Supplementary Materials for:

Palladium/iron nanoparticles stimulate tetrabromobisphenol A microbial

reductive debromination and further mineralization in sediment

Authors:

Xiao-Qiu Lina, Zhi-Ling Li*a, Ying-Ying Zhua, Fan Chena, Bin Liangb, Jun Nana, Ai-

Jie Wanga,b

Affiliation:

a State Key Laboratory of Urban Water Resources and Environment, School of

Environment, Harbin Institute of Technology, Harbin 150090 China;

b Key Laboratory of Environmental Biotechnology, Research Center for Eco-

Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, P.R. China;

*Corresponding author: Professor. Zhi-Ling Li

E-mail: lzlhit@163.com

TEL: +86-451-86286840

Pages: 7

Figures: 3

Tables: 3

1

Cl

(A) (B)

(C) (D)Fe Pd

Figure S1. The morphology of the particles surface and the analysis of Fe and Pd

distributions through SEM-EDS in SS-Pd/Fe (0.412 g L-1 dosage, 0.5 wt% Pd

loading). (A) Proportions of Pd and Fe; (B) The morphology of the particles and the

elemental distributions of Pd (red), Fe (green), C (yellow) and O (blue); (C) and (D)

The elemental distributions of Fe and Pd, respectively.

2

710 720 730 7402000

4000

6000

8000

10000

Fe3+

Fe0

Inte

nsity

(a.u

.)

B.E.(eV)

Fe3+

332 336 340 344 348

1100

1200

1300

1400

Inte

nsity

(a.u

.)

B.E.(eV)

Pd 3d5/2Pd 3d3/2

(A) (B)

Figure S2. X-ray Photoelectron Spectroscopy (XPS) analysis for particles in SS-Pd/Fe

(0.412 g L-1 dosage, 0.5 wt% Pd loading) after TBBPA decomposition. (A) Fe spectra

from XPS analysis; (B) Pd spectra from XPS analysis.

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(A) (B)

Figure S3. The overall networks revealing the interactions between OTUs from the

bacterial communities in SS (A) and SS-Pd/Fe (B). A blue line indicated a positive

interaction between two individual nodes, while, a red line indicated the negative

interaction.

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Table S1: TBBPA removal efficiency (R10) at 10 d and the rate constant (k) in SS and SS-Pd/Fe fed with the different dosages and Pd loadings

Different dosages of Pd/Fe nanoparticles (1.0 wt% Pd loading, g L-1) Different Pd loading (0.412 g L-1 Pd/Fe, wt %)

0 0.0412 0.206 0.309 0.412 1.03 2.06 4.12 0.1 0.5 0.8 1.0

R10

(%)

60.9964.81 74.39 87.51 100 100 100 100 100 100 100 100

k a 0.10±0.01 0.12±0.01 0.14±0.01 0.20±0.01 0.47±0.02 0.47±0.01 0.63±0.02 0.61±0.01 0.27±0.01 0.42±0.01 0.46±0.02 0.46±0.02

r2 b 0.996 0.992 0.994 0.990 0.995 0.998 0.999 0.999 0.991 0.997 0.993 0.999

a: k indicated the first-order fitting kinetic constants for TBBPA decomposition;

b: r2 indicated the fitted linear regressions

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Sample

ID

Seq

num

OTU

Num*1

Shannon

index

ACE

index

Chao1

indexCoverage Simpson

S1 46860 2150 4.60 2912.18 2792.72 0.98 0.06

S2 43667 2073 4.83 2852.98 2766.94 0.98 0.04

S3 49240 2021 3.90 2809 2611.30 0.99 0.11

SP1 50135 2138 4.59 2924.45 2797.71 0.99 0.05

SP2 54032 2188 4.56 3078.71 2920.19 0.99 0.04

SP3 48564 2088 4.78 2963.38 2863.12 0.98 0.03

Table S2. Microbial diversity indexes of identified OTUs for bacteria in the samples of SS (S1, S2, S3) and SS-Pd/Fe (SP1, SP2, SP3) through

the high-throughput sequencing.

*1: “OTU num” indicated the classified OTU numbers obtained from the gene sequences with the identity of over 97 %.

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Table S3: Major topological properties of the empirical networks for the bacterial community in SS and SS-Pd/Fe.

Empirical networks

Microbial

communities

Network

size aLinks

Averaged

connectivity

Averaged

path

length

Averaged

clustering

coefficient

Modularity

(fast greedy)

SS 306 1416 9.26 7.25 0.54 0.62

SS-Pd/Fe 324 1006 6.21 10.52 0.54 0.74

a: The number of nodes in the network

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