Aigle (Normandy, France).

C O N T E N T S IVF

Recisperme 4 Isolating transport container 4 Leja counting chambers 5 Makler® counting chamber 6 Aspiglaire 6 Craft™ Pump 6 ICSI, holding and biopsy pipettes 7 HIGH SECURITY VITRIFICATION

HSVTM High Security Vitrification straw 8

CRYOPRESERVATION CBS™ High Security Tube 10 CBS™ High Security embryo and sperm straw with tripartite plug 11 CBS™ High Security sperm straw with tripartite plug 12 Sterile filling nozzle for CBS™ straws 14 CBS™ High Security tissue 2 mL straw 14 PETG sperm straw 15 PETG embryo straw 15 ACCESSORIES BMP 21 and BMP 51 printer 16 Micro-aspirator 16 Canisters 17 CBS™ visotubes and goblets 18

EQUIPMENT SYMS III 20 SYMS 21 PACE 22 MAPI 23 NANO-Digitcool controlled rate freezer 24 SYSO 25 Automatic sperm collector 26NEW!

4

Isolating transport container

RecispermeSINGLE USE STERILE SPERM COLLECTION VESSEL

DESCRIPTION / USESpermogram, sperm culture, spermocytogram (insemination, in vitro fertilization, diagnosis).Sterilization performed by irradiation.Individually packaged. Box of 20 units.

TECHNICAL SPECIFICATIONSThe innovative design of the Cryo Bio System Recisperme allows sperm to be collected directly into a tube, eliminating the need for sample transfer and enhancing sample qual-ity by avoiding tricky handling.

Preassembled sterile unit comprised of:• a funnel-shaped upper part;• a 10 mL tube with blue stopper;• a support base; and• an adhesive cover.

INSTRUCTIONS FOR USE• Once it has been labelled with the patient’s

name, weigh the device (the support base, tube and upper part or the support base, tube and connected blue stopper).

• After ejaculation directly into the vessel, the adhesive cover can be applied to guarantee that the sample remains sterile;

• Check that all the sperm has travelled from the funnel-shaped upper part to the tube;

• Then disconnect the upper part and adhesive cover from the tube and close the tube using the blue stopper.

• Weigh the device using the same configu-ration as was used to weight it before use in order to measure ejaculate volume using a sperm density of 1g/mL (WHO laboratory manual for the Examination and process-ing of human semen - Fifth edition).

• Remove the support and funnel-shaped upper part and dispose of them according to biomedical waste procedures.

SHELF-LIFEDo not use the device once the year, month or day indicated has passed.

007426 Box of 20 Recispermes

INSTRUCTIONS FOR USE• Open the container by opening up the lid.• Place the tube into the opening situated in

the middle of the container.• Close the container by putting the lid

back on.

The container is now ready to be transport-ed to the clinic for insemination.

If necessary, the container can be labelled for identification.Maximum duration of transportation: 15 minutes.

DESCRIPTION / USEFor transportation of prepared sperm prior to intrauterine insemination.High density polyethylene container with polyurethane foam insert precut in the mid-dle to hold one tube.All commercially available 5 to 15 mL round or conical bottom culture tubes fit the size of the precut holder.

TECHNICAL SPECIFICATIONSLength: 120 mmDiameter: 65 mm

Two hours prior to use, the container can be placed in a laboratory incubator at 37°C. This ensures that a tube filled with prepared sperm is at an optimal temperature for trans-portation from the laboratory to the clinic where the insemination will be performed.The container guarantees sperm mobility by avoiding thermal shock.

015937 Bag of 5 isolating transport containers

CE marked

IVF

Assisted Reproduction Technologies5

LEJA counting chamber

DESCRIPTION / USEHigh quality disposable counting chambers for semen analysis. Due to their special character-istics, Leja slides both save time and increase the level of accuracy and precision for semen analysis.All slides are covered with a special coating to prevent air bubble formation and to prevent semen from sticking to the chamber surface. The resin and ink used are both non-toxic.The Leja slides have an excellent low limit of quantification and can handle high sperm concentrations. This has a direct clinical impact: You will be better prepared to determine the thresholds for the various Assisted Reproduction Technologies methods (waiting for natural con-ception, intrauterine insemination, in-vitro fertil-ization or intracytoplasmic sperm injection) and to decide which treatment is best for a couple. The high level of accuracy and precision of the Leja counting chambers will allow your results to be compared with other laboratories’ scientific data concerning the relationship between semen parameters (concentration, motility, viscosity) and fertility.

TECHNICAL SPECIFICATIONSSlides with 2, 4 or 8 chambersDepth: 10, 12, 20 or 100 microns

025226 Leja slides SC-10-01-04-B 10μm4wells–4x25

026351 Leja slides SC-12-01-C 12μm2lateralwells–4x25

026352 Leja slides SC-20-01-C 20μm2lateralwells–4x25


026354 Leja slides SC-20-01-02-B CE 20μm2IVDwells–4x25


026355 Leja slides SC-20-01-04-B CE 20μm4IVDwells–4x25


026356 Leja slides SC-100-01-02-A 100μm2highprecisionwells - 4 x 25

026357 Leja slides SC-100-01-02-A CE 100μm2highprecisionIVDwells - 4 x 25


026351

025226 025108

026356 - 026357 - 026043

026352

026353 - 026354

025107 - 026355

6

IVF

Makler® counting chamberDESCRIPTION / USERapid sperm analysis.Counting chamber and cover glass for counting and analysis of spermatozoa.

TECHNICAL SPECIFICATIONSReusable: Easy to clean with a bleach-free disinfectant.The 10-micron depth eliminates blurring and allows sperm to move freely. The applied sample is observed in one focal plane. The grid is positioned on the cover glass. The grid therefore does not need to be placed under or retrieved from a microscope.

INSTRUCTIONS FOR USEEasy-to-use: sperm count performed on undiluted specimen.The number of spermatozoa is count-ed within the 10 squares of 0.1 mm x 0.1 mm. Multiply this number by 1 mil-lion to obtain the quantity of sperm per milliliter.No additional factors are necessary for calculation.Observation of color fringes at the four contact points provides a self-controlled test for accuracy. The cover glass can never be contaminated by the sample.The device can be used multiple times without losing precision or requiring fur-ther calibration.

016261 Makler®countingchamber (completewithcoverglassandgrid)

016262 Sparecoverglasswithgrid

Aspiglaire

Craft™ pump

ENDOCERVICAL ASPIRATOR FOR HÜHNER TESTS

DESCRIPTION / USEHühner test (post-coital sample collection).Mucus viscosity evaluations.Endo- or exocervical mucus sampling.Sterilization performed by irradiation.Individually packaged or in peelable bags of 5 units. Box of 20 or 40 units.

TECHNICAL SPECIFICATIONSThe Aspiglaire catheter has two components:• a clear, flexible sheath with a round, atraumatic

tip; and• an inner blue plunger.

No syringe needed.Length: 280 mmExternal diameter: 2.92 mm

007447 Boxof20Aspiglaires(individuallypackaged)007448 Boxof40Aspiglaires(8bagsof5units)

005553 Craft™pump–Rocketvacuumpump(comeswithaccessories)015621 PedalforCraft™pump006553 Craft™pumptubing018204 Craft™pumpreservoir018268 CapforCraft™pumpreservoir019270 Luer-LockadaptorforCraft™pumptubing

DESCRIPTION / USEOocyte and follicular fluid aspiration.

The Craft™ pump is specifically designed to provide a smooth low volume vacuum at a predetermined negative pressure, in order to collect oocytes through follicular aspiration.

12-month warranty.

TECHNICAL SPECIFICATIONSThe clinician collecting oocytes operates the aspirator via a pedal.

L x W x H: 20 x 14 x 12 cmWeight: 3.2 kgVacuum: 0 - 400 mm Hg

CE marked

INSTRUCTIONS FOR USE• Slowly insert the Aspiglaire into the cervical canal

until reaching the endocervical sampling site.• Aspirate the cervical mucus by pulling the plunger

to draw it into the tube. The plunger automatically stays in the desired position.

• Gently withdraw the Aspiglaire from the patient’s body.

• Apply the sample to a slide for examination.


CE marked


ICSI, holding and biopsy pipettes

DESCRIPTION / USEICSI injection pipettes are used to perform intracytoplasmic sperm injections (ICSI).

TECHNICAL SPECIFICATIONSThe unique shape and taper of our ICSI pipettes provide for smooth movement of its content.A few sperm can be loaded for injecting a few oocytes at one time. The short, sharp, fine spike at the tip allows for easy, atraumatic penetration of the oocyte.The 50 V series is our classic design with an internal diameter of 5 μm and a spiked tip beveled at 30°. They are supplied with an angle of 20° or 30°, with a tip to elbow length of 550 μm.Individually packaged. Box of 10 units.Sterilization performed by irradiation.

ICSI pipettes

Holding pipettes

Blastomere biopsy pipettes

022166 ICSIpipette–20°angle 10022167 ICSIpipette–30°angle 10

022168 Holdingpipette–outerdiameter100μm–20°angle 10022169 Holdingpipette–outerdiameter100μm–30°angle 10022170 Holdingpipette–outerdiameter120μm–20°angle 10022171 Holdingpipette–outerdiameter120μm–30°angle 10


DESCRIPTION / USEBlastomere biopsy pipettes are designed to remove blastomeres from embryos for preimplantation genetic diagnosis (PGD).

TECHNICAL SPECIFICATIONSThese pipettes are supplied with a tip angle of 30° and a tip to elbow length of 550 μm.Individually packaged. Box of 10 units.Sterilization performed by irradiation.

DESCRIPTION / USEHolding pipettes are used to hold an oocyte or an embryo in position during ICSI or any other micromanipulation procedure.

TECHNICAL SPECIFICATIONSThe classic series has an internal diameter of 20 μm and an outer diameter of 100 μm or 120 μm. All our holding pipettes are supplied with a tip angle of 20° or 30° and a tip to elbow length of 650 μm.Individually packaged. Box of 10 units.Sterilization performed by irradiation.

022172 Pipetteforblastomerebiopsy–30°angle 10

CE marked

8

HSVTM High Security Vitrification straw

DESCRIPTION / USE

Vitrification of oocytes and embryosThe kit is comprised of 3 parts:• a transparent High Security Vitrification ionomer-

ic resin straw;• a capillary tube with a transparent gutter and

co-injected colored handling rod; and• a blue insertion device.

Sterilization performed by irradiation.Individually packaged. Blister pack of 4 kits.

IDENTIFICATION

A straw identification system is available upon re-quest, comprising liquid nitrogen resistant adhesive labels and ink.

INSTRUCTIONS FOR USE

FILLING AND SEALING• Prepare the liquid nitrogen resistant identifica-

tion label for the HSV™ straw (maximum length: 40 mm) and apply it approximately 30 mm from the flared end of the straw; When positioned this way, the label does not cover the entirety of the colored rod or the biological sample zone.

• Connect the end of the blue insertion device to the end of the colored rod with its attached capillary. To do this, make sure you hold the rod very close to its opening with the fingertips of one hand, and the insertion device very close to its tip in the other.

• Prepare the sample for vitrification according to laboratory protocol and your medium supplier’s instructions;

• Using a micro-pipette and a binocular micro-scope, carefully deposit the sample into the gut-ter at least one millimeter from the end. The drop of sample must be as small as possible and never more than 0.5 μL. This is to avoid any contact with the outer straw. The HSV™ straw is designed to hold a single sample.

• Immediately insert the colored handling rod and capillary into the flared end of the HSV™ straw. Push until the rectangular portion of the blue in-sertion device comes into contact with the flared end of the straw.

• Carefully pinch the straw between your thumb and index finger around the colored rod and dis-connect the insertion device.

• Seal the open end of the straw using an SYMS®

seal.

Note: The time between vitrification and liquid ni-trogen immersion must not exceed the limit indi-cated by your medium supplier.

CE markedFDA 510(k) premarket notification clearance for the USA# K092398 for 4-8 cell and blastocyst stage embryos

Blisterpackof4HSVTMHighSecurityVitrificationstraws

025246 red 025248 yellow 025250 blue

025247 white 025249 pink 025251 green

Comingsoon HSVTMVitrificationstrawopeningdevice

VITRIFICATION


Coming soonOpening device for vitrification straws

CRYOPRESERVATION• Use tongs to hold the straw’s handling rod.

Immediately plunge the entire straw into liq-uid nitrogen, holding it vertically. While keep-ing it submerged, gently stir the straw in liquid nitrogen for a few seconds so as to avoid for-mation of an isolating air bubble layer around the straw.

STORAGE IN LIQUID NITROGENCryo Bio System has a range of long-term pack-aging and storage accessories for managing and organizing storage of HSVTM straws in liquid nitrogen (visotubes, goblets, canisters).

THAWING Sample thawing must be done under laminar flow.

• Prepare the thawing and dilution media according to laboratory protocol and your medium supplier’s instructions.

• Identify the HSV™ straw.• Use tongs to transfer the straw from the stor-

age container to the liquid nitrogen-filled transport container.

• Use tongs to lift the straw until the colored rod is exposed. Ensure that the end of the straw which holds the sample remains submerged in the liquid nitrogen.

• Hold the straw between two or three fingers and section it around the colored rod (away from the labelled area) using an opening de-vice. Once you have removed the sectioned part of the straw, hold the colored handling rod and slide the rod and capillary out.

• Submerge the end of the gutter into the first drop of thawing solution (use the volumes and temperatures recommended by your medium supplier). Move it gently to ensure the sample is properly transferred into the solution.


10

CRYOPRESERVATION

CBS™ High Security Tube, a Class IIa Medical Device

DESCRIPTION / USECryo Bio System has always been committed to developing products which contribute to optimal sample quality and security during cryopreservation. CBS™ High Security straws use cryo-resistant resin which can be entirely sealed before being put in cryogenic storage. Thanks to the new CBS™ High Security Tube, this concept has been extended to cryogenic ampoules and tubes.

The new CBS™ High Security Tube eliminates problems associated with cross-contamination and samples becoming degraded through oxidation and evaporation. Once it has been sealed, the CBS™ High Security Tube can be placed in storage containers and existing storage systems. High Security resin is com-patible with cryogenic storage temperatures, including during submersion in liquid nitrogen.

SECURITY• The sealing eliminates the risk of sample

cross-contamination and any risks related to the sample’s environment.

• Approved as hermetic and shatterproof at cryogenic temperatures, including liquid nitrogen temperatures

• Indestructible when used as advised.

TRACEABILITY OPTIONS• Color inserts for easy identification in a cryo-

genic environment• Cryo-resistant RFID chips embedded into

inserts• Inserts identified using 2D data matrix code• Compatible with cryo-resistant labels

COMPATIBILITY• Standard racks, containers (9x9 or 10x10),

canes• Manual and automatic filling systems• Programmable freezers• Cryogenic storage conditions for use with o Liquid nitrogen o Vapor phase liquid nitrogen o Mechanical freezers

HIGH QUALITY CRYOPRESERVATIONCBS™ High Security Tubes’ expert material and manufacture have benefitted from the design concept behind CBS™ High Security straws. The thermal sealing of CBS™ contain-ers allows for immediate, complete sealing in liquid nitrogen. Also available is a wide range of identification solutions which avoid any of the risks to sample quality associated with tem-perature changes.

APPLICATIONS• Assisted reproduction• Sample banks for pharmaceutical, medical

and scientific research and biodiversity• Blood banks and blood donation centers• Cellular and gene therapy units• Packaging heat-sensitive vaccines and

medication• Sperm and ovarian tissue banks

022252 Bagof20sterileCBS™HighSecurityTubes023722 Bagof50sterileCBS™HighSecurityTubes022251 Bagof100non-sterileCBS™HighSecurityTubes

024108 Bagof500whiteinsertsforCBS™HighSecurityTubes024109 blue024110 green 024111 yellow024112 red024113 pink

024114 Bagof100whiteinsertswith2DdatamatrixcodeforCBS™HighSecurityTubes

023836 AutoclavablescissorsforCBS™HighSecurityTubes

TECHNICAL SPECIFICATIONSUsable working volume: 1.2 mL Dimensions: 48 mm, ø 11.8 mm Weight: 1.8 g Material: cryogenic grade ionomeric resin Usage temperature range: from + 40°C down to cryogenic temperature -196°C Sterility: batch certified sterile SAL 10-6 radiation sterilized DNase and RNase free Endotoxin-free MEA tested per batch (on mouse embryos) MAT tested Liquid nitrogen: liquid and vapor phase vali-dated 2D data matrix code capacity: 10 numeric characters 2D data matrix code symbol size: 12 x 12 (rows x columns)

SHELF-LIFE OF STERILE PRODUCTS 022252 and 023722Do not use the device once the year, month or day indicated has passed.

COMPLIANCE WITH INTERNATIONAL STANDARDSCompliant with CE standards according to the European Medical Devices Directive 93/42/CE modified 2007/47/CE (Class IIa)

A UNIQUE FULLY SECURE SYSTEM FOR LIQUID NITROGEN STORAGE


CBS™ High Security Tube, a Class IIa Medical Device CBS™ High Security embryo and sperm straws with tripartite plug

DESCRIPTION / USECBS™ High Security embryo and sperm straws with woven plugs are specially designed for long-term cryopreservation of human biological samples (notably in liquid nitrogen). Manufactured from biocompatible materials, they are used as assisted reproductive technology for sperm and embryo cryopreservation in particular. Sterilization performed by irradiation.Individually packaged. Bag of 5 blister packs containing 4 units.

TECHNICAL SPECIFICATIONS Length: 133 mmInternal diameter: 2.5 mm

IDENTIFICATIONCBS™ High Security embryo and sperm straws have a fully secure upper identification compartment which houses a colored rod bearing identi-fication data.

FILLING AND SEALINGCBS™ High Security embryo and sperm straws are filled by aspiration using a transparent sterile filling nozzle and a micro-aspirator in accordance with the applicable protocol. They are guaranteed to be hermetically sealed only if the sealing is done using an SYMS or SYMS III on clean, dry inner and outer ends.

025292 Blisterpackof20CBS™HighSecurityembryoandspermstrawswithtripartiteplug

Bagof10weightedidentificationrods30mm

019021yellow 019022green 019023blue

019024orange 019025white 019026red

025292CBS™HighSecurityembryoandspermstrawwithtripartiteplug

024856Boxof100transparentsterilefillingnozzles

STORAGE IN LIQUID NITROGENCryo Bio System carries a line of long-term packaging accessories for stor-age and management of CBS™ High Security embryo and sperm straws in liquid nitrogen (visotubes, goblets, canisters).

THAWING INSTRUCTIONS FOR CBS™ HIGH SECURITY STRAWSOnce the required straw has been located, it can be identified thanks to the color of the identification rod.


CE markedFDA 510(k) premarket notification clearance for the USA # K002595

12

CBS™ High Security sperm straw with tripartite plug

DESCRIPTION / USEStorage and preservation of sperm.CBS™ High Security straws are designed to have special properties and filling and sealing methods to guarantee that human samples are never exposed during cryo-preservation, especially when using liquid nitrogen. Manufactured with biocompati-ble materials, the CBS™ High Security straw is designed for use in Assisted Reproduction Technologies, particularly for cryopreser-vation of sperm. The CBS™ straw is a clear, flexible tube made of ionomeric resin.Length: 133 mmInternal diameter: 2.5 mmSterilization performed by irradiation.

CBS™ 0.3 mL strawThis straw has two separate parts which are separated by a white safety plug. The first part holds semen to a volume of 0.3 mL.The second part allows for a colored identi-fication rod to be inserted and can be used for handling when the straw is placed in liquid nitrogen.

CBS™ 0.5 mL strawSame technical specifications as the 0.3 mL straw, except that the working volume is 0.5 mL, and the outer part of the cotton safety plug is colored.

IDENTIFICATION0.3 mL and 0.5 mL CBS™ straws canbe reliably identified via a barcode and/or alphanumerically using an outer jacket. This jacket can be removed after thawing and ensures that samples are traceable.

FILLING AND SEALINGStraws are to be filled individually using a transparent sterile filling nozzle and micro- aspirator or a syringe with a special adapter.Sealing is only guaranteed to be hermetic when the ends are clean, dry and sealed using an SYMS or SYMS II.When large numbers of CBS™ High Security straws are being used (at a sperm bank, for example), they can be filled and sealed using a semi-automatic PACE ma-chine (filling and sealing) or a MAPI ma-chine (filling, sealing and jacket printing).

STORAGE IN LIQUID NITROGENCryo Bio System carries a line of long-term packaging accessories for storage and management of CBS™ straws in liquid nitrogen.

THAWING INSTRUCTIONSFOR CBS™ HIGH SECURITY STRAWSAfter verifying the location of the desired straw, its identification is confirmed by the color of the internal identification tube (CBS™ 0.3 mL), its plug (CBS™ 0.5 mL) and/or its outer jacket.The straw is then removed from its storage visotube, thawed by being submerged for a few seconds in warm water (37°C max-imum) and wiped down. After drying, it is ready to use.


CRYOPRESERVATION


010287 CBS™HighSecurity0.3mLspermstrawwithtripartiteplug 20

010288 CBS™HighSecurity0.3mLspermstrawwithtripartiteplug 100

CBS™HighSecurity0.3mLspermstrawwithwhitejacketandcoloredrod 75

017797 red 017798 white 017799 yellow

017800 blue 017801 green 017802 purple

014651 CBS™HighSecurity0.5mLspermstrawwithwhitecottonplug 20

014650 CBS™HighSecurity0.5mLspermstrawwithwhitecottonplug 100

CBS™HighSecurity0.5mLspermstrawwithcoloredcottonplug 100

016584 red 016611 yellow 016612 green

016613 blue 016614 orange 016615 grey

024856 Boxof100steriletransparentfillingnozzles

Bagof10weightedidentificationrods30mm

019021 yellow 019022 green 019023 blue

019024 orange 019025 white 019026 red

Identificationjacket 50

010272 red 010273 white 010274 orange

010275 green 010276 blue 010277 yellow

014939 transparent

014175 Setof2indelibleblackmarkers 4

016730 Sterileconnectiontubingforsyringe(individuallypackaged) 1

016731 Sterileconnectiontubingforsyringe(packof5units) 5

010284 SterileemptyingdeviceforCBS™HighSecurityspermstraws 20

CE markedFDA 510(k) premarket notification clearance for the USA# K002595

14

DESCRIPTION / USEThe CBSTM filling nozzle is for filling CBSTM High Security embryo and sperm straws. It guarantees that your straws are hermetically sealed by preventing the sealing zone from having any contact with liquid when the sample enters the straw.The CBSTM filling nozzle is single use and individ-ually packaged in peelable blister packs. They come in boxes of 100. The CBSTM filling nozzle has an overmoulded body covering a transparent capillary. It is specifically designed for hermetic connections with the end of CBSTM High Security embryo and sperm straws.


Sterile filling nozzles for CBS™ High Security straws

024856 Boxof100sterilefillingnozzlesforCBS™HighSecuritystraws

CBS™ High Security tissue 2 mL straw

INSTRUCTIONS FOR USE• Prepare the identification label and stick it onto

the outer side of the CBS™ High Security tissue straws.

• Use the SYMS sealing system with a guide for 2 mL straws to seal the ends of the straw, or use the SYMS II.

• Carefully deposit the piece of tissue inside the straw using tweezers.

• Seal the other end of the straw using the SYMS or SYMS II – ensure the straw does not come loose from the sealer’s jaws.

DESCRIPTION / USECryopreservation of tissue fragments.Sterilization performed by irradiation.Individually packaged. Bag of 5 blister packs containing 4 units.

IDENTIFICATIONCBS™ High Security 2 mL tissue strawscan be identified using labels which use liquid nitrogen resistant ink and adhesive.

STORAGE IN LIQUID NITROGENCryo Bio System carries a line of long-term packaging accessories for storage and man-agement of CBS™ straws in liquid nitrogen.


018960 Blisterpackof20CBS™HighSecurity2mLtissuestraws

CRYOPRESERVATION


PETG sperm straw

PETG embryo straw

Sterilization performed by irradiation.Bag of 100 units.

IDENTIFICATIONStraws can be printed onto using an ink jet printer, or identified via liquid nitrogen resis-tant labels or indelible marker pen.

FILLING AND SEALINGFor storage of sperm in preparation of intra-uterine insemination or in vitro fertilization, the PETG straws are filled with a micro- aspirator (or with a syringe and an adapter) and closed with short ID plugs of 13 mm in length.

SHELF-LIFEThree years from date of manufacture.

DESCRIPTION / USEFreezing and storing human spermPETG sperm straws are clear flexible straws made of a copolymer.• 0.25 mL strawLength: 133 mmInternal diameter: 1.6 mm• 0.5 mL strawLength: 133 mmInternal diameter: 2.5 mm

They are composed of two distinct com-partments separated by a sliding safety plug.• The section which holds semen has a vol-

ume of 0.21 mL or 0.47 mL.• The other section is used as an aspiration

zone during filling.

006433 5blisterpacksof20rigidembryostraws91mm006578 5blisterpacksof20rigidembryostraws133mm014102 5blisterpacksof20flexible91mmembryostraws014103 5blisterpacksof20flexible133mmembryostraws

Blisterpackof20sealingandidentificationrods(42mm)007437 yellow 007438 purple 007440 red007442 orange 014484 pink 014485 blue014486 grey 014487 green 014488 royalblue014489 white

007342 Yellowsterile(13mm)sealingandidentificationrods 20

DESCRIPTION / USEFreezing and storage of human embryos.PETG embryo straws are flexible or rigid clear tubes made of copolymer. The PETG embryo straws are closed at one end by a double-woven cotton plug, enclosing a dose of powder that polymerizes when in contact with liquid.

Their useful volume is:• 0.15 mL for 91 mm long straws • 0.25 mL for 133 mm long straws.The internal diameter is 1.6 mm for both models.

Sterilization performed by irradiation.Blisters of 4 x 5 units.

IDENTIFICATIONStraws are identified using specific colored rods.

FILLING AND SEALINGFor embryo cryopreservation, the straws are filled with the use of a micro-aspirator and are sealed with a 42 mm long plug for 91 mm straws, and a 13 mm long plug for 133 mm straws.


010261 TransparentPETG0.25mLspermstraw100014550 TransparentPETG0.5mLspermstraw100007342 Yellowsterile(13mm)sealingandidentificationrods 20

CE marked

CE marked

16

BMP 21 and BMP 51 printers

Micro-aspiratorDESCRIPTION / USEManual straw filling.The micro-aspirator is used to manually fill all straws manufactured by Cryo Bio System without any risk of contamination.

INSTRUCTIONS FOR USE• Hold the micro-aspirator horizontally.• Carefully connect the straw by inserting it into the

conical nozzle of the tubing. The tubing protruding

from the micro-aspirator head should be around 10 cm long.

• Aspirate the biological fluid by slowly turning the aspirator’s thumb wheel in the opposite direction to the adaptor until the desired filling height has been reached.

• Remove the straw from the adaptor.

No special maintenance is required.

ACCESSORIES

DESCRIPTION / USEFor identifying CBS™ High Security straws and HSVTM High Security Vitrification straws Brady labels have been specially designed to resist the extreme environments found in laboratories. Warranty: 12 months.

WARNINGIt is strongly recommended to stick the label so it over-laps the straw itself if it is to be stored in liquid nitrogen.

TECHNICAL SPECIFICATIONSPrinting technology: thermal transfer (long-lasting, smear-free)Prints multiple lines of text.

024498 BMP 21 printer 1

024500 220VadapterforBMP21printer 1

024499 Case for BMP 21 printer 1

024501 Polyester9.5mmribbonforBMPprinter21 1

BMP 21 PRINTERL x W x H: 228.6 x 114.3 x 63.5 mm Weight: 0.750 kg

BMP 51 PRINTERL x W x H: 262 x 91 x 152 mmWeight: 1.2 kg

Operating temperature: 4° - 49°CFCC Class A approved (printer) UL and CSA certified (cartridges)

025445 BMP51printer+LabelMark5software 1

025446 BMP 51 printer 1

025450 LargelaminatedlabelforCBSTM Tubes 1

025451 SmalllaminatedlabelforCBSTMTubes 1

026433 Polyester9,5mmribbonforBMP51printer 1

026434 Polyester12,7mmribbonforBMP51printer 1

BMP 21 PRINTER

BMP 51 PRINTER

014498 Micro-aspirator(comeswith2nozzlesandtubing)

014500 Nozzleandtubingformicro-aspirator


Canisters

DESCRIPTIONCanisters are cylindrical sleeves for storing stacks of goblets in liquid or vapor phase nitrogen. Storage in goblets or canisters is convenient for all types of nitrogen containers or electric freezers.

There are five heights of canisters which can con-tain between 2 and 6 goblets. A colored flag at the end of the lifter (a curved metal stem which lifts up the column of goblets) lets users identify each canister by sight.Canisters are placed in the nitrogen storage con-tainer, which is then filled with liquid nitrogen.

The lifter helps users pile up and lift out goblets one by one. Goblets are only handledin the cold environment of the neck of the nitro-gen storage container, so that the samples’ tem-perature remains stable.

Diameter: 70 mm.

Canisterwithlifterandwhiteflagforclassicø65mmgobletswithoutacover 10

007147 2shelves 007148 3shelves 007149 4shelves

007150 5shelves 007151 6shelves

CanisterwithlifterandwhiteflagforDaisygoblets 1

008644 2shelves 008645 3shelves 008646 4shelves

007198 5shelves 007199 6shelves

Coloredplasticflag 20

007328 white 015693 yellow 015694 salmon

015695 pink 015696 darkgreen 015697 turquoise

015698 orange 015699 purple 015706 grey

014175 Setof2indelibleblackmarkers 4

005524 Shortstainlesssteeltongs(250mm) 1

005523 Longstainlesssteeltongs(700mm) 1

18

ACCESSORIES

CBS™ visotubes and goblets

DESCRIPTION / USEFreezing and storage of biological materials in straws. Arrangement using color coding of straws prior to storage in liquid nitrogen. CBS™ High Security straws are placed in visotubes, then into colored goblets for storage in cryopreservation freezers. Goblets come in 8 different colors and their sub-compartments (visotubes) in 12 different colors.

In the Daisy configuration, goblets hold 12 visotubes of different colors and up to 168 straws of 0.3 ml or 0.5 mL.

Diameter: 65 mm (67 mm with cover)Goblet height: 117 mmTotal height of the Daisy goblet (withcover): 135 mmHeight of triangular visotubes: 120 mmHeight of round visotubes with 17.5 mm diameter: 133 mm

Triangularvisotube

Roundvisotube

ø 17.5 mm

Goblet (only)ø 65 mm

with cover

Daisy gobletCoverfor ø 65 mm goblet

(height: 40 mm) (height: 70 mm)

COLORS

CAPACITY

Transparent 006924 - 021224 021366 006321 015144 015152 Black - 018153 - - - - - Brown - 018147 - - - - - Red - 018146 021228 - - 015146 015154 Green - 018145 021226 - - 015147 015155 Blue - 018148 021225 - - 015151 015159 Grey - 018144 - - - 018141 018142 Purple - 018152 021229 - - 015150 015158 Yellow - 018143 021227 - - 015148 015156 Pink - 018149 - - - - - Orange - 018150 - - - - - Pistachio - 018151 - - - 015149 015157

PACKAGING 100 100 10 50 10 1 5

PETG0.25mLstraw 55 45 820 - - 540 540

PETG0.5mLstraw 20 20 365 - - 240 240

HSVTM CBSTM

HighSecuritystraw 20 20 365 - - 240 240

HighSecurityCBS™straw 14 14 225 - - 168 168

133

mm

135

mm

120

mm

70 m

m40 m

m


COLORS

CAPACITY

CBS™ visotubes and goblets

DESCRIPTION / USEFreezing and storage of biological materials in straws. Arrangement using color coding of straws prior to storage in liquid nitrogen.

• Standard goblet ø 35 mm Diameter: 35 mm Height: 122 mm

• Standard goblet ø 65 mm Diameter: 65 mm Height: 117 mm

• Polygonal, hexagonal and round visotubes Height: 120 mm

All visotubes are also available in bags of 300 units (in identical colors and diameters).Please contact us for further information.

Polygonalvisotube

Hexagonalvisotube

Roundvisotubeø 7.1 mm

Roundvisotubeø 9.2 mm

Roundvisotubeø 10 mm



Gobletø 35 mm

Gobletø 65 mm

White 020470 020486 020492 020499 020505 020511 020517 020523 024392 Blue 020472 020487 020493 020500 020506 020512 020518 - 006755 Green 020482 020488 020494 020501 020507 020513 020519 - 006754 Yellow 020483 020489 020495 020502 020508 020514 020520 - 006756 Orange 020484 020490 020496 020503 020509 020515 020521 - - Red 020485 020491 020497 020504 020510 020516 020522 - 006397 Purple - 022141 - - - - - - 006757 Pistachio - - - - - - - - 006753

PACKAGING 10 10 10 10 10 10 10 10 10

PETGstraw0.25mL 30 90 7 10 14 22 27 200 820

PETGstraw0.5mL 15 36 2 5 5 8 10 94 365

HSVTM CBSTM HighSecuritystraw 14 36 3 6 6 10 12 - -

CBS™HighSecuritystraw 8 22 1 3 4 7 7 75 225

Inagobletø35mm 4 1 15 10 8 5 5 - -

Inagobletø65mm 15 7 60 37 31 21 19 - -

20

EQUIPMENT

SYMS III

The SYMS III thermally seals 0.3 mL, 0.5 mL and 2 mL CBS™ High Security straws as well as HSVTM High Security Vitrification straws and CBS™ High Security Tubes for top quality cryopreservation of biological samples.

CE marked

KEY CHARACTERISTICS• A multilingual interface (French, English,

Spanish and Chinese) + 4 optional languages

• A color touch screen that can be used while wearing laboratory gloves

• View of the machine cycles: preheating, ready status, sealing in progress, etc.

• Specially adapted pre-programming for each CBS™ product’s sealing tempera-ture and time

• Two sealing methods: fully automatic detection or operator-controlled via the touch screen

• Alarms and alert messages signal faults• Tracking display and maintenance

modes display• Easy to clean and maintain

INSTRUCTIONS FOR USE• Position the CBSTM High Security straw or

CBSTM High Security Tube as required.• Position the CBS™ High Security straw

or the CBS™ High Security Tube on the holder and gently slide it into place.

• Automatic: The machine automatically detects the product to seal.

• Manual: Commands are entered on the touch screen.

TECHNICAL SPECIFICATIONSL x W x H: 335 x 250 x 105 mmWeight: 3 kgPower Supply: 110 or 220 V / 60 or 50 Hz

SEALER FOR CBS™ HIGH SECURITY STRAWS, TUBES AND VITRIFICATION STRAWS

025547 SYMSIIIsealer

026550 SealingjawssetforSYMSIII


SYMS

CE marked

DESCRIPTION / USECryo Bio System has developed a sealer for CBS™ High Security ionomeric resin straws.The sealing device is electronically oper-ated by a push-button or foot pedal as desired.

INSTRUCTIONS FOR USE• Once the straw has been filled, remove its

filling nozzle.• Immediately seal the end which held

the nozzle. Once this has been done, there is no risk of the sample becoming contaminated.

• Insert a colored identification rod or, if necessary, use a colored jacket.

• Seal the other end of the straw.

• Either freeze or vitrify, depending on the best method for the product you are cryopreserving.

TECHNICAL SPECIFICATIONSL x W x H: 230 x 220 x 170 mmWeight: 6 kgPower Supply: 220 V / 60 or 50 Hz

016399 CompleteSYMSsealerforCBS™HighSecuritystraws

009848 SparepartsforSYMSsealer(Teflonstrips,fuses,etc.)

MANUAL SEALER FOR CBS™ HIGH SECURITY STRAWS AND VITRIFICATION STRAWS

22

PACE

DESCRIPTION / USE

A semi-automatic system for packaging biological samples in CBS™ High Security straws.

From an open primary sample tube, PACE auto-matically fills and thermally seals both ends of CBS™ High Security 0.3 or 0.5 mL straws - which can be pre-identified.PACE is the ideal CBS™ machine for managing medium- or large-scale collection protocols. PACE is compatible with CBS™ High Security straws that are pre-identified with barcodes and/or alphanumerical codes, and those that are not yet identified.The system includes a filling and sealing unit and a control box.

GENERAL CHARACTERISTICS

PACEmachinecyclestages:• Straws are placed in the hopper,• The primary sample tube and injection tubing

are put in place manually,• The straws are filled using aspiration via the

built-in pumps,• The straws are thermally sealed at both ends,• These straws are manually transferred to their

storage destination (visotubes and goblets).

The throughput of straws is fully dependent on the protocol (e.g. number of straws filled per pri-mary tube and the number of different biological products).

Start-up time, maintenance time and downtime: 15 minutes per day.

Potentially contaminated parts can be cleaned using an alcohol solution or a non-corrosive surface decontaminant.

The PACE system can be placed on the laboratory bench or under laminar flow with sterile straws and filling tubing. It only requires a power supply.

TECHNICAL SPECIFICATIONS• PACEL x W x H: 380 x 300 x 370 mmWeight: 25 kg

• Control boxL x W x H: 410 x 365 x 155 mmWeight: 14 kg

Power Supply:110 or 220 V / 60 or 50 Hz

SEMI-AUTOMATIC FILLING AND SEALING SYSTEM FOR CBS™ HIGH SECURITY STRAWS

PACE, semi-automatic filling and sealing system for CBS™ High Security straws

018451 PACE220V/50Hz

018450 PACE110V/60Hz

007454 SterileCBS™injectiontubing(5 x 10) 50

018620 SterilelongblueCBS™injectiontubing(5 x 5) 25

007451 SterileCBS™aspirationtubing(5 x 10) 50

CE marked

EQUIPMENT


007454 SterileCBS™injectiontubing(5x10) 50

018620 SterilelongblueCBS™injectiontubing(5x5) 25

007451 SterileCBS™aspirationtubing(5x10) 50

MAPI

DESCRIPTION / USE

An automatic system for packaging and identification of biological samples in CBS™ High Security straws.

From an open primary sample tube, MAPI automatically fills CBS™ 0.3 or 0.5 mL straws, seals them, prints identification and validates their ID in-line by reading barcodes or alpha-numeric data.The MAPI system consists of the filling robot and its control box, a special liquid nitrogen compatible ink-jet type printer and a PC with the CBS™ pilot software. The machine is in-stalled on a support table specially designed for top ergonomic quality.

GENERAL CHARACTERISTICS

MAPImachinecyclestages:• Straws are placed in the hopper,• The barcode is read or the primary sample

tube code is entered manually,• The primary sample tube and injection tub-

ing are put in place manually,• The straws are filled using controlled aspira-

tion via the built-in pump,• The straws are thermally sealed at both

ends,• Ink-jet printing of barcode and text of up to

10 characters with a check digit (code 128),• Straws are validated via their barcode,• The approved straws are selected,• The straws are manually transferred to their

storage destination (visotubes and goblets).

The throughput of straws is fully dependent on the protocol (e.g., number of primary tubes and number of straws filled per primary tube).Setup, maintenance and shutdown: 15 min-utes per day.All potentially contaminated parts can be cleaned with ethanol or non-corrosive de-contamination fluids.The ink-jet printing system requires active evacuation of its solvent vapors.

MAPIsoftware

MAPI software is installed on the computer provided with MAPI machines, so that users can controlthe cycle: distribution, filling, sealing, printing and re-reading of straws.

The code to be printed on the straw can be either a barcode read from the primary tube, keyboard entered or programmed fixed text.

TECHNICAL SPECIFICATIONS • MAPIL x W x H: 760 x 260 x 470 mmWeight: 35 kg

• Support tableL x W x H: 1200 x 600 x 900 mmHeight with machine: 1260 mmWeight with machine: 102 kg

• Control boxL x W x H: 430 x 380 x 190 mmWeight: 14 kg

• Domino printerL x W x H: 650 x 450 x 375 mmWeight: 20 kg

Fume extraction system for the printer is not included (cable diameter: 80 mm)

Power Supply:110 or 220 V / 60 or 50 Hz

AUTOMATIC FILLING, SEALING AND IDENTIFICATION SYSTEM FOR CBS™ HIGH SECURITY STRAWS

007218 MAPI,automaticfilling,sealingandidentificationsystem forCBS™HighSecuritystraws

CE marked

24

CBS™ NANO-DIGITCOOL Controlled rate freezerTHE BEST COMPACT FREEZER FOR DAILY USE IN ASSISTED REPRODUCTIVE TECHNOLOGY CENTERS

DESCRIPTION / USE

Digitcool freezers are used to cryopreserve all types of biological products (plasma, blood cells, tissues and gametes for example). Digitcool stainless steel freezers are fully programmable and easy to disinfect.

KEY CHARACTERISTICS

• Ideal traceability thanks to CryoBioSoft soft-ware which is compliant with standard CFR 21 Part 11

• Atomized liquid nitrogen flows vertically to en-sure that the temperature of the container is fully stable throughout

• Functions in emergency mode: the freezing cycle continues even if the IT system fails

• Straws and tubes can be frozen with automatic or manual seeding

• Remote view of freezing stages using colored light signals

TECHNICAL CHARACTERISTICS

• L x W x H: 722 x 475 x 472 mm• Weight: 55 kg• Inner dimensions of the freezing chamber:

238 x 178 x 200 mm• Volume: 11.5 L• Capacity: o 192 tubes of 2 mL o 33 CBS™ High Security straws o 15 DF 200 bags• Power: 1200 W• Voltage / Frequency: 220 V / 50 Hz / 8 A• Freezing speed: -0.1 to -60°C/min• Usage temperature range: + 50°C to - 150°C • Software: CryoBioSoft

025327

DF 200 bags rack for Nano-Digitcool

025433 Tubes rack for Nano-Digitcool

024572

Straws rack for Nano-Digitcool

024804:Nano-Digitcool

025680:Nano-DigitcoolMedicalDevice

EQUIPMENT


SYSO

DESCRIPTION / USE

The SYSO opening machine is a worktop device for lab benches which can be used under laminar flow. It uses a hot wire to open CBS™ High Security Tubes and has applications which include:• Epidemiological research departments• Biobanks• Serum banks• Cellular and gene therapy units• Vaccine-manufacturing pharmaceutical compa-

nies (living cells)• Gene banks (animal, plant, microbiological)• Reproductive biology laboratories• Gamete and gonadal tissue banks

KEY CHARACTERISTICS

• The carrousel can hold up to 6 CBS™ High Security Tubes, which can either be opened individually or in succession.

• Easy, intuitive touch screen• 8 languages• The wire is systematically sterilized by pyrolysis

after each tube is opened.

INSTRUCTIONS FOR USE

• UNITARY CUT: Place the tube on the carousel (the position of the tube’s sealing lugs must be as indicated on the carousel). Make sure the tube sits snugly in its holder. Press UNIT CUT. Once the straw has been opened, remove the tube from the carousel.

• MULTI-CUT Place 6 tubes on the carousel (the position of the tubes’ sealing lugs must be as indicated on the carousel). Make sure the tubes sit snugly in their holders. Press MULTI-CUT. Once the tubes have been opened, remove them from the carousel.

TECHNICAL SPECIFICATIONS

L x W x H: 358 x 275 x 157 mmWeight: 2.5 kgPower Supply: 110 or 220 V / 60 or 50 Hz

024922 AutomaticSYSOmachine foropeningCBS™High SecurityTubes

NEW AUTOMATIC EQUIPMENT FOR OPENING CBS™ HIGH SECURITY TUBES

26

Automatic sperm collector NEW!

• Simulate the vaginal spasms• Visual and auditory stimulation• Adaptable to patient anatomy• No cross contamination• Smooth and efficient

Semen collection process comfortable, fast and safe

Offer your patients the best chances to give life!

Artificialvaginasmallmodel 026368 x1

Artificialvaginamediummodel 026369 x1

Artificialvaginalargemodel 026370 x1

3Dviewerglasseswithstoragespace026373 x 1

Sterile single use condomnon-spermicidal026371 x 20

Singleusediaphragm 026372 x20

CompleteSpermCollectorportablewith 1 adaptable table, 3 artificial vaginas (1 little, 1 medium and 1 large), 20 single use diaphragms and 3D viewer glasses026365

CompleteSpermCollectorV4with 3 artificial vaginas (1 little, 1 medium and 1 large) and 20 single use diaphragms026367

EQUIPMENT


Promotional code

28

Cryo Bio System FranceGroupe IMV TechnologiesZI n°1 Est61300 L’Aigle, France+33 (0)[email protected]

Cryo Bio System NetherlandsAmperestraat 48912 AD Leeuwarden, The Netherlands+31 58 [email protected]

Cryo Bio System ItalyVia Stefano Merli29122 Piacenza, Italy+39 0523 36 00 [email protected]

Cryo Bio System USA11725 95th Avenue NorthMaple Grove, MN 55369, USA+1 763 488 [email protected]

Cryo Bio System IndiaPlot No 750, Phase V, Udyog ViharGurgaon 122016 (Haryana), India+91 124 400 44 30/31/[email protected]

Cryo Bio System ChinaFloor 12, Building A, No. 400 Pudian Road, Pudong, Shanghai 200122, China+86 21 50586948,[email protected]

LejaLuzernestraat 102153 GN Nieuw-VennepThe Netherlands+31 (0)252-621,[email protected]

w w w . c r y o b i o s y s t e m - i m v . c o m [email protected] Ed

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Documents

Assisted Reproductive Technologies...Assisted Reproductive Technologies LIFE. Cryo Bio System is exclusively dedicated to human life sciences. It provides innovative solutions for