ASCO Advanced Course

Ahmed Farouk Salem1,3, Niccola Funel1,2, Luca Emanuele Pollina1, Ugo Boggi2, Marco Del Chiaro2, Mosca Franco2, Generoso Bevilaqua1,

Daniela Campani1

1Division of Surgical, Molecular and Ultrastructural Pathology, 2Division of General Surgery and Transplantation. Department of Oncology, Transplants and

Advanced Technologies in Medicine, University Hospital of Pisa. Pisa, Italy.3Molecular Drug Evaluation Department, National Organization for Drug Control

and Research. Cairo, Egypt.

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

ASCO Advanced Course

20% of Pancreatic cancer patients are candidates for surgical resection.

Chemo- and radiotherapyrole remains controversialrole remains controversial.

Pancreatic cancer stillPancreatic cancer still correlates with very poor prognosis .

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

ASCO Advanced Course

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

ASCO Advanced Course

I P C-In Pancreas Cancer….

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

ASCO Advanced Course

CellCellMembrane

ds siRNA ERK1/221-23 nt

mRNAERK1/2

Electroporation Pulse

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

p

ASCO Advanced Course

20nM 80nMsiRNAsiRNA

2X

40nM

siRNAWOEP

PPCCCPP117

EP

80 M

40nMsiRNA2X

EPPP117

80nMsiRNA2X

CTRL

PPCCC: Primary Pancreatic Cancer Cell Culture EP: Electroporation CTRL: Control

siRNA ERK 1/2

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

PPCCC: Primary Pancreatic Cancer Cell Culture EP: Electroporation CTRL: Control

ASCO Advanced Course

80 nM siRNA ERK 1/2 Electroporation Only

AB against ERK1/2 AB against ATPasesiRNA ERK 1/2

with ElectroporationElectroporation Only

with Electroporation

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

ASCO Advanced Course

ERK 1/2 increases proliferation, cell survival, and resists apoptosis induced by chemotherapeutic agents.

siRNA appears to be powerful, specific inhibitor of oncogenes products.

Electroporation is cost-effective, reproducible, and rapid technique to deliver macromolecules inside cell cultures.

Examine siRNA ERK1/2 efficacy on other pancreatic cell cultures.

Determine chemo-sensitivity of pancreatic cell cultures transfected with siRNA ERK1/2siRNA ERK1/2.

Develop in-vivo electroporation technique.

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt

ASCO Advanced Course

GRAZIE PER LA VOSTRAATTENZIONE

South & East MediterraneanCollege of Oncology

26 – 28 March 2008Cairo - Egypt