Are Dropped Osteoarticular Bone Fragments Safely Reimplantable in Vivo?

by Benjamin Bruce, Shahin Sheibani-Rad, Deborah Appleyard, Ryan P. Calfee, Steven E. Reinert, Kimberle C. Chapin, and Christopher W. DiGiovanni

J Bone Joint Surg AmVolume 93(5):430-438

March 2, 2011

©2011 by The Journal of Bone and Joint Surgery, Inc.

Diagram illustrating the distribution of specimens in the coagulase-negative Staphylococcus (CNS) arm in Phase II. The 140 specimens were split into five equal groups of twenty-eight

specimens to be treated with povidone-iodine, 4% chlorhexidine gluconate, ...

Benjamin Bruce et al. J Bone Joint Surg Am 2011;93:430-438

©2011 by The Journal of Bone and Joint Surgery, Inc.

Diagram illustrating the treatment protocol for each of the groups in the coagulase-negative Staphylococcus (CNS) arm in Phase II. Each specimen was treated in a chemical bath for either

five or ten minutes.

Benjamin Bruce et al. J Bone Joint Surg Am 2011;93:430-438

©2011 by The Journal of Bone and Joint Surgery, Inc.

Bar graphs showing the results in Phase I. The top figure shows that 113 (70%) of 162 grafts that were dropped on the floor had positive cultures.

Benjamin Bruce et al. J Bone Joint Surg Am 2011;93:430-438

©2011 by The Journal of Bone and Joint Surgery, Inc.

Representative fluorescent photomicrographs of stained cartilage cells from specimens in Phase III, made with use of a confocal microscope.

Benjamin Bruce et al. J Bone Joint Surg Am 2011;93:430-438

©2011 by The Journal of Bone and Joint Surgery, Inc.

Box plot illustrating cell viability after decontamination.

Benjamin Bruce et al. J Bone Joint Surg Am 2011;93:430-438

©2011 by The Journal of Bone and Joint Surgery, Inc.