APPLIED MICROBIOLOGYVOLUME 19 * NUMBER 6 * JUNE 1970

EDITORIAL BOARD

J. C. Sylvester, Editor-in-Chief (1972)Abbott Laboratories, North Chicago, lll.

Albert Balows, Editor (1972)National Communicable Disease Center,

Atlanta, Ga.

Marvin P. Bryant, Editor (1971)University of Illinois, Urbana

Ralph N. Costilow, Editor (1970)Michigan State University, East Lansintg

Oldrich K. Sebek, Editor (1972)The Upjohn Co., Kalamazoo

R. F. Acker (1972)Milton J. Allison (1971)A. W. Anderson (1972)Nester Bohonos (1970)Francis F. Busta (1972)Alex Ciegler (1971)Rita R. ColweU (1971)Marjorie A. Darken (1970)R. H. Deibel (1972)A. L. Demain (1971)R. N. Doetsch (1972)John Ehrlich (1971)James D. Fenters (1970)William W. Ferguson (1971)Maxwell Finland (1972)Anthony F. Gaudy, Jr. (1970)S. E. Gilliland (1971)T. C. Grubb (1970)H. Orin Halvorson (1970)

Clifford W. Hesseltine (1971)William L. Hewitt (1972)John J. landolo (1971)Henry D. Isenberg (1971)Marvin J. Johnson (1972)S. S. Kalter (1972)Alfred G. Karlson (1972)Leo Kaufman (1972)Lloyd L. Kempe (1970)George E. Kenny (1970)William M. M. Kirby (1972)W. A. Konetzka (1970)Hubert Lechevalier (1970)Joseph Lein (1972)John Liston (1971)Warren Litsky (1972)Robert Mah (1971)Joseph L. Melnick (1970)William I. Metzger (1971)

C. F. Niven, Jr. (1970)David Paretsky (1970)D. Perlman (1971)David Pramer (1971)John B. Routien (1971)Carl P. Schaffner (1972)E. L. Schmidt (1971)Warren S. Silver (1970)Louis DS. Smith (1972)S. S. Socransky (1972)Christian Stoli (1970)Welton I. Taylor (1970)M. J. Thirumalachar (1970)Joseph P. Truant (1970)Marvin Turck (1971)Hamao Umezawa (1970)George H. Warren (1972)Eugene D. Weinberg (1972)Max R. Zelle (1971)

Robert A. Day, Managing Editor, 4715 Cordell Ave., Bethesda, Md. 20014

EX OFFICIO

E. M. Foster, President (1969-1970)Donald E. Shay, Secretary

Applied Microbiology, a publication of the American Societyfor Microbiology, 4715 Cordell Ave., Bethesda, Md. 20014, isdevoted to the advancement and dissemination of applied knowl-edge concerning microorganisms. Applied Microbiology is pub-lished monthly, two volumes per year. The subscription price is$30 (foreign, $31) per year. Single copies are $4.00 (forcign,$4.25). Members of the American Society for Microbiology mayreceive Applied Microbiology as part of their dues. Correspond-ence relating to reprints, defective copies, availability of backissues, anid lost or late proofs should be directed to the ASMPublications Office, 4715 Cordell Ave., Bethesda, Md. 20014.Correspondence from ASM members relating to membership

R. E. Hungate, Vice-President (1969-1970)H. B. Woodruff, Treasurer

dues, member subscriptions, changes of address, incorrect addressincorrect journals, etc., should be directed to the American Societyfor Microbiology, 1913 I St., N.W., Washington, D.C. 20006.Nonmembers of the Society should write to The Williams &Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202, regardinginstitution ofnonmember subscriptions and nonreceipt of journals.Published monthly by the ASM at 428 E. Preston St., Baltimore,Md. 21202.

Second-class postage paid at Baltimore, Md. 21202, and at addi-tional mailing offices. Made in the United States of America.Copyright @ 1970, American Society for Microbiology.All Rights Reserved.

1

Author IndexAbernathy, Robert S., 995Anna, E. D., 890

Babcock, G. E., 1019Bates, Joseph H., 955Beesley, Earl D., 915Bolin, Richard C., 894Braymen, D. T., 1021Brown, J. P., 1001

Carlisle, Harold N., 943Carver, Russell K., 894Coler, Robert A., 1009

Darby, Richard T., 1007Diena, B. B., 1025Dixon, Glen J., 950Duncan, Charles L., 980Duncan, William P., 898

Easterbrook, K. B., 997Ehrlich, H. L., 966Elliker, P. R., 883

Fields, Branch T., Jr., 955Forziati, Florence H., 950Foster, Edith F., 980Freeburg, Pat W., 940

Gerone, Peter J., 963Gilliland, S. E., 890Green, James H., 894Greenberg, L., 1025

Gross, Herman, 894Gunner, Haim B., 1009Gupta, K. G., 1017

Hareven, D., 1005Harrell, William K., 894Hensley, D. E., 1019Himmelfarb, Philip, 1013Holm, H. W., 988

Jay, James M., 902

Kenny, C. P., 1025Keogh, Barbara P., 928Kessar, S. V., 1017Koltin, Y., 1005

Lavender, J. F., 923Ledward, D. A., 937Lewis, Joel S., 898

McDade, Joseph E., 963Maier, S., 1023Matsen, John M., 1015Mizrahi, A., 906Moore, G. E., 906

Nicol, D. J., 937

Pigott, Nan, 894

Raymond, E. A., 919Robbins, Ruth N., 911

Roinestad, Frank A., 973Rossi, C. R., 932Rozee, K. R., 997

Safranski, M. J., 1019Sandine, W. E., 883Saslaw, Samuel, 943Scott, Arthur, 1013Seki, Humitake, 960Shaw, M. K., 937Shelef, Leora A., 902Shovers, J., 883Sidwell, Robert W., 950Singh, Baldev, 1017Slodki, M. E., 1019Songer, J. R., 1021Speck, M. L., 890Stemper, Joanne E., 1015Stout, Genevieve W., 898Strong, Dorothy H., 980Surgalla, Michael J., 915Thayer, Philip S., 1013Thompson, Janet W., 883Traub, W. H., 919Trimble, R. B., 966

Vennes, J. W., 988

Wallace, R., 1025Watrach, A. M., 932Weiss, K. F., 911Westbrook, Louise, 950Yall, Irving, 973

APPLIED MICROBIOLOGY

INSTRUCTIONS TO AUTHORS

Correspondence. Submit manuscripts in duplicate(original and one carbon) to ASM Publications Office,4715 Cordell Ave., Bethesda, Md. 20014.

General policy. Any manuscript submitted must bea report of unpublished original research, which is notbeing considered for publication elsewhere. A manu-script accepted and published by Applied Microbiol-ogy must not be published again in any form withoutthe consent of ASM.A charge of $25 per printed page is assessed for pub-

lication. Most institutions and granting agencies inthe United States permit the payment of publicationcharges as a part of their general research support.It is recognized, however, that such funds are not al-ways available to laboratories outside the UnitedStates. In such cases, the cost of publication will beborne by the American Society for Microbiology.The "editorial style" of Applied Microbiology essen-

tially follows the Style Manual for Biological Journals(2nd ed., AIBS, 1964). Genetics symbols should essen-tially follow the recommendations of Demerec et al.(Genetics 54:61, 1966). Biochemical abbreviationsshould essentially follow the rules of the IUPAC-IUB(J. Biol. Chem. 241:527, 1966). Enzyme activity shouldbe expressed in terms of international units (EnzymeNomenclature, Elsevier Publishing Co., 1965), and theEC number should be given parenthetically at first usein the text. In expressing lengths, weights, and vol-umes, the prefixes nano (n) and pico (p) should beused instead of millimicro (m,u) and micromicro (,u,u).Express lengths in nanometers (nm; 10-- m) or in mi-crometers (,Am; 10-6 m) instead of millimicrons (m,u;I0 m), microns (,u; 10-6 m), or Angstroms (A;10-10 m). Express parts per million (ppm) as mi-crograms per milliliter (,Ag/ml), micrograms pergram (,ug/g), or microliters per liter (.uliters/liter), asappropriate. Applied Microbiology reserves the privi-lege of editing manuscripts to make them conformwith the adopted style.Form of manuscript. Manuscripts should be typed

on bond paper (8/1t by 11 inches). All parts of themanuscript should be typed double-space or, prefer-ably, triple-space with margins of at least I j1 inchesat the top, bottom, and both sides of each page. Mostmanuscripts can and should be divided into the follow-ing sections: Abstract, Introduction, Materials andMethods, Results, Discussion, Acknowledgments, andLiterature Cited. Only one weight of heading (para-graph lead-in) should be used within each section.

Abstract. An Abstract appears at the beginning ofeach paper. The Abstract should not exceed 250words.

Literature Cited. In the text, references are cited bynumber. The Literature Cited section should be typedin alphabetical order, by first author, and numbered.Names of journals are abbreviated according toAccess (American Chemical Society, 1969). Literaturecitations should be restricted to closely pertinent pa-pers. Citations of abstracts, theses, "unpublisheddata," "personal communication," and "in press"will not be accepted in the Literature Cited.

Tables. Each table should be typed on a separate

page. The data should be arranged so that columns oflike material read down, not across. The headingsshould be sufficiently clear so that the meaning of thedata will be understandable without reference to thetext. Explanatory footnotes are permitted, but detaileddescriptions of the experiments are not. The materialsand methods used to gain the data should properlyremain in the section of that name.

Figures. A complete set of figures, preferably glossyphotographs, should accompany each of the twocopies of the manuscript. Graphs (submit as photo-graphs) should be finished drawings not needingfurther artwork or type-setting. Figures and graphsshould not exceed 8)it by 11 inches. Absolutely n1o partof a graph should be typewritten (except the legendwhich should be typed on a separate page). Each fig-ure should be numbered and should include the nameof the author, either in the margin or on the back(marked lightly with a soft pencil). All letteringshould be done with a lettering set. Most graphswill be reduced to 2% inches in width, and all elementsin the drawing should be prepared to withstand thisreduction. The legend of the figure should provideenough information so that the figure is understand-able without reference to the text. Experimental de-tails from Materials and Methods should not berepeated in figure legends.

Nomenclature of microorganisms. In general, thenomenclature for bacteria presented in Bergey'sManual of Determinative Bacteriology (7th ed., 1957)is used. If an author challenges this nomenclature,his own judgment will be followed, but the name inBergey's Manual should follow in parentheses thefirst time the name is used in the text and in theAbstract. When a new bacterial name is proposed ina manuscript, an international authority on nomen-clature will be consulted for an opinion. When a newspecies, or a new variety of a species, is proposed,an acceptable photomicrograph or electron micro-graph of the cells should be submitted. If the cells aremotile, the photomicrograph or electron micrographshould show the nature and arrangement of flagella.As one of the requirements for description of a newspecies, we require deposition of the type culture in arecognized culture collection and designation of theaccession number.

Notes. The accepted form for Notes is somewhatdifferent from the foregoing. Contributors shouldconsult a recent issue of Applied Microbiology forstyle. Notes should not exceed 500 words. The ab-stract should not exceed 25 words.

Copyright. Once a paper has been published inApplied Microbiology, which is a copyrighted publica-tion, the legal ownership of all parts of the paper, in-cluding the illustrations, has passed from the authorto ASM. If the same author, or any author, wishes torepublish material previously published in AppliedMicrobiology, he must first receive written permissionfrom ASM.

Reprints. Reprints (in multiples of 100) may be pur-chased by contributors. A table showing the cost ofreprints, and an order form, will be sent with the proof.

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INDEX TO DATE OF ISSUE

Month Date of Issue Pages

January 1/28/70 1-206February 2/23/70 207-400March 3/31/70 401-556April 4/24/70 557-706May 5/25/70 707-882June 6/29/70 883-1025

APPLIED MICROBIOLOGY, June 1970, p. i-iiiCopyright © 1970 American Society for Microbiology

Al-THOR INDEX

VOLUME 19

Abernathy, Robert S., 955Adams, D. M., 878Adebayo, A. A., 536Affens, W. A., 76Akers, Thomas G., 624Akin, Elmer W., 805Alderton, Gordon, 565Aldova, Eva, 463Allen, M. R., 398Altemeier, William A., III, 785Amundson, C. H., 429Anders, R. F., 608Anderson, A. W., 73Anderson, E. L., 726Anna, E. D., 890Arioli, V., 704Armstrong, Charles H., 818, 823Armstrong, David H., 204Auernheimer, A. H., 521

Babcock, G. E., 1019Bader, J., 714Bahrami, S., 545Bailey, G. D., 232Baker, Carolyn N., 60Balish, Edward, 458Ball, Mary M., 694Baman, Sudhaker I., 474Bano, Zakia, 60Banwart, G. J., 768Barrett, Fred F., 345Barrile, Judith C., 177Barron, Almen L., 360Bartes, Sylvia F., 172Bates, Joseph H., 955Beesley, Earl D., 915Benner, E. Jack, 409Benton, William H., 805Bequette, R. J., 746Bissett, Marjorie L., 618Blazevic, Donna J., 134, 547Blenden, D. C., 586Bolin, Richard C., 894Bott, Thomas L., 100Boush, G. M., 879Brachman, Philip S., 401Braun, Peter, 62Braymen, D. T., 1021Breazeale, F. W., 379Brock, Thomas D., 100Brown, J. P., 1001Brown, John, 837, 842, 844Burger, Dieter, 549Burnett, J., 264Burns, Jean, 772Burton, Glenna, 586Busta, F. F., 878Butterworth, D., 718

Cabelli, V. J., 239Calisher, C. H., 557Camper, N. D., 379Carlisle, Harold N., 370, 943Carver, Russell K., 894Cerf, Olivier, 696Chamberlain, R. E., 776Chance, Britton, 446Chandler, Francis W., Jr., 326Chang, T. S., 220, 394Cherry, J. D., 658Chirigos, M. A., 867Chisholm, D. R., 14Cho, Cheng, T., 791Chung, K. C., 190Clark, H. F., 848Clark, John W., Jr., 326Cole, R. J., 106Coler, Robert A., 1009Cone, J. Frank, 177Cone, R. D., 138Cook, Elton S., 813Cox, C. S., 604Crawford, James J., 207Crowell, Clarence, 681Cundy, K. R., 268Currie, J. F., 701Curtis, P. R., 193

Dabbah, Roger, 27Darby, Richard T., 1007Das, N. K., 776Das, S., 598Davis, Richard B., 837, 842, 844Dawe, Donald L., 837, 842, 844Daye, George T., Jr., 731Dehority, B. A., 179Deig, E. Frank, 87Devine, Leonard F., 329DeWitt, Wallis E., 742Diena, B. B., 1025DiGioia, G. A., 451, 455Dixon, Glen J., 53, 950Dixon, J. M. S., 621Dondero, N. C., 663, 671Doupnik, Ben, Jr., 594Dubovi, Edward J., 624Duff, Michael F., 120Duncan, Charles L., 353, 980Duncan, William P., 898Dunkelberg, Wilbur E., Jr., 47Dunlap, R. C., 689Durand, Andrea M., 134

Easterbrook, K. B., 997Eckels, Kenneth H., 321Ederer, Grace Mary, 547Edwards, V. M., 27

Ehrlich, H. L., 966Ehrlich, Richard, 245Eidenbock, Marie P., 521Elander, R. P., 72Elliker, P. R., 649, 883Ellis, J. J., 103Epps, N. A., 338Erskine, J. M., 638, 707Ertola, Rodolfo J., 535Eveleigh, D. E., 872Ewing, W. H., 345

Fairchild, A. C., 875Feeley, John C., 742Felsenfeld, Oscar, 463Fields, Branch T., Jr., 955Finland, Maxwell, 345, 629Flanagan, Thomas D., 360Forziati, Florence H., 950Foster, Edith F., 980Foster, John W., 837, 842, 844Freeburg, Pat W., 940Fusillo, Matthew H., 425

Gammon, R. A., 146, 152, 157,163

Gangarosa, Eugene J., 434Gardner, W. R., 536Geiger, Paul J., 32Gerone, Peter J., 963Gettner, Seyedeh, 44Gilliland, S. E., 890Godfrey, J. C., 14Goepfert, J. M., 190, 429Goldberg, Herbert S., 505, 586Goldblith, S. A., 451, 455Goldsmith, D. J., 375Goldstein, Mark A., 290, 580Gordon, Ruth E., 862Gorman, M., 721Gourevitch, A., 14Green, James H., 894Green, Leonard, 196, 763Greenberg, L., 1025Gregory, J. E., 268Grocholski, J. J., 621Grosclaude, Gerard, 696Gross, Herman, 894Gruft, Howard, 199Gunner, Haim B., 1009Gupta, K. G., 1017

Hagerman, Cline R., 329Hahn, Ricardo G., 317Hahon, Nicholas, 224, 389Hall, M. J., 109Hamblet, Frederick E., 805Hamill, R. L., 721

Vol. 19, No. 6Printted in U.S.A.

AUTHOR INDEX

Hamilton, P. B., 392Hamlow, E. E., 746Hankins, William A., 224Hanlin, R. T., 128Hanscomb, G., 718Happich, William F., 53Hareven, D., 1005Harrell, William K., 894Harris, R. F., 536Harrison, D. E. F., 446Harrison, E. F., 746Harrison, Venton R., 170, 321Hartman, Paul A., 781Hartwell, W. V., 521Hassall, C. H., 109Hatch, M. T., 232Hatlen, Jack B., 317Hayes, Jean A., 186Heffernan, W. P., 239Heinemann, Bernard, 800Hendricks, Charles W., 441Henrikson, Duane M., 731Hensley, D. E., 1019Hesseltine, C. W., 542Hetrick, Frank M., 321Higgins, M. L., 527Hill, William F., Jr., 805Himmelfarb, Philip, 295, 1013Hobby, George L., 32Hochstein, H. Donald, 742Hodson, Phillip H., 551Hogg, D. M., 608Holaday, C. E., 106Holden, R. D., 182Holm, H. W., 988Holmes, James, 463Horowitz, Norman H., 32Horton, Richard E., 314Howard, Alma J., 800Hubbard, Jerry S., 32Huber, T. W., 383Hughes, David E., 201Hughes, M. Kathryn, 525Huisingh, D., 392

Ichinoe, Masakatsu, 613Idziak, Edmund S., 338Iskander, I. K., 429

Jago, G. R., 608, 875Jarvis, Audrey W., 698Jay, James M., 902

Kaminski, F., 848Karzon, D. T., 848Kashman, Y., 470Kass, Edward H., 62, 345Kaufman, Leo, 830Kellogg, Douglas S., Jr., 47, 287Kenny, C. P., 1025Kenny, George E., 317Keogh, Barbara P., 928Kereluk, K., 146, 152, 157, 163Kessar, S. V., 1017

Keudell, Kenneth C., 505Kinner, J. A., 768Kirskey, J. W., 106Klein, George C., 60Klein, Jerome O., 62Koehler, Judith A., 434Koltin, Y., 1005Kourany, Miguel, 694Kreider, John W., 174Kubica, G. P., 383Kundu, A. K., 598Kurata, Hiroshi, 613

Labbe, Ronald G., 353Lai, Mingtan, 542Lambert, M. A., 375Lane, A. G., 512Lanzilotta, R. P., 301, 307Lavender, J. F., 923Lawrence, R. C., 698Lechevalier, H. A., 527, 534Lechevalier, Mary P., 527, 534Lechowich, R. V., 138Ledward, D. A., 937Lennette, Edwin H., 491Lewis, Joel S., 898Ley, Kenneth D., 549Licciardello, J. J., 451, 455Lifshitz, A., 470Lillard, D. A., 128Lillard, H. S., 128Lillie, Mary G., 381Lin, Chau-Ching, 283Lin, Kuan-Chung, 283Lloyd, R. S., 146, 152, 157, 163Lo, P. W., 220Loaharanu, Paisan, 734Locke, Thomas, 791Loggins, Mary S., 855Lopez, Anthony, 734Love, John N., 700Lu, Wanda W.-W., 367Lucas, G. B., 271Lynd, J. Q., 11

Mabe, J. A., 721McCullough, Norman B., 314McDade, Joseph E., 963MacLeod, Robert A., 701McMinn, M. Talmage, 207Madden, David L., 314Maddox, Louise, 385Maier, S., 1023Males, J. R., 485Mallette, M. F., 367Maness, K. S. C., 557Marcovici, M., 398Marks, Melvin I., 184Martin, William J., 172Martin, William Jeffery, 694Martin, William Joseph, 259Martin, William T., 401Matsen, John M., 438, 547, 1015Matsumura, F., 879

Mazza, Luis A., 535Mercuri, A. J., 768Miller, Curtis E., 287Miller, John K., 772Miller, Sol, 245Miller, Stephen B., 583Mirchamsy, H., 545Misiek, M. 14Mizrahi, A., 906Moats, W. A., 27Mohanty, Sashi B., 381Moody, Max D., 5, 60Moore, G. E., 906Mora, Emilio C., 1Morelli, Frank A., 32Morris, George K., 401, 434,

795Moss, C. Wayne, 375Munden, J. E., 718Mundon, Francis K., 785Murphy, B. L., 521Murray, Don S., 11Myers, Donald M., 335

Natori, Shinsaku, 613Neeman, I., 470Nickerson, J. T. R., 451, 455Nicol, D. J., 937Noble, John, Jr., 855Noble, Robert C., 5Nutini, Leo G., 813Nutter, John E., 250Nygaard, Genevieve S., 618

Oberhofer, Thomas R., 385Ohtsubo, Kohichiro, 613Orcutt, D. M., 182Orr, Henry C., 387, 541, 580Orth, D. S., 73Osterhout, Mary, 199O'Toole, Elizabeth, 184

Palocz, Henry J., 800Pang, C. Hor-Nay, 862Pareilleux, A., 421Parrott, Marshall W., 463Patil, K. C., 879Payne, Jerry B., 818, 823Pearson, Ted A., 458Peckham, John C., 594Pigott, Nan, 894Pindak, Frank F., 188Pine, Leo, 413Pittillo, Robert F., 538Pradip, I. S., 193Prakasam, T. B. S., 663, 671Pramer, David, 301, 307Prauser, H., 527, 534Price, K. E., 14Probst, Peter G., 387Pugh, George W., Jr., 201Purser, D. B., 485

Raymond, E. A., 919Reber, H., 527

* X

APPL. MICROBIOL.

AUTHOR INDEX

Reddick, R. Anne, 383Reitman, Morton, 196, 763Rezai, Hamid R., 44Rice, J. M., 867Rice, N. R., 867Richardson, B., 182Rie, Ivo P., 87Rieck, Walter L., 11Robbins, Ruth N., 911Roberson, Bob S., 425Robinson, P. R., 398Rogers, Joseph L., 541Roinestad, Frank A., 973Rosner, Richard, 281Rossi, C. R., 932Rossi, E., 704Rozee, K. R., 997Russell, Philip K., 785

Safranski, M. J., 1019St. Julian, Grant, 681Saito, Mamoru, 613Sakaki, Setsuko, 613San Clemente, C. L., 214Sandine, W. E., 649, 883Saslaw, Samuel, 370, 943Savov, Zlatko, 113Schammel, Patricia, 287Schmale, John D., 287Schmidt, Nathalie J., 491Scott, Arthur, 1013Seki, Humitake, 960Semeniuk, G., 542Sessoms, A. R., 768Sevoian, Martin, 71Shafyi, A., 545Sharpe, E. S., 681Sharrar, Robert G., 434Shaw, M. K., 937Shelef, Leora A., 902Shelley, Gail, 392Shelton, William H., 401Shovers, J., 649, 883Shu, L., 193Shuler, R. L., 76Sicard, N., 421Sidwell, Robert W., 53, 950Simon, Gerald, 176

Simon, Harold J., 573Singh, Baldev, 1017Siniuk, Alicia A., 335Skaggs, Romulus, 47Slodki, M. E., 1019Smith, John P., 870Smith, Rodney F., 254, 643Snell, Neva, 565Solis, Juan, ISommers, L. E., 536Songer, J. R., 1021Sorrells, K. M., 39Speck, M. L., 39, 890Srivastava, H. C., 166Srivastava, K. K., 837, 842, 844Stegherr-Barrios, Anna, 463Stemper, Joanne E., 1015Stephens, J. F., 726Stewart, R. H., 865Stim, Thomas B., 751, 757Stout, Genevieve W., 898Strong, Dorothy H., 980Sukapure, R. S., 527Sutherland, R., 264Surgalla, Michael J., 915Sweet, Charles E., 830

Tanaka, Kinji, 813Tauraso, Nicola M., 290, 580Tavormina, P. A., 746Taylor, Gerald F., 131Taylor-Robinson, D., 658Telford, Sam R., Jr., 694Teske, Richard H., 583Teuber, Michael, 871Thayer, James D., 287Thayer, Philip S., 1013Thompson, Janet W., 883Toala, Publio, 629Tolls, T. N., 649Tookey, H. L., 103Top, Franklin H., Jr., 785Traub, W. H., 919Trenk, Hugh L., 781Tresner, H. D., 186Tribble, H. R., Jr., 763Trimble, R. B., 966Tsuchiya, Yoshiki, 813

Tu, C. M., 479Turner, W., 867

Udagawa, Shun-Ichi, 613Umeda, Makoto, 613

van Bijsterveld, 0. P., 278Vanderheiden, G. J., 875van Natta, J. P., 220, 394Vennes, J. W., 988Vermeire, Daniel, 696Verrall, M. S., 718von Graevenitz, Alexander, 176

Walker, Robert L., 245Wallace, R., 1025Wallnofer, P. R., 714Warren, J. A., 39Washington, J. A., It, 259, 589,

702Watkins, Harry M. S., 87Watrach, A. M., 932Weiss, K. F., 911Wells, Joy G., 401, 795Wenner, Herbert A., 791Westbrook, Louise, 53, 950White, Lloyd A., 701Wilcox, Clare, 629Wilfert, James N., 345Winely, C. L., 214Winkler, K. C., 278Wood, Ronald M., 618Woolley, Carolyn, 538Wright, D. N., 232, 865Wright, Herbert S., 92, 96Wu, B. J., 394Wynne, E. Staten, 731

Yall, Irving, 973Yang, H., 271Yates, S. G., 103Yesair, David W., 295Yin, E. Jong, 573Yotis, William W., 113, 474Yu, Pauline K. W., 259, 589, 702

Zacharia, Theodore P., 71Zimmerman, W. Douglas, 389Zwadyk, P., Jr., 746Zygmunt, W. A., 746

VOL. 19, 1970 * *

APPLIED MICROBIOLOGY, June 1970, p. iv-xviiCopynight © 1970 American Society for Microbiology

SUBJECT INDEX

VOLUME 19

Absorption of agglutinins from sera-influence on rubella hemagglutination-inhibition

test, 491Acetanilide-hydrolyzed by an F. solani acylamidase, 307Acid precipitation-for purification of M protein, 5Actinomycetales-motile nocardoid strains, 527Activated sludge-aerobic heterotrophic bacterial populations, 663,

671-utilization of aromatic compounds, 663Acylamidase-isolated from F. solani, 307Adventitious agents-detection in monkey kidney cell suspensions, 170Adventitious contamination-of primary bovine embryonic kidney cell cultures,

381Aeration-influence on formation of amylase, 535Aerobacter aerogenes-detection by labeling with 32p, 701Aerobic agitation-effect on growth of group N streptococci, 608Aerobic bacteria-automatic inoculator for, 1023-hydrolysis of conjugated bile acids by, 295Aeromonads-from man and other animals, 618Aerosol administration-pulmonary absorption and retention of kanamycin

after, 583Aerosol survival-of dry-disseminated E. coli B, 604Aerosolization-effect on survival of streptococcal L-forms, 865Aflatoxigenic isolates-of A.flavus from pecans, 128Aflatoxin-production in corn, 781-production with pecans as substrate, 128Agar dish isopiestic equilibration method-for controlling water potential of substrates, 536Agar-gel diffusion technique-arbovirus identification by, 557Agglutinating antisera-against P. tularensis, 894Agglutinins-for identification of medically important Candida

species, 830-in chickens infected with JM leukosis virus, 71Air purge-for monitoring oxygen in shake-flask fermentations,

551Airborne bacteria-influence of temperature on survival, 245

Airborne microbial survival-effect of changes in relative humidity, 232Airborne stability-of tailless bacterial viruses, 624Aldrin-degradation by soil microorganisms, 879Algae-oxygen production by, 76Alkaline substances-produced by spoilage flora in fresh beef, 902Alkylxylylene-bis-isothiuronium chlorides-antifungal activity of, 392Alpha toxin-effect of diethylstilbestrol on cytopathic action, 113Amino acid consumption-by B. pertussis, 512w-Amino acids-effect on staphylococcal infections, 813Ammonia content-for determining spoilage of crab cake mix, 734Amphotericin B-serum concentrations during therapy, 955Ampicillin-compared with cephalexin and penicillin V, 943-efficacy in shigellosis, 776Amylase-influence of pH and aeration on formation, 535-production by a strain of A. oryzae, 598Anaerobic bacteria-automatic inoculator for, 1023-in feces of patients receiving cobalt radiation ther-

apy, 458-recovery by use of prereduced media, 207Anaplasma marginale-preservation by liquid nitrogen storage, 700Animal viruses-propagation in poikilothermic cell line, 1

Animals-identification of aeromonads from, 618Antibacterial agent-isolated from the avocado pear, 470Antibacterial agents-effect of untreated whole milk, 220Antibiotic sensitivity testing-standardization of cultures for, 1015Antifungal antibiotic-produced by A. candidus, 718Antigen-influence on rubella hemagglutination-inhibition

test, 491Antimicrobial activity-instability effects in diffusion analyses of, 394Antimicrobial agents-microbioassay of, 573Antistaphylococcic agents-tested by prophylactic-therapeutic procedure, 813Antistreptolysin 0-automated microtitration test for, 621

iv

Vol. 19, No. 6Prillted in U.S.A.

SUBJECT INDEX

Antistreptolysin 0 hemolytic test-compared with antistreptolysin 0 latex screening

test, 60Antistreptolysin 0 latex screening test-compared with the antistreptolysin 0 hemolytic

test, 60Arbovirus-identification by agar-gel diffusion technique, 557Aromatic compounds-utilization by activated sludges, 663L-Asparaginase-production by S. marcescens, 800Aspergillus canididus-production of chlorflavonin by, 718Aspergillus flavus-aflatoxigenic isolates from pecans, 128-production of aflatoxin in corn, 781Aspergillus ochraceus-mycotoxicity to chicks, 594Aspergillus oryzae-production of amylase by, 598Aspergillus species-production of ochratoxin A by, 542Autoagglutination-inhibited in M. bovis by 10% MgCl2, 201Automated fluorescent treponemal antibody test-instrument and evaluation, 898Automated microtitration test-compared with tube-dilution and manual tech-

niques, 621-for antistreptolysin 0, 621Automatic inoculator-for bacterial cultures, 1023Avian tissue cultures-study of laryngotracheitis virus in, 932Avocado pear-new antibacterial agent isolated from, 470Azaphenanthrene compounds-antibacterial effect, 1017

Bacillus 29-induction of an MnO2-reductase system in, 966Bacillus popilliae-characteristics of a new strain, 681Bacillus sphaericus-degradation of urea herbicides by, 714Bacillus stearothermophilus spores-heat resistance at intermediate water activity, 565Bacillus subtilis var. niger spores-effect of ethylene oxide sterilization, 146-effect of temperature on airborne survival, 245Bacteria-detection in monkey kidney cell suspensions, 170Bacterial counts-for determining spoilage of crab cake mix, 734Bacterial cultures-automatic inoculator for, 1023Bacterial infection-resistance to induced by statolon and pyran, 188Bacterial populations-of sewage and activated sludge, 663, 671Bacterial spores-chemical states of, 565Bacterin-as vaccine against fowl cholera in turkeys, 837

Bacteriophage m13-protection of S. laciis ML3 from lytic activity of,

638Bacterium "lb"-description of, 176Bacteriuria-disposable kit for detection, 409Bacteroidaceae-dehydrogenase patterns in the study of, 505Bacteroides-identified by enzyme patterns, 505Bay 37289-effect on microbial activities in soil, 479Baygon-degradation by soil microorganisms, 879Beef-assessment of bacterial spoilage, 902Beer-enzymatic removal of diacetyl from, 649, 883Bile-agar-typical appearance of T. glabrata on, 184Biological exploration-of Mars, 32Bivalent antiserum-produced by P. tularensis-B. abortus vaccination,250

Black-pigmented strain-of M. fortuitum Cruz, 862Blood culture systems-evaluation of, 281Body fluids-assay of carbenicillin in, 264Bologna-behavior of salmonellae in, 190Bordetella pertussis-growth in medium with glutamic acid, 512Boston City Hospital-S. marcescens strains isolated at, 345Bovine adenovirus type 2-contaminant of primary bovine embryonic kidney

cell cultures, 381Bovine embryonic kidney cells-for titration of rinderpest virus, 545Brewer-jar techniques-compared with prereduced media, 207Broad-spectrum illumination source-bactericidal activity of, 1013Brucella abortus-combined vaccination with P. tularensis, 250Brucella ovis infection-method for testing sheep sera for, 335Burros-preparation of P. tularensis antisera in, 894Butschlia parva Schuberg-occurrence in the ovine rumen, 179

California arbovirus encephalitis-hemagglutination-inhibition test for, 398Candida-identification of medically important species, 830Candida albicans-cross-agglutination reaction in, 44Canned cured meats-effect of sodium nitrite on growth from spores, 353

VOL. 19, 1970 v

SUBJECT INDEX

Canned foods-spoilage bacteria in, 283Canned mushrooms-sulfide spoilage bacteria in, 283Capillary tubes-apparatus for transfer from hot to cold bath, 696Carbenicillin-assayed in body fluids, 264Carbon dioxide incubators-device for continuity of air supply, 174Cedar shingles-soft rot of, 872Cell association-of cottontail rabbit herpesvirus in vitro, 549Cell culture apparatus-adapted to a roller system, 541Cell culture systems-as substrates for influenza virus assay, 580Cell-free extracts-hydrolysis of conjugated bile acids by, 295Cell monolayers-assay of chikungunya virus in, 224Cell stability-of miniature cell culture systems, 87Cell temperature studies-use of a temperature-gradient incubator, 848Cell-to-cell transmission-of chikungunya virus infection, 389Cellophane-over-agar technique-for testing staphylococci for enterotoxigenicity, 698Cellulose membrane filters-poliovirus permeability of, 317Cephalexin-compared with penicillin V and ampicillin, 943-regression curves of, 589Cephaloglycin-regression curves for, 589Cephaloridine-regression curves for, 589Cephalosporin activity-regression curve analysis of, 589Cheese manufacture-use of frozen starter cultures, 928Cheese-starter cultures-effects of freezing and thawing, 928Chemical additives-effect on dengue virus plaque development, 757Chicken embryo tracheal organ cultures-large-quantity production of, 658Chickens-infected with JM leukosis virus, 71-Salmonella contamination of, 795Chicks-differential leukocyte counts of, 726-mycotoxicity of A. ochraceus to, 594Chikungunya virus

-atssayed by immunofluorescence, 224Chikungunya virus infection-cell-to-cell and extracellular transmission, 389Chikungunya virus vaccine-prepared by Tween-ether extraction, 321Chloramphenicol-effect on host-bacteriophage relationships, 707

Chliorella sorokiniania-effects of helium atmospheres on growth, 182Chlorflavonin-production by A. canididus, 718Chocolate-agar-typical appearance of T. glabrata on, 184Cholera antitoxin-titration in human sera, 742Cholera vibrios-streptomycin-dependent phenotypes, 463Chromatium viniosumn-characterization of, 988Ciliate protozoan-occurrence in the ovine rumen, 179Citrus fruit oils-antimicrobial action on food-borne bacteria, 27Cladosporium cladosporioides-disfigurement of paper by, 872Claviceps-genetically uniform strains of, 1005-nuclear distribution in the mycelium of, 1005Clinical isolation method-for C. vaginale, 47Clinical material-identification of staphylococci from, 919Clinical pharmacological studies-use of microbioassay of antimicrobial agents, 573Clostridia-production of hydrocinnamic acid, 375Clostridiumn botulitum types A, B, and E-differentiation by pyrolysis-gas-liquid chromatog-

raphy, 138Clostridium nigrificalls-medium for enumeration, 283Clostriditim perfringens-influence of water activity on growth, 980-inhibition of growth from spores by sodium nitrite,

353-pathogenicity for germ-free guinea pigs, 314-tested with incubation-type indole strip, 547C02-02 tensions-effects on growth of fungi from flue-cured tobacco,

27114 C02 assimilation-measurement in soils, 32Coagulase-mannitol-agar method-for identification of staphylococci, 919Coagulase-mannitol test strip-for speciation of staphylococci, 702Coagulase-positive staphylococci-polymyxin-coagulase-mannitol-agar for isolation,

73Cobalt radiation therapy-anaerobe levels in feces of patients before and after,

458-Sphaerophorus levels in feces of patients before and

after, 458Cohen and Wheeler broth-growth of B. pertussis in, 512Colistin-efficacy in shigellosis, 776Colorimetric assay-for lysine decarboxylase in E. coli, 367

Vi APPL. MICROBIOL.

SUBJECT INDEX

Column elution-for purification of M protein, 5Combination medium-for identification of Serratia and Providencia, 385Commercial agars-effect on dengue virus plaque development, 757Confidence interval method-for analyzing microbial populations of activated

sludges, 671Conjugated bile acids-hydrolysis by cell-free extracts, 295Contact method-for studying fowl cholera vaccines in turkeys, 842Continuous-particle electrophoresis-for separation of T. pallidum from tissue debris,

287Corn-aflatoxin production in, 781Corynebacterium vaginale-method for isolation and identification, 47Cottage cheese-prepared with concentrates of L. citrovorum, 890Cottontail rabbit herpesvirus-cell-associated nature in vitro, 549Coumermycin A1-susceptibility of N. meningitidis to, 329Coumermycins-structure-activity relationships, 14Coxiella burnetli-plaque assay for, 963Crab cake mix-bacteriological characteristics, 734Cross-agglutination reaction-in C. albicans and enteric bacilli, 44Culture concentrates-use in the manufacture of cheese, 890Cultures-standardized for antibiotic sensitivity testing, 1015Cutaneous bacteria-enumerated on human skin, 254-nitrogen requirements and uricolytic activity, 643CV-1 cell line-for measurement of rabbit pox virus, 791Cytoplasmic inclusion-studied by freeze-etching technique, 997

DDT-degradation by soil microorganisms, 879Death rate kinetics-of spore- and nonsporeforming bacteria exposed

to ethylene oxide, 152Dechlorogriseofulvin-detection by thin-layer and gas-liquid chroma-

tography, 106Defaunated sheep-effect of increased rumen ammonia-N levels, 485Dehydrogenase patterns-in the study of Bacteroidaceae, 505Dengue virus-variables influencing plaquing of, 751, 757Dengue virus plaque development-in simian cell systems, 751, 757Deoxyribonuclease test-for identification of staphylococci, 919

Deoxyribonucleic acid viral agent-in blood from patients with hepatitis, 521Depsipeptide antibiotics-production of, 109Dermatophytic infections-effect of haloprogin, 746Detergent-disinfectant combinations-effect on poliovirus and vaccinia virus, 53Diabetes-fluorescent treponemal antibody reactions in, 425Diacetyl-enzymatic removal from beer, 649, 883-produced by concentrates of L. citrovorum, 890Diacetyl reductase-for removal of diacetyl from beer, 649, 883-stabilization in beer, 883Diazinon-effect on microbial activities in soil, 4792,4-Dichlorophenoxyacetic acid-microbial content of soils treated with, 379Diethylaminoethyl-dextran-effect on laryngotracheitis virus, 932Diethylaminoethyl-Sephadex-for removing nonspecific inhibitors from sera, 785Diethylstilbestrol-action on staphylococci, 474-interaction with staphylococcal alpha toxin, 113Diffusion analyses-instability effects in, 394Diffusion-in-gel method-for diagnosis of ram epididymitis, 335Diluents-influence on rubella hemagglutination-inhibition

test, 491Dip-inoculum method-with disposable kit for urine cultures, 409Disinfectants-viricidal activity against vesicular stomatitis virus,

92, 96Disposable method-for quantitative cultures of urine, 409Dissolved oxygen levels-effect on production of L-asparaginase and pro-

digiosin, 800Dodecylxylylene-bis-isothiuronium chloride-antifungal activity of, 392Dry contact of fabrics-virus transmission by, 950Dry-disseminated aerosols-survival of, 604Dubos oleic acid agar medium-for differentiating meningococci from gonococci,

1025Duckweed-for measuring environmental stress, 1009Dursban-effect on microbial activities in soil, 479

Eastern-type turkey rolls-bacteriological examination, 768Ecosystem response-to a sustained stress, 1009Eijkman fecal coliform concept-formic hydrogenlyase induction as a basis for, 441

*iiVOL. 19, 1970

SUBJECT INDEX

Electrical current-lethal effects on E. coli, 421Electron microscopic count-of virus particles in smallpox vaccines, 689Embryonating chicken eggs-for evaluating disinfectants as viricides, 92Endrin-degradation by soil microorganisms, 879Energy of activation-of thermal inactivation of Newcastle disease virus,

451Enteric bacilli-cross-agglutination reaction in, 44Enteric viruses-ultraviolet devitalization of, 805Enterobacter-effect of incubation at 44.5 C on gas production,

441-method for differentiation from Serratia, 134-test for differentiation from Klebsiella, 438-two biochemical types in aquatic environment, 441Eniterobacteriaceae-cross-agglutination reaction in, 44-deoxyribonuclease activity of, 385-phenylalanine deamination by, 385-tested with incubation-type indole strip, 547Enterococcus-activity of antibiotics against, 629Enterotoxins-high titers for the testing of staphylococci, 698Enteroviruses-isolation from sewage, 120Entropy of activation-of thermal inactivation of Newcastle disease virus,

451Environmental stress-method for measuring, 1009Enzyme patterns-for differentiation of leptospirae, 586Epidemiological studies-of the fish meal industry, 401Epornitic method-for studying fowl cholera vaccines in turkeys, 842Erythrocytes-influence on rubella hemagglutination-inhibition

test, 491Erythromycin estolate---serum antibacterial activity after administration of,

370Escherichia coli-accumulation by the quahaug, 239-assay for lysine decarboxylase in, 367-effect of temperature on airborne survival, 245-lethal effects of electrical current, 421Escherichia coli B-survival of dry-disseminated aerosol, 604Escherichia coli-Shigellaflexneri hybrid-in intestinal tract of Macaca mulatta, 731Essential oils-antimicrobial properties, 27Esterase activity-localization in L. casei, 1001Estuarine water-ultraviolet devitalization of viruses in,'805

Ether-resistant enteroviruses-isolation from sewage, 120Ethylene oxide-influence of thickness of polyethylene film on

activity, 163-microbial resistance to, 152Ethylene oxide sterilization-microbiological aspects, 146, 152, 157, 163Euphotic zone-microbial biomass on organic matter in seawater of,960

Extracellular transmission-of chikungunya virus infection, 389

Fabrics-as disseminators of virus, 950Fathead minnow poikilothermic cell line-viral susceptibility range, 1Faunated sheep-effect of increased rumen ammonia-N levels, 485Fecal specimens-media for isolation of shigellae from, 434Fescue pasture-relation to toxicity of Fusarium isolates, 103Field isolation of viruses-use of miniature cell culture systems, 87Fish meal contamination-method of control, 401Fish meal plants-salmonellae in, 401"Fitness for human consumption" factor-use in evaluating fowl cholera vaccines, 844Flow chart-for safety testing monkey kidney cell suspensions,

170Flue-cured tobacco-growth of fungi isolated from, 271Fluorescent-antibody conjugates-against P. tularensis, 894Fluorescent-antibody stain technique-with group A streptococci, 940Fluorescent-antibody test-for smallpox, 855Fluorescent treponemal antibody-absorption test-compared with automated fluorescent treponemal

antibody test, 898Fluorescent treponemal antibody reactions-in diabetes, 425Fluorescent treponemal antibody tests-evaluation of, 425Fluorimetric method-for monitoring nicotinamide nucleotides in con-

tinuous cultures, 446Food-borne bacteria-antimicrobial action of citrus fruit oils, 27Foodstuffs-isolation of toxin-producing fungus from, 613Formaldehyde gas-quantitated in air, 1021Formalin-effect on influenza virus, 290Formalin-inactivated vaccine-compared with vaccine prepared by Tween-ethermethod, 321

. .i. APPL. MICROBIOL.

SUBJECT INDEX

Formalinized erythrocytes-for titration of cholera antitoxin, 742Formamide hydrolysis-for extracting Streptococcus group E antigens, 818Formic hydrogenlyase induction-as a basis for the Eijkman fecal coliform concept.

441Fowl cholera immunization-in turkeys, 837, 842, 844Freeze-etching-for analysis of reovirus-infected cells, 997Freeze-injured cells-pathogenicity of, 39French pressure cell-laboratory press used with, 875Frozen concentrated starter cultures-use in cheese manufacture, 928Frozen food-collection of samples from, 878Fungal diseases-treated with amphotericin B, 955Fungal disfigurement-of paper, 872Fungi-effects of N2-02 and C02-02 tensions on growth,

271-growing on tall fescue grass, 103-inhibition by alkylxylylene-bis-isothiuronium chlo-

rides, 392-toxic properties, 613-utilization of prometryne by soil isolates, 11Fungus development-environmental conditions for, 1007Funnel-for collection of samples from frozen food, 878Fusarium isolates-correlation of toxicity to toxins in fescue pasture,

103Fusarium solani-an acylamidase of, 307-herbicide utilization by, 301, 307Fusobacterium-identified by enzyme patterns, 505

Gamma isomers of benzenehexachloride-degradation by soil microorganisms, 879Gamma radiation-for inactivation of Venezuelan equine encephalitis

vaccines, 763Gas-liquid chromatography-for detection of griseofulvin and dechlorogriseo-

fulvin, 106Genetically uniform strains-of Claviceps, 1005Genital mycoplasmas-susceptibility to antimicrobial agents, 62Genitourinary tract-recovery of Mycoplasma from, 268Germ-free guinea pigs-pathogenicity of C. perfringens for, 314Germicide-treated fabrics-evaluation of zone of inhibition, 193Glucose-effect on growth of C. perfringens, 980

Glutamic acid-in medium for cultivation of B. pertussis, 512Gonococci-differentiated from meningococci, 1025Gram-negative bacilli-activity of minocycline against, 259Gram-negative bacteria-effect of compounds from avocado, 470-effect of diethylstilbestrol, 474-survival on plastic containing hexachlorophene, 131Gram-negative rod-description of, 176Gram-positive bacteria-activity of minocycline against, 259-effect of compounds from avocado, 470-effect of diethylstilbestrol, 474Griseofulvin-detection by thin-layer and gas-liquid chromatog-

raphy, 106Group A streptococci-fluorescent-antibody stain technique with, 940-M protein preparations of, 5Group antigen-separated from type antigen of Streptococcus group

E, 823Group D Streptococcus-activity of antibiotics against, 629Group N streptococci-hydrogen peroxide production by, 608Growth medium-effect on elution patterns after pyrolysis, 138Guinea pigs-pathogenicity of C. perfringens for germ-free and

conventional types, 314

Haemophilus vaginalis-method for isolation and identification, 47Haloprogin-activity compared with tolnaftate, 746-topical antifungal agent, 746Heat resistance-apparatus for determination in spores, 696-of bacterial spores at intermediate water activity,

565-of salmonellae, effect of water activity, 429Helium atmospheres-effect on growth of C. sorokiniana, 182Hemagglutination-inhibition test-for California arbovirus encephalitis, 398Hematopoietic cell line media-substitution of serum by synthetic polymers, 906Hepatitis-viral agent in blood from patients with, 521Herbicide transformation-by F. solani, 301, 307Herpesviruses-classification of, 549Hexachlorophene-surface action against gram-negative organisms,

131Histoplasma capsulatum-maintenance of the mycelial phase, 413Homoiothermic viruses-susceptibility of poikilothermic cell line to, 1

VOL. 1 9, 1970 iX

SUBJECT INDEX

Horses-preparation of P. tularensis antisera in, 894Host-bacteriophage relationships-effect of chloramphenicol, 707Human diploid cell strain WI-38-growth of Venezuelan equine encephalomyelitis

virus in, 196Human sera-titration of cholera antitoxin in, 742Human skin-microbial flora of, 254Hydraulic press-used with French pressure cell, 875Hydrochloric acid-for extracting Streptococcus group E antigens, 818Hydrocinnamic acid-production by clostridia, 375Hydrogen peroxide-effect on growth of group N streptococci, 608-production by group N streptococci, 608Hydrogen sulfide-produced by bacteria in meat, 937Hydrolase-for hydrolysis of conjugated bile acids, 295Hydroxylamine-M. kansasii identified by susceptibility to, 199

Immunofluorescence-for assay of chikungunya virus, 224-for smallpox diagnosis, 855Incinerator stack emissions-portable sampler for microorganisms in, 204Incubation-type indole strip test-evaluation of, 547Inert gases-effects on growth of C. sorokiniana, 182Infants-listeric lesions compared with those in mice, 772Infectious agents-associated with shearling bedpads, 53Influenza virus-effects of various inactivation methods, 290Influenza virus assay-cell culture systems as substrates, 580Inoculum size-effect on susceptibility of streptococci to anti-

biotics, 629Instability effects-in diffusion analyses of antimicrobial activity, 394Interferon production-enhanced by poly-D-lysine, 867Intermediate water activity-bacterial spore heat resistance at, 565Intraperitoneal injections-errors in placement in mice, 704Ionizing radiation-in preparation of Venezuelan equine encephalitis

vaccines, 763Irradiation-recycled Salmonella-Dublic health significance, 338Isohumulone-antibiotic properties, 871

JM leukosis virus-agglutinins in chickens infected with, 71

Kanamycin-efficacy in shigellosis, 776-pulmonary absorption and retention, 583Kaolin-serum treatment-for California arbovirus encephalitis, 398KCI-effect on growth of C. perfringens, 980KH2PO4-effect on phosphohexosan production by yeasts,

1019Klebsiella-test for differentiation from Enterobacter, 438

Laboratory identification-of aeromonads from man and other animals, 618Laboratory press-for use with French pressure cell, 875Lactic streptococcal bacteriophage-effect of spermine on, 638Lactic streptococci-effect of chloramphenicol on host-bacteriophage

relationships, 707-effect of freezing and storage in nitrogen, 1025Lactobacillus casei-localization of esterase activity, 1001Large intestine of man-unusual Sphaerophorus species from, 458Laryngotracheitis virus-effect of diethylaminoethyl-dextran on infectivity,932

Lemna minor-for measuring environmental stress, 1009Leptospira-differentiation of pathogens and nonpathogens,

586-naphthylamidase activity of, 586Leuconostoc citrovorum-concentrated cultures of, 890Leukocyte counts-in chicks inoculated with Salmonella, 726Lid method-for evaluating zone of inhibition of treated fabrics,

193Light intensity-effect on oxygen production by algae, 76Lipophilic bacteria-enumerated on human skin, 254Liquid medium-induced sporulation of B. popillae in, 681Liquid nitrogen repository-preservation of A. marginale in, 700Liquoid-in blood culture systems with or without sucrose,

281Listeria monocytogenes-histopathology after oral feeding to mice, 772Liver cheese-behavior of salmonellae in, 190Low-frequency alternating current-effect on viability of E. coli, 421Lucheon meat-behavior of salmonellae in, 190Lupulone-antibiotic properties, 871

APPL. MICROBIOL.x

SUBJECT INDEX

Lysine decarboxylase-assayed in E. coli, 367

M protein-acid-precipitated and column-eluted preparations, 5-preparations from group A streptococci, 5Macaca mulatta-persistence of E. coli-S. flexneri hybrid in, 731MacConkey agar-for isolating shigellae from fecal specimens, 434-typical appearance of T. glabrata on, 184Magnesium chloride-inhibitor of autoagglutination of M. bovis, 201Man-identification of aeromonads from, 618Manganese nodules-bacteriology of, 966Marine bacillus-induction of an MnO2-reductase system in, 966Market quality-use in evaluating fowl cholera vaccines, 844Mars-experiment for biological exploration, 32MDCK-USD canine kidney cell cultures-for influenza virus assay, 580Meat-formation of sulfmyoglobin in, 937Medium-effect on production of amylase by A. oryzae, 598Membrane filters-passage of T. pallidwn through, 326Meningococci-differentiated from gonococci, 1025Merthiolate-effect on influenza virus, 290Metabolic injury-of S. gallinarum after freezing, 39Metabolite fluorimeter-for monitoring nicotinamide nucleotides in con-

tinuous cultures, 446Methylpyrrole-noviose-coumarin amides-structure-activity relationships, 14Methylthio-s-triazine-utilization for growth of soil fungi, 11Mice-histopathology after ingestion of L. monocytogenes,

772-infected with S. flexneri for chemotherapy studies,

776-techniques of intraperitoneal injection, 704Microbial biomass-on particulate organic matter in Seawater, 960Microbial cells-determined in incinerator stack emissions, 204Microbioassay-of antimicrobial agents, 573Microbiological assay-for (-thioguanine deoxyriboside in tissues of mice,

538Micrococcaceae-identified with coagulase-mannitol test strip, 702Microtiter hemagglutination tests-for titration of cholera antitoxin, 742

Microtitration-with automatic serial-dilution machine, 621Mildew testing-viability control for, 1007Milk-effect on antibacterial activity of compounds, 220Milk chocolate-heat resistance of S. anatum in, 177Milk products-use of citrus oils to increase shelf life, 27Miniature cell culture systems-cell stability and sensitivity to virus infection, 87-use in field isolation of viruses, 87Minimal vitamin medium-for maintaining mycelial phase of H. capsulatum,

413Minocycline-in vitro antibacterial activity, 259MnO2-reductase system-induction in a marine bacillus, 966Moisture-effect on heat resistance of S. anatum in chocolate,

177-effect on aflatoxin production in corn, 781Monamycins-production of, 109Monilial infections-effect of haloprogin, 746Monkey kidney cell suspensions-flow chart for safety testing, 170Monkeys-persistence of E. coli-S. flexneri hybrid in, 731-streptococcal infections in, 943Moraxella bovis-autoagglutination inhibited by 10% MgC92, 201Moraxella nonliquefaciens-selective medium for, 278Morphological procedures-for identification of Candida species, 830Motile nocardoid bacteria-identification of, 527Mountford's index-for analyzing microbial populations of activated

sludges, 671MS-2 bacterial virus-airborne stability of, 624Mucous membranes-growth of Moraxella on, 278Mueller Hinton agar-antibacterial activity of minocycline in, 259-typical appearance of T. glabrata on, 184Mumps antibody-method of assay, 360Mumps virus-plaque formation by, 360Mycelial phase-maintenance in H. capsulatum, 413Mycobacteria-effect of UV on paper contaminated with, 383Mycobacterium fortuitum Cruz-black-pigmented strain from, 862Mycobacterium kansasti-identified by susceptibility to hydroxylamine, 199

VOL. 19, 1970 Xi

SUBJECT INDEX

Mycoplasma-recovery from male genitourinary tract, 268Mycoplasma fermentans-susceptibility to antimicrobial agents, 62Mycoplasma hominis-susceptibility to antimicrobial agents, 62Mycoplasma pneumoniae-response to changes in relative humidity, 232Mycoplasma studies-use of chicken embryo tracheal organ cultures,

658Mycoplasmas-human genital strains, 62Mycoses-treated with amphotericin B, 955Mycotic infections-effect of haloprogin, 746

N'-methoxy phenylurea herbicides-degradation by B. sphaericus, 714N2-02 tensions-effects on growth of fungi from flue-cured tobacco,

271NaCl-effect on growth of C. perfringens, 980Naphthylamidase activity-of Leptospira, 586Neisseria gonorrhoeae-differentiated from N. meningitidis, 1025Neisseria meningididis-differentiated from N. gonorrhoeae, 1025-susceptibility to antimicrobial agents, 329Neomycin-efficacy in shigellosis, 776Neutral red-effect of dengue virus plaque development, 751Newcastle disease virus-effect of temperature on radiosensitivity, 455-radiation inactivation of, 455-thermal inactivation of, 451Nitrite oxidation-effects of pesticides in N. agilis, 214Nitrobacter agilis-effects of pesticides on nitrite oxidation, 214Nitrogen-nutrition requirements for skin bacteria, 643Nocardoid strains-of Actinomycetales, 527Nonlipophilic bacteria-enumerated on human skin, 254Nonspecific inhibitors-method for removal from sera, 785Nonsporeforming bacteria-resistance to ethylene oxide, 146, 152Nuclear distribution-in the mycelium of Claviceps, 1005Nucleoside phosphotransferase production-for differentiation of Serratia from Enterobacter,

134Nucleotides-separation by thin-layer chromatography, 134Nutrient agar overlay-effect on dengue virus plaque development, 751

Nutritional requirements-of P. flabellatus, 166

Ochratoxin A-effects on chicks, 594-produced by Aspergillus species, 542Oerskovia gen. n.-new genus of nocardoid bacteria, 527-harboring 0rskov's motile Nocardia, 534Oerskovia turbata comb. n.-type species of Oerskovia gen. n., 534Off-flavor in beer-eliminated with diacetyl reductase, 883Orange oil-antimicrobial properties, 27Organophosphorus insecticides-effect on microbial activities in soil, 4790rskov's motile Nocardia-harbored by Oerskovia gen. n., 534Ovine rumen-occurrence of B. parva Schuberg in, 179Oxidation-prevented in transport media, 870Oxygen concentrations-continuous monitoring in shake-flask f

tions, 551Oxygen probes-for monitoring oxygen in shake-flask f

tions, 551Oxygen production-by algae, 76Oxytetracycline-efficacy in shigellosis, 776

rermenta-

rermenta-

32p-detection of A. aerogenes by labeling with, 701Paper-effect of UV on contamination with mycobacteria,

383-fungal disfigurement of, 872Parallel air supply-for CO2 incubators for tissue culture, 174Partial purification-of smallpox vaccines, 689Particulate organic matter-in seawater of the euphotic zone, 960Pasteurella multocida cell fractions-as vaccines against fowl cholera in turkeys, 837Pasteurella pestis-presumptive identification and isolation, 915Pasteurella tularensis-combined vaccination with B. abortus, 250Pasteurella tularensis antisera-preparation in burros and horses, 894Pasteurella tularensis-Brucella abortus vaccination-agglutinin response of rabbits to, 250Peanuts-mycotoxicity of A. ochracelus isolates from, 594Pecans-aflatoxigenic isolates of A.flavus from, 128-as a substrate for aflatoxin production, 128Pediatric research-use of microbioassay of antimicrobial agents, 573

*xii APPL. MICROBIOL.

SUBJECT INDEX

Penicillin V-compared with cephalexin and ampicillin, 943Penicillium purpurogenum Stoll-production of rubratoxin B by, 613Penicillium urticae-griseofulvin and dechlorogriseofulvin extracted

from, 106Per cent sporulation-effect on elution patterns after pyrolysis, 138Pesticides-effects on nitrite oxidation by N. agilis, 214Pesticinogeny-for identification of P. pestis, 915pH-effect on dengue virus plaque development, 751-effect on production of amylase by A. oryzae, 598-influence on formation of amylase, 535pH of medium-effect on susceptibility of streptococci to antibiotics,

629Phenanthrene-position of aza, methyl, and benzyl groups related

to antibacterial activity, 1017Phosphohexosans-composition in yeasts, 1019Phosphorus-removal from wastewaters by sludge, 973Photochromogen test-hydroxylamine susceptibility as an auxiliary test,

199Plague-use of pesticin assay in study of, 915Plaque assay-for Q fever and scrub typhus rickettsiae, 963Plaque formation-by various mumps virus strains, 360Plaque reduction neutralization procedure-for assay of mumps antibody, 360Plastic compounded with hexachlorophene-survival of gram-negative bacteria on, 131Pleurotis flabellatus-as a food source, 166-nutritional requirements of, 166Poliovirus-disseminated by fabrics, 950-effect of detergent-disinfectant combinations, 53Poliovirus type 1-devitalization compared with other viruses, 805Pollution-elimination by use of electric current, 421Poly-D-lysine-effect on interferon induction in mice, 867Poly L:C-interferon induction in mice by, 867Polycarbonate filters-poliovirus permeability of, 317Polyethylene film-effect of thickness on activity of ethylene oxide,

163Polymers-substituted for serum in hematopoietic cell line

media, 906

Polymyxin-coagulase-mannitol-agar-for isolation of coagulase-positive staphylococci,

73Polyurethane foam cylinders-for isolation of soil microorganisms, 186Population structure-of bacteria of activated sludge, 671Pore size-effect on passage of T. pallidum through membrane

filters, 326-effect on poliovirus permeability of filters, 317Portable sampler-for microorganisms in incinerator stack emissions,

204Poultry-processing plant-Salmonella contamination of, 795Precipitation reactions-of arboviruses, 557Precipitin-in-agar technique-for differentiation of arboviruses, 557Prereduced media-for recovery of anaerobic microorganisms, 207Primary bovine embryonic kidney cell cultures-type 2 bovine adenovirus as a contaminant, 381Primary hamster kidney cell cultures-for influenza virus assay, 580Primary rhesus monkey kidney cell cultures-for influenza virus assay, 580Prodigiosin-production by S. marcescens, 800Prometryne-utilization for growth of soil fungi, 11Propanil-transformation by F. solani, 301, 307Prophylactic-therapeutic procedure-for testing antistaphylococcic activity of compounds,

813,B-Propiolactone-effect on influenza virus, 290Providencia-medium for identification, 385Pseudomonas aeruginosa-use in assay for carbenicillin, 264Pseudomonas aureofaciens-metabolism of tryptophan, 721Pseudomonas mephitica-producer of H2S in meat, 937Pulmonary system-absorption and retention of kanamycin, 583Purified conjugate-use in fluorescent-antibody test for smallpox, 855Purified suckling rat brain rabies vaccine-preparation of, 923Purple sulfur bacteria-occurrence in a sewage treatment lagoon, 988Pyran-resistance to bacterial infection induced by, 188Pyrolysis-gas-liquid chromatography-for differentiation of C. botulinum types A, B, and

E, 138Pyrrolnitrin biosynthesis-effects of tryptophan analogues, 721

VOL. 19, 1970 *x*ii

SUBJECT INDEX

Q fever rickettsiae-plaque assay for, 963Quahaug-accumulation of E. coli by, 239

Rabbit pox-viremia and virus measurements, 791Rabies vaccine-prepared from suckling rat brain, 923-purified by zonal centrifugation, 923Radiation inactivation-of Newcastle disease virus, 455Radiation recycling-effect on Salmonella, 338Radioactive phosphorus-detection of A. aerogenes by labeling with, 701Ram epididymitis-diffusion-in-gel method for diagnosis, 335Reagent-impregnated coagulase-mannitol test strip-evaluated with Micrococcaceae, 702Reduced nicotinamide nucleotides-technique for monitoring changes in levels, of, 446Refrigerated conditions-for short-term storage of trypsinized tissues, 387Refrigerated storage-bacterial counts of turkey rolls during, 768Refrigerator spoilage-of fresh beef, 902Regression curve analysis-of cephalosporin activity, 589Relative humidity-effect of changes on M. pneumoniae, 232-effect on airborne stability of bacterial viruses, 624-effect on sporicidal activity of ethylene oxide, 157-effect on survival of streptococcal L-forms, 865Removal of nonspecific inhibitors from sera-influence on rubella hemagglutination-inhibition

test, 491Reovirus-infected cells-studied with freeze-etching technique, 997Replica-stamp technique-for isolation of soil microorganisms, 186Respiratory secretions-source of Serratia isolates, 345Rickettsia tsutsugamushi-plaque assay for, 963Rickettsiae-plaque assay for, 963Rifampin-susceptibility of N. meniingitidis to, 329Rinderpest virus-Vero cells for titration of, 545Roller bottles-accommodated in a roller-tube apparatus, 541Roller-tube apparatus-adapted to accommodate roller bottles, 541Rubella hemagglutination-inhibition test-effect of variables on antigen and antibody titers,

491Rubratoxin B-production by P. purpurogenum Stoll, 613Rumen ammonia levels-relation to microbial population in sheep, 485-relation to volatile fatty acid ratios in sheep, 485

S-13 bacterial virus-airborne stability of, 624Salmonella-characteristics of a new serotype, 694-effect of radiation recycling, 338-leukocyte counts in chicks inoculated with, 726-poultry-processing plant contaminated with, 795Salmonella anatum-heat resistance in milk chocolate, 177Salmonella anatum infections-effect on leukocyte counts of chicks, 726Salmonella enteritidis-serotype 501,2,3:Z4, Z24:-, 694-serotype Lovelace, 172Salmonella gallinarum-pathogenicity after metabolic injury, 39Salmonella heidelberg infections-effect on leukocyte counts of chicks, 726Salmonella subgenus IV-characteristics of a new serotype, 694Salmonellae-behavior in dry and semi-dry environments, 429-behavior in sliced luncheon meat, 190-effect of water activity on heat resistance, 429-in fish meal plants, 401Scrub typhus rickettsiae-plaque assay for, 963Seawater-microbial biomass on particulate matter in, 960Selective medium-for M. nonliquefaciens, 278Semisynthetic coumermycins-structure-activity relationships, 14Sensitivity to virus infection-of miinature cell culture systems, 87Sera-removal of nonspecific inhibitors, 785Serial-dilution machine-in automated microtitration test, 621Seroidentification-of medically important Canzdida species, 830Serological procedure-for detecting agglutinins in JM virus-infected

chickens, 71SeroMatic System-use in automated fluorescent treponemal antibody

test, 898Serratia-medium for identification, 385-method for differentiation from Entterobacter, 134Serratia marcescens-characteristics and antibiotic susceptibility, 345-effect of broad-spectrum illumination, 1013-effect of temperature on airborne survival, 245-production of L-asparaginase and prodigiosin, 800Serum-amphotericin B concentrations during therapy, 955-effect on dengue virus plaque development, 751-substituted by synthetic polymers in hematopoietic

cell line media, 906Serum antibacterial activity-after administration of triacetyloleandomycin and

erythromycin estolate, 370

XiV APPL. MICROBIOL.

SUBJECT INDEX

Serum plates-relationship between antiserum titer and zone

development, 911Sewage-aerobic heterotrophic bacterial populations, 663,

671-isolation of ether-resistant enteroviruses, 120Sewage treatment lagoon-occurrence of purple sulfur bacteria, 988Sewer-swab method-for isolation of viruses from sewage, 120Shake-flask fermentations-continuous monitoring of oxygen levels in, 551Shearling bedpads-infectious agents associated with, 53Sheep-effect of increased ammonia-N levels, 485Sheep blood-agar-typical appearance of T. glabrata on, 184Shellfish-parameters significant to accumulation of patho-

gens, 239Shigella flexneri-chemotherapy of mice infected with, 776Shigellae-media for isolation and transport, 434Short-term storage-of trypsinized tissues, 387Silver filters-poliovirus permeability of, 317Simian cell systems-dengue virus plaque development in, 751, 757Skin bacteria-nutrition of, 643Smallpox-direct fluorescent-antibody test for, 855Smallpox vaccines-virus particle content, 689Sodium bicarbonate-effect on dengue virus plaque development, 751Sodium nitrite-inhibitor of growth from spores of C. perfringens,

353Sodium sulfite method-for quantitating formaldehyde, 1021Soft rot-of cedar shingles, 872Soil fungi-utilization of methylthio-s-triazine for growth, 11Soil microbial activity-effect of organophosphorus insecticides, 479Soil microorganisms-degradation of chlorinated hydrocarbon insecti-

cides, 879-improved method for isolating, 186Soils-measurement of 14C02 assimilation, 32-microbial content after treatment with herbicides,

379Solid substrates-method for control of water potential, 536Somatic antigens-of Streptococcus groups E, 818, 823

Spermine-effect on lysis of S. lactis ML3, 638Sphaerophorus-from the large intestine of man, 458-identified by enzyme patterns, 505Sphaerotilus-growth in thermally polluted environment, 100Sporeforming bacteria-resistance to ethylene oxide, 146, 152Spores-apparatus for determining heat resistance, 696Sporulation-of a B. popillae strain in vitro, 681SS agar-for isolating shigellae from fecal specimens, 434Standard titers-determined for staphylococcal antisera, 911Staphylococcal alpha toxin-interaction with diethylstilbestrol, 113Staphylococcal antiserum titer-relation to zone development on serum plates, 911Staphylococcal infections-effect of w-amino acids, 813Staphylococcal membrane-role in action of diethylstilbestrol, 474Staphylococci-action of diethylstilbestrol, 474-high titers of enterotoxins for testing, 698-identified from clinical material, 919-isolation medium for coagulase-positive strains, 73-speciation with coagulase-mannitol test strip, 702Staphylococcus aureus-effect of broad-spectrum illumination, 1013Statolon-resistance to bacterial infection induced by, 188Streptococcal infections-comparison of cephalexin, penicillin V, and

ampicillin, 943Streptococcal L-forms-recovery at various relative humidities, 865Streptococcus-activity of antibiotics against, 629Streptococcus group E-somatic antigens of, 818, 823Streptococcus lactis ML3-effect of spermine on lysis by phage mi3, 638Streptolysin 0 reagents-in latex screening and hemolytic tests, 60Streptomyces jamaicensis-production of monamycins by, 109Streptomycin-dependent cholera vibrios-in vitro and in vivo studies of, 463-possible use as oral immunizing agents, 463Structure-activity relationships-of semisynthetic coumermycins, 14Suckling rat brain-rabies vaccine prepared from, 923Sucrose-in blood culture systems with or without Liquoid,

281Sulfide spoilage bacteria-in canned mushrooms, 283

VOL. 19, 1970 xv

SUBJECT INDEX

Sulfmyoglobin-formation in&meat,'937Suspending medium-effect on poliovirus permeability of filters, 317Synthetic medium-ochratoxin A produced by Aspergillus in, 542Synthetic polymers-substituted for serum in hematopoietic cell line

media, 906Syphilis-automated fluorescent treponemal antibody test for

diagnosis, 898

T-strain mycoplasmas-susceptibility to antimicrobial agents, 62Tailless bacterial viruses-airborne stability of, 624Temperature-effect on aflatoxin production in corn, 781-effect on radiosensitivity of Newcastle disease virus,

455-influence on survival of airborne bacteria, 245Temperature-gradient incubator-for cell and virus temperature studies, 848Ten-minute test-for differentiating between Klebsiella and Entero-

bacter, 438Terpineol-antimicrobial properties, 27Thermal inactivation-of Newcastle disease virus, 451Thermally polluted environment-growth rate of Sphaerotilus in, 100Thermochemical death-rate apparatus-for study of ethylene oxide sterilization, 146Thickness of culture solution-effect on oxygen production by algae, 76Thin-layer chromatography--for detection of griseofulvin and dechlorogriseo-

fulvin, 106-for separation of nucleotides, 134Tliiocapsa floridana-characterization of, 988Thioglycolate medium-compared with prereduced media, 207,B-Thioguanine deoxyriboside-assay and tissue distribution in mice, 538Thiolacetic acid-hydrolyzed by L. casei, 1001Tissue culture incubators-device for continuity of air supply, 174Tissue debris-separation of T. pallidum from, 287Tissue distribution-of 3-thioguanine deoxyriboside in mice, 538Titrimetric method-for assessing bacterial spoilage of beef, 902Tolnaftate-activity compared with haloprogin, 746Torulopsis glabrata-typical appearance on routine media, 184Toxins-produced by fungi growing on fescue grass, 103

Transport media-prevented from undergoing oxidation, 870Treponema pallidum-passage through membrane filters, 326-separation from tissue debris, 287Triacetyloleandomycin-serum antibacterial activity after administration of,

370Trichloroacetic acid-for extracting Streptococcus group E antigens, 818Trifluralin-microbial content of soils treated with, 3791, ,4-Trihydroxy-n-hepadeca-1 6-en-antibacterial activity of, 470Trimethylamine nitrogen content-for determining spoilage of crab cake mix, 734Tropical chambers-mildew testing in, 1007Trypsin treatment-of group A streptococci for fluorescent-antibody

staining, 940Trypsinized tissues-short-term storage at 4 C, 387Trypticase Soy Agar-antibacterial activity of minocycline in, 259Tryptophan-metabolism by P. aureofaciens, 721Tryptophan analogues-effects on pyrrolnitrin biosynthesis, 721Turbidity standardization-of cultures for antibiotic sensitivity testing, 1015Turkey rolls-bacteriological examination, 768Turkeys-fowl cholera immunization in, 837, 842, 844Tween-ether extraction-chikungunya virus vaccine prepared by, 321Type antigen-nonserological means of detection, 823-separated from group antigen of Streptococcius

group E, 823

Ultraviolet devitalization-of eight enteric viruses, 805Ultraviolet irradiation-effect on influenza virus, 290-effect on paper contaminated with mycobacteria,

383-for studying growth rate of Sphaerotiluis, 100Untreated whole milk-effects on antibacterial activity of compounds, 220Urea herbicides-degradation by B. sphlaericus, 714Urease paper-reagent strip-for differentiating between Klebsiella and Euitero-

bacter, 438Urethral swab-recovery of Mycoplasma from, 268Uricolytic activity-of cutaneous bacteria, 643Urine-method for quantitative cultures of, 409-recovery of Mycoplasma from, 268-source of Serratia isolates, 345

xvi APPL. MICROBIOL.

SUBJECT INDEX

Vaccinia virus-disseminated by fabrics, 950-effect of detergent-disinfectant combinations, 53Vapor concentration-quantitated for formaldehyde, 1021Venezuelan equine encephalitis vaccines-inactivated by gamma radiation, 763Venezuelan equine encephalomyelitis virus-growth in human diploid cell strain WI-38, 196Vero cells-for titration of rinderpest virus, 545Vesicular stomatitis virus-viricidal activity of disinfectants against, 92, 96Vibrio-streptomycin-dependent phenotypes, 463Viral agent-in blood from patients with hepatitis, 521Viral hemagglutination-inhibiting antibodies-extracted from nonspecific inhibitors of serum, 785Viral susceptibility range-of fathead minnow poikilothermic cell line, 1Viremia-of rabbit pox virus, 791Viricides-test method for evaluating, 92Virus inactivation-effect of various disinfectants, 96Virus particle content-of smallpox vaccines, 689Virus studies-use of chicken embryo tracheal organ cultures,

658Virus temperature studies-use of a temperature-gradient incubator, 848Viruses-disseminated by fabrics, 950-miniature cell cultures for field isolation, 87Volatile fatty acid proportions-relation to rumen ammonia levels in sheep, 485

Volatile reducing substances content-for determining spoilage of crab cake mix, 734Volutin granules-in Z. ramigera, 973

Wastewaters-removal of phosphorus from, 973Water activity-effect on sporicidal activity of ethylene oxide, 157-influence on growth of C. perfringens, 980-influence on heat resistance of salmonellae, 429Water content-effect on aerosol survival of bacteria, 604Water potential-method for control in solid substrates, 536WI-38 cells-growth of Venezuelan equine encephalomyelitis

virus in, 196

Xylose-lysine-deoxycholate agar-for isolating shigellae from fecal specimens, 434

Yeast extract-effect on sporulation of B. popillae, 681Yeast phase medium-for maintaining mycelial phase of H. capsulatum,

413Yeasts-composition of phosphohexosans, 1019

Zinophos-effect on microbial activities in soil, 479Zone development-relation to antiserum titer on serum plates, 911Zone of inhibition-evaluated in treated fabrics, 193Zoogloea ramigera-volutin granules in, 973

VOL. 19, 1970 x*ii

APPLIED

MICROBIOLOGY

VOLUME 19

BALTIMORE, MD. 21202

1970

APPLIED MICROBIOLOGYVOLUME 19 * 1970

EDITORIAL BOARD

J. C. Sylvester, Editor-in-Chief (1972)Abbott Laboratories, North Chicago, Ill.

Albert Balows, Editor (1972)National Comunicable Disease Center,

Atlanta, Ga.

Marvin P. Bryant, Editor (1971)University of Illinois, Urbana

Ralph N. Costilow, Editor (1970)Michigan State University, East Lansing

Oldrich K. Sebek, Editor (1972)The Upjohn Co., Kalamazoo

R. F. Acker (1972)Milton J. Allison (1971)A. W. Anderson (1972)Nester Bohonos (1970)Francis F. Busta (1972)Alex Ciegler (1971)Rita R. Colweil (1971)Marjorie A. Darken (1970)R. H. Deibel (1972)A. L. Demain (1971)R. N. Doetsch (1972)John Ehrlich (1971)James D. Fenters (1970)William W. Ferguson (1971)Maxwell Finland (1972)Anthony F. Gaudy, Jr. (1970)S. E. Gilliland (1971)T. C. Grubb (1970)H. Orin Halvorson (1970)

Clifford W. Hesseltine (1971)William L. Hewitt (1972)John J. Iandolo (1971)Henry D. Isenberg (1971)Marvin J. Johnson (1972)S. S. Kalter (1972)Alfred G. Karlson (1972)Leo Kaufman (1972)Lloyd L. Kempe (1970)George E. Kenny (1970)William M. M. Kirby (1972)W. A. Konetzka (1970)Hubert Lechevalier (1970)Joseph Lein (1972)John Liston (1971)Warren Litsky (1972)Robert Mah (1971)Joseph L. Melnick (1970)Willam I. Metzger (1971)

C. F. Niven, Jr. (1970)David Paretsky (1970)D. Perlman (1971)David Pramer (1971)John B. Routien (1971)Carl P. Schaffner (1972)E. L. Schmidt (1971)Warren S. Silver (1970)Louis DS. Smith (1972)S. S. Socransky (1972)Chrstan Stoll (1970)Welton I. Taylor (1970)M. J. Thirumalachar (1970)Joseph P. Truant (1970)Marvin Turck (1971)Hamao Umezawa (1970)George H. Warren (1972)Eugene D. Weinberg (1972)Max R. Zelle (1971)

Robert A. Day, Managing Editor, 4715 Cordell Ave., Betlhesda, Md. 20014

EX OFFICIO

E. M. Foster, President (1969-1970)Donald E. Shay, Secretary

Applied Microbiology, a publication of the American Societyfor Microbiology, 4715 Cordell Ave., Bethesda, Md. 20014, isdevoted to the advancement and dissemination of applied knowl-edge concerning microorganisms. Applied Microbiology is pub-lished monthly, two volumes per year. The subscription price is$30 (foreign, $31) per year. Single copies are $4.00 (foreign,$4.25). Members of the American Society for Microbiology mayreceive Applied Microbiology as part of their dues. Correspond-ence relating to reprints, defective copies, availability of backissues, and lost or late proofs should be directed to the ASMPublications Office, 4715 Cordell Ave., Bethesda, Md. 20014.Correspondence from ASM members relating to membership

R. E. Hungate, Vice-President (1969-1970)H. B. Woodruff, Treasurer

dues, member subscriptions, changes of address, incorrect add:essincorrect joumals, etc., should be directed to the American Societyfor Microbiology, 1913 I St., N.W., Washington, D.C. 20006.Nonmembers of the Society should write to The Williams &Wilkins Co., 428 E. Preston St., Baltimore, Md. 21202, regardinginstitution ofnonmember subscriptions and nonreceipt ofjournals.Published monthly by the ASM at 428 E. Preston St., Baltimore,Md. 21202.

Second-class postage paid at Baltimore, Md. 21202, and at addi-tional mailing offices. Made in the United States of America.Copyright © 1970, American Society for Microbiology.All Rights Reserved.

CONTENTSNo. 1, JANUARY 1970

Antimicrobial Agents and ChemotherapySemisynthetic Coumermycins: Structure-Activity Relationships. K. E. PRICE, D. R.

CHISHOLM, J. C. GODFREY, M. MISIEK, AND A. GOUREVITCH................ 14Susceptibility of Genital Mycoplasmas to Antimicrobial Agents. PETER BRAUN,

JEROME 0. KLEIN, AND EDWARD H. KASS................................ 62Interaction of Staphylococcal Alpha Toxin and the Estrogenic Hormone Diethyl-

stilbestrol. WILLIAM YOTIS AND ZLATKO SAVOV ............................ 113Resistance to Bacterial Infection Induced by Statolon and Pyran. FRANK F. PINDAK.. 188

Clinical Microbiology, Virology, and ImmunologyViral Susceptibility Range of the Fathead Minnow (Pimephales promelas) Poikilo-

thermic Cell Line. JUAN SOLIS AND EMILIO C. MORA........................ 1Acid-Precipitated M Protein Compared with a Column-Eluted M Protein Preparation

from Type 12, Group A Streptococci. ROBERT C. NOBLE AND MAX D. MOODY.... 5Cross-Agglutination Reaction in Candida albicans and Enteric Bacteria. SEYEDEH

GETTNER AND HAMID R. REZAI............................................ 44Method for Isolation and Identification of Corynebacterium vaginale (Haemophilus

vaginalis). WILBUR E. DUNKELBERG, JR., ROMULUS SKAGGS, AND DOUGLAS S.KELLOGG, JR ........................ 47

Potentially Infectious Agents Associated with Shearling Bedpads. I. Effect of Launder-ing with Detergent-Disinfectant Combinations on Polio and Vaccinia Viruses.ROBERT W. SIDWELL, LOUISE WESTBROOK, GLEN J. DIXON, AND WILLIAM F.HAPPICH ....................................53

Comparison of Antistreptolysin 0 Latex Screening Test with the Antistreptolysin 0Hemolytic Test. GEORGE C. KLEIN, CAROLYN N. BAKER, AND MAX D. MOODY.. 60

Detection of Agglutinins in Chickens Infected with JM Leukosis Virus. THEODOREP. ZACHARIA AND MARTIN SEVOIAN ...................................... 71

Polymyxin-Coagulase-Mannitol-Agar. I. Selective Isolation Medium for Coagulase-Positive Staphylococci. D. S. ORTH AND A. W. ANDERSON.................... 73

Miniature Cell Culture Systems for Use in Field Isolation of Viruses: Cell Stability andSensitivity to Selected Laboratory-Propagated Viruses During Storage in Minia-turized Environments. E. FRANK DEIG, Ivo P. RIE, AND HARRY M. S. WATKINS.. 87

Test Method for Determining the Viricidal Activity of Disinfectants Against VesicularStomatitis Virus. HERBERT S. WRIGHT.................................... 92

Inactivation of Vesicular Stomatitis Virus by Disinfectants. HERBERT S. WRIGHT.... 96Isolation of Ether-Resistant Enteroviruses from Sewage: Methodology. MICHAEL F.

DUFF......................................... 120Flow Chart for Safety Testing Monkey Kidney Cell Suspensions Used in Vaccine

Production. V. R. HARRISON........................................... 170Laboratory Identification of Torulopsis glabrata: Typical Appearance on Routine

Bacteriological Media. MELVIN I. MARKS AND ELIZABETH O'TOOLE.......... 184Lid Method for Evaluating Zone of Inhibition of Treated Fabrics. I. S. PRADIP,

L. SHU, AND P. R. CURTIS ................................................ 193Growth of Venezuelan Equine Encephalomyelitis Virus in Human Diploid Cell Strain

WI-38. MORTON REITMAN AND LEONARD GREEN............................ 196

Food and DeteriorationAntimicrobial Action of Some Citrus Fruit Oils on Selected Food-Borne Bacteria.

ROGER DABBAH, V. M. EDWARDS, AND W. A. MOATS.......................... 27Pathogenicity of Salmonella gallinarum After Metabolic Injury by Freezing. K. M.

SORRELLS, M. L. SPECK, AND J. A. WARREN.................................. 39Aflatoxigenic Isolates of Aspergillusflavus from Pecans. H. S. LILLARD, R. T. HANLIN,

AND D. A. LILLARD .. ....... .... ......... ........................... 128

xxi

Effect of Added Moisture on the Heat Resistance of Salmonella anatum in Milk Choco-late. JUDITH C. BARRILE AND J. FRANK CONE................................ 177

Behavior of Salmonella in Sliced Luncheon Meats. J. M. GOEPFERT AND K. C.CHUNG ................................................................. 190

Ecology and TaxonomyUtilization of Methylthio-s-Triazine for Growth of Soil Fungi. DON S. MURRAY,

WALTER L. RIECK, AND J. Q. LYND .......................................... 11Measurement of 14CO2 Assimilation in Soils: an Experiment for the Biological Ex-

ploration of Mars. JERRY S. HUBBARD, GEORGE L. HOBBY, NORMAN H. HOROWITZ,PAUL J. GEIGER, AND FRANK A. MORELLI.................................. 32

Growth Rate of Sphaerotilus in a Thermally Polluted Environment. THOMAS L.BOrr AND THOMAS D. BROCK............................................ 100

Correlation of Toxicity of Fusarium Isolates to Known Toxins: Survey of Tall FescuePasture. S. G. YATES, H. L. TOOKEY, AND J. J. ELLIS........................ 103

Survival of Gram-Negative Bacteria on Plastic Compounded with Hexachlorophene.GERALD F. TAYLOR...................................................... 131

Differentiation of Serratia from Enterobacter on the Basis of Nucleoside Phospho-transferase Production. ANDREA M. DURAND AND DONNA J. BLAZEVIC ........ 134

Differentiation of Clostridium botulinum Types A, B, and E by Pyrolysis-Gas-LiquidChromatography. R. D. CONE AND R. V. LECHOWICH...................... 138

Microbiological Aspects of Ethylene Oxide Sterilization. I. Experimental Apparatusand Methods. K. KERELUK, R. A. GAMMON, AND R. S. LLOYD................ 146

Microbiological Aspects of Ethylene Oxide Sterilization. II. Microbial Resistance toEthylene Oxide. K. KERELUK, R. A. GAMMON, AND R. S. LLOYD.............. 152

Microbiological Aspects of Ethylene Oxide Sterilization. III. Effects of Humidity andWater Activity on the Sporicidal Activity of Ethylene Oxide. K. KERELUK, R. A.GAMMON, AND R. S. LLOYD .............................................. 157

Microbiological Aspects of Ethylene Oxide Sterilization. IV. Influence of Thickness ofPolyethylene Film on the Sporicidal Activity of Ethylene Oxide. K. KERELUK,R. A. GAMMON, AND R. S. LLOYD.......................................... 163

Nutrition Requirements of Pleurotus flabellatus. H. C. SRIVASTAVA AND ZAKIABANO ................................................................... 166

Undescribed Serotype of Salmonella: Salmonella enteritidis ser. Lovelace. SYLVIA F.BARTES AND WILLIAM J. MARTIN ........................................ 172

Device for Ensuring Continuity of Air Supplied to Carbon Dioxide Incubators forTissue Culture. JOHN W. KREIDER........................................ 174

Potentially Pathogenic, Nonfermentative, H2S-Producing Gram-Negative Rod (I b).ALEXANDER VON GRAEVENITZ AND GERALD SIMON ........................... 176

Occurrence of the Ciliate Protozoa BUtschlia parva Schuberg in the Rumen of theOvine. B. A. DEHORITY................................................ 179

Improved Methodology for Isolating Soil Microorganisms. H. D. TRESNER AND JEANA. HAYES ............................................................... 186

Identification of Mycobacterium kansasii by Susceptibility to Hydroxylamine. HOWARDGRUFF AND MARY OSTERHOUT ............................................ 199

Inhibition of Autoagglutination of Moraxella bovis by 10 % Magnesium Chloride.GEORGE W. PUGH, JR., AND DAVID E. HUGHES................................ 201

Portable Sampler for Microorganisms in Incinerator Stack Emissions. DAVID H.ARMSTRONG............................................................. 204

Metabolism and ProductsEffects of Light Intensity and Thickness of Culture Solution on Oxygen Production by

Algae. R. L. SHULER AND W. A. AFFENS.................................. 76Detection of Griseofulvin and Dechlorogriseofulvin by Thin-Layer Chromatography

and Gas-Liquid Chromatography. R. J. COLE, J. W. KIRKSEY, AND C. E. HOLA-DAY................................................................... 106

Production ofthe Monamycins, Novel Depsipeptide Antibiotics. M. J. HALL AND C. H.HASSALL................................... 109

Effects of Hypobaric and Hyperbaric Helium Atmospheres on the Growth of Chlorellasorokiniana. D. M. ORCUTT, B. RICHARDSON, AND R. D. HOLDEN ..... ....... 182

xxii

No. 2, FEBRUARY 1970

Antimicrobial Agents and Chemotherapy

Determination of Untreated Whole-Milk Effects on In Vitro Antibacterial Activity.J. P. vAN NATTA, P. W. Lo, AND T. S. CHANG.............................. 220

In Vitro Antibacterial Activity of Minocycline and Effect of Agar Medium Utilized inIts Susceptibility Testing. J. A. WASHINGTON II, PAULINE K. W. Yu, AND WILLIAMJOSEPH MARTIN ........................................................ 259

Spectra of Susceptibility of Neisseria meningitidis to Antimicrobial Agents In Vitro.LEONARD F. DEVINE AND CLINE R. HAGERMAN.............................. 329

Serratia marcescens: Biochemical, Serological, and Epidemiological Characteristics andAntibiotic Susceptibility of Strains Isolated at Boston City Hospital. JAMES N.WILFERT, FRED F. BARRETT, W. H. EWING, MAXWELL FINLAND, AND EDWARDH. KASS........................................................ 345

Serum Antibacterial Activity After Oral Suspensions and Capsules of Triacetyloleando-mycin and Erythromycin Estolate. SAMUEL SASLAW AND HAROLD N. CARLISLE.. 370

Antifungal Activity of Alkylxylylene-Bis-Isothiuronium Chlorides. GAIL SHELLEY,D. HUISINGH, AND P. B. HAMILTON......................................... 392

Clinical Microbiology, Virology, and Immunology

Recovery of Anaerobic Microorganisms from Clinical Specimens in Prereduced MediaVersus Recovery by Routine Clinical Laboratory Methods. M. TALMAGEMCMINN AND JAMES J. CRAWFORD......................................... 207

Assay of Chikungunya Virus in Cell Monolayers by Immunofluorescence. NICHOLASHAHON AND WILLIAM A. HANKINS.......................................... 224

Agglutinin Response of Rabbits to Combined Pasteurella tularensis-Brucella abortusVaccination. JOHN E. NUTTER............................................ 250

Procedures for the Assay of Carbenicillin in Body Fluids. J. BURNETT AND R. SUTHER-LAND......................................................... 264

Mycoplasma Recovery from the Male Genitourinary Tract: Voided Urine Versus theUrethral Swab. J. E. GREGORY AND K. R. CUNDY......................... 268

Selective Medium for Moraxella nonliquefaciens. 0. P. VAN BIJSTERVELD AND K. C.WINKLER 278

Comparison of a Blood Culture System Containing Liquoid and Sucrose with SystemsContaining Either Reagent Alone. RICHARD ROSNER...................... 281

Separation of Treponema pallidum from Tissue Debris Through Continuous-ParticleElectrophoresis. JOHN D. SCHMALE, DOUGLAS S. KELLOGG, JR., CURTIS E.MILLER, PATRICIA SCHAMMEL, AND JAMEs D. THAYER........................ 287

Effect of Formalin, j3-Propiolactone, Merthiolate, and Ultraviolet Light Upon InfluenzaVirus Infectivity, Chicken Cell Agglutination, Hemagglutination, and Antige-nicity. MARK A. GOLDSTEIN AND NICOLA M. TAURASO..... ................. 290

Pathogenicity of Clostridium perfringens for Germ-Free Guinea Pigs After Oral In-gestion. RICHARD E. HORTON, DAVID L. MADDEN, AND NORMAN B.MCCULLOUGH........................................... 314

Comparative Poliovirus Permeability of Silver, Polycarbonate, and Cellulose Mem-brane Filters. RICARDO G. HAHN, JACK B. HATLEN, AND GEORGE E. KENNY.... 317

Chikungunya Virus Vaccine Prepared by Tween-Ether Extraction. KENNETH H.ECKELS, VENTON R. HARRISON, AND FRANK M. HETRICK ..................... 321

Passage of Treponema pallidum Through Membrane Filters of Various Pore Sizes.FRANCIS W. CHANDLER, JR., AND JOHN W. CLARK, JR.326

Preliminary Report on the Development of a Diffusion-in-Gel Method for the Diag-nosis of Ram Epididymitis. DONALD M. MYERS AND ALICIA A. SINIUK....... 335

Plaque Formation by Mumps Virus and Inhibition by Antiserum. THOMAS D. FLANA-GAN AND ALMEN L. BARRON............................................... 360

Type 2 Bovine Adenovirus as an Adventitious Contaminant in Primary Bovine Em-bryonic Kidney Cell Cultures. SASHI B. MOHANTY AND MARY G. LILLE...... 381

Germicidal Effect of Ultraviolet Irradiation on Paper Contaminated with Mycobac-teria. T. W. HUBER, R. ANNE REDDICK, AND G. P. KuBICA.................. 383

xxiii

Combined Medium to Determine Deoxyribonuclease Activity and Phenylalanine De-amination by Enterobacteriaceae. THOMAS R. OBERHOFER AND LOUISE MADDOX.. 385

Short-Term Storage at 4 C of Trypsinized Tissues. PETER G. PROBST AND HENRYC. ORR................................................................. 387

Chikungunya Virus Infection of Cell Monolayers by Cell-to-Cell and ExtracellularTransmission. NICHOLAS HAHON AND W. DOUGLAS ZIMMERMAN............ 389

Instability Effects in Diffusion Analyses of Antimicrobial Activity. J. P. VAN NATTA,B. J. Wu, AND T. S. CHANG............................................... 394

Modification of the Kaolin Serum Treatment Used in the Hemagglutination-InhibitionTest for California Arbovirus Encephalitis. P. R. ROBINSON, M. R. ALLEN, AND M.MARCOVICI.............................................................. 398

Food and Deterioration

Spoilage Bacteria in Canned Foods. II. Sulfide Spoilage Bacteria in Canned Mushroomsand a Versatile Medium for the Enumeration of Clostridium nigrificans. CHAU-CHING LIN AND KUAN-CHUNG LIN........ 283

Radiation Treatment of Foods. II. Public Health Significance of Irradiation-RecycledSalmonella. N. A. EPPS AND EDMUND S. IDZIAK.......................... 338

Growth from Spores of Clostridium perfringens in the Presence of Sodium Nitrite.RONALD G. LABBE AND CHARLES L. DUNCAN................................ 353

Ecology and Taxonomy

Response of Airborne Mycoplasma pneumoniae to Abrupt Changes in Relative Hu-midity. M. T. HATCH, D. N. WRIGHT, AND G. D. BAILEY.................... 232

Accumulation of Escherichia coli by the Northern Quahaug. V. J. CABELLI ANDW. P. HEFFERNAN........................................................ 239

Relationship Between Atmospheric Temperature and Survival of Airborne Bacteria.RICHARD EHRLICH, SOL MILLER, AND ROBERT L. WALKER.................... 245

Comparative Enumeration of Lipophilic and Nonlipophilic Cutaneous Diphtheroidsand Cocci. RODNEY F. SMITH........................... ..................254

Bacterial, Fungal, and Actinomycete Populations in Soils Receiving Repeated Appli-cations of 2,4-Dichlorophenoxyacetic Acid and Trifluralin. F. W. BREAZEALEAND N. D. CAMPER....................................................... 379

Metabolism and Products

Effects of Pesticides on Nitrite Oxidation by Nitrobacter agilis. C. L. WINELY ANDC. L. SAN CLEMENTE................................... ................. 214

Effects of N2-02 and C02-02 Tensions on Growth of Fungi Isolated from DamagedFlue-Cured Tobacco. H. YANG AND G. B. LUCAS.......................... 271

Hydrolysis of Conjugated Bile Acids by Cell-Free Extracts from Aerobic Bacteria.DAVID W. YESAIR AND PHILIP HIMMELFARB................................ 295

Herbicide Transformation. I. Studies with Whole Cells of Fusarium solani. R. P.LANZILOTTA AND DAVID PRAMER.......................................... 301

Herbicide Transformation. II. Studies with an Acylamidase from Fusarium solani.R. P. LANZILOTTA AND DAVID PRAMER..................................... 307

Colorimetric Assay for Lysine Decarboxylase in Escherichia coli. WANDA W.-W. LuAND M. F. MALLETTE ..................................................... 367

Production of Hydrocinnamic Acid by Clostridia. C. WAYNE MOSS, M. A. LAMBERT,AND D. J. GOLDSMITH..................................................... 375

No. 3, MARCH 1970

Antimicrobial Agents and ChemotherapyNew Antibacterial Agent Isolated from the Avocado Pear. I. NEEMAN, A. LIFSHITZ,

AND Y. KASHMAN....................................................... 470Action of Diethylstilbestrol on Staphylococci: Further Observations on the Effect of

Resting Cells. WILLIAM W. YOTIS AND SUDHAKER I. BAMAN................. 474Microbiological Assay and Tissue Distribution of 3-Thioguanine Deoxyriboside in

Mice. ROBERT F. PITTILLO AND CAROLYN WOOLLEY......................... 538

xxiv

Clinical Microbiology, Virology, and Immunology

Simple Disposable Method for Quantitative Cultures of Urine. E. JACK BENNER. . 409Positive Fluorescent Treponemal Antibody Reactions in Diabetes. M. KATHRYN

HUGHES, MATTHEW H. FUSILLO, AND BOB S. ROBERSON...................... 425Comparison of Media for Direct Isolation and Transport of Shigellae from Fecal

Specimens. GEORGE K. MORRIS, JUDITH A. KoEHLER, EUGENE J. GANGAROSA,AND ROBERT G. SHARRAR................................................ 434

Ten-Minute Test for Differentiating Between Klebsiella and Enterobacter Isolates.JOHN M. MATSEN....................................................... 438

Thermal Inactivation of Newcastle Disease Virus. G. A. DIGIOIA, J. J. LICCIARDELLO,J. T. R. NICKERSON, AND S. A. GOLDBLITH................................. 451

Effect of Temperature on Radiosensitivity of Newcastle Disease Virus. G. A. DIGIOiA,J. J. LICCIARDELLO, J. T. R. NICKERSON, AND S. A. GOLDBLITH................ 455

Unusual Sphaerophorus Species from the Large Intestine of Man. TED A. PEARSONAND EDWARD BALISH................................................... 458

In Vitro and In Vivo Studies of Streptomycin-Dependent Cholera Vibrios. OSCARFELSENFELD, ANNA STEGHERR-BARRIos, EVA ALDOVA, JAMES HOLMES, ANDMARSHALL W. PARROTT.................................................. 463

Variables of the Rubella Hemagglutination-Inhibition Test System and Their Effect onAntigen and Antibody Titers. NATHALIE J. SCHMIDT AND EDWIN H. LENNETTE... 491

Description of a Viral Agent Found in Blood Obtained from Patients with InfectiousHepatitis. W. V. HARTWELL, MARE P. EIDENBOCK, B. L. MURPHY, AND A. H.AUERNHEIMER........................................................... 521

Adaptation of a Roller-Tube Apparatus to Accommodate Cultivation of Cells inRoller-Bottles. JOSEPH L. ROGERS AND HENRY C. ORR ........................ 541

Use of Vero Cells for Titration of Rinderpest Virus and Its Neutralizing Antibody.H. MIRCHAMSY, A. SHAFYI, AND S. BAHRAMI................................ 545

Evaluation of a New Incubation-Type Indole Strip Test. DONNA J. BLAZEVIC, GRACEMARY EDERER, AND JOHN M. MATSEN..................................... 547

Cell-Associated Nature of Cottontail Rabbit Herpesvirus In Vitro. KENNETH D.LEY AND DIETER BURGER................................................. 549

Food and DeteriorationSalmonellae in Fish Meal Plants: Relative Amounts of Contamination at Various

Stages of Processing and a Method of Control. GEORGE K. MORRIS, WILLIAMT. MARTIN, WILLIAM H. SHELTON, JOY G. WELLS, AND PHILIP S. BRACHMAN ..... . 401

Relation of the Heat Resistance of Salmonellae to the Water Activity of the Environ-ment. J. M. GOEPFERT, I. K. ISKANDER, AND C. H. AMUNDSON............... 429

Ecology and TaxonomyLethal Effects of Electric Current on Escherichia coli. A. PAREILLEUX AND

N. SICARD................................................ 421Formic Hydrogenlyase Induction as a Basis for the Eijkman Fecal Coliform Concept.

CHARLES W. HENDRICKS............................. ......................441Effect of Four Organophosphorus Insecticides on Microbial Activities in Soil.

C. M. TU............................................................. 479Relationship Between Rumen Ammonia Levels and the Microbial Population and

Volatile Fatty Acid Proportions in Faunated and Defaunated Sheep. J. R.MALES AND D. B. PURSER................................................ 485

Motile Nocardoid Actinomycetales. R. S. SUKAPURE, MARY P. LECHEVALIER, H.REBER, M. L. HIGGINS, H. A. LECHEVALIER, AND H. PRAUSER................. 527

Description of Oerskovia gen. n. to Harbor Orskov's Motile Nocardia. H. PRAUSER,MARY P. LECHEVALIER, AND H. A. LECHEVALIER ............................ 534

Agar Dish Isopiestic Equilibration Method for Controlling the Water Potential ofSolid Substrates. R. F. HARRIS, W. R. GARDNER, A. A. ADEBAYO, AND L. E.SOMMERS ................................................................536

Metabolism and ProductsGrowth of Histoplasma capsulatum. VI. Maintenance of the Mycelial Phase. LEO

PINE ................................................................ 413xxv

Fluorimetric Technique for Monitoring Changes in the Level of Reduced Nicotina-mide Nucleotides in Continuous Cultures of Microorganisms. D. E. F. HAR-RISON AND BRITTON CHANCE.............................................. 446

Dehydrogenase Patterns in the Study of Bacteroidaceae. KENNETH C. KEUDELL ANDHERBERT S. GOLDBERG................................................. 505

Use of Glutamic Acid to Supplement Fluid Medium for Cultivation of Bordetellapertussis. A. G. LANE .................................................. 512

Influence ofpH and Aeration on Bacterial Formation of Amylase. Luis A. MAZZAAND RODOLFO J. ERTOLA................................................. 535

Conditions for Production of Ochratoxin A by Aspergillus Species in a SyntheticMedium. MINGTAN LAI, G. SEMENIUK, AND C. W. HESSELTINE............... 542

Continuous Monitoring of Oxygen Concentrations in Air-Purged Shake-Flask Fer-mentations. PHILLIP H. HODSON......................................... 551

No. 4, APRIL 1970Antimicrobial Agents and Chemotherapy

Microbioassay of Antimicrobial Agents. HAROLD J. SIMON AND E. JONG YIN...... 573Pulmonary Absorption and Retention of Kanamycin After Repeated Inhalation Ad-

ministration of Kanamycin Aerosol. RICHARD H. TESKE AND STEPHEN B. MILLER. 583Regression Curve Analysis of Cephalosporin Activity. JOHN A. WASHINGTON II

AND PAULINE K. W. Yu ................................................. 589Effect of pH of Medium and Size of Inoculum on Activity of Antibiotics Against Group

D Streptococcus (Enterococcus). PUBLIO TOALA, CLARE WILCOX, AND MAXWELLFINLAND................................................................ 629

Clinical Microbiology, Virology, and ImmunologyArbovirus Identification by an Agar-Gel Diffusion Technique. C. H. CALISHER AND

K. S. C. MANESS ....................................................... 557Evaluation of Three Cell Culture Systems as Substrates for Influenza Virus Assay.

MARK A. GOLDSTEIN, NICOLA M. TAURASO, AND HENRY C. ORR.............. 580Laboratory Identification of Aeromonads from Man and Other Animals. GENEVIEVE

S. NYGAARD, MARJORIE L. BISSETT, AND RONALD M. WOOD .................. 618Automated Microtitration Test for Antistreptolysin 0. J. M. S. DIXON AND J. J.

GROCHOLSKI ............................................................. 621Airborne Stability of Tailless Bacterial Viruses S-13 and MS-2. EDWARD J. DUBOVI

AND THOMAS G. AKERS................................................... 624Large-Quantity Production of Chicken Embryo Tracheal Organ Cultures and Use in

Virus and Mycoplasma Studies. J. D. CHERRY AND D. TAYLOR-ROBINSON.... 658Virus Particle Content of Smallpox Vaccines. R. C. DUNLAP................ 689Long-Term Preservation of Anaplasma marginale in a Liquid Nitrogen Respository.

JOHN N. LOVE.......................................................... 700Evaluation of Reagent-Impregnated Coagulase-Mannitol Test Strip for Speciation of

Staphylococci. JOHN A. WASHINGTON II AND PAULINE K. W. Yu............ 702Errors Related to Different Techniques of Intraperitoneal Injection in Mice. V. ARIOLI

AND E. RoSsI ........................................................... 704

Food and DeteriorationChemical States of Bacterial Spores: Heat Resistance and Its Kinetics at Inter-

mediate Water Activity. GORDON ALDERTON AND NEVA SNELL.............. 565Mycotoxicity of Aspergillus ochraceus to Chicks. BEN DOUPNIK, JR., AND JOHN C.

PECKHAM................................................................ 594Production of Rubratoxin B by Penicillium purpurogenum Stoll. SHINSAKU NATORI,

SETSUKO SAKAKI, HIROSHI KURATA, SHUN-ICHI UDAGAWA, MASAKATSU ICHINOE,MAMORU SAITO, MAKOTA UMEDA, AND KOHaCHIRO OHTSUBO................. 613

Effect of Spermine on Host-Cell Lysis and Reproduction by a Lactic Streptococcal Bac-teriophage. J. M. ERSKINE............................................... 638

Enzymatic Removal of Diacetyl from Beer. II. Further Studies on the Use of DiacetylReductase. T. N. TOLLS, J. SHOVERS, W. E. SANDINE, AND P. R. ELLIKER...... 649

Apparatus for the Determination of Heat Resistance of Spores. OLIVIER CERF,GERARD GROSCLAUDE, AND DANIEL VERMEIRE.............................. 696

xxvi

Production of High Titers of Enterotoxins for the Routine Testing of Staphylococci.AUDREY W. JARVIS AND R. C. LAWRENCE.................................... 698

Ecology and TaxonomyAerosol Survival of Escherichia coli B Disseminated from the Dry State. C. S. Cox.. 604Nitrogen Requirements and Uricolytic Activity of Cutaneous Bacteria. RODNEY F.

SMITH.................................................................. 643Aerobic Heterotrophic Bacterial Populations of Sewage and Activated Sludge. IV.

Adaptation of Activated Sludge to Utilization of Aromatic Compounds. T. B. S.PRAKASAM AND N. C. DONDERO.......................................... 663

Aerobic Heterotrophic Bacterial Populations of Sewage and Activated Sludge. V. Analy-sis of Population Structure and Activity. T. B. S. PRAKASAM AND N. C. DONDERO.. 671

Characteristics of a New Strain of Bacillus popilliae Sporogenic In Vitro. E. S.SHARPE, GRANT ST. JULIAN, AND CLARENCE CROWELL ........................ 681

Salmonella enteritidis Serotype 501,2, 3:Z4, Z24: -. MARY M. BALL, WILLIAM JEFFREYMARTIN, MIGUEL KOURANY, AND SAM R. TELFORD, JR....................... 694

Detection of Aerobacter aerogenes by Labeling with Radioactive Phosphorus. ROBERTA. MACLEOD, LLOYD A. WHITE, AND J. F. CURRIE ............................ 701

Metabolism and ProductsNaphthylamidase Activity of Leptospira. GLENNA BURTON, D. C. BLENDEN, AND H. S.

GOLDBERG .............................................................. 586Production of Amylase in Liquid Culture by a Strain of Aspergillus oryzae. A. K.

KUNDU AND S. DAS..................................................... 598Formation of Hydrogen Peroxide by Group N Streptococci and Its Effect on Their

Growth and Metabolism. R. F. ANDERS, D. M. HOGG, AND G. R. JAGO........ 608

No. 5, MAY 1970

Antimicrobial Agents and ChemotherapyHaloprogin: a Topical Antifungal Agent. E. F. HARRISON, P. ZWADYK, JR., R. J.

BEQUETTE, E. E. HAMLOW, P. A. TAVORMINA, AND W. A. ZYGMUNT............ 746Chemotherapy of Mice Experimentally Infected with Shigella flexneri. N. K. DAS

AND R. E. CHAMBERLAIN.................................................. 776Effects of w-Amino Acids and Related Compounds on Staphylococcal Infections in

Mice: a Combined Prophylactic-Therapeutic Procedure. YOSHIKI TSUCHIYA,KINJI TANAKA, ELTON S. COOK, AND LEO G. NUTINI......................... 813

Low Antibiotic Potency of Isohumulone. MICHAEL TEUBER ..................... 871

Clinical Microbiology, Virology, and ImmunologyEffect of Chloramphenicol on Host-Bacteriophage Relationships in the Lactic Strep-

tococci. J. M. ERSKINE................................................... 707Changes in the Differential Leukocyte Count of Chicks Inoculated with Salmonella.

E. L. ANDERSON AND J. F. STEPHENS....................................... 726Persistence of an Escherichia coli-Shigella flexneri Hybrid in the Intestinal Tract of

Macaca mulatta. E. STATEN WYNNE, DUANE M. HENRIKSON, AND GEORGE T.DAYE, JR................................................................ 731

Titration of Cholera Antitoxin in Human Sera by Microhemagglutination with For-malinized Erythrocytes. H. DONALD HOCHSTEIN, JOHN C. FEELEY, AND WALLISE. DEWITT.............................................................. 742

Dengue Virus Plaque Development in Simian Cell Systems. I. Factors InfluencingVirus Adsorption and Variables in the Agar Overlay Medium. THOMAS B.STIM.................................................................... 751

Dengue Virus Plaque Development in Simian Cell Systems. II. Agar Variables andEffect of Chemical Additives. THOMAS B. STIM.............................. 757

Gamma-Irradiated Venezuelan Equine Encephalitis Vaccines. MORTON REITMAN,H. R. TRIBBLE, JR., AND LEONARD GREEN................................... 763

xxvii

Histopathology of Listeria monocytogenes After Oral Feeding to Mice. JOHN K.MILLER AND JEAN BURNS................................................. 772

Method for Extracting Viral Hemagglutination-Inhibiting Antibodies from the Non-specific Inhibitors of Serum. WILLIAM A. ALTEMEIER III, FRANCIS K. MUNDON,FRANKLIN H. Top, JR., AND PHILIP K. RUSSELL.............................. 785

Viremia and Virus Measurements of Rabbit Pox in CV- 1 Cells. CHENG T. CHO,THOMAS LOCKE, AND HERBERT A. WENNER................................... 791

Ultraviolet Devitalization of Eight Selected Enteric Viruses in Estuarine Water.WILLIAM F. HILL, JR., FREDERICK E. HAMBLET, WILLIAM H. BENTON, AND ELMERW. AKIN.......................................... ..................... 805

Somatic Antigens of Streptococcus Group E. I. Comparison of Extraction Techniques.JERRY B. PAYNE AND CHARLES H. ARMSTRONG............................... 818

Somatic Antigens of Streptococcus Group E. II. Separation and a Partial Physico-chemical Characterization. JERRY B. PAYNE AND CHARLES H. ARMSTRONG...... 823

Application of Agglutinins for the Rapid and Accurate Identification of MedicallyImportant Candida Species. CHARLES E. SWEET AND LEO KAUFMAN............ 830

Fowl Cholera Immunization in Turkeys. I. Efficacy of Various Cell Fractions ofPasteurella multocida as Vaccines. JOHN BROWN, DONALD L. DAWE, RICHARDB. DAVIS, JOHN W. FOSTER, AND K. K. SRIVASTAVA.......................... 837

Fowl Cholera Immunization in Turkeys. II. Use of Experimental Epornitic Method toStudy Vaccine Efficacy in Flocks of Turkeys. DONALD L. DAWE, RICHARD B.DAVIS, JOHN BROWN, JOHN W. FOSTER, AND K. K. SRIVASTAVA................ 842

Fowl Cholera Immunization in Turkeys. III. Significance of Market Quality in theEvaluation of Fowl Cholera Vaccines. RICHARD B. DAVIS, JOHN BROWN, DONALDL. DAWE, JOHN W. FOSTER, AND K. K. SRIVASTAVA.......................... 844

Thermoelectrically Cooled Temperature-Gradient Apparatus for Comparative Celland Virus Temperature Studies. H. F. CLARK, F. KAMINSKI, AND D. T. KARZON. 848

Accuracy of Smallpox Diagnosis by Immunofluorescence with a Purified Conjugate.JOHN NOBLE, JR., AND MARY S. LOGGINS.................................... 855

Enhancement by Poly-D-Lysine of Poly I: C-Induced Interferon Production in Mice.J. M. RICE, W. TURNER, M. A. CHIRIGOS, AND N. R. RICE.................... 867

Prevention of Oxidation of Transport Media. JOHN P. SMITH..................... 870

Food and Deterioration

Bacteriological and Shelf-Life Characteristics of Canned, Pasteurized Crab Cake Mix.PAISAN LOAHARANU AND ANTHONY LOPEZ................................... 734

Bacteriological Examination of Commercial Precooked Eastern-Type Turkey Rolls.A. J. MERCURI, G. J. BANWART, J. A. KINNER, AND A. R. SESSOMS.............. 768

Effects of Moisture Content and Temperature on Aflatoxin Production in Corn.HUGH L. TRENK AND PAUL A. HARTMAN.................................. 781

Salmonella Contamination in a Poultry-Processing Plant. GEORGE K. MORRIS ANDJOY G. WELLS........................................................... 795

Fungal Disfigurement of Paper, and Soft Rot of Cedar Shingles. D. E. EVELEIGH ... 872Simple Method for Collection of Samples from a Frozen Food. D. M. ADAMS AND

F. F. BUSTA............................................................. 878

Ecology and Taxonomy

Black Beauty Out of Mycobacterium fortuitum Cruz. RUTH E. GORDON AND C.HOR-NAY PANG.................................. .........................862

Recovery of Airborne Streptococcal L-Forms at Various Relative Humidities.R. H. STEWART AND D. N. WRIGHT......................................... 865

Metabolism and Products

Degradation of Urea Herbicides by Cell-Free Extracts of Bacillus sphaericus. P. R.WALLNOFER AND J. BADER................................................. 714

Production of Chlorflavonin, an Antifungal Metabolite of Aspergillus candidus.J. E. MUNDEN, D. BUTTERWORTH, G. HANSCOMB, AND M. S. VERRALL.......... 718

xxviii

Metabolism of Tryptophan by Pseudomonas aureofaciens. III. Production of SubstitutedPyrrolnitrins from Tryptophan Analogues. R. L. HAMILL, R. P. ELANDER, J. A.MABE, AND M. GORMAN.................................................. 721

Influence of Dissolved Oxygen Levels on Production of L-Asparaginase and Pro-digiosin by Serratia marcescens. BERNARD HEINEMANN, ALMA J. HOWARD,AND HENRY J. PALOCZ.................................................... 800

Construction of a Laboratory Press for Use with the French Pressure Cell. G. J.VANDERHEIDEN, A. C. FAIRCHILD, AND G. R. JAGO.......................... 875

Degradation of Endrin, Aldrin, and DDT by Soil Microorganisms. K. C. PATIL,F. MATSUMURA, AND G. M. BOUSH......................................... 879

No. 6, JUNE 1970

Antimicrobial Agents and Chemotherapy

Comparison of Cephalexin, Penicillin V, and Ampicillin in Streptococcal Infections inMonkeys. SAMUEL SASLAW AND HAROLD N. CARLISLE..................... 943

Bactericidal Activity of a Broad-Spectrum Illumination Source. PHILIP HIMMELFARB,ARTHUR SCOTT, AND PHILIP S. THAYER................................... 1013

Device for Turbidity Standardizing of Cultures for Antibiotic Sensitivity Testing.JOANNE E. STEMPER AND JOHN M. MATSEN............................... 1015

Antibacterial Effect of Some Azaphenanthrene Compounds. K. G. GUPTA, S. V.KESSAR, AND BALDEV SINGH................................. 1017

Method for Quantitating Formaldehyde Gas in Air. D. T. BRAYMEN AND J. R.SONGER................................. 1021

Clinical Microbiology, Virology, and Immunology

Preparation of Agglutinating Antisera and Fluorescent-Antibody Conjugates AgainstPasteurella tularensis in Equines. JAMES H. GREEN, RICHARD C. BOLIN, RUSSELLK. CARVER, HERMAN GROSS, NAN PIGOTT, AND WILLIAM K. HARRELL....... 894

Automated Fluorescent Treponemal Antibody Test: Instrument and Evaluation.JOEL S. LEwiS, WILLIAM P. DUNCAN, AND GENEVIEVE W. STOUT............ 898

Partial Substitution of Serum in Hematopoietic Cell Line Media by Synthetic Polymers.A. MIZRAHI AND G. E. MOORE.......................................... 906

Pesticinogeny: a Characteristic Useful for Presumptive Identification and Isolation ofPasteurella pestis. EARL D. BEESLEY AND MICHAEL J. SURGALLA .......... 915

Identification of Staphylococci Isolated from Clinical Material. E. A. RAYMOND ANDW. H . TRAUB.......................................................... 919

Purified Rabies Vaccine (Suckling Rat Brain Origin). J. F. LAVENDER ............ 923Studies of Laryngotracheitis Virus in Avian Tissue Cultures. III. Enhancement of In-

fectivity by Diethylaminoethyl-Dextran. C. R. ROSSI AND A. M. WATRACH.... 932Rapid Fluorescent-Antibody Stain Technique with Group A Streptococci. PAT W.

FREEBURG......................................................... ......940Quantitative Studies on Fabrics as Disseminators of Viruses. IV. Virus Transmission

by Dry Contact of Fabrics. ROBERT W. SIDWELL, GLEN J. DIXON, LOUISEWESTBROOK, AND FLORENCE H. FORZIATI.................................. 950

Amphotericin B Serum Concentrations During Therapy. BRANCH T. FIELDS, JR.,JOSEPH H. BATES, AND ROBERT S. ABERNATHY.............................. 955

Plaque Assay for Q Fever and Scrub Typhus Rickettsiae. JOSEPH E. MCDADE ANDPETER J. GERONE....................................................... 963

Application of Freeze-Etching Method to the Study of Reovirus-Infected LLC-MK2Cells. K. R. ROZEE AND K. B. EASTERBROOK............................. 997

Dubos Oleic Acid Agar Medium in the Differentiation of Meningococci and Gono-cocci. B. B. DIENA, R. WALLACE, C. P. KENNY, AND L. GREENBERG. 1025

Food and Deterioration

Enzymatic Removal of Diacetyl from Beer. III. Enzyme Protection and Regenerationof Cofactor. JANET W. THOMPSON, J. SHOVERS, W. E. SANDINE, AND P. R.ELLIKER....................................................... 883

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Concentrated Cultures of Leuconostoc citrovorum. S. E. GILLILAND, E. D. ANNA,AND M. L. SPECK ................. 890

Use of a Titrimetric Method to Assess the Bacterial Spoilage of Fresh Beef. LEORAA. SHELEF AND JAMES M. JAY ........................................... 902

Relationship Between Staphylococcal Antiserum Titer and Zone Development on Im-mune Serum Plates. K. F. WEISS AND RUTH N. ROBBINS ............. 911

Survival and Activity of Frozen Starter Cultures for Cheese Manufacture. BARBARAP. KEOGH............................................ 928

Hydrogen Sulfide Production by Bacteria and Sulfmyglobin Formation in PrepackedChilled Beef. D. J. NICOL, M. K. SHAW, AND D. A. LEDWARD ........... 937

Influence of Water Activity on the Growth of Clostridium perfringens. DOROTHY H.STRONG, EDITH F. FOSTER, AND CHARLES L. DUNCAN ............... 980

Viability Control for Mildew Testing of Materials and Equipment in TropicalChambers. RICHARD T. DARBY .......................................... 1007

Ecology and Taxonomy

Microbial Biomass on Particulate Organic Matter in Seawater of the Euphotic Zone.HUMITAKE SEKI......................................................... 960

Occurrence of Purple Sulfur Bacteria in a Sewage Treatment Lagoon. H. W. HOLMAND J. W. VENNES........................ 988

Laboratory Measure of an Ecosystem Response to a Sustained Stress. ROBERT A.COLER AND HAIM B. GUNNER.......................................... 1009

Improved Automatic Inoculator for Bacterial Cultures. S. MAIER..... 1023

Metabolism and Products

Bacteriology of Manganese Nodules. IV. Induction of an MnO2-Reductase System in aMarine Bacillus. R. B. TRIMBLE AND H. L. EHRLICH........................ 966

Volutin Granules in Zoogloea ramigera. FRANK A. ROINESTAD AND IRVING YALL.. 973Cytochemical Electron Microscopic Localization of Esterase Activity in Lactobacillus

casei. J. P. BROWN ........................... ........................ 1001Nuclear Distribution in the Mycelium of Claviceps and the Problem of Strain Selection.

D. HAREVEN AND Y. KOLTIN ............................. ....... .. 1005Variation in Composition of Yeast Phosphohexosans. M. E. SLODKI, M. J. SAFRANSKI,

D. E. HENSLEY, AND G. E. BABCOCK..................................... 1019

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