Antioxidant and immonomodulatory activities of polysaccharides isolated from

Taxillus Chinensis and Uncaria rhyncophylla

Lin Zhang, Narsimha Reddy, Sundar Rao Koyyalamudi and Sang Chul Jeong

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Cancer

According to the World Health Organisation (WHO), cancer

killed more than 11.5 millions people around the world in 2030

Treatment

Western Medicine is Expensive - Not accessible for majority

Traditional Medicine is cost effective

- More research needed

BackgroundBackground

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Botanical Polysaccharides

Non-toxicity

Minimal Side- effects

Immunomodulatory properties

Anti-tumour actitive

BackgroundBackground

Joeng et al., 2012 J. Med. Food, 15, 58-65. Zhang et al., 2012, Int. J. Biol. Maromol (In press)

Aim and objectiveAim and objective

Aim :

Isolation and measurement of immunomodulatory activities of

polysaccharides from Uncaria rhyncophylla and Taxillus chinensis

Objectives :

Factionation of bioactive polysaccharides from U. rhyncophylla and T.

chinensis using gel filtration column

Screening these fractions for their immunomodulatory effects

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MethodologyMethodology

Hot water extraction (autoclaving) (121oC, 2h)

EtOH precipitation (95%) (Supernatant:EtOH =1:4 /24h) Centrifugation (10,447xg / 20min)Centrifugation (10,447xg / 20min)

Gel chromatography Sepharose CL-6BPhenol-sulfuric method

Medicinal plant

Supernatant

Precipitate

Crude polysaccharides

Different fractions

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Joeng et al., 2012 J. Med. Food, 15, 58-65.

Results of fractionation of polysaccharideResults of fractionation of polysaccharide

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Uncaria Taxillus

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The polysaccharide fractions of Taxillus chinensis:

• TCP-1

• TCP-2

• TCP-3

The polysaccharide fractions of Uncaria rhyncophylla:

• UC-1

• UC-2

Chemical composition analysis

- Analysis of mono-saccharides

- Mainly contains Glucose, Fructose, Xylose, Arabinose and Rhamnose

Preliminary characterisationPreliminary characterisation

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Immunomodulatory and Antioxidant Immunomodulatory and Antioxidant activitiesactivities

- Antioxidant assays

-DPPH- free radical test

-Yeast oxidative stress assay

- Immunomodulatory properties

- NO production

- TNF-α production

- Cell viability

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Results: Antioxidant activityResults: Antioxidant activity

           

      % Yeast cell growth (Water extracts) *

S.NO

(1mg/ml)

Molecular Mass

(kDa)

DPPH scavenging activity

of water extracts (Ascorbate

equivalent µM) # CHP (150 µM) H2O2 (2mM) LAH (75 µM)

TCP-1 541 207 ± 4 0 27.79 ± 2.11 23.02 ± 1.94

TCP-2 18 447 ± 0 30 ± 3.54 39.7 ± 2.11 25.09 ± 1.46

TCP-3 2 662 ± 0 37.5 ± 0 48.21 ± 2.11 32.65 ± 3.89

UC-1 524 245 ± 0 45 ± 3.54 41.17 ± 4.21 22.68 ± 0.49

UC-2  3 305 ± 0 77.5 ± 7.07 70.52 ± 2.11 25.09 ± 0.49

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Results: Immunomodulatory properties (T. chinensis)Results: Immunomodulatory properties (T. chinensis)

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Results: Immunomodulatory properties Results: Immunomodulatory properties

(U. rhynchophylla)(U. rhynchophylla)

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Results of cell viabilitiesResults of cell viabilities

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ConclusionConclusion

Polysaccharides of T. chinensis and U. rhyncophylla displayed high

immunomodulatory activities

Huge potential of these polysaccharides as natural anti-tumor agents

Further studies

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Schepetkin, I.A. & Quinn, M.T. Botanical polysaccharides: macrophage immunomodulation and therapeutic potential, Immunopharmacology 6 (2006) 317-333.

Jeong, S.C., Koyyalamudi, S.R., Jeong, Y.T., Song, C.H., & Pang, G. (2012). Macrophage Immunomodulating and Antitumor Activities of Polysaccharides Isolated from Agaricus bisporus White Button Mushrooms, Journal of Medicinal Food, 15, 58-65.

Sun, L., Peng, X., Sun, P., Shi, J., Yuan, X., Zhu, J., Tai, G., & Zhou, Y. (2012). Structural characterization and immunostimulatory activity of a novel linear α-(1→6)-D-glucan isolated from Panax ginseng CA Meyer. Glycoconjugate Journal, 1-8.

Jeong, S.C., Jeong, Y.T., Yang, B.K., Islam, R., Koyyalamudi, S.R., Pang, G., Cho, K.Y., & Song, C.H. (2010). White button mushroom (Agaricus bisporus) lowers blood glucose and cholesterol levels in diabetic and hypercholesterolemic rats, Nutrition Research, 30, 49-56

References References

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First and foremost, I would like to thank you my supervisor, Dr.

Smith, Dr.Reddy and Dr.Rao for their valuable guidance and advice.

I also thank Dr. Jeong for helping me in biological experiments.

The generous support of technical staff is also acknowledged.

AcknowledgementsAcknowledgements

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