7/29/2019 Appendix B - Metabolic Inhibitors Used in Virology
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Appendix B: Metabolicinhibitors used in virologyMost inhibi tors
are highly tox ic , and i t is essent ia l that they are used s t r
ic t ly in accordance wi th al lre levant sa fe ty ins t ruc t ions
and procedures .
TypicalMolecular concent ra t ion for
inhibi tor mass Effect useCycloheximide (3-[2-{3,5-dimethyl-
281.42-oxocyclohexyl}-2-hyd roxyethyi]glutarimidePuromycin
(3'-[a-amino-p-methoxycinnamamido]-3'-dec)xy-N,
N-dimethyladenosineCastan osperm ine (8~13-indolizid in e- 1
x,613,7 x,81[3-tetrol)
544.4
189.2
1-Deoxymannojirimycin
(1,5-dideoxy-1,5-imino-D-mannitol)1-Deoxynojirimycin
(1,5-dideoxy-1,5-imino-D-glucitol)Monensin
199.6
163.2692.9
Swainosine
(8(x,13-indolizidine-lc~,2(z,813-triol)Tunicamycin
Actinomycin D
173.2840approximately;very variable,multiple isomers1255.4
Inhibitor of eukaryotic proteinsynthesis at translational
level;used to induce high levels ofmRNA prior to removalProtein
synthesis inhibitor,Disrupts polyribosomesInhibits processing of
high-mannose oligosccharides tocomplex-typeinhibits processing of
high-mannose oligosaccharides tocomplex-typeInhibits formation of
high-mannose oligosaccharidesMonovalent ionophore: Multipleeffects
on glycosylation due todisruption of passage through
theGolgiInhibits processing of high-mannose
oligosaccharidesPrevents N-linked glycosylation
100--360 ~M
200 ~M
530-1320 ~M
1000-40 00 ~I,M
1000-50 00 ~I,M1.0-1.5 ~M
O. 1- 29 ~M1.0-6 I~M
Irreversible inhibitor of transcription 0.4- 8 IJMfrom
double-standard DNA (RNAsynthesis is inhibited at
lowerconcentrations than is DNAsynthesis)
Virology Methods ManualISBN 0-12-465 330-8 Copyright 9 1996
Academic Press LtdAll rights of reproduction in any form
reserved
7/29/2019 Appendix B - Metabolic Inhibitors Used in Virology
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Virology methods manual
MolecularInhibitor mass
. .. . . | | . . . . . . . . . . .
Typicalconcentration for
Effect useAphidicolin 338.5
Phosphonoacetic acid 140.0
(z-Amanitin 919.0
Reversible inhibitor of eukaryoticDNA polymerases cc and t3
andsome viral DNA polymerases. Maybe used to synchronize cell
growthInhibits some viral DNApolymerases (notably inherpesviruses,
where it is used toassay DNA synthesis-dependentprotein
synthesis)Inhibits RNA polymerases II(mRNA) and III (tRNA)
3-20 ~tM
2140 #M
0.001-0.01 I~M(polymerase II)10-100 #M(polymerase III)
Cord ycepi n (3'-deo xyade nosin e) 251.2 Inhibits RNA synthesis
and mRNA 200 ~tMpolyadenylation
, . .. . , m . . . . |. . |. . 9 9 .
This table is derived from Virology Labfax, D.R. Harper (Ed.),
1993, Bios Scientific Publishers, and is presentedhere with
permission of the publishers.
364 Appe ndix B
