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Title: Suppression of the MADS-box gene SlMBP8 accelerates fruit ripening of tomato (Solanum lycopersicum)
Running Title. Silencing of SlMBP8 accelerates fruit ripening in tomato
Authors: Wencheng Yin, Zongli Hu, Baolu Cui, Xuhu Guo, Jingtao Hu, Zhiguo Zhu, Guoping Chen *
Laboratory of molecular biology of tomato, Bioengineering College, Chongqing University, Chongqing 400044,
People’s Republic of China
* Guoping Chen (Author for Correspondence): Room 523, Bioengineering College, Chongqing University,
Campus B, 174 Shapingba Main Street, Chongqing 400030, P.R. China; Tel: 00862365112674; Fax:
00862365112674; E-mail: [email protected]
Supplementary Figure S1.
Multiple sequence alignment of SlMBP8 and other MADS-Box proteins. Identical amino acids are shaded in black,
and similar amino acids are shaded in gray. Accession numbers for the proteins listed are as follows: SlMADS-RIN
(NM_001247741.1), SlMADS1 (NP_001234380), SlFYFL (KF709444), SlTDR4 (FUL1) (NM_001247244),
SlMBP7 (FUL2) (NP_001294867).
Supplementary Figure S2.
Hairpin construct of the SlMBP8gene for double-stranded RNAi vector. The SlMBP8 gene-
specific sequence in the antisense and sense orientations was linked with a PDK gene fragment
and as a transcriptional unit for hairpin RNA expression which controlled by the CaMV 35S
promoter and terminated by the 35S terminator. Among which, SpeI and XbaI are isocaudamers.
Supplementary Figure S3.
MADS-box gene SlMBP15 expression in SlMADS1-silenced and wild-type fruits.
Supplementary Figure S4.
Seedling of wild-type treated with 0, 1.0, 2.0, 5.0, 10.0 and 20.0 μM ACC.
Supplementary Table S1. Primers used for quantitative real-time PCR analysis
Gene Primer sequence: (5ˊ-3ˊ) GeneBank ID
SlCAC-Q-F
SlCAC-Q-R
CCTCCGTTGTGATGTAACTGG
ATTGGTGGAAAGTAACATCATCG
XM-004244491
SlMBP8-Q-F
SlMBP8-Q-R
TCCAGGTCGCAGAGGTGTCG
TGTTGTCCACAAATCATG
XM_004252664
SlMBP15-Q-F
SlMBP15-Q-F
GGCAGAGCACTCTAAAGACTC
GGAAATTCAGGGTTATTCTC
XM_004252663
ACO1-Q-F
ACO1-Q-R
ACAAACAGACGGGACACGAA
CTCTTTGGCTTGAAACTTGA
NM_001247095.1
ACO3-Q-F
ACO3-Q-R
CAAGCAAGTTTATCCGAAAT
CATTAGCTTCCATAGCCTTC
Z54199
ACS2-Q-F
ACS2-Q-R
GAAAGAGTTGTTATGGCTGGTG
GCTGGGTAGTATGGTGAAGGT
AY3269
E4-Q-F
E4-Q-R
AGGGTAACAACAGCAGTAGCA
CCCAACCTCCGTCTTCAC
S44898.1
E8-Q-F GGCACCATTCAACATACCG X13437.1
E8-Q-R CTTTCACCGAAGAAGCACG
ERF1-Q-F
ERF1-Q-R
TTTTAGTATCGGATGGACG
GGCGGAGAAACAGAAGTA
NM_001247919.1
RIN-Q-F
RIN-Q-R
GGAACCCAAACTTCATCAGA
TTGTCCCAAATCCTCACCTA
NM_001247741.1
PSY1-Q-F
PSY1-Q-R
AGAGGTGGTGGAAAGCAA
TCTCGGGAGTCATTAGCAT
EF157835
LoxA-Q-F
LoxA-Q-R
GAGGCGTGGGATAGGA
GGATACGGGTAGTCAGCA
U09026.1
LoxB-Q-F
LoxB-Q-R
TGCTACAATGACTTGGGTGAA
CCTGTCCTGCCTCTACG
U09025.1
PDS-Q-F
PDS-Q-R
GCTTTACCCGCTCCTTTA
ACCTTGCTTTCTCATCCA
S36691.1
ZDS-Q-F
ZDS-Q-R
GGTGGGTGCTGAAAAAAAT
GGAAAGCGGAAATCAAGTT
NM_001247454.1
PG-Q-F
PG-Q-R
ATACAACAGTTTTCAGCAGTTCAAGT
GGTTTTCCACTTTCCCCTACTAA
AJ505947.1
PE-Q-F
PE-Q-R
GCTTGCGTCTTTGACAACTCAGG
GTGCCACCACTGCATTCGCTAT
U50986.1
CEL2-Q-F
CEL2-Q-R
ACACATTGCCAAACGTCAGGT
CCCCTATGGTGAATCCTTTGTG
FB901714.1
EXP-Q-F
EXP-Q-R
CCCTCCTCGCCCTCACTTT
TCTGATTCCTCCTTGCTTTCG
U82123.1
MAN-Q-F
MAN-Q-R
ACACCGTCCTCCTGAGATTGG
GAGCCTCTGCTTTCCACTTTAATC
AF017144.2
TBG4-Q-F
TBG4-Q-R
AAATGGTGAAGGCGTAGGTCG
AGGTTGTCCGCAGTTAGTCTGG
AF020390.2
XTH5-Q-F
XTH5-Q-R
CCACCACCAGAGTGCGAGAT
TTTTCTTAGGATGACGATGTCCG
NM_001247541.2
XYL-Q-F
XYL-Q-R
TGATCGGCAATTATGAAGGTATTC
CAGCACATCCTGGCTTGTAAAT
AB041811.1
