Application of a real-time quantitative LAMP assay for the detection of Phytophthora infestans airborne inoculum

Melissa Si-Ammour

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Università Cattolica del Sacro Cuore - Piacenza, Italy

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Objective: Test the suitability of the real-time LAMP assay for a rapid detection and quantification of airborne inoculum of P. infestans

Monitoring airborne inoculum of P. infestans as a complement to decision support systems (DSS)

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Rotating arm spore sampler (Phytodata Inc.)

• Inoculum quantity and dispersal of P. infestans are considered a key factor in potato late blight epidemic development.

• Laboratory validation based on Real-time PCR quantification by Fall et al. (2015)

• Loop Mediated Isothermal Amplification (LAMP) assay for the detection of P. infestans adapted for on-site use by Si Ammour et al. (2017).

Fall et al. Plant Pathology (2015) 64, 178–190Si Ammour et al. Plant disease (2017), 101(7), 1269-1277

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Laboratory validation of the quantitative real-time LAMP assay

Real-time qPCR machine Smart-DART portable device

R2= 0,96N=23

R2= 0,86N=12

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Impact and Prospectum

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• Proof of concept for the suitability of real-time LAMP for the quantification of P. infestans airborne sporangia

• Field validation is needed, coupled with a spore-sampling network

• Adaptation of an on-site extraction method for use with the Smart-DART device

• Define action threshold based on airborne inoculum concentration

Smart-Dart platform (Diagenetix Inc., Honolulu, HI, USA)

Thank Youvia Emilia Parmense, 84. 29122- Piacenza, Italy

Melissa.siammour@unicatt.it

(+39) 0523 59 92 52

Melissa Si-Ammour

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Application of a real-time quantitative LAMP assay for the detection of Phytophthora infestans airborne inoculum

Melissa Si-Ammour, David Mathieu Tremblay, Guillaume J. Bilodeau, Hervé Van Der Heyden, Thaer Yaseen, Leonardo Varvaro, and Odile Carisse