Supplementary material

New procedure of quantitative bioimaging of Ti and Al released from dental implant and Mg, Ca,

Fe, Zn, Cu, Mn as physiological elements in oral mucosa by LA-ICP-MS

1Adam Sajnóg, 1Anetta Hanć, 2Ryszard Koczorowski, 1Danuta Barałkiewicz

1Department of Trace Element Analysis by Spectroscopy Method, Faculty of Chemistry, Adam

Mickiewicz University in Poznan, Umultowska 89b, 61-614 Poznan, Poland

2Clinic of Geriatric Dentistry, Karol Marcinkowski University of Medical Sciences in Poznań,

Bukowska 70, 60-812 Poznan, Poland

Corresponding Author: Danuta Barałkiewicz

E-mail address: danutaba@amu.edu.pl (D. Barałkiewicz)

Tel.: +48 61 829 15 73, fax: +48 61 829 15 55

Table S.1 Selected potential polyatomic interferences forming in the argon plasma

Isotope (abundance [%]) Theoretical polyatomic interferences26Mg (11.01) 12C14N+, 12C2

1H2+, 13C2

+

27Al (100) 13C14N+, 12C15N+

43Ca (0.14) 27Al16O+

49Ti (5.41) 36Ar13C+, 36Ar12C1H+, 32S17O+, 12C37Cl+, 14N35Cl+, 33S16O+, 31P18O+

55Mn (100) 40Ar14N1H+, 40Ar15N+, 38Ar17O+, 36Ar18O1H+, 23Na32S+, 37Cl18O+, 39K16O+

57Fe (2.12) 40Ar17O+, 40Ar16O1H+, 40Ca16O1H63Cu (69.17) 40Ar23Na+, 47Ti16O+, 23Na40Ca+

66Zn (27.90) 38Ar14N2+, 33S2

+, 50Ti16O+,48Ti18O+, 34S16O2+,32S16O18O+

Fig. S.1 Signal of 24Mg after ablation of NIST 610 glass standard in standard ablation chamber (a)

and in modified chamber with lower volume (b) resulting in faster wash-in and wash-out of the

aerosol, sharper signal and reduced tailing effect

Fig. S.2 Sinals measured after ablation of solid salts of ammonium sulfate, sodium chloride,

methionine, ammonium phosphate and cysteine in order to verify the presence of polyatomic

interferences originated from Ar, N, O, P, Na, Cl, S and H. Dwell times for 31P and 23Na are set to

10 ms and 5 ms respectively.

Fig. S.3 Signals measured after ablation of clean PET base and layer of O.C.T. cooling medium

on PET used to immobilise samples in crysotat. Signal of 121Sb contained in PET appear which

served as a indicator of complete ablation of a sample. Dwell time for 121Sb was set to 5 ms.

Fig. S.4 LA-ICP-MS analysis of titanium implant: a) intraosseous part made of Ti which was

sandblasted with Al2O3, b) abutment made of pure Ti and c) cover screw placed in the abutment

of implant for the healing period made of pure Ti. The LA parameters were the same as in oral

mucosa analysis. Signals of other elements (Mg, Ca, Fe, Zn, Cu, Mn) were at the background

level.

Fig. S.5 Maps of distribution of Ti, Al and building (C, S, P) and physiological (Mg, Ca, Fe, Zn,

Cu, Mn) elements in the oral mucosa sample of control group. Photographs after HE staining is

presented.

Fig. S.6 Maps of distribution of Ti, Al, Mg, Ca, Fe, Zn, Cu and Mn in the oral mucosa sample

collected from patient after implantation procedure. Elements derived from implants (Ti, Al),

physiological (Mg, Ca, Fe, Zn, Cu, Mn) and building (C, S, P) elements of oral mucosa and

photographs after HE staining are presented.

Fig. S.7 Maps of distribution of Ti, Al, Mg, Ca, Fe, Zn, Cu and Mn in the oral mucosa sample

collected from patient after implantation procedure. Elements derived from implants (Ti, Al),

physiological (Mg, Ca, Fe, Zn, Cu, Mn) and building (C, S, P) elements of oral mucosa and

photographs after HE staining are presented. Close-ups of areas with visible particles are

provided.