Areation of Cereal Dough by Yeast-Lak

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    SUGAR BOILING

    Name: T.L.V.Peiris

    Index No: GS/MSc/Food/3630/08

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    MATERIALS

    Measuring cylinders [500 ml]

    Graduated pipettesConical flasks [500 ml]

    Stoppers for conical flasks

    Rubber tubingWater bath [40 0 C]

    Perforated plungers

    Mixing bowls

    SpoonsWeighing scale

    INGREDIENTS

    Wheat flour 1500 g

    Rice flour 150 gCorn starch 150 g

    Malt 6. 75 g

    20 % sodium chloride 135 ml

    Potassium bromideSodium meta bisulphite [S M S]

    YEAST - yeast Mauripan [instant dry yeast ]

    PROCEDURE

    THE EFFECT OF YEAST CONCENTRATION ON GAS PRODUCTION

    The wheat flour was taken and three doughs [A, B, C ] were made based onthe following formulae by changing only the yeast concentration.

    Water was added in such a way to make the total volume constant

    .DOUGH

    A B C

    flour 50 g 50 g 50g

    Malt 0.25g 0.25 g 0.25g

    20 % salt solution 5 ml 5 ml 5 ml

    20 % YEAST suspension 5 ml 10 ml 15 ml

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    Water 22 ml 17 ml 12 ml

    All above ingredients were measured accurately and mixed using separate spoons inmixing bowls kneading with fingers to make the doughs with properly mixed

    ingredients. Those three doughs were put into 500ml conical flasks.

    A large plastic container and filled with water was taken.Three 500ml measuring cylinders were filled with water and were inverted in

    that water container without trapping of air bubbles .

    The three conical flasks which were prepared previously were connected to themeasuring cylinders using rubber tubes , and wax was applied to the connecting ends

    to prevent escape of gas.

    The volume of accumulated gas in the measuring cylinders were recorded every 15minutes until no more gas production was observed.

    A graph was plotted with the results obtained.

    RESULTS

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    Time /min Accumulated gas volume/cm3

    A B C

    15 - - 25

    30 - 20 85

    45 - 35 165

    60 - 45 230

    75 15 60 275

    90 20 70 305

    105 25 70 320

    120 30 70 320135 40 70 320

    150 40 70 320

    165 40 70 320

    180 40 70 320

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    Effect of Yeast Concentration on Gas Production

    0

    50

    100

    150

    200

    250

    300

    350

    Time(m

    in)

    1

    5

    3

    0

    4

    5

    6

    0

    7

    5

    9

    0

    10

    5

    12

    0

    13

    5

    15

    0

    16

    5

    Time (min)

    GasAccumulation(

    EFFECT OF TEMPERATURE ON GAS PRODUCTION

    PROCEDURE

    A mixture was prepared and dough was made according to the formula B

    ;ie; Flour 50gMalt 0.25 g

    20 % salt solution 5 ml

    20 % yeast suspension 10 mlWater 17 ml

    This dough was put into a 500 ml conical flask and connected to a 500ml measuringcylinder that was immersed in water , using rubber tubing and the connecting ends

    were sealed with wax.

    The conical flask which contained the dough was put into a 40 o C water bath.

    The temperature was kept constant at 40 0 C in the water bath throughout the

    process and was conformed it using a thermometer .

    The gas production of the dough was measured every 15 minutes until there wasno more gas production.

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    Effect of Tem perature on Gas Prod

    0

    50

    100

    150

    200

    250

    Time(min) 1

    5 30 45 60 75 90 105

    120

    135

    150

    165

    Time (mim

    Gas

    Acumulation(ml)

    B @ 3

    B1 @

    EFFECT OF MALT EXTRACT

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    Time /min Accumulated gas volume/cm3

    B (Room

    temperature)

    B1(at 40oC)

    15 - 25

    30 20 40

    45 35 85

    60 45 100

    75 60 11090 70 140

    105 70 195

    120 70 220

    135 70 220

    150 70 220

    165 70 220

    180 70 220

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    PROCEDURE

    A dough was prepared using the ingredients in formula B but instead of malt

    extract corn flour was used, so that the formula was as below,

    Wheat flour 50g

    CORN FLOUR 0.25g

    20 salt solution 5 ml20 yeast suspension 10ml

    Water 17 ml

    The dough was kept in 500 ml conical flask and the apparatus was connected as

    before and kept for gas accumulation.

    The collection of gas was measured every 15 minutes until it reached a constant

    value .

    RESULTS

    Time/min Accumulated gas volume/cm3

    B (with corn) B11(with malt)

    15 - -

    30 20 -

    45 35 10

    160 45 20

    75 60 25

    90 70 25105 70 25

    120 70 25

    135 70 25

    150 70 25

    165 70 25

    180 70 25

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    Effect of Malt Extract

    0

    10

    20

    30

    40

    50

    60

    70

    80

    Time(mi

    n)

    15

    30

    45

    60

    75

    90

    10

    5

    12

    0

    13

    5

    15

    0

    16

    5

    Time (min)

    GasAccumulation(ml)

    B with malt

    B II without m

    EFFECTS OF SALT CONCENTRATION ON GAS PRODUCTION

    PROCEDURE

    Three doughs were prepared using following formulas and was put into 500ml conical flasks.

    A B C

    Flour 50g 50g 50g

    Malt 0.25g 0.25g 0.25g

    20SALT SOLUTION 0 5ml 10 ml20yeast suspension 10 ml 10ml 10ml

    Water 22ml 17ml 12ml

    The water was added to have a same volume in all three doughs.

    Those three flasks were connected to 500ml measuring cylinders and gas wasallowed to collect .

    The gas that was released was measured until there was no more accumulation

    noted and a graph was plotted using the results.

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    RESULTS

    Effect of Salt Concentration

    0

    50

    100

    150

    200

    250

    300

    350

    400

    Time(min)

    15 30 45 60 75 90 105

    120

    135

    150

    165

    Time (min)

    GasAccum

    ulation(ml)

    A with No S

    B with 5ml s

    C with 10ml

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    Time /min Accumulated gas volume/cm3

    A B C

    15 10 130 05

    30 25 190 1545 35 250 25

    60 50 300 40

    75 75 330 50

    90 100 340 50

    105 130 350 50

    120 130 350 50

    135 130 350 50

    150 130 350 50

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    GAS RETENTION

    EFFECT OF PROTEIN CONTENT AND FLOUR TYPEThe following doughs were made using the following formulas and were put in to

    500ml measuring cylinders .

    D1 D2 D3

    Wheat flour 50g ---- -----Glutan + wheat flour --- 50g -----

    Rice flour --- ----- 50g

    Malt 0.25g 0.25g 0.25g

    20 % salt solution 5ml 5ml 5ml20% yeast suspension 15 ml 15 ml 15 ml

    Water 12 ml 12ml 12ml

    The doughs were covered with paraffin oil and perforated plungers were puton top of the dough after making doughs to immerse in oil.

    The initial oil levels above the each dough was measured.

    The doughs were kept at room temperature and the oil levels were measured in

    every 15 minutes until a constant level was achieved.A graph was plotted using the results.

    RESULTS

    The oil levels in each dough

    D1 D2 D3

    Initial oil levels 150 ml 150 ml 250 ml

    Time [minutes ]

    15 250 ml 250ml 250 ml30 275 ml 270 ml 250ml

    45 275 ml 280 ml 250ml

    60 275 ml 290 ml 250 ml75 280 ml 290 ml 250 ml

    90 280 ml 300 ml 250 ml

    105 280 ml 300 ml 250 ml120 280 ml 300 ml 250 ml

    135 280 ml 300 ml 250 ml

    150 280 ml 300 ml 250 ml

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    CALCULATION

    The gas retention [ml] in each dough was ,

    D1 D2 D3Time [minutes]

    15 100 100 0

    30 125 120 045 125 130 0

    60 125 140 0

    75 130 140 0

    90 130 150 0105 130 150 0

    120 130 150 0

    135 130 150 0

    150 130 150 0

    Gas Retention - Effect of Protein Contect and Flour type

    0

    20

    40

    60

    80

    100

    120

    140

    160

    Time(min)

    15 30 45 60 75 90 105

    120

    135

    Time (min)

    GasRetention(ml)

    D1(With Wheat)

    D2 (Gluten +Whe

    D3 ( Wth Rice Flo

    As the gas that formed cannot escape through the oil layer , it get trapped inside

    the dough causing the oil level to move up. So the oil level indicates the

    gas retention ability of the dough with time.

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    EFFECT OF ADDITIVES ON GAS RETENTION

    PROCEDURE

    The following ingredients were measured accurately .

    A BWheat flour 50 g 50g

    Malt 0.25g 0.25 g

    20 % yeast suspension 10 ml 10 mlWater 17 ml 17 ml

    0.05 g potassium bromide was taken and dissolved in 5 ml of 20 % salt

    solution and was added to the mixture A .0.05 g sodium metabisulphite [S M S ] was measured and dissolved in 5 ml of

    20% salt solution and was added to the mixture B.

    Two doughs were made using the above A and B mixtures and were put into

    500 ml measuring cylinders as before .The doughs were covered using paraffin oil placing the perforated plunger on top of

    the dough. The initial oil levels were measured.The doughs were kept at room temperature , and the oil levels were measured in

    every 15 minutes until there was no more change observed.

    A graph was plotted using the results.

    RESULTSOil levels [ml]

    A [KBr] B [S M S]

    Initial oil level 200 150

    Time [minutes ]

    15 200 150

    30 230 20045 260 205

    60 275 210

    75 285 21590 285 215

    105 285 215

    120 285 215135 285 215

    150 285 215

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    DISCUSSION

    Not only the formation but also the retention of gas is important in bakery industry.

    This character is mainly determined by the quantity and the quality of the glutan

    present in the dough. The glutan proteins namely glidin and glutenin are presentseparately in the flour and interact to form glutan with hydration and physical

    manipulation during dough formation .

    In this practical more gas retention was obtained with wheat flour , which containmore glutan than rice flour and even more gas retention in gluten added wheat

    flour.

    Out of the additives the KBr is a dough improver and SMS is a preservative.

    The bromide added is converted to bromine and act as an oxidizer and made thedough with high air retention capacity. SMS protect the dough from staling hence

    exert a preservative action .So higher air retention capacity was observed in KBr.

    Chemical Leavening Agents

    The interaction of water, acid, heat and chemical leavening agents (baking powders)releases CO2. The release of gas may occur in the dough prior to or during oven baking.

    The agents consist of a CO2-generating source, as a rule sodium bicarbonate,

    and an acid carrier, usually disodium dihydrogendiphosphate, sometimes monocalcium

    phosphate [Ca(H2PO4)2]. Glucono--lactone or tartar (acidic potassium tartrate) are usedas acid carrier for phosphate-free baking powder. The phosphate-free leavening mixtures

    produced on an industrial scale contain citric acid or its acidic sodium salt. In baking

    powder, the two reactive constituents are blended with a filler which consists of corn,rice, wheat or tapioca starch or sometimes dried wheat flour. The filler content in

    the powder is up to 30%. The role of the filler is to prevent premature release of CO2.

    The market also offers baking powders flavored with vanillin or ethyl vanillin.For every 500 g of flour, baking powder should develop 2.352.85 gCO2, equivalent to

    about 1.25 liters. In individual cases, NaHCO3 alone is used for some flat shelf-stable

    cookies and ammonium hydrogencarbonate (NH4HCO3) for many others. Ginger andhoney cookies are leavened by NH4HCO3, mostly together with potash (K2CO3). To a

    small extent, a 1:1 mixture of ammonium hydrogencarbonate and ammonium carbamate

    (H2NCOONH4) is used in some countries.Both decompose above 60 C to NH3,CO2

    and water.

    References

    1. Food processing technology Principles and Practice Second Edition P. Fellows

    Director, Midway Technology and Visiting Fellow in Food Technology at Oxford

    Brookes University2. Food Chemistry H.-D. Belitz W. Grosch P. Schieberle 4th revised and extended

    ed.

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