AP Biology Discussion Notes

Friday 3/6/2015

Goals for Today• Be able to describe the difference in the 2

+pGLO plates using our Operon Vocabulary

• Be able to describe what it means and why genes from a jellyfish can function in bacteria.

• Be able to analyze transformation efficiency and describe what that calculation actually means.

Question of the day 3/06• What kind of Operon is normally in the

“on” position, how is this accomplished, and what type of control would this exhibit?

• Give an example of this type of operon.

• Is this the operon in our lab

Desired Lab Results

Lab Analysis

• Be able to explain the predictions/results discussing OPERONS!

What is this thing we inserted?

Arabinose Operon

Arabinose Operon

GFPgene

Arabinose Operon

• OPERON VOCABULARY to use in your lab explanation. Describe what is going on in the 2 +pGLO plates using the terms:– Inducible/repressible– Positive/negative control– Promoter– Operator– Repressor Protein– Transcription

GFPgene

Lab Analysis• Be able to explain the predictions/results

discussing OPERONS!

1. Work through lab analysis & calculations together. (Yellow sheet) and ASK QUESTIONS!

2. Next answer white sheet as a group.

What is transformation efficiency?

• What is the unit?

• Is this number bigger or smaller than your number of colonies?

• Did we add a whole µg?

Questions on the Lab?

LAB QUIZ on TUESDAY