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    ChapterChapter 7

    PhotosynthesisPhotosynthesis

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    Chapter 72

    What Is Photosynthesis?What Is Photosynthesis?

    Early cells evolved the abilityEarly cells evolved the abilityto perform photosynthesisto perform photosynthesisabout 2 billion years agoabout 2 billion years ago

    PhotosynthesisPhotosynthesis is the ability tois the ability tocapture sunlight energy andcapture sunlight energy andconvert it to chemical energyconvert it to chemical energy

    Most forms of life on EarthMost forms of life on Earthdepend on the chemicaldepend on the chemicalenergy produced byenergy produced by

    photosynthetic organismsphotosynthetic organisms

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    Chapter 73

    The PhotosyntheticThe PhotosyntheticEquationEquation

    6CO2

    carbon

    dioxide

    + 6H2O

    water

    + light energysunlight

    C6H12O6glucose

    (sugar)

    + 6O2oxygen

    Photosynthesis occurs in plants,Photosynthesis occurs in plants,algae, andalgae, and some prokaryotessome prokaryotes

    Photosynthetic organisms arePhotosynthetic organisms areautotrophsautotrophs (self-(self- feeders)feeders)Photosynthesis in plants occursPhotosynthesis in plants occurswithinwithin chloroplastschloroplasts

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    Chapter 74

    Gases, Sugar, andGases, Sugar, andWater CycleWater CycleProduction of carbonProduction of carbon

    compounds like glucosecompounds like glucose

    (photosynthesis) is linked(photosynthesis) is linkedwith energy extraction (inwith energy extraction (incellular respiration)cellular respiration)

    Water, COWater, CO22, sugar, and O, sugar, and O22

    cycle between the twocycle between the twoprocessesprocesses

    Photosynthesis and cellularPhotosynthesis and cellular

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    Chapter 75

    Photosynthesis &Photosynthesis &RespirationRespiration

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    Chapter 76

    Adaptations forAdaptations forPhotosynthesisPhotosynthesis

    LeavesLeaves

    ChloroplastsChloroplasts

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    Chapter 77

    Structures ofStructures ofPhotosynthesisPhotosynthesis

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    Chapter 78

    LeavesLeavesFlattened leaf shapeFlattened leaf shape

    exposes large surface areaexposes large surface areato catch sunlightto catch sunlight

    Upper and lower leafUpper and lower leaf

    surfaces of a leaf comprisesurfaces of a leaf comprisethethe epidermisepidermis

    Waxy, waterproofWaxy, waterproofcuticlecuticle onon

    outer surfaces reducesouter surfaces reduceswater evaporationwater evaporation

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    Chapter 79

    Leaf AnatomyLeaf AnatomyAdjustable pores calledAdjustable pores calledstomatastomata allow for entry ofallow for entry ofair with COair with CO22

    InnerInner mesophyllmesophyll cell layerscell layerscontain majority ofcontain majority ofchloroplastschloroplasts

    Vascular bundlesVascular bundles (veins)(veins)supply water and mineralssupply water and mineralsto the leaf while carryingto the leaf while carrying

    sugars away from the leafsugars away from the leaf

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    Chapter 710

    Internal Leaf StructureInternal Leaf Structure

    Internal leaf structure isInternal leaf structure is

    crucial to photosynthesiscrucial to photosynthesissince photosynthesissince photosynthesisoccurs primarily in theoccurs primarily in the

    leaves of land plansleaves of land plans

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    Chapter 711

    Leaf StructureLeaf Structure

    MesophylMesophylCellCell

    MesophylMesophylCellCell

    Upper EpidermisUpper EpidermisUpper EpidermisUpper Epidermis

    LoweLowe

    EpidermiEpidermi

    LoweLowe

    EpidermiEpidermiVeinVeinVeinVein StomaStomaStomaStoma

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    Chapter 712

    Anatomy of a ChloroplasAnatomy of a Chloroplas

    Mesophyll cells have 40-Mesophyll cells have 40-

    200 chloroplasts each200 chloroplasts eachChloroplasts are boundedChloroplasts are boundedby a double membraneby a double membrane

    composed of thecomposed of the innerinner andandouter membranesouter membranes

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    Chapter 713

    Anatomy of a ChloroplastAnatomy of a Chloroplast

    TheThe stromastroma is the semi-fluidis the semi-fluidmedium within the innermedium within the innermembranemembrane

    Disk-shaped sacs calledDisk-shaped sacs calledthylakoidsthylakoids found within thefound within thestroma in stacks calledstroma in stacks calledgranagrana

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    Chapter 714

    Chloroplast StructureChloroplast Structure

    Inner & OuterInner & Outer

    MembranesMembranes

    ThylakoidsThylakoids

    GranumGranum

    StromaStroma(space)(space)

    Th E i Vi iblTh E i Vi ibl

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    Chapter 715

    The Energy in VisibleThe Energy in VisibleLightLightThe sun radiatesThe sun radiates

    electromagnetic energyelectromagnetic energyVisible light is radiationVisible light is radiationfalling between 400-750falling between 400-750

    nanometers of wavelengthnanometers of wavelengthPackets of energy calledPackets of energy calledphotonsphotons with different energywith different energy

    levelslevelsShort-wavelength photonsShort-wavelength photonsare very energeticare very energetic

    Lon er-wavelen th hotonsLon er-wavelen th hotons

    16

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    Chapter 716

    Light, ChloroplastLight, ChloroplastPigments, & PhotosynthesisPigments, & Photosynthesis

    Gamma RaysGamma Rays X-RaysX-RaysUVUV InfraredInfraredMicro-Micro-waveswaves RadioRadioWavesWaves

    Visible LightVisible Light

    400400 450450 500500 550550 600600 650650 700700 7575

    Wavelength (nanometers, nm)Wavelength (nanometers, nm)

    17Li ht C t d bLi ht C t d b

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    Chapter 717

    Light Captured byLight Captured byPigmentsPigments

    Action of light-capturingAction of light-capturingpigmentspigmentsAbsorptionAbsorption of certainof certain

    wavelengths (light iswavelengths (light istrapped)trapped)ReflectionReflection of certainof certain

    wavelengths (lightwavelengths (lightbounces back)bounces back)TransmissionTransmission of certainof certain

    wavelengths (lightwavelengths (light

    18Li ht C t d bLi ht C t d b

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    Chapter 718

    Light Captured byLight Captured byPigmentsPigments

    Absorbed light drivesAbsorbed light drivesbiological processes whenbiological processes whenit is converted to chemicalit is converted to chemicalenergyenergy

    Common pigments found inCommon pigments found inchloroplasts include:chloroplasts include:ChlorophyllChlorophyllaaandandbbAccessory pigments suchAccessory pigments such

    asas carotenoidscarotenoids

    h 19

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    Chapter 719Light Captured byLight Captured by

    PigmentsPigmentsChlorophyll a and bChlorophyll a and b absorbabsorb

    violet, blue, and red lightviolet, blue, and red lightbut reflect green lightbut reflect green light(hence they appear green)(hence they appear green)

    CarotenoidsCarotenoids absorb blue andabsorb blue andgreen light but reflectgreen light but reflectyellow, orange, or redyellow, orange, or red

    (hence they appear yellow-(hence they appear yellow-orange)orange)

    Pigment absorbs visiblePigment absorbs visible

    lightlight

    Ch 720

    Absorption SpectraA sorpt on Spectra

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    Chapter 720

    00

    2020

    4040

    6060

    8080

    100100

    %L

    ight

    Absorption

    %L

    ight

    Absorption

    0000 450450 500500 550550 600600 650650 700700 750750

    Wavelength (nanometers)Wavelength (nanometers)

    0000 450450 500500 550550 600600 650650 700700 750750

    Absorption SpectraA sorpt on Spectra

    Visible LightVisible Light

    ChlorophyllChlorophyll

    CarotenoidsCarotenoids

    PhycocyaPhycocyaninnin

    CollectivCollectivelyely

    Chapter7 7Light, ChloroplastLight, ChloroplastPigments &PhotosynthesisPigments &Photosynthesis

    GammaRaysGammaRays XX--RaysRaysUVUV InfraredInfraredMicroMicro--waveswavesRadioRadioWavesWaves

    VisibleLightVisibleLight

    777777 777777 777777 777777 777777 777777 777777 777777

    Wavelength(nanometers)Wavelength(nanometers)

    Ch t 721O i f

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    Chapter 721

    GlucoseGlucose

    OO22

    Overview ofOverview ofPhotosynthesisPhotosynthesis

    Light-DependentLight-DependentReactionsReactions

    (in thylakoids)(in thylakoids)

    Light-IndependentLight-IndependentReactionsReactions

    (in stroma)(in stroma)

    DepleteDepletedd

    CarriersCarriers(ADP,(ADP,

    NADPNADP++))

    DepleteDepletedd

    CarriersCarriers

    (ADP,(ADP,

    NADPNADP++))

    EnergizEnergizeded

    CarriersCarriers

    (ATP,(ATP,

    NADPH)NADPH)

    HH22OO

    CC

    OO22

    Ch t 722

    The PhotosystemsThe Photosystems

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    Chapter 722

    The PhotosystemsThe Photosystems

    Ch t 723Li ht DependentLight Dependent

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    Chapter 723

    Light-DependentLight-DependentReactionsReactions

    Steps of the light reactions:Steps of the light reactions:

    Accessory pigments inAccessory pigments in

    Photosystems absorbPhotosystems absorblight and pass energy tolight and pass energy toreaction centersreaction centers containingcontaining

    chlorophyllchlorophyllReaction centers receiveReaction centers receive

    energized electronsenergized electrons

    Ch t 724Light DependentLight Dependent

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    Chapter 724

    Light-DependentLight-DependentReactionsReactionsEnergized electrons thenEnergized electrons then

    passed down a series ofpassed down a series ofelectron carrierelectron carriermolecules (molecules (ElectronElectron

    Transport ChainTransport Chain))Energy released fromEnergy released from

    passed electrons used topassed electrons used to

    synthesizesynthesize ATPATP from ADPfrom ADPand phosphateand phosphate

    Energized electrons alsoEnergized electrons also

    used to makeused to make NADPHNADPH fromfrom

    Ch t 725

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    Chapter 725

    Thylakoid Structure &Thylakoid Structure &Light-Dependent ReactionsLight-Dependent Reactions

    Ch t 726

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    Chapter 726

    Oxygen as a By-productOxygen as a By-product

    Chapter 727

    ChemiosmosiChemiosmosi

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    Chapter 727

    ChemiosmosiChemiosmosis 1s 1

    Chapter 728

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    Chapter 728

    ChemiosmosChemiosmosis 2is 2

    Chapter 729LightLight InIndependentdependent

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    Chapter 729

    Light-Light-InIndependentdependentReactionsReactions

    NADPH and ATP from light-NADPH and ATP from light-dependent reactions useddependent reactions usedto power glucose synthesisto power glucose synthesis

    Light notLight not directlydirectlynecessarynecessaryfor light-independentfor light-independentreactions if ATP & NADPHreactions if ATP & NADPH

    availableavailableLight-independent reactionsLight-independent reactionscalled thecalled the Calvin-BensonCalvin-Benson

    CycleCycle oror CC33 CycleCycle

    Chapter 730

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    Chapter 730The CThe C33 CycleCycle

    6 CO6 CO22 used to synthesize 1used to synthesize 1glucose (Cglucose (C66HH1212OO66))

    Carbon dioxide is capturedCarbon dioxide is captured

    and linked to ribuloseand linked to ribulosebisphosphate (RuBP)bisphosphate (RuBP)

    ATP and NADPH from lightATP and NADPH from light

    dependent reactions useddependent reactions usedto power Cto power C33 reactionsreactions

    Chapter 731

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    Chapter 731CC33 Cycle Has Three PartsCycle Has Three Parts

    1. Carbon fixation (carbon1. Carbon fixation (carboncapture)capture)

    6 Ribulose bisphosphate6 Ribulose bisphosphate(RuBP) molecules combine(RuBP) molecules combinewith 6COwith 6CO22

    Fixation step andFixation step andsubsequent reactions yieldsubsequent reactions yield

    twelve 3-carbontwelve 3-carbonphosphoglyceric acid (PGA)phosphoglyceric acid (PGA)moleculesmolecules

    Chapter 732

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    Chapter 732CC33 Cycle Has Three PartsCycle Has Three Parts

    2. Synthesis of2. Synthesis ofGlyceraldehyde 3-Glyceraldehyde 3-Phosphate (G3P)Phosphate (G3P)

    Energy is donated by ATPEnergy is donated by ATPand NADPHand NADPH

    Phosphoglyceric acid (PGA)Phosphoglyceric acid (PGA)molecules are convertedmolecules are converted

    into glyceraldehyde 3-into glyceraldehyde 3-Phophate (G3P) moleculesPhophate (G3P) molecules

    Chapter 733

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    Chapter 733CC33 Cycle Has Three PartsCycle Has Three Parts

    3. Regeneration of3. Regeneration ofRibulose bis-phosphateRibulose bis-phosphate(RuBP)(RuBP)

    10 of 12 G3P molecules10 of 12 G3P moleculesconverted into 6 RuBPconverted into 6 RuBPmoleculesmolecules

    2 of 12 G3P molecules used2 of 12 G3P molecules usedto synthesize 1 glucoseto synthesize 1 glucose ATP energy used for theseATP energy used for these

    reactionsreactions

    Chapter 734

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    Chapter 734The CThe C33

    Cycle ofCycle of

    CarbonCarbonFixationFixationoror

    DarkDarkReactionsReactionsororLight-Light-

    IndependeIndependentntReactionsReactions

    oror

    Chapter 735Relationship BetweenRelationship Between

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    Chapter 735

    Relationship BetweenRelationship BetweenReactionsReactions

    The photo part ofThe photo part ofphotosynthesis refers tophotosynthesis refers to

    the capture of light energythe capture of light energy(light dependent(light dependentreactions)reactions)

    The synthesis part ofThe synthesis part ofphotosynthesis refers tophotosynthesis refers toglucose synthesis (light-glucose synthesis (light-

    inindependent reactions)dependent reactions)

    Chapter 736Relationship BetweenRelationship Between

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    Chapter 736

    Relationship BetweenRelationship BetweenReactionsReactions

    Light dependent reactionsLight dependent reactionsproduce ATP and NADPHproduce ATP and NADPH

    which is used to drivewhich is used to drivelight-independentlight-independentreactionsreactions

    Depleted carriers (ADP andDepleted carriers (ADP andNADPNADP++) return to light-) return to light-dependent reactions fordependent reactions for

    rechargingrecharging

    Chapter 737A Summary ofA Summary of

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    Chapter 737

    A Summary ofA Summary ofPhotosynthesisPhotosynthesis

    Light-Light-

    dependentdependentreactionsreactionsoccur inoccur in

    thylakoidsthylakoids

    Light-Light-independeindependentnt

    reactionsreactions(c(c33 cycle)cycle)

    occur inoccur instromastroma

    Chapter 738atera er 22 an t ean e 44

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    Chapter 738

    ater,a er, 22, an t e, an e 44PathwayPathwayThe ideal leaf:The ideal leaf:

    has large surface area tohas large surface area tointercept sunlightintercept sunlight

    are porous to allow for COare porous to allow for CO22

    entry from airentry from airProblem: Substantial leafProblem: Substantial leafporosity leads to substantialporosity leads to substantial

    water evaporation, causingwater evaporation, causingdehydration stress on the plantdehydration stress on the plant

    Plants evolved waterproofPlants evolved waterproofcoating and adjustable porescoating and adjustable pores(stomata) for CO(stomata) for CO2 entrentry

    Chapter 739

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    Chapter 739

    When Stomata Are CloseWhen Stomata Are CloseWhen stomata close, COWhen stomata close, CO22 levelslevels

    drop and Odrop and O22 levels riselevels riseCarbon fixing enzyme combines OCarbon fixing enzyme combines O22instead of COinstead of CO22 with RuBP (calledwith RuBP (called

    photorespirationphotorespiration))Photorespiration:Photorespiration:OO22 is used up as COis used up as CO22 isisgeneratedgenerated

    No useful cellular energy madeNo useful cellular energy madeNo glucose producedNo glucose producedPhotorespiration isPhotorespiration isun roductive and wastefulunproductive and wasteful

    Chapter 740

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    Chapter 740

    When Stomata Are ClosedWhen Stomata Are Closed

    Hot, dry weather causesHot, dry weather causesstomata to stay closedstomata to stay closed

    Oxygen levels rise as carbonOxygen levels rise as carbondioxide levels fall inside leafdioxide levels fall inside leaf

    Photorespiration veryPhotorespiration very

    common under suchcommon under suchconditionsconditions

    Plants may die from lack ofPlants may die from lack of

    glucose synthesisglucose synthesis Chapter 74144 ants e ucean s e uce

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    Chapter 741

    44 ants e ucean s e ucePhotorespirationPhotorespiration

    CC44 plants have chloroplastsplants have chloroplasts

    in bundle sheath cells as wellin bundle sheath cells as well

    as mesophyll cellsas mesophyll cellsBundle sheath cellsBundle sheath cellssurround vascular bundlessurround vascular bundles

    deep within mesophylldeep within mesophyllCC33 plants lack bundleplants lack bundle

    sheath cell chloroplastssheath cell chloroplasts

    Chapter 742

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    Chapter 742CC33 Plants & the PathwayPlants & the Pathway

    CC33

    plants useplants usethe Cthe C33 pathwaypathway

    In a CIn a C33 plant,plant,mostmost

    chloroplasts arechloroplasts are

    in mesophyllin mesophyll

    Chapter 743

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    Chapter 743CC33 Plants & the PathwayPlants & the Pathway

    MuchMuchphotorespiratiophotorespiration occurs undern occurs under

    hot, dryhot, dryconditionsconditions

    Chapter 744CC44 Plants & the CPlants & the C44

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    Chapter 744

    CC44 Plants & the CPlants & the C44PathwayPathway

    CC44

    plants useplants usethe Cthe C44 pathwaypathway

    In a CIn a C44 plant,plant,both mesophyllboth mesophyll& bundle-sheath& bundle-sheath

    cells containcells contain

    Chapter 745COCO ii

    Mesophyll cell in a CMesophyll cell in a C44 plantplant

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    Chapter 745

    CC44 PlantsPlants& the C& the C44PathwayPathway

    Almost noAlmost nophotorespiratiphotorespirati

    on occurson occursunder hot, dryunder hot, dry

    conditions.conditions.

    COCO22 isiscapturedcaptured

    with a highlywith a highly

    specificspecificenzyme.enzyme.

    MuchMuchglucoseglucose

    synthesissynthesis

    occurs.occurs.

    p yp y pp

    Bundle-sheath cell in a CBundle-sheath cell in a C44

    Ch tCh t 7

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    ChapterChapter 7

    The endThe end