Arizona Molecular Diagnostic & Research Laboratories

Robotics 2006

Shared Needs

MissionTo provide clinically relevant molecular laboratory services for our patients, providers, and research staff through an integration of laboratory practice, research, and education.

Service:

Education:

Research:

Joint Venture

A collaboration of clinical and anatomic laboratories, designed to support molecular diagnosis of disease, provide education, and perform research

Clinical Laboratory Directors

Ronald Schifman, M.D. Ronald Weinstein, M.D.

Donna Wolk, Ph.D. Anil Prasad, M.D.

Margaret Rennels, M.D. Lisa Rimsza, M.D.

Monika Schmelz, Ph.D. Christopher Cuniff, M.D.

AMDRL Translational Services Core Services, GLP

Nucleic Acid Extraction Real-time PCR: QPCR and SNP SNP Analysis with Invader Assays Fluorescent Microscopy with Digital

Imaging/Fluorescent In-Situ Hybridization

Molecular Epidemiology, rep-PCR Confocal Laser Scanning Microscopy Immunohistochemistry (digital scanning) Biorepository Services Misc. Clinical Laboratory Testing

GLP Research Core

MagNA Pure LC DNA Extraction

Boom extraction technology adaption

Lysis in chaotropic salts and proteinase K DNA bound to MGP’s, washed, & eluted

32 samples / 1-2 hrs.

Real-time PCR: ABI Sequence Detection System, 7900 HT, Taq 48 for viral loads

Molecular Epidemiology: Rep-PCR

Also T-cell clonality

Invader Platform: SNPFVL, FII, HCV genotype

                                 

Microscopy & DNA Probes for Cancer (FISH)

Her-2-Neu positive

Confocal Laser Scanning Microscopy

Focal Contacts:VinculinActin (Phalloidin-FITC)

Opportunity: MAVERIC

National VA Biorepository Processing, Storage, and Analysis Consent Process Technical Infrastructure Space and Infrastructure at VA Software System, TissueBase

                   

Current Status Providing infrastructure so that the latest

technology can be acquired and used for diagnosis and clinical research purposes

Performing funded clinical research Bioterrorism, Waterborne pathogens Sepsis Valley Fever Community Acquired Pneumonia Drug-resistant pathogens Prostate Cancer and Bladder Cancer GI Cancer Skin Cancer

Current Status Clinical Lab assays

Mycobacterium sp. By DNA probe Coccidioides by DNA probe and real-time

PCR HCV real-time PCR HIV RT-PCR Molecular epidemiology, ORSA, VRE, others CT/NG Microsporidia by real-time PCR Factor V Leiden Prothrombin gene (Factor II) FISH for bladder cancer FISH for Her-2-neu

Thank you