Embed Size (px)
Citation preview
©Biotage 2019
Page 1
Figure 1.
ISOLUTE 101
simazine, Figure 1
thiobencarb, Figure 1
thiuram, Figure 1
3
46 59 5
6
ISOLUTE 101
Figure 2
ODS
46
59 5 6
ISOLUTE 101 100 mL/min
Application Note AN190520
Simazine, CAS: 122-34-9
Thiobencarb, CAS: 28249-77-6
Thiuram, CAS: 137-26-8
ISOLUTE 101 200 mg/6 mL, part number 101-0020-C
200 mL 10 %EDTA 2Na 4 mL
pH 3.5
5 mL 10 %EDTA 2Na 4 mL 15
mL
100 mL/min
10 mL 100 mL/
min 30
5 mL
5 mL
35
1 mL 5
mL 35 1
mL 10 mg/L -d10
20 µL 5,000 mg/L PEG PEG 200 PEG 300 1:1
20 µL GC/MS
35
1 mL
HPLC/UV
Figure 2. ISOLUTE 101
©Biotage 2019
Page 2
GC/MS
Agilent GC7890A 5975C
HP-5 MS 30 m x 0.25 mm, 0.25 µm, Agilent
1.1 mL/min
Table 1
150 ⁰C
280 ⁰C
230 ⁰C
EI
MS
Table 2
1 µL
HPLC/UV
Agilent 1100
XDB-C18 75 mm x 3.0 mm, 3.5 µm, Agilent
40 ⁰C
A:
B:
HPLC
Table 3
272 nm
10 µL
Time (min) 温度
0→1 50 ⁰C
2→11.5 (20 ⁰C/min)
11.5→12.5 280 ⁰C
Table 1. GC
定量用(m/z) 確認用(m/z)
201 173
100 72
188 160
Time (min) %A %B
0→2 80 20
2→8 80→10 20→90
8→12 10 90
ISOLUTE 101
7 20
2 100 mL/min) 15 13 mL/min)
30 15
30
3 9
42 89
©Biotage 2019
Page 3
Figure 3. 0.2 mg/L SIM)
Figure 4. 0.25 mg/L λ 272 nm
Page 4
© 2019 Biotage Japan. All rights reserved. No material may be reproduced or published without the written permission of Biotage. Information in this document is subject to change without notice and does not represent any commitment from Biotage. E&OE. A list of all trademarks owned by Biotage AB is available at www.biotage.com/legal. Other product and company names mentioned herein may be trademarks or registered trademarks and/or service marks of their respective owners, and are used only for explanation and to the owners’ benefit, without intent to infringe. This appli-cation note is intended for research use only, not for diagnostic use.
Table 5. n=5
Figure 5. 0.05~0.5 mg/L 0.1~1.0 mg/L
»
»
Ordering Information
Part Number Description Quantity
101-0020-C ISOLUTE 101 200 mg/6 mL 30
415000 TurboVap®LV 1
41964 TurboVap®LV Multi Rack (48
Positions, 10-20 mm Tubes) 1
SPE2-2616
Biotage® Horizon SmartPrep
II Extractor Base Module
Configured With 6 mL Plung-
er Assembly
1
Part Number: AN190520
µg/L RSD
0.308 2.18 102.5 1.85 85.9
0.295 2.64 98.5 1.62 92.5
0.532 2.68 106.4 1.54 81.7
Table 6. n=5
µg/L RSD
0.318 5.30 106.0
0.325 4.49 108.3
0.520 6.21 104.0
