Annual Report 2010 - biotec.or.th...Annual report 2010 National Center for Genetic Engineering and Biotechnology (BIOTEC) / National Center for Genetic Engineering and Biotechnology

This report was prepared according to the 2010 financial year of the Royal Thai Government, from 1 October 2009 - 30 September 2010.

Annual Report 2010National Center for Genetic Engineering and Biotechnology (BIOTEC)

Annual Report 2010

National Center for Genetic Engineering and Biotechnology (BIOTEC)

ISBN 978-616-12-0125-8

First Edition January 2011

Number of copies printed 500

Copyright © 2011 by


National Science and Technology Development Agency (NSTDA), Thailand

Thailand. National Center for Genetic Engineering and Biotechnology.

Annual report 2010 National Center for Genetic Engineering and Biotechnology (BIOTEC) /

National Center for Genetic Engineering and Biotechnology. Pathumthani : National Center for Genetic

Engineering and Biotechnology, National Science and Technology Development Agency, 2011.

p. : ill.

ISBN: 978-616-12-0125-8

1. Thailand. National Center for Genetic Engineering and Biotechnology – Annual report.

2. Biotechnology. 3. Genetic engineering. I. Annual report (National Center for Genetic Engineering and

Biotechnology)

354.593

Published by


National Science and Technology Development Agency (NSTDA)

113 Thailand Science Park

Phahonyothin Road, Klong 1, Klong Luang

Pathumthani 12120, Thailand

Tel: (66-2) 5646700

Fax: (66-2) 5646701-5

Website: http://www.biotec.or.th

CONTENTS

Appendices 71 List of Publications List of Patents and Petty Patents Honors and Awards Executives and Management Team

Message from the BIOTEC Executive Director 5

Facts and Figures 7

Research and Development 15

Commercialization and Private Sector Partnership 37

Human Resource Development 45

Public Awareness 53

International Collaboration 57

Impact of BIOTEC’s Output 65

MESSAGE FROM THE BIOTEC EXECUTIVE DIRECTOR

2010 has proved to be a memorable year for BIOTEC and NSTDA. In June 2010, NSTDA bid farewell to Dr. Sakarindr Bhumiratana, who provided strong leadership as NSTDA President for the past 6 years. Dr. Sakarindr led BIOTEC as the Executive Director before taking up the office of NSTDA President. Under the new leadership of Dr. Thaweesak Koanantakool, NSTDA will continue to position itself as a key partner for strengthening a knowledge-based society through science and technology.

This year, a synthetic antifolate compound P218 targeting the dihydrofilate reductase enzyme of the parasite P. falciparum and its drug resistant strains entered pre-clinical trials sponsored by Medicines for Malaria Venture (MMV). The design and synthesis of P218 are the results of research efforts from the team at BIOTEC led by Prof. Yongyuth Yuthavong. This is the first time that a drug-like compound from any Thai research group was selected to enter preclinical safety testing. This has proven that Thailand has the capability of moving beyond basic research phase to the next step in drug discovery.

During the end of the rainy season of this year, Thailand faced an unexpected flood affecting vast areas from the Northeast to the central part of the country. Rice farms were severely damaged due to the rapid increase in the high water level and the length of inundation by accumulated water due to poor drainage. BIOTEC has seen the positive results of a new strain of rice, Homcholasit with submergence tolerance, generated via the marker assisted breeding program headed by Dr. Theerayut Toojinda in collaboration with the Rice Department and Kasetsart University, which was being tested in the field at the time of the floods. This new rice strain can be completely submerged under water for approximately 20 days without losing its vitality. Currently, more seeds are being distributed to farmers to propagate in preparation for next year’s planting season.

As usual, throughout 2010 we welcomed researchers and students from different countries to be part of the BIOTEC research community. Besides enjoying the participation of researchers from the neighboring countries under our HRD Program for Asia Pacific, and returning students from Nanyang Polytechnic (Singapore), Temasek Polytechnic (Singapore), Atma Jaya Catholic University of Indonesia (Indonesia), BIOTEC also welcomed new comers from the University of Liverpool to spend 6 weeks as internees in BIOTEC labs.

Located at Thailand Science Park, BIOTEC shares the park with private sector tenants providing endless opportunity for our scientists to work side by side with companies. This year, we welcomed the opening of Virbac Animal Health-Biotechnology R&D Center in Asia located in BIOTEC’s incubator wing, and the launching of “KEEEN”, a bioremediation agent, developed by BIOTEC research team in collaboration with Hi-Grimm Environmental and Research Co. Ltd. The product is a result of a two-year collaborative research project, starting in November 2008, between BIOTEC and Hi-Grimm on selecting oil-degrading bacteria found in Thailand and developing culturing methods and media suitable for a variety of products.

With our excellent team and strong alliances, BIOTEC hopes to advance science and technology to fulfill our commitment to building a stronger society and enhancing the competitiveness of the country.

Dr. Kanyawim Kirtikara Executive Director, BIOTEC

FACTS AND FIGURES

BIOTEC was first set up under the Ministry of Science, Technology and Energy on 20 September 1983. After the establishment of the National Science and Technology Development Agency (NSTDA) on 30 December 1991, BIOTEC became one of the NSTDA centers, operating outside the normal framework of civil service and state enterprises. This enabled the Center to operate more effectively to support and transfer technology for the development of industry, agriculture, natural resources, environment and consequently the social and economic well-being of Thai people. Other centers under the NSTDA family include National Metal and Materials Technology Center (MTEC), National Electronics and Computer Technology Center (NECTEC), National Nanotechnology Center (NANOTEC) and Technology Management Center (TMC).

As a premier research institute in Thailand and Asia, BIOTEC operates research units located at Thailand Science Park and specialized laboratories hosted by various universities, covering a wide spectrum of research topics from agricultural science to biomedical science and environmental science. Apart from research laboratories, BIOTEC activities also include policy research, an outreach program, training and international relations.

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ORGANIZATION

BIOTEC

• Planning Division• Evaluation & Monitoring Unit• Budgeting Section• Policy Study and

Biosafety Unit• Rural Development

Technology Service Unit

• Intelligence Unit• Management Information Systems • Public Relations Section• International Cooperation Division• Organization Development Section• Facility Management

Section• Procurement Section

HRD and Platform Technology

Management Division• Training Unit• Platform Technology Management

AdministrationResearch Units

• Research Units located at Thailand Science Park

• BIOTEC’s Satellite Units located at universities and other government departments

Business Unit

• Development Units• Business Development and

Technology Transfer

National Center for Genetic Engineering and Biotechnology (BIOTEC) 9

RESEARCH UNITS at Thailand Science Park

BIOTEC Central Research Unit

Protein-Ligand Engineering and Mo-lecular Biology Research Program• Protein-Ligand Engineering

and Molecular Biology Laboratory • Virology and Cell Technology

Laboratory • Anti-Tuberculous Drug Research

Laboratory

Agricultural Biotechnology Research Program• Food Biotechnology Laboratory • Monoclonal Antibody Production

Laboratory • Animal Physiology Laboratory • Aquatic Molecular Genetics and

Biotechnology Laboratory • Shrimp-Virus Interaction Laboratory• Microarray Laboratory • Starch Biosynthesis Laboratory • Plant Physiology & Biochemistry

Laboratory • Plant Molecular Genetics Laboratory • Plant Research Group

Bioresources Technology Unit

Bioresources Management Program• BIOTEC Culture Collection Laboratory • Biotechnology Law

Discovery Program• Bioresources Research Laboratory• Bioassay Laboratory• Enzyme Technology Laboratory• Fermentation and

Biochemical Engineering Laboratory• Microbial Engineering Laboratory

Microorganism Program• Mycology Laboratory • Mushroom Cultivation Research

Laboratory• Phylogenetics Laboratory

Information System Program• Information Systems Laboratory• Geoinformatics Laboratory• Ecology Laboratory

Genome Institute

• Genomic Research Laboratory• Proteomics Laboratory • Biostatistics and Informatics

Laboratory

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RESEARCH UNITS at universities and government organization

• Biochemical Engineering and Pilot Plant Research and Development Unit (BEC) - at King Mongkut’s University of Technology Thonburi (KMUTT)

• Excellent Center of Waste Utilization and Management (ECoWaste) - at King Mongkut’s University of Technology Thonburi (KMUTT)

• Cassava and Starch Technology Research Unit - at Kasetsart University

• Rice Gene Discovery Unit - at Kasetsart University

• Medical Biotechnology Research Unit - at Siriraj Hospital

• Biomedical Technology Research Center - at Chiang Mai University

• Center of Excellence for Marine Biotechnology - at Chulalongkorn University

• Center of Excellence for Molecular Biology and Genomics of Shrimp - at Chulalongkorn University

• Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) - at Mahidol University

• Peat Swamp and Rainforest Research Station - in Narathiwat Province

DEVELOPMENT UNITS

• Shrimp Biotechnology Business Unit (SBBU)• Dairy Cattle Production Research and Business Development Project• Shrimp Genetic Improvement Center (SGIC)• Pilot Plant for Nuclear Polyhedrosis Virus (NPV) Production


INCOME 1,111 million Baht

622 MBBudget allocated from NSTDA

84 MBFunding and income earned from joint / contract research and technical services

405 MBTonkla Archeep Program (a government economy stimulus program)

EXPENDITURE 1,017 million Baht

12 MB (1%)Technology Transfer

36 MB (4%)Infrastructure Development and Maintenance

123 MB (12%)Internal Management

409 MB (40%)Human Resource Development

437 MB (43%)Research and Development

1 MB (0%)Underprivileged

8 MB (2%)Energy

42 MB (10%) R&D Management

44 MB (10%)Platform technology

55 MB (13%)Health and Medicine

124 MB (28%) Environment

163 MB (37%) Agriculture and Food

Research and Development Expenditure 437 million Baht


HUMAN RESOURCES 544 employees

BIOTEC Staff by Education

32 (6%)Below B.S.

155 (29%)PhD

165 (30%)B.Sc.

192 (35%)M.Sc.

BIOTEC Staff by Job Function

8 (1%)Executive

127 (23%)Administrative

409 (76%)R&D and Technical Staff

PUBLICATIONS 216 papers

4In journals with impact factors more than 10

9In journals with impact factors between 4 and 10

18In non-citation index journals

185In journals with impact factors between 0 and 4


HONORS AND AWARDS 26 awards

PATENTS

Awarded

2Petty patent

3Patent overseas

4Patent in Thailand

Filed

14Patent

20Petty patent

11International awards

15Local awards



RESEARCH AND DEVELOPMENT

BIOTEC’s R&D and technical program covers a wide spectrum of research topics from agricultural to biomedical sciences and from energy to environmental science. In addition to applied research, the Center also focuses on building up the nation’s capacity in platform technologies.


HIGHLIGHTS ON RICE BIOTECHNOLOGY

Improvement of Rice Quality through Marker-assisted Selection Technology Rice has enormous economic value for Thai people. There are three ecosystems for rice production in Thailand including rain-fed lowland, irrigated lowland and upland. In rain-fed lowland, two famous varieties namely RD6, a glutinous jasmine rice and the non-glutinous jasmine rice Khao Dawk Mali 105 known as KDML105 are popularly grown by

farmers and are mainly intended for consumption and commercial purposes. Although these varieties are highly adapted to the rain-fed lowland environments, various constraints threaten their productivity by decreasing yield and consequently affecting the livelihood of farmers in the area. Problems in rice production include damage from diseases and insects and stress from floods, drought and soil salinity.


The Rice Gene Discovery Unit, a specialized unit established by BIOTEC and Kasetsart University, has long had research collaboration with the Bureau of Rice Research and Development to improve KDML105 and RD6 against adverse conditions. Marker-assisted selection (MAS) was employed in developing lines to hasten the transfer of gene/QTL more efficiently.

Three newly developed varieties are being field tested at the Rice Gene Discovery Unit in 2010. They are RD6 with 4 blast resistance genes pyramiding, non glutinous rice varieties with blight resistance and non-photoperiod sensitivity, and non glutinous rice varieties with brown planthopper resistance and non-photoperiod sensitivity. Three rice varieties; KDML105 with submergence tolerance and resistance to blight and brown planthopper, KDML105 with tolerance to drought and salinity, and Surin 1 with drought tolerance; are undergoing intensive field trials at the Rice Department, where they are being tested at various rice research stations and farmer fields across the country. One variety, KDML105 with submergence tolerance, is being reviewed for varietal registration. Two varieties, Homcholasit with

submergence tolerance and non-photoperiod sensitivity and RD6 with blast resistance, are in the process of applying for varietal registration.

In February 2010, NSTDA and the Rice Department signed an MOU to extend R&D collaboration into the next five years until 2015, aiming to develop additional varieties with desirable traits.


HIGHLIGHTS ON PLANT BIOTECHNOLOGY

of an efficient and rapid regeneration system via somatic embryogenesis, aiming for commercial planting of oil palm elite genotypes as well as genetic transformation studies. The study established a simple and rapid procedure to improve commercial production of clonal plantlets, using 13-week-old zygotic embryos as explants to culture on N6 medium with 2,4-D for callus induction. Somatic embryos are produced on maturation medium (N6 plus 2,4-D, puterscine, casein amino acids and activated charcoal) for 3-5 months. In plant regeneration, modified N6 medium with

Rapid Regeneration of Oil Palm Cultivation of oil palm has expanded enormously in recent years, due to the surge in demand for cooking oil and biofuel. Since oil palm is a perennial crop species with a long breeding cycle and a single growing apex, somatic embryogenesis has become the key method for multiplication of oil palm elite genotypes. In the study conducted by the Genome Institute and Kasetsart University, researchers evaluated the effect of medium composition and growth regulators on embryogenic competence and plant regeneration for the development


activated charcoal was used without growth regulators, whereby both shoots and roots were induced simultaneously. This combined shoot and root induction protocol shortened the culture time to 9-12 months instead of 18-24 months in normal protocol. The procedure will be extremely useful for commercial planting of oil palm elite genotypes as well as genetic transformation studies. Results of this study were published in Acta Physiologiae Plantarum.

Genome Sequence of Mungbean Mungbean, Vigna radiata, is an economically important crop grown principally for its protein-rich dry seeds. Mungbean is widely used as an ingredient in Asian cuisine, in the form of seeds or bean sprouts. The extracted starch is used to make transparent cellophane noodles. Despite its economic importance, genomic research of mungbean has lagged behind other species in the Fabaceae family. The research team at the Genome Institute, in collaboration with Kasetsart University, performed shotgun genome sequencing of V. radiata using the 454 pyrosequencing technology in order to obtain more genomic information. 470,024 shotgun sequences were assembled to 46,646 contigs. Around 1,500 microsatellites were identified that could be used as potential DNA markers and 192 loci were validated and demonstrated to be useful in Fabaceae. In addition, the researchers also observed that around 5% of the shotgun reads were derived from the mungbean chloroplast genome. The complete chloroplast genome sequence was obtained and characterized. These two major works were reported in DNA Research and BMC Plant Biology.

Genetic Linkage Map of Cassava Use of molecular technology for genetic improvement of cassava (Manihot esculenta) has been limited by the lack of a large

set of DNA markers and a genetic map. Researchers from the Genome Institute, Mahidol University and the Department of Agriculture developed additional simple sequence repeat (SSR) markers from the expressed sequence tags (ESTs), and constructed a genetic linkage map. In the study, researchers designed 425 EST-SSR markers from sequences obtained from the cassava EST database in GenBank, and integrated them with 667 SSR markers from microsatellite- enriched genomic sequences received from the International Center for Tropical Agriculture (CIAT). Of these, 107 EST-SSR and 500 genomic SSR primer pairs showed polymorphic patterns when screened in two cassava varieties, Huay Bong 60 and Hanatee, which were used as female and male parental lines, respectively. Within the 107 and 500 primer pairs, 81 and 226 EST-SSR and SSR primer pairs were successfully genotyped with 100 samples of F1 progeny, respectively. The results showed 20 linkage groups consisting of 211 markers—56 EST-SSR and 155 SSR markers—spanning 1,178 cM, with an average distance between markers of 5.6 cM and about 11 markers per linkage group. These novel EST-SSR markers provided genic PCR-based co-dominant markers that were useful, reliable and economical. The EST-SSRs were used together with SSR markers to construct the cassava genetic linkage map which will be useful for the identification of quantitative trait loci controlling the traits of interest in cassava breeding programs. This work was published in Molecular Breeding.


Application of High Resolution Melt (HRM) Analysis for Duplex Detection of Macrobrachium rosenbergii nodavirus (MrNV) and Extra Small Virus (XSV) in Shrimp Rapid and sensitive detection of pathogens is the key practice for prevention of disease outbreaks in shrimp farming. BIOTEC researchers at the Center of Excellence for Shrimp Molecular Biology and Biotechnology (Centex Shrimp) combined a multiplex RT-PCR with high resolution melt (HRM) analysis for the simultaneous detection of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) infection in the giant freshwater prawn, M. rosenbergii, as an example for the application of HRM to detect pathogens in shrimp. The newly developed technique is probe-free and does not require gel electrophoresis, thus speeding up the detection time and cost effectiveness. In

HIGHLIGHTS ON SHRIMP BIOTECHNOLOGY

addition, it is more sensitive than the traditional nested RT-PCR. This first application of HRM multiplex RT-PCR provides new potential for rapid and sensitive detection of multiple pathogens in shrimp. The work was published in Molecular and Cellular Probes.

Successful Propagation of Shrimp Yellow Head Virus in Immortal Mosquito Cells Research on crustacean viruses is hampered by the lack of continuous cell lines. A research team at Centex Shrimp partially solved the problem by challenging the C6/36 mosquito cell line with shrimp yellow head virus (YHV) and proved that YHV can be propagated successfully in C6/36 mosquito cells and could be used at low passage numbers as a source of inoculum to initiate lethal infections in shrimp. This leads to further studies that require the cultivation of YHV. The results of this work were reported in Diseases of Aquatic Organisms.

A Clip Domain Serine Proteinase Plays a Role in Antibacterial Defense in Shrimp Shrimp lack an adaptive immune response but rely on an innate immunity to protect themselves from surrounding pathogens. This defense system is able to eliminate the invading pathogens efficiently after infection. Antimicrobial peptides (AMPs) are important components of the innate immune system and function as a first line of defense against invading microorganisms by killing or slowing the growth of microbes like bacteria, fungi, viruses and protozoa. BIOTEC researchers at the Center of Excellence for Molecular Biology and Genomics of Shrimp identified the clip domain serine proteinases (Pmclip-SP1) in the black tiger shrimp (Penaeus monodon). This gene was up-regulated at 3 hours and down-regulated at 6–48 hours following Vibrio harveyi infection. Suppression of the PmClipSP1 gene by injection of double-stranded RNA (dsRNA)


led to a significant increase in the number of viable bacteria in the hemolymph (approximately 2.4-fold) and in the mortality rate (59%) of V. harveyi-infected shrimp. The findings suggest that PmClipSP1 plays a role in the antibacterial defense mechanism of P. monodon shrimp. Results of this study were presented in Developmental and Comparative Immunology.

Molecular Characterization of Stress-related Biomarkers in the Giant Tiger Shrimp Penaeus monodon Natural and farming environments can be variable and unpredictable. Under intensive culture conditions, shrimp can be exposed to suboptimal environmental conditions, such as elevated water temperature, low dissolved oxygen and significant reductions in salinity levels causing significant stress. BIOTEC researchers isolated several stress-related genes including calreticulin (CRT) and heat shock proteins (Hsp21, Hsp70 and Hsp90). Under the heat treatment (i.e. 35 °C for 2.5 hours), the expression levels of Hsp21, Hsp70 and Hsp90 in hepatopancreas of juvenile shrimp were significantly increased for 3, 2, and 11-fold. In gills, more prominent effects of

heat stress on Hsp90 were observed (23-fold in 2.5 hours), while comparable increases on Hsp21 but no effect on Hsp70 of heat shock were found. Unlike Hsp genes, CRT in gills and hepatopancreas after temperature stress were not significantly different, but the expression profile of CRT in hemocytes was up-regulated approximately 25 fold after treatment at 35 °C for 3 hours. Recombinant CRT protein was successfully expressed in vitro and exhibited an ability to form a complex with recombinant Endoplasmic Reticulum protein 57 of P. monodon (rPmERp57). The information indicated that Hsps and CRT genes can be regarded as biomarkers for temperature stress responses in P. monodon. This work was published in Developmental and Comparative Immunology.

Molecular Cloning and Expression of Progestin membrane receptor component 1 (Pgmrc1) and Progesterone receptor-related protein p23 (Pm-p23) of the Black Tiger Shrimp Penaeus monodon Genetic improvement of P. monodon cannot be achieved without knowledge on the control of its reproductive maturation. However, reduced spawning potential and low degree of maturation of P. monodon in captivity crucially prohibit the development of effective domestication and selective breeding programs in this species. Knowledge on molecular mechanisms of steroid hormonal induction on oocyte development may lead to possible ways to effectively induce ovarian maturation in shrimp. BIOTEC researchers successfully identified PmPgmrc1 and Pm-p23 of P. monodon. These reproduction-related genes were differentially expressed during ovarian development of P. monodon. Recombinant PmPgmrc1 and Pm-p23 proteins and their polyclonal antibody were produced and their expression analysis suggested that these gene products seem to play an important role in ovarian development and may be used as a bioindicator for monitoring the progression of oocyte maturation of P. monodon. This work was published in General and Comparative Endocrinology.


Risk Assessment of Histamine in Fish Sauce The presence of high levels of histamine is detrimental to the quality and safety of food, particularly fish sauce. Since fish sauce is a staple seasoning ingredient in Thai cuisine, with a value of 6.6 billion Baht for domestic consumption and 1 billion Baht in exports, a joint study to assess the risk of histamine in fish sauce was launched at BIOTEC Food Biotechnology Laboratory, in collaboration with the Department of Fisheries and The National Bureau of Agricultural Commodity and Food Standards (ACFS), both under

HIGHLIGHTS ON FOOD SCIENCE, TECHNOLOGY AND BIOTECHNOLOGY

the Ministry of Agriculture and Cooperatives. The study concluded that applying the current standard of 200 ppm histamine concentration or alternatively a standard of 400 ppm does not greatly affect risk to consumers. The result of this study was used by the Delegation of Thailand to propose to the 30th Session of the Codex Committee on Fish and Fishery Products (CCFFP) taking place on 24 September – 4 October 2009 in Morocco for the adjustment in the histamine concentration in fish sauce to 400 ppm (40 mg histamine /100g of fish sauce). The proposal was endorsed by the Committee. This action can help the Thai fish sauce industry to overcome trade barriers previously caused by conservative standards.

Utilizing Microorganisms to Degrade Histamine in Fish Sauce As high levels of histamine pose a risk of food toxification, BIOTEC Food Biotechnology Laboratory in collaboration with Prince of Songkla University and Chulalongkorn University, developed the


use of halophilic archaea to degrade histamine in high salt-fermented fishery products, including fish sauce. In 2008, the team reported on a novel halophilic archaeon, namely Natrinema gari BCC 24369 that was isolated from salted anchovy. Later the study, published this year in Enzyme and Microbial Technology, revealed that this selected strain is capable of reducing histamine in high salt condition and its histamine-degrading activity is mediated through the intracellular histamine dehydrogenase. Due to the limitations of applying Nmn. gari BCC 24369 cells and its enzyme caused by the slow growth rate, low yield of enzyme, and the cost of cell or enzyme preparation, a study on the immobilization of whole cells to provide potential advantages over applications of free cells and enzyme systems was performed. The immobilized whole cells retained histamine-degrading activity as high as 94% of the original activity detected in free whole cells. Immobilized whole cells rendered stable histamine-degrading activity at high salt concentration

and temperature. This work was reported in Food Chemistry and could be applied directly in fish sauce. From these studies, a patent on “Biological process for degradation of histamine in high salt containing foods by using halophilic archaea producing histamine dehydrogenase” was filed in November 2009.

Screening for Starter Culture for Fermented Pork Sausage Nham is a Thai traditional fermented pork sausage made from minced pork, boiled pork rinds, cooked rice, garlic, salt, sugar, pepper, chili and sodium nitrite, packed in banana leaves or plastic sheets and allowed to ferment for approximately 3–4 days. Over-fermentation of Nham, resulting in a variety of undesired flavor and texture changes such as water dripping, discoloration and off-flavor development, can take place during handling, transportation or storage if not refrigerated. There is considerable interest in an economic solution to prevent over-fermentation. A mutated starter culture whose growth is sensitive to high acidity, i.e. pH 4.6, the desirable pH of Nham specified by Thailand Food Standards and apply them in Nham fermentation, can offer a strategy to overcome the over-fermentation problem.

In the study conducted jointly by BIOTEC Food Biotechnology Laboratory, Mahidol University and the Center of Excellence for Agricultural Biotechnology, several acid-sensitive mutants of one of the commercial Nham starter cultures, Lactobacillus plantarum BCC 9546, were isolated as spontaneous neomycin-resistant mutants. The most acid-sensitive mutant was applied in experimental Nham production and the pH of Nham fermented with the mutant was significantly higher at the end of fermentation (3 days) and after an additional 4 days of storage at 30 °C. These results indicate that the use of acid-sensitive L. plantarum as starter culture can reduce the severity of post-acidification and increase the shelf life of Nham at ambient temperature. The results of this study were published in Food Microbiology.


Microbial Utilization in Animal Feed Industry BIOTEC Culture Collection (BCC) holds more than 40,000 strains of microorganisms, consisting of 68% filamentous fungi, 23% bacteria, 8% yeast and 1% algae. Part of the work conducted by the Bioresources Technology Unit is to find application for these local bioresources in the animal feed industry. Four enzymes, namely mannanase, phytase, xylanase and cellulase

HIGHLIGHTS ON BIORESOURCES STUDY AND UTILIZATION

produced from in-house microbial collection are being investigated. Mannanase enzyme is being produced at pre-pilot scale for testing in a chicken farm, to study the potential use as a feed supplement. Thermostable recombinant phytase has been tested to be on par with commercially available phytase. Scale-up production of 200 L is being conducted, while the pre-pilot scale level (50L) has already been achieved. Xylanase and


cellulase from Thai-isolated fungi were tested and were shown to improve fiber digestion and consequently, to increase metabolizable energy in poultry. Testing with pigs is currently being planned with a commercial farm. Apart from work with enzymes, BIOTEC scientists also developed high-density cultivation technology for Schizochytrium production in a 300 L fermentor. With this technology, up to 24 g/l of DHA is obtained. The technology will likely be of interest to the aquaculture feed industry.

Enzyme Discovery for Ethanol Production from Bagasse Bagasse, the solid residue left after extraction of sugarcane juice, is one of the major lignocellulosic plant residues. With its high cellulosic polysaccharide content, it is considered as a potential source for ethanol production. Researchers from the Bioresources Technology Unit conducted experiments on enzymatic hydrolysis and fermentation of pre-treated bagasse, using enzyme-producing fungi and yeast strains available in the BIOTEC Culture Collection. The enzymatic hydrolysis stage utilizes an enzyme cocktail made up of crude enzymes prepared from Penicillium chrysogenum BCC4504 (containing cellulase activity) and Aspergillus flavus BCC7179 (containing complementary β-glucosidase activity); whilst the fermentation step uses Pichia stipitis. This study demonstrated the potential use of local microorganisms in the production of ethanol from this potent lignocellulosic biomass. The lab scale results of the experiment have been reported in the Journal of Bioscience and Bioengineering. The research team also developed a multi-enzyme, consisting of non-starch polysaccharide hydrolyzing enzyme and raw starch degrading amylolytic enzyme, prepared from local Aspergillus in the BIOTEC Culture Collection for cassava hydrolysis. These multi-enzymes can be utilized with various forms of cassava, such

as fresh roots, chips and pulp in the non-thermal hydrolysis and saccharification process. Studies on these multi-enzymes are underway for scale-up production and its effectiveness in various applications including biogas production and as an animal feed supplement.

Biocontrol Application To search for potential biocontrol agents, isolates maintained at BCC have been screened for their toxicity against the economically important insect pests. Beauveria bassiana BCC 2660 was evaluated for biological control of green peach aphid, Myzus persicae (Sulzer), in kale greenhouse. The performance is 4 times better than the commercial formulation. Currently, scientists are working in collaboration with the Rice Department and the Department of Agriculture to test its effectiveness in controlling brown plant hopper (Nilaparvata lugens) and Myzus persicae in the field. In the Bacillus thurigiensis study, scientists discovered the Vip3Aa protein to be highly effective on beet armyworm (Spodoptera exigua) and leafworm (Spodoptera litura), when compared to protein extracted from commercial Bt. Field testing of Vip3Aa protein is being planned with TFI Green Biotech Co., Ltd., a manufacturer of Bt for biocontrol.


Development of Effective Antifolate Drug For decades, the group at BIOTEC Protein-Ligand Engineering and Molecular Biology Laboratory have long been engaged in research towards developing

HIGHLIGHTS ON MALARIA RESEARCH

antimalarial drugs to overcome multi-drug resistant malaria. The main strategies revolve around rational drug design and the synthesis of new effective antimalarials based on the structures of the drug targets. The team


has discovered the protein structure of dihydrofolate reductase-thymidylate synthase of Plasmodium falciparum (PfDHFR-TS), an important drug target of Plasmodium parasites. Based on information obtained from co-crystal structure of DHFR and selected compounds, researchers were able to design and create better effective compounds against resistant parasites. Initial safety testing indicated that one of these compounds, P218, has a good safety margin between the toxicity in animals and the predicted effective human dose. The compound will be examined by another group for its parasite reduction ratio (PRR) value and propensity to generate new resistance before further decision by the Medicines for Malaria Venture (MMV).

Preclinical Evaluation of Antifolate QN254 Researchers in BIOTEC Protein-Ligand Engineering and Molecular Biology Laboratory took part in a preclinical evaluation of antifolate QN254 launched by Novartis Institute for Tropical Diseases and Wellcome Trust. BIOTEC researchers performed the inhibitory activity test of QN254 against pfDHFR and determined the crystal structure of compounds in PfDHFR-TS. The team provided biochemical and structural evidence that QN254 binds and inhibits the function of both the wild-type and the quadruple-mutant (V1S) forms of the DHFR enzyme. Study by other partners in the project found that the compound is highly active against Plasmodium falciparum clinical isolates, displaying various levels of antifolate drug sensitivity. The compound displayed favorable pharmacokinetic

properties after oral administration in rodents and was remarkably efficacious against Plasmodium berghei, leading to fully cured infected mice. In the course of these efficacy studies, however, some dose limiting toxicity was uncovered at higher doses. In conclusion, despite its relative in vitro and in vivo selectivity toward the Plasmodium DHFR enzyme and malaria parasites, QN254 does not show the adequate therapeutic index to justify its further development as a single agent. This work was reported in Antimicrobial Agents and Chemotherapy.


Role of Innate Immune Responses in Dengue Virus Infection Role of innate immune parameters in the course of dengue virus infection and pathogenesis of dengue is poorly understood. The main dilemma is due to the lack of satisfactory small animal models for dengue virus infection. The only animal species besides humans that are known to be naturally infected and that can be experimentally infected via the parenteral route are monkeys and apes. Previous studies using subcutaneous and/or intramuscular experimental inoculation of rhesus macaques with dengue virus demonstrated the antibody and viremia response in the pattern relatively similar to that in man. However, clinical sequelae typical of human dengue virus infection have never been observed in the previously existing monkey models. In a joint study between scientists

HIGHLIGHTS ON DENGUE RESEARCH

from the Medical Biotechnology Research Unit, a joint unit between BIOTEC and the Faculty of Medicine Siriraj Hospital, and Emory University, it was for the first time found that rhesus macaques inoculated intravenously with a high dose of dengue virus serotype 2 (strain 16681) produced dengue hemorrhage, one of the key clinical manifestations detected in dengue patients. The established monkey model may therefore provide a unique platform to define the early events in dengue virus infection and help identify which blood components contribute to the pathogenesis of dengue disease. This work was published in Blood.

A Re-evaluation of the Mechanisms Leading to Dengue Hemorrhagic Fever Viremia is one of the important features of dengue virus infection among the flaviviruses. Dengue virus infection results in a spectrum of clinical symptoms, ranging from undifferentiated flu-like illness, mild dengue fever, to dengue hemorrhagic fever (DHF)/dengue shock syndrome (DSS), a life-threatening illness. Several mechanisms have been hypothesized based primarily on data collected from post-acute clinical phase to account for DHF/DSS. Among all the hypothesized mechanisms that have been forwarded to date concerning the etiology of DHF, the understanding of the dynamics of dengue viremia is perhaps the most critical since this can potentially influence vaccine design to prevent dengue virus infection and dengue disease development. Scientists from the Medical Biotechnology Research Unit and Emory University presented a novel alternative concept regarding how dengue virus disseminates during the acute viremia period based on its intimate relationship with platelets. They demonstrated that dengue viral particles are present within platelets isolated from dengue patients and that dengue viral products could be detected within platelets experimentally infected


in vitro. Their results suggested that platelets may be involved in the initial replication and dissemination of dengue virus in infected humans and may shield dengue virus from the host immune system. This work, therefore, provides a useful piece of information regarding the status of dengue virus and its potential survival strategy in circulation during acute infection. The study was reported in Annals of the New York Academy of Sciences.

Cross-Reacting Antibodies Enhance Dengue Virus Infection in Humans In a collaborative research between Imperial College London, Faculty of Medicine Siriraj Hospital Mahidol University, Khon Kaen Hospital, Songkhla Hospital, and the Medical Biotechnology Unit, scientists have analysed a panel of dengue specific human monoclonal antibodies (hAb) from human B cells isolated from dengue infected patients. Antibodies to the structural precursor-membrane protein (prM) form a major component of the response. These antibodies are highly cross-reactive among the dengue virus serotypes and, even at high concentrations, do not neutralize infection but potently promote antibody dependent enhancement (ADE). ADE is the phenomena whereby non-neutralised serotype cross-reactive antibodies amplify the number of virus infected cells by binding to virions as immune complex which then be taken up into Fc receptor bearing cells, leading to more infected cells. ADE has been proposed as being one of the factors contributing to severe dengue disease (Dengue Hemorrhagic fever, DHF). Anti-prM hAbs can enhance virus infection in not only cell lines but also in primary cells such as monocytes and dendritic cells. Scientists propose that the partial cleavage of prM from the viral surface reduces the density of antigen available for viral neutralization, leaving dengue viruses susceptible to ADE by antibody to prM. This finding has implications for future vaccine design in which the dengue vaccine should not induce anti-prM Ab responses in order to avoid ADE. The work was published in Science.

Immunodominant T-cell Responses to Dengue Virus NS3 Are Associated with DHF In this study, scientists set out to study T-cell responses across the entire dengue virus proteome and to see whether these were related to disease severity in a cohort of dengue-infected children from Thailand. Robust responses were observed in most infected individuals

against most viral proteins. Responses to dengue non-structural protein 3 (NS3) were the most frequent, and there was a very strong association between the magnitude of the response and disease severity. Furthermore, in severe dengue cases (DHF cases) cytokine-high CD107a-negative cells predominated, suggesting that these cells may play a role in immunopathogenesis of the dengue disease. This work was published in the Proceedings of the National Academy of Sciences (PNAS).

Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles During Flavivirus replication, new virus progenies containing envelope (E) and prM glycoproteins on their surfaces are generated as non-infectious, immature virions. Cleavage of the prM proteins by host furin-like protease releases extracellular infectious, mature virions, containing E and embedded M proteins, from infected cells. However, an incomplete cleavage of the prM glycoprotein of dengue virus generates a mixture of mature (prM-less) and prM-containing, immature extracellular particles. In the collaborative research between Chiang Mai University, Purdue University, Faculty of Medicine Siriraj Hospital and BIOTEC, “Partially mature virion”, as a third type of extracellular particles, the major prM-containing particles in a dengue serotype 2 virus, was demonstrated by sequential immunoprecipitation and cryo-electron microscopy. Changes in the proportion of viral particles in the pr-M junction mutants exhibiting altered levels of prM cleavage suggest that the partially mature particles may represent an intermediate subpopulation in the virus maturation pathway. These findings are consistent with a model suggesting the progressive mode of prM cleavage. The results of this study were presented in the Journal of Virology.


Extraction of Neuraminidase of H5N1 Influenza Virus Using Thrombin Proteolytics BIOTEC researchers employed reverse genetics to construct non-pathogenic recombinant influenza A viruses, termed rgH1N1

LVPR

and rgH1N1LVPR-GS

, that harbor the neuraminidase (NA) of H5N1 virus engineered to contain a specific thrombin cleavage site at the stalk region. By using thrombin to cleave NA at its stalk, a productive extraction of NA globular heads could be obtained from purified rgH1N1

LVPR. Furthermore, it was found

HIGHLIGHTS ON INFLUENZA RESEARCH

that the NA of rgH1N1LVPR-GS

could be cleaved by endogenous thrombin present in embryonated chicken eggs, resulting in the release of NA globular heads into allantoic fluids. These data highlight the use of thrombin cleavage as an effective strategy for extraction of active NA heads directly from live viral particles not only of H5N1 but, theoretically, of any subtype of influenza A viruses. This work was published in the Journal of Virological Methods.


Prediction of Avian Influenza A Binding Preference to Human Receptor It is known that the highly pathogenic avian influenza A virus H5N1 binds strongly and with high specificity to the avian-type receptor by its hemagglutinin surface protein. This specificity is normally a barrier to viral transmission from birds to humans. However, strains may emerge with mutated hemagglutinin, potentially changing the receptor binding preference from avian to human-type. The change in binding preference is due to mutation, which can be computationally modelled. BIOTEC researchers in collaboration with Kasetsart University, used the information from the available structures of HA complexed with avian and human receptors to predict the HA binding selectivity from different HA variants in receptor-based conformational analysis by measuring a single torsion angle during molecular dynamic simulation and found the predictions of receptor preference agreeable with the available in vitro binding data. This work was published in BMC Genomics.


Human Genetic Mapping of Asia BIOTEC researchers were among 90 scientists participating in the Human Genome Organisation’s (HUGO’s) Pan-Asian SNP Consortium, engaging in the study to map genetic diversity in Asia, covering 73 Southeast Asian (SEA) and East Asian (EA) populations. This human genetic mapping of Asia has important implications, especially in the further understanding of migratory patterns in human history, and for the study of genetics and diseases. The findings were published online in a report in Science on 10 December 2009. In a genome-wide analysis of genetic variation using more than fifty thousand single nucleotide polymorphisms (SNPs)

HIGHLIGHTS ON BIOINFORMATICS AND INFORMATION SYSTEMS

typed in thirteen population samples from Thailand, it was found that Mlabri, a group of nomadic hunter-gatherers inhabiting the rural highlands of Thailand, share more recent common ancestry with the Htin. This can potentially be the first genetic evidence that supports the linguistic affinity of Mlabri, and this association between linguistic and genetic classifications could reflect the same past population processes. The study was published in BMC Genetics.

RExPrimer: An Integrated Primer Designing Tool Scientists at the Genome Institute have developed an integrated graphical web-based application for primer


design, called RExPrimer, which was written in Python language. RExPrimer provides many functionalities for improved PCR primer design. Several databases, namely annotated human SNP databases, insertion/deletion (indel) polymorphisms database, pseudogene database, and structural genomic variation databases were integrated into RExPrimer, enabling an effective without-leaving-the-website validation of the resulting primers. By incorporating these databases, the primers reported by RExPrimer avoid mis-priming to related sequences as well as possible PCR failure because of structural polymorphisms. In the study published in BMC Genomics, researchers demonstrated the program effectiveness in successfully generating primers for strong homologous sequences. This software is freely available at http://www4a.biotec.or.th/rexprimer.

ipPCA: A Novel Population Structure Analysis Algorithm Population structure analysis is important to genetic association studies and evolutionary investigations. As genotypic datasets become ever larger, population structure analysis becomes progressively difficult. Therefore, to keep abreast with the ever increasing size and complexity of genotypic data, BIOTEC researchers, in collaboration with researchers from NECTEC and the Faculty of Medicine Siriraj Hospital, developed a novel population structure analysis algorithm called iterative pruning PCA (ipPCA). In the study reported in BMC Bioinformatics, ipPCA performance was tested on real datasets and able to accurately resolve even highly structured populations

containing many closely related subpopulations. This software is freely available at http://www4a.biotec.or.th/GI/tools/ippca.

iCollect: An Enhanced Solution for Biological Resources Management iCollect is a software to support laboratory inventory management of biological collections at BIOTEC. iCollect is designed with a highly customizable, configurable and user-friendly interface to allow users to create and store samples; graphically view the content of samples and search information in a collection. Using the barcode assigned by iCollect, users can easily find a sample’s position in the storage, and add a sample to a selected storage position. The in and out movement of samples in the storage are recorded and tracked for audit trail. Moreover, the graphical tree view of the samples can be used to trace the aliquot/sample back to its original parent.


Biosafety Guidelines for Contained Use of Genetically Modified Microorganisms (GMM) at Pilot and Industrial Scale BIOTEC, as the Secretariat to the Technical Biosafety Committee (TBC), released the Biosafety Guidelines for Contained Use of Genetically Modified Microorganisms (GMM) at Pilot and Industrial Scale in November, 2010. The Guidelines provide an important framework for researchers and entrepreneurs to utilize genetically modified microorganisms in all industrial production such as food industries, agriculture industries and environmental industries etc. The document outlines the safe handling of GMM in pilot

HIGHLIGHTS ON RISK ASSESSMENT AND BIOSAFETY

and industrial scale, covering the subjects of the containment and facility management to protect its exposure to workers or environment.

OECD Consensus Document on Compositional Considerations for New Varieties of Papaya Since 1999, OECD’s Task Force for the Safety of Novel Foods and Feeds has focused its work on the development of science-based consensus documents, which are mutually acceptable among member countries. These consensus documents contain information for use during the regulatory assessment


of a particular food/feed product. In the area of food and feed safety, consensus documents are being published on the nutrients, anti-nutrients or toxicants, information of its use as a food/feed and other relevant information. Thailand took the lead, in collaboration with the United States (co-lead), to develop the consensus document addressing compositional considerations for new varieties of papaya (Carica papaya L.) by identifying the key food and feed nutrients, anti-nutrients, toxicants and allergens. A general description of these components is provided. As well, there is background

material on the production, processing and uses of papaya and considerations to be taken into account when assessing new varieties of papaya. Constituents to be analysed, related to food use and to feed use, are suggested. The Document was endorsed by The Task Force and released for public access on the OECD website in June 2010. Among plants and crops included in the previous consensus documents were canola, soybean, sugar beet and potato. Thailand is the first non-OECD member country to lead the development of a consensus document.

BIOTEC places strong emphasis on promoting the industrial applications of biotechnology in both Thai and foreign companies. Technology/product licensing as well as collaborative research with the private sector are among mechanisms to promote bio-business in Thailand. Recognizing that the majority of companies in Thailand are of small and medium size and do not have in-house R&D capability, BIOTEC also provides R&D services in the form of contract research.

COMMERCIALIZATION AND PRIVATE SECTOR PARTNERSHIP


LICENSING AGREEMENTS

During FY 2010, six licensing agreements were accomplished with the following clients.

SHRIMP BIOTECHNOLOGY BUSINESS UNIT (SBBU) SBBU was granted the rights to manufacture and distribute Taura Syndrome Virus (TSV) and White Spot Syndrome Virus (WSSV) detection kits based on the LAMP-LFD technique. Compared to PCR-based methods, these test kits are economical, swift and simple to use.

MBSAsia Ltd. The licensing agreement covers the permission to distribute monoclonal antibodies for progesterone hormones. Progesterone levels can be

used to confirm that cows are in estrus phase before performing artificial insemination, as well as to confirm their pregnancy.

Innova Biotechnology Co., Ltd. Under the agreement, Innova Biotechnology has the rights to produce and distribute detection kits for white leaf disease in sugarcane. These kits can be used on-site to detect phytoplasma directly in molasses. They can be used as a risk management tool to reduce the spread of and damage from the white leaf disease.

New World Biotech Co., Ltd. New World Biotech acquired the rights to manufacture and distribute


Infectious Myonecrosis Virus (IMNV) detection kits based on the LAMP-LFD technique. They can be used to reduce and contain IMNV infection in shrimp aquaculture.

TFI Green Biotech Co., Ltd. TFI Green Biotech was granted the production rights on Bacillus megaterium for the purpose of evaluating its efficacy against Rhizoctonia solani, which causes rice sheath blight. B. megaterium has been formulated into stable, effective, easy-to-use and safe biopesticide.

Drew-Bio (Thailand) Co., Ltd. Drew-Bio (Thailand) obtained the rights to evaluate the performance of prototypical tools that can interpret types of Thalassemia, Thalassemia carriers, and abnormal hemoglobin from hemoglobin type result obtained from Low Pressure Liquid Chromatography (LPLC). These prototypes, together with mean corpuscular volume (MCV) information, can be added-on to any LPLC for automatic Thalassemia test result interpretation. This system uses computer and electronic technologies to intercept the print out signed from LPLC and analyze it together with MCV data to offer the Thalassemia type interpretation for physicians and medical technicians.


Several products and prototypes developed from BIOTEC laboratories have been introduced this year and some examples are:

iBrew Bovine Meat Quality Genotyping Kit iBrew can be used to predict the meat quality of beef cattle. The Kit works by determining the genotypes of four Single Nucleotide Polymorphisms (SNPs) in Calpastatin (CAST), Calpain (CAPN) and Thyroglobulin (TG) genes that influence meat quality traits, in terms of tenderness and juiciness, in cattle by using the restriction fragment length polymorphism (RFLP) method. iBrew uses non-toxic chemicals and is also user-friendly, giving results within 3 hours. The test will be useful in making the selection of sires and dams for breeding, as well as predicting the meat quality of calves and cattle.

LAB TO MARKET HIGHLIGHTS

Azupure Sperm DNA Purification Kit Azupure is a genomic DNA kit for extraction and purification from fresh or frozen semen and whole blood of mammals such as humans, canines and cattle. Employing a simplified and rapid centrifugation method for DNA extraction process, the Kit offers short preparation times and fast extraction of sperm DNA in approximately 2 hours, using non toxic chemical reagents. Azupure can be applied in forensic science, mainly in the examination of semen stains; medical science such as examining male infertility; as well as livestock breeding and improvement. It is suitable for on-farm use, as well as in a research laboratory. ENZstrips ENZstrips are test strips containing in-gel crosslink substrate for detecting cellulases, hemicellulases, beta-glucanases and amylases enzyme activity for


industrial and academic research use. To detect enzyme activities in the sample, the test strip, made of plastic polymer, is dipped in the sample solution or extract. Enzymes in the sample will specifically attack its chromogenic substrates immobilized in the gel attached to the strip. Reactions can be incubated under a wide range of conditions and would take from a few minutes to hours depending on enzyme activity level in samples. Once color is observed, it means that enzyme activity has taken place.

BIOTEC researchers are encouraged to participate in various activities implemented by NSTDA designed to support their “Lab to Market” strategy, such as bootcamps, early-stage technology commercialization plan competitions (I2P competition) and technology business plan competitions (Technopreneur competition). Through this incubation, researchers gain critical feedback and advice about the business model, management team, competitive positioning, market strategy and other key elements to adjust their technology to better address market opportunities. As a result of these programs, the following two BIOTEC inventions were featured at NSTDA Investors’ Day, a public event to introduce NSTDA’s products and inventions to potential investors:

Aqua-RASD Aqua-RASD is a closed recirculating aquaculture system integrated with hybrid nitrification biofilter tanks for ammonia removal and patented Tubular Denitrification Reactor (TDNR) for nitrate removal. With TDNR, water can be recirculated within the system hence water exchange can be prolonged for a year or more.

K9 Diagnostic Kit-MDR1 K9 Diagnostic Kit-MDR1 is a canine drug allergy test kit, using blood samples. The results can be obtained within an hour, making it suitable for animal clinics and hospitals, as well as for animal owners to perform the test at home.

This year, Hi-Grimm Environmental and Research Co. Ltd., launched a bioremediation product line under the brandname “KEEEN”. KEEEN is the result of a two-year collaborative research project, between BIOTEC and Hi-Grimm Environmental and Research Co. Ltd., for selecting oil-degrading bacteria found in Thailand and developing culturing methods and media suitable for a variety of products. KEEEN provides its services into seven lines of business to cater for the specific demands of its customers; 1. Solution Wastewater Treatment, 2. Solution Oil Tank Degassing/Cleaning Remediation, 3. Solution Oil Spill Response/Clean Up, 4. Solution Industrial Application, 5. Solution Site Contamination and Remediation Service, 6. Solution Sanitary Treatment and 7. Solution Testing/Diagnosis/Research.


COLLABORATIVE RESEARCH

BIOTEC conducted a total of 19 collaborative research projects - 10 of which are new projects during FY 2010.

ThanapaisalApplication of enzyme-mix in a single-step desizing of and lipid stain removal from cotton fabric

Thai Fermentation Industry Co., Ltd.Corynebacterium glutamicum strain improvement for effective L-glutamate production

Arysta LifeScience Corporation (Japan)Rearing and production of granulosis virus to control leaf roller caterpillars

PTT Chemical Public Company Limited (PTTCH)Development of gamma-linolenic acid production from Mucor circinelloides

Thai Better Foods Co., Ltd.Development of heat-humidity method for modified tapioca starch production at semi-industrial scale

Asian Aqua & Animal (Thailand) Co., Ltd.Bacillus sp. strain selection and screening for digestion of organic matter and anti-microbial properties to be used in shrimp aquaculture

BioMedCore Inc. (Japan)Evaluating mannose-coated liposome as shrimp immune booster

Mitr Phol Research and Development Center Co., Ltd.Sugarcane strain improvement for salinity stress-tolerance

Sun Feed Co., Ltd.Optimization of fungal beta-mannanase production and feasibility study of the enzyme for corn-based diet digestion

Pokphand Aquatech Co., Ltd.Development of loop mediated isothermal amplification combined with the lateral flow dipstick (LAMP-LFD) technique to detect yellow head virus in shrimp and carriers


CONTRACT RESEARCH AND CONSULTING SERVICES

In 2010 BIOTEC performed 28 contract research projects and 14 technical consulting projects. Of these 42 projects, 15 are new initiatives. Clustered by industry applications, 6 of the new projects were under the

agriculture and food sector, 5 were under the energy and environment sector, and the last 3 were under the health and medicine sector.



HUMAN RESOURCE DEVELOPMENT

BIOTEC places high priority on human resource development in terms of increasing the quantity and quality of human resources in this sector as well as upgrading and educating the workforce. Various activities are designed to capture different segments of the workforce as well as to address a variety of objectives, ranging from providing scholarships/ fellowships to organizing training workshops for academics and industries, to organizing youth programs.


During the year, BIOTEC organized 22 training workshops and seminars, as well as the 7th National Symposium on Marine Shrimp, altogether attended

TECHNICAL WORKSHOPS AND ACADEMIC SEMINARS

by 853 participants (1,858 man-days). Topics of workshops and seminars on offer covered various themes of biotechnology, e.g. plant biotechnology, animal biotechnology, biosafety and food safety and cutting-edge technologies.

Public seminars with local and overseas guest speakers are regularly organized with audiences from universities, companies and government agencies. Over 40 seminars were held in 2010.

Training Workshop on Application of Double Haploid Technique for Cucumber Improvement

Thai - Franco Seminar on Small RNA in Oil Palm Reproductive Development

Training Workshop on BITEME: BIoTEc Microarray Enterprise

Training Workshop on Biosafety Guidelines for Work Related to Modern Biotechnology or Genetic Engineering Training Workshop on

Biotechnology for Cattle Production

Training Workshop on Biotechnological Methods for Gynogenesis of Cucumber and Androgenesis of Pepper

Workshop on Ovulation Induction in Cattle

Workshop on Tissue Culture of Teak

Workshop on Proteomics Analysis and Application: Case Studies in Shrimp and Spirulina Research Projects

Workshop on Gene Discovery of Uncultured Microbes Using Metagenomic Approach

Workshops on Food Safety Risk Assessment Workshop on

Biotechnology Business and Regulation

Workshop on Screening Protocol for Root Knot Nematode, Meloidogyne spp

Workshop on Food Safety Risk Assessment

Workshop on Genetic Factors in Broodstock Management for Selective Breeding Program in Marine Shrimp

Workshop on Biosafety Policy and Framework

Workshop on Diagnostic Technology for Food-borne Pathogens and Chemical Contamination in Food Products


TONKLA ARCHEEP PROGRAM – GOVERNMENT ECONOMIC STIMULUS PACKAGE

In March 2009, the Royal Thai Government launched the Tonkla Archeep Program, as part of the government economic stimulus package to alleviate the nation’s economic setback. Tonkla Archeep Program aimed to provide vocational training for the workforce affected by economic downturn, allowing them to re-enter the job market or start operating their own small farming business with improved and new skills.

BIOTEC was one of many organizations selected to provide service to the Government in managing the project run from June 2009 – June 2010, with total funding of 517 million Baht. To undertake this

challenging and large-scale task, an alliance was formed with the Community Enterprise Institute and its network of over 1,000 training centers across the country to offer on-site training to the workforce in 53 provinces. The training offered was in the areas of small and micro community enterprise and holistic approach to sufficiency economy. Over 50,000 individuals were trained through this specific project operated by BIOTEC.


Thailand is considered a major producer and exporter of tapioca and starch. Although the native starch production business became very competitive in the recent past, most of the native starch factories need to make improvements on effective production processes, pollution control mechanisms, waste recycling/re-use, and energy efficiency options.

CAPACITY BUILDING ON ENERGY AND RESOURCE EFFICIENCY FOR THAI NATIVE STARCH INDUSTRY

Equipped with in-depth knowledge in biogas technology and tapioca starch industrial production processes and waste management, the Excellent Center of Waste Utilization and Management (ECoWaste), a joint unit between BIOTEC and King Mongkut’s University of Technology Thonburi, launched the project “Capacity Building on Energy and Resource Efficiency for Thai


Native Starch Industry” in May 2009 with financial support from NSTDA and GTZ. The 2-year project aims to improve the competitiveness of Thai native tapioca starch industry through a practical training program targeting plant owners, managers and unit operators. To enhance the learning process, two guidelines for eco-efficiency improvement will be developed, namely (i) best practice guide for eco-efficiency improvement in native starch industry, and (ii) management information system (MIS) guidelines for eco-efficiency for native starch industry.

In 2010, the Project launched a website Thailand Tapioca Starch (http://www.thailandtapiocastarch.net)

to serve as a source of information and knowledge for researchers and practitioners in the tapioca industry. The website is available in both Thai and English languages. In addition to the website, newsletters have been regularly produced and distributed amongst members of the network, which currently includes more than 50 individuals, 25 factories, and 20 engineering graduates. During June – August 2010, three workshops were conducted targeting three major stakeholders, namely factory managers and operators; public and private entities (including environmental consulting firms); and graduate students who plan to develop a career in this field.


Science in Rural Schools Program (SiRS) The Program is dedicated to youth in the rural areas covering the following provinces; Maehongson, Nan, Chiang Mai, Phrae, Chiang Rai, Sakon Nakhon, Pang-Nga and Narathiwat. SiRS aims to improve the quality of life and enhance learning capability in science for rural

YOUTH PROGRAM

students through activities such as science camps, workshops and science project contests. The following activities were organized in FY 2010:

• A workshop for school administrators and teachers in Chiang Mai to train them in techniques for teaching science was held in


November 2009. • A science camp with a banana theme was

held for students and villagers in Sakon Nakhon in May 2010.

• A training workshop to train science teachers in Nan to create teaching-aid media for science curriculum was held twice in August 2010.

• A project to develop student leaders was organized. Two groups of leaders were chosen, one on leaders in science and the other on leaders in environmental conservation.

Projects Supported by the Thai Health Promotion Foundation BIOTEC manages two projects funded by the Thai Health Promotion Foundation, namely:

• Science for Good Health The project aims to supplement teachers in the target area with heath-related scientific knowledge and engage students in science activities. Phase I of the Project, implemented between July 2007- March 2009, covered 224 schools and 666 teachers. As a result of the project, 130 science camps were organized and 561 science projects were initiated. With the success of Phase I, the Thai Health Promotion Foundation extended the project into Phase II until 2011. Under Phase II, seven workshops were conducted, with participation by science teachers from over 100 schools. The activities generated 269 science projects, and a science camp was held in Chiang Rai Province, with participation of 14 schools entering the science competition.

• Development of Local Science Curriculum for Southern Border Provinces A set of eleven children’s books were developed for students in this region under the theme “Living a Happy

and Healthy Life”. The content was developed by BIOTEC together with local school teachers and students dealing with health issues, incorporating the wisdom and knowledge in living a healthy life. The books were subsequently reviewed and endorsed by academic experts. 5,000 copies of the books were printed and introduced in the second semester of 2010 academic year.



PUBLIC AWARENESS

Striving to make Thailand a knowledge-based economy, one of BIOTEC’s missions is to create public awareness in biotechnology, especially relating biotechnology and life sciences to everyday living. The mission is carried out by channeling information through various available media such as internet and television, as well as children’s books.


Wonderful World A children’s book on genetically-modified microorganisms was released in 2010. This is the fourth book in the series “Wonderful World” to educate children on basic biology and biotechnology, with simple content, illustrations and daily-life examples. Three previous books were on microorganisms, DNA and biodiversity.

Living a Happy and Healthy Life Living a Happy and Healthy Life is a set of eleven children books prepared under the project “Development of Local

BOOKS

Science Curriculum for Southern Border Provinces” funded by the Thai Health Promotion Foundation. The content was developed by BIOTEC together with local school teachers and students dealing with health issues and incorporating the wisdom and knowledge in living a healthy life. 5,000 copies of the books were printed and introduced to schools in the four southern border provinces during the 2010 academic year.


A new version of the Thai-language corporate website was launched in January 2010. In addition to providing information on the organization itself, the BIOTEC website is filled with knowledge and information on biotechnology and its application, catering to a wide-spectrum of audiences from children to adults, from layman to technical persons to entrepreneurs. For educational purposes, an update on world-wide biotechnology in the form of articles is regularly posted on the website.

WEBSITE TELEVISION PROGRAM

NSTDA produces documentaries for TV broadcasting, some of which cover BIOTEC activities:

Short Documentary The 3-minute documentary is shown as part of the Science TV Program, aired on Channel 9. Documentaries introducing biotechnology topics include: BIOTEC researcher - Winner of L’Oreal For Women in Science, microtube gel test kit and BIOTEC researcher - Winner of Young Scientist Award.

Chowwit Chit Chowbaan TV Program Chowwit Chit Chowbaan is a 1-hr documentary aiming to introduce research that can have an impact on the public, especially farmers. The show is aired every Friday from 11:05-11:55 on the Thai PBS Channel and on public holidays from 11:00-11:30 on Channel 9. Seventeen episodes on biotechnology topics were produced and aired in FY 2010, on the following topics: rice seed production technology, sugarcane variety improvement through biotechnology, germinated brown rice, fish sausage, biogas technology, screening for disease-resistant cucumber, surimi: fish-based food product and feed additive for growing marine animals.



INTERNATIONAL COLLABORATION

The BIOTEC International Cooperation Program aims to capitalize on international linkages to make BIOTEC and Thailand a regional leader in the field of biotechnology. In so doing, the Center has developed close linkages with overseas organizations at the bilateral, multilateral and regional levels. The goal is achieved through activities such as establishing formal collaborative agreements, organizing joint scientific seminars with international partners, establishing and organizing an annual meeting of the BIOTEC International Advisory Board and showcasing BIOTEC and Thailand’s biotechnology in international events.


BIOTEC entered into MOUs with 3 institutes to promote research collaboration and staff / student exchange: • Temasek Polytechnic, Singapore – BIOTEC started

hosting students from Temasek Polytechnic in BIOTEC laboratories in 2007. The program grew steadily over the years, leading to formalization of the collaboration through an MOU in 2010.

• Queen’s University of Belfast, Northern Ireland

FOSTERING RESEARCH AND ACADEMIC COLLABORATION

– The first joint research project started in 2010 under this MOU framework involves the development of multiplex detection for plant diseases in melon using antibody array technology in a multiwell-plate format and multiplex SPR.

• University of North Texas Health Science Center at Fort Worth, USA – Under this MOU framework, BIOTEC has licensed the use of iCollect, software


developed by the BIOTEC Information Systems Laboratory to support the laboratory’s inventory management of biological collections for academic purposes at UNT.

To continue collaboration with existing partners, BIOTEC renewed MOUs with the following organizations in 2010:• Korea Research Institute for Bioscience and

Biotechnology (KRIBB) -- The collaboration with KRIBB started in 2004, with a regular joint seminar to enhance research collaboration. Enzyme technology is one of the active research topics between the two parties.

• Atma Jaya Catholic University, Indonesia - The first MOU with Atma Jaya Catholic University was formalized in 2005. Since then, BIOTEC labs regularly host students for research internship.

• Institute of Biotechnology (IBT), Vietnam - The MOU signed in 2005 led to a joint research project on “A/H5N1 influenza virus in Vietnam and Thailand: Molecular Epidemiology, Diagnosis and Vaccines” funded by BIOTEC and the Vietnamese Ministry of Science and Technology since March 2008. A new project on “Large-scale sequencing and bioinformatics analysis of express sequence tags (ESTs) and partial genomic sequences of the giant tiger shrimp (Penaeus monodon) using 454 pyrosequencing technology” has been initiated through two workshops held in March and September 2010 in Thailand and Vietnam, respectively. The new project is scheduled to commence in 2011, with funding from BIOTEC and the Vietnamese Ministry of Science and Technology.

Two joint seminars were organized:• The 5th BIOTEC and KRIBB Joint Symposium

on Bioresources Utilization and Management on 16 October 2009, organized in collaboration with the Korea Research Institute for Bioscience and Biotechnology (KRIBB)

• Symposium on “Malaria R&D Financing, Policy Innovation and Emerging Drug Resistance in Southeast Asia - Perspectives from the Local and Global” on 9 - 10 November 2009, organized in collaboration with the University of Cambridge, the UK Economic and Social Research Council (ESRC), United Nations Educational and Scientific and

Cultural Organization (UNESCO) Bangkok Office.• UK-Brazil-Thailand Joint Seminar on Agricultural

Biotechnology” on 22 - 24 February 2010, organized in collaboration with the UK Biotechnology and Biological Sciences Research Council (BBSRC) and the British High Commission in Singapore.


STRENGTHENING RICE BREEDING PROGRAM IN MEKONG REGION

The program obtained research funding from the Generation Challenge Program (GCP) in 2007-2008 under the project title “Community of Practices - Concept Applied to Rice Production in the Mekong Region: Quick conversion of popular rice varieties with emphasis on drought, salinity and grain quality improvement”

In November 2009, the continuation of this project titled “Community of Practices – Strengthening rice breeding program using genotyping building strategy and improving phenotyping capacity for biotic and abiotic stresses in the Mekong region” was approved for funding from GCP.

Partners in this Program include the Cambodian Agricultural Research and Development Institute, (CARDI), National Agricultural and Forestry Institute (NAFRI) from Laos, the Department of Agricultural Research (DAR) from Myanmar and the Ubon Ratchathani University from Thailand.

The Molecular Rice Breeding Program for the Mekong Region was launched in 2004 by the Rice Gene Discovery Unit aiming to promote the implementation of marker aided selection (MAS) into the current rice breeding programs in the Mekong region, particularly Thailand, Laos, Cambodia and Myanmar through a comprehensive hands-on training program and sharing of genomic information (genetic data for several traits and the molecular markers for several genes) and research facilities. The program was designed to address the limitation of short-term workshops which cannot leave participants with full understanding of the technology and laboratory skills, as well as the limitation of research facilities in these countries. To ensure the benefit of this program to each Mekong country, participants perform the MAS breeding to improve their own rice varieties.


REGIONAL R&D AND TRAINING HUB

With over two decades of operation, BIOTEC has built confidence in its high-caliber researchers and R&D infrastructure and attempts to utilize this expertise and facilities to build up research and technical capacity for the world community. The Center provides foreign students and researchers in the field of biotechnology and life sciences with opportunities to have hands-on experience conducting research in a professional environment. • Human Resource Development Program for

Neighboring Countries Initiated in 2001, the Program provides fellowships to young scientists from developing countries in the Asia-Pacific region to work in BIOTEC laboratories for 3-6 months. In FY 2010, out of 89 applicants from 10 countries, 12 fellowships were provided to researchers from Myanmar, Lao PDR, Vietnam, the Philippines, Mongolia and Indonesia. The survey conducted on past alumni, reaching 100 alumni this year, has demonstrated the success of the program. At least twenty eight alumni

obtained scholarships to further their education outside their home countries such as Australia, Austria, Japan, Korea, the Netherlands, the Philippines and USA. Most of them are making progress in their research work and continue focusing on their research career paths.

• International Exchange Program This Program is designed for hosting foreign students to have hands-on experience conducting research in a professional environment, as well as for foreign researchers who would like to obtain some training in particular areas. Fifty foreign students and researchers, both new and on-going, were hosted in FY2010 from institutes such as Atma Jaya Catholic University, Indonesia; University of Kent, UK; Temasek Polytechnic, Singapore; Nanyang Polytechnic, Singapore; University of Monpellier, France. This year marked the first participation by the University of Liverpool, sending 5 students to join the Program.


BIOTEC, in collaboration with the Thai public and private sectors, organized the Thailand Pavilion at the 2010 Bio International Convention held in Chicago on 3 – 6 May 2010.

This year, the Thailand Pavilion focused on Green & Clean Technology to showcase the nation’s abundant potential in utilizing its natural resources and home-grown strong R&D base to develop green technology to support the nation’s growing economy.

Highlights of the Thailand Pavilion also included the launch of a publication on Life Sciences in Thailand, as well as a mini–forum with talks covering various topics such as “Biotechnology R&D in Thailand” and “Biotech Business Opportunities in Asia”, with speakers

THAILAND PAVILION AT BIO INTERNATIONAL CONVENTION 2010

from the Board of Investment, Thailand Science Park and some foreign companies in Thailand.

Thailand was among fifteen countries selected to make a presentation in the International Case Studies track during the Convention. The presentation showed working mechanisms to synergize the efforts and identify opportunities in R&D in Thailand, using an R&D joint venture between Thailand’s BIOTEC and NanoDetection Technology, an American company, as an example.

The Thai delegation consisted of 13 organizations, including government authorities in R&D and investment, academic institutes as well as local and multinational companies.


LIFE SCIENCES IN THAILAND

BIOTEC spearheaded the publication of Life Sciences in Thailand, the first ever supplement with a major international magazine devoted to biotechnology and life sciences in Thailand, providing a comprehensive look into life sciences policy, industry and research in Thailand. 80,000 copies were printed for global distribution. The supplement was unveiled at Bio International Convention 2010 held during 3-6 May 2010 in Chicago, USA, and released with the May issue of the Scientist. Joining force with BIOTEC to finance this publication were various scientific organizations, universities, government agencies and the private sector, both local and multinational. The publication is available for download from the websites of BIOTEC, the Scientist and sponsors.

Life Sciences in Thailand



IMPACT OF BIOTEC’S OUTPUT

BIOTEC constantly monitors and assesses the impact of its output towards social and economic development. Every year, BIOTEC selects a number of technology transfer projects for detailed impact study. The impact is measured in the form of income generated by the clients out of such products and technologies, and also quantified from parameters such as import substitution and employment generation.


Micropropagation of Disease-free Sugarcane During 2003-2005, in response to a request from Mitr Phol Research and Development Center Co., Ltd., a research and development arm of the Mitr Phol Sugar Group, BIOTEC provided technical assistance to the company in establishing a tissue culture laboratory for sugarcane. Following this initial collaboration, BIOTEC provided continuous assistance to the company to help

EXAMPLES OF IMPACT FROM PROJECTS IN AGRICULTURE AND FOOD

build up their research capability through training and research collaboration and to help the company expand its sugarcane production. During the production year 2009/2010, the company was able to earn an additional 32 million Baht as a result of the production expansion. The expansion also created an additional 3.7 million Baht in employment value.


Germplasm Evaluation of Sugarcane Elite Lines across the Country In 2006, BIOTEC-NSTDA launched a project to evaluate sugarcane elite lines developed by 5 major sugarcane research centers through field trials in various areas by working in partnership with research stations across the country. The result of field trials assured the quality of the lines among farmers, resulting in a plantation expansion of new lines in 300,000 rai (48,000 hectares) equivalent to 3 billion Baht in income earned from the additional yield these elite lines can provide.

Virus Resistant Hybrid Okra, UNIH109 In 2004, BIOTEC collaborated with Uniseeds Co., Ltd. to develop virus resistant okra. Through the collaboration, the company was able to develop a new variety, UNIH109, yielding 2,500 – 3,000 kg/rai (15.63 – 18.75 tonnes/hectare), while the average yield was 1,000 kg/rai (6.25 tonnes/hectare). In 2010, the company earned 3.5 million Baht in sales value for UNIH109, and the value of okra production, estimated from the seed volume sold, would reach 138.3 billion Baht for the farmers.

Air Filter for Tissue Culture Laboratory BIOTEC researchers have developed an air filter for greenhouses or tissue culture containers, creating an environmental condition to allow for air and moisture exchange in the tissue culture process. The technology was licensed to Nisapan Co., Ltd., a tissue culture business, in March 2008 for 5 years. The company made 0.3 million Baht selling this product in 2010. The air filter helps reduce plantlet loss during the acclimatization period by 30%, the surviving plantlets being equivalent to 3 million Baht. As the equivalent imported product costs 5-6 times more,

this locally-made air filter was able to save the country 1.96 million Baht in foreign exchange costs.

Rapid Test Kit for Sugarcane White Leaf Disease BIOTEC, NSTDA and Mitr Phol Research and Development Center Co., Ltd., have jointly developed a rapid test kit for phytoplasma infection, which is the cause of white leaf disease in sugarcane. The test kit allows farmers to screen for phytoplasma-free cane stalks before planting, which will not only reduce losses, but will also minimize the spread of disease to healthy plants. The test kit has been sold in Thailand, as well as neighboring countries such as Laos and Cambodia, with a sales value of 1.15 million Baht. Steroid-based Synchronization of Ovulation Protocol and Fixed Time Artificial Insemination BIOTEC researchers have transferred the technology to farmers for ovulation synchronization, using hormone and artificial insemination, through training workshops and follow-up consultancy. The training and technical assistance has enabled the farmers to induce the pregnancy in 3,164 cows which previously have reproductive system problems, adding 20,000 Baht to the value of each cow. As cows reach lactation, additional income from milk production can also be earned. The total monetary impact is estimated to be over 270 million Baht.


EXAMPLES OF IMPACT FROM PROJECTS IN HEALTH AND MEDICINE

CD4+ Lymphocytes Enumeration Assay The assay for enumeration of CD4+ lymphocytes was developed by the Biomedical Technology Research Center, a joint laboratory between BIOTEC and Chiang Mai University. The assay does not require an expensive flow cytometer, but instead uses a hematoanalyzer, which is common equipment in general hospitals. The technology was licensed to i+MED Laboratories Co., Ltd. In 2010, i+MED Laboratories earned 0.24 million Baht in revenue. This locally-made product was able to save the country 0.53 million Baht from imports and the cost of eliminating the purchase of flow cytometers is estimated at 0.26 million Baht.

Test for Red Cell Antigen-Antibody Detection BIOTEC-NSTDA provided a research grant to Khon Kaen University to develop a test for red cell antigen-antibody detection. The test can be mass produced domestically at a relatively low cost, allowing for a cost of 5-10 Baht/test, while the imported test can cost up to 25 Baht. The technology was licensed to Innov (Thailand) Co., Ltd. and the product was released in the market in January 2010, contributing 0.94 million Baht in sales to the company. The saving from import costs for this product is estimated at 3.7 million Baht.


EXAMPLES OF IMPACT FROM PROJECTS IN BIORESOURCES UTILIZATION AND ENVIRONMENT

Pentosanase Production Technology BIOTEC has developed the production technology which uses the pentosanase-producing microorganism carefully screened from the BIOTEC Culture Collection and the process to achieve a final product in powder form. The technology was licensed to Asia Star Animal Health Co., Ltd. (ASAH) to manufacture and distribute pentosanase as a feed additive. The company earned 2.4 million Baht in sales from the product, saving the country 4.8 million Baht/year from importing this enzyme. The impact from healthier animals, better meat quality and the saving on feed is estimated to be 22.35 million Baht.

Promotion of Biogas Technology in Cassava Starch Industry Biogas technology for wastewater treatment and methane production was developed by EcoWaste and implemented in 3 factories, resulting in 43.62 million Baht in energy savings and unaccounted environmental benefits.

Promotion of Biogas Technology in Agri-food Industry The fixed-film type of biogas technology, designed by EcoWaste, was installed in 2 factories making dry fruit products. The value of waste treatment cost saving, energy saving and electricity generation totaled 30 million Baht.

Biogas Technology in Palm Oil Industry The research and engineering team at EcoWaste has designed and installed a combination of fixed-bed and sludge-bed reactor, suitable for wastewater from palm oil production in Thachana Palm Oil Co., Ltd, adding to an existing reactor. The new reactor helps increase the stability of the wastewater systems of the factory, as well as accommodating a higher loading rate. Biogas produced is valued at 1.2 million Baht/year.

Development of Zero Waste Discharge Cassava Starch Factory Researchers and engineers from EcoWaste have been continuously developing technologies to improve production processes, pollution control mechanisms, waste recycling/re-use and energy efficiency options. In 2010, researchers were able to make an improvement on an extractor and recover more starch from water discharged from a separator at Cholcharoen Factory, resulting in an additional 33.34 million Baht from the resultant yield increase and savings.

APPENDICES

List of PublicationsList of Patents and Petty Patents Honors and AwardsExecutives and Management Team


1. Abdel-Wahab, M.A., Pang, K.-L., Nagahama, T., Abdel-Aziz, F.A. and Jones, E.B.G. (2010). Phylogenetic evaluation of anamorphic species of Cirrenalia and Cumulospora with the description of eight new genera and four new species. Mycological Progress, doi: 10.1007/s11557-010-0661-x.

2. Abdulla, M.A., Ahmed, I., Assawamakin, A., Bhak, J., Brahmachari, S.K., Calacal, G.C., Chaurasia, A., Chen, C.H., Chen, J., Chen, Y.T., Chu, J., Cutiongco-de la Paz, E.M.C., De Ungria, M.C.A., Delfin, F.C., Edo, J., Fuchareon, S., Ghang, H., Gojobori, T., Han, J., Ho, S.F., Hoh, B.P., Huang, W., Inoko, H., Jha, P., Jinam, T.A., Jin, L., Jung, J., Kangwanpong, D., Kampuansai, J., Kennedy, G.C., Khurana, P., Kim, H.L., Kim, K., Kim, S., Kim, W.Y., Kimm, K., Kimura, R., Koike, T., Kulawonganunchai, S., Kumar, V., Lai, P.S., Lee, J.Y., Lee, S., Liu, E.T., Majumder, P.P., Mandapati, K.K., Marzuki, S., Mitchell, W., Mukerji, M., Naritomi, K., Ngamphiw, C., Niikawa, N., Nishida, N., Oh, B., Oh, S., Ohashi, J., Oka, A., Ong, R., Padilla, C.D., Palittapongarnpim, P., Perdigon, H.B., Phipps, M.E., Png, E., Sakaki, Y., Salvador, J.M., Sandraling, Y., Scaria, V., Seielstad, M., Sidek, M.R., Sinha, A., Srikummool, M., Sudoyo, H., Sugano, P., Suryadi, H., Suzuki, Y., Tabbada, K.A., Tan, A., Tokunaga, K., Tongsima, S., Villamor, L.P., Wang, E., Wang, Y., Wang, H., Wu, J.Y., Xiao, H., Xu, S., Yang, J.O., Shugart, Y.Y., Yoo, H.S., Yuan, W., Zhao, G., Zilfalil, B.A. and Indian Genome Variation Consortium. (2009). Mapping Human Genetic Diversity in Asia. Science, 326(5959), 1541-1545.

3. Aewsiri, T., Benjakul, S., Visessanguan, W., Wierenga, P.A. and Gruppen, H. (2010). Antioxidative activity and emulsifying properties of cuttlefish skin gelatin-tannic acid complex as influenced by types of interaction. Innovative Food Science and Emerging Technologies, doi:10.1016/j.ifset.2010.04.001.

4. Am-In, S., Limtong, S., Yongmanitchai, W. and Jindamorakot, S. (2010). Candida andamanensis sp. nov., Candida laemsonensis sp. nov., and Candida ranongensis sp. nov., three anamorphic yeast species isolated from estuarine waters in a mangrove forest in Ranong Province, Thailand. International Journal of Systematic and Evolutionary Microbiology, doi:10.1099/ijs.0.022038-0.

5. Amparyup, P., Wiriyaukaradecha, K., Charoensapsri, W. and Tassanakajon, A. (2010). A clip domain serine proteinase plays a role in antibacterial defense but is not required for prophenoloxidase activation in shrimp. Developmental and Comparative Immunology, 34(2), 168-176.

6. Angthong, P., Watthanasurorot, A., Klinbunga, S., Ruangdej, U., Söderhäll, I. and Jiravanichpaisal, P. (2010). Cloning and characterization of a melanization inhibition protein (PmMIP) of the black tiger shrimp, Penaeus monodon. Fish and Shellfish Immunology, 29(3), 464-468.

7. Anuchapreeda, S., Tima, S., Duangrat, C. and Limtrakul, P. (2008). Effect of pure curcumin, demethoxycurcumin,

LIST OF PUBLICATIONS

and bisdemethoxycurcumin on WT1 gene expression in leukemic cell lines. Cancer Chemotherapy and Pharmacology, 62(4), 585-594.

8. Arunpanichlert, J., Rukachaisirikul, V., Sukpondma, Y., Phongpaichit, S., Tewtrakul, S.,Rungjindamai, N. and Sakayaroj, J. (2010). Azaphilone and isocoumarin derivatives from the endophytic fungus Penicillium sclerotiorum PSU-A13. Chemical and Pharmaceutical Bulletin, 58(8), 1033-1036.

9. Banyai, W., Kirdmanee, C., Mii, M. and Supaibulwatana, K. (2010). Overexpression of farnesyl pyrophosphate synthase (FPS) gene affected artemisinin content and growth of Artemisia annua L. Plant Cell Tissue and Organ Culture, doi:10.1007/s11240-010-9775-8.

10. Benjakul, S., Thiansilakul, Y., Visessanguan, W., Roytrakul, S., Kishimura, H., Prodpran, T. and Meesane, J. (2010). Extraction and characterisation of pepsin-solubilised collagens from the skin of bigeye snapper (Priacanthus tayenus and Priacanthus macracanthus). Journal of the Science of Food and Agriculture, 90(1), 132-138.

11. Benjakul, S., Yarnpakdee, S., Visessanguan, W. and Phatcharat, S. (2010). Combination effects of whey protein concentrate and calcium chloride on the properties of goatfish surimi gel. Journal of Texture Studies, 41(3), 341-357.

12. Bertoft, E., Laohaphatanalert, K., Piyachomkwan, K. and Sriroth, K. (2010). The fine structure of cassava starch amylopectin. Part 2: Building block structure of clusters. International Journal of Biological Macromolecules, 47(3), 325-335.

13. Boondaeng, A., Suriyachadkun, C., Ishida, Y., Tamura, T., Tokuyama, S. and Kitpreechavanich, V. (2010). Herbidospora sakaeratensis sp. nov., isolated from Thailand and re-classification of Streptosporangium claviforme as a later synonym of Herbidospora cretacea. International Journal of Systematic and Evolutionary Microbiology, doi:10.1099/ijs.0.024315-0.

14. Boonmak, C., Limtong, S., Jindamorakot, S., Am-In, S., Yongmanitchai, W., Suzuki, K.I., Nakase, T. and Kawasaki, H. (2010). Candida xylanilytica sp. nov., a xylan degrading yeast species isolated from Thailand. International Journal of Systematic and Evolutionary Microbiology, doi:10.1099/ijs.0.021873-0.

15. Boonyos, P., Soonsanga, S., Boonserm, P. and Promdonkoy, B. (2010). Role of cysteine at positions 67, 161 and 241 of a Bacillus sphaericus binary toxin BinB. Journal of Biochemistry and Molecular Biology, 43(1), 23-28.

16. Buaban, B., Inoue, H., Yano, S., Tanapongpipat, S., Ruanglek, V., Champreda, V., Pichyangkura, R., Rengpipat, S. and Eurwilaichitr, L. (2010). Bioethanol production from ball milled bagasse using an on-site produced fungal enzyme cocktail and xylose-fermenting Pichia stipitis. Journal of Bioscience and Bioengineering, doi: 10.1016/j.jbiosc.2009.12.003.

17. Bunterngsook, B., Kanokratana, P., Thongaram, T., Tanapongpipat, S., Uengwetwanit, T., Rachdawong, S.,


Vichitsoonthonkul, T. and Eurwilaichitr, L. (2010). Identification and Characterization of Lipolytic Enzymes from a Peat-Swamp Forest Soil Metagenome. Bioscience Biotechnology and Biochemistry, 74(9), 1848-1854.

18. Bunyapaiboonsri, T., Yoiprommarat, S., Srikitikulchai, P., Srichomthong, K. and Lumyong, S. (2010). Oblongolides from the Endophytic Fungus Phomopsis sp. BCC 9789. Journal of Natural Products, 73(1), 55-59.

19. Cerenius, L., Babu, R., Söderhäll, K. and Jiravanichpaisal, P. (2010). In vitro effects on bacterial growth of phenoloxidase reaction products. Journal of Invertebrate Pathology, 103(1), 21-23.

20. Chaiklahan, R., Chirasuwan, N., Siangdung, W., Paithoonrangsarid, K. and Bunnag, B. (2010). Cultivation of Spirulina platensis using pig wastewater in a semi-continuous process. Journal of Microbiology and Biotechnology, 20(3), 609-614.

21. Chaiprasert, A., Krajaejun, T., Pannanusorn, S., Prariyachatigul, C., Wanachiwanawin, W., Sath patayavongs, B., Juthayothin, T., Smittipat, N., Vanittanakom, N. and Chindamporn, A. (2010). Pythium insidiosum Thai isolates: molecular phylogenetic analysis. Asian Biomedicine, 3(6), 623-633.

22. Chaivisuthangkura, P., Longyant, S., Hajimasalaeh, W., Sridulyakul, P., Rukpratanporn, S. and Sithigorngul, P. (2010). Improved sensitivity of Taura syndrome virus immunodetection with a monoclonal antibody against the recombinant VP2 capsid protein. Journal of Virological Methods, 163(2), 433-439.

23. Chaivisuthangkura, P., Longyant, S., Rukpratanporn, S., Srisuk, C., Sridulyakul, P. and Sithigorngul, P. (2010). Enhanced white spot syndrome virus (WSSV) detection sensitivity using monoclonal antibody specific to heterologously expressed VP19 envelope protein. Aquaculture, 299(1-4), 15-20.

24. Chaivisuthangkura, P., Srisuk, C., Rukpratanporn, S., Longyant, S., Sridulyakul, P. and Sithigorngul, P. (2010). Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus by loop-mediated isothermal amplification. Journal of Virological Methods, 162(1-2), 188-193.

25. Chamroensaksri , N., Tanasupawat, S., Akaracharanya, A., Visessanguan, W., Kudo, T. and Itoh, T. (2009). Salinivibrio siamensis sp. nov., from fermented fish (pla-ra) in Thailand. International Journal of Systematic and Evolutionary Microbiology, 59(4), 880-885.

26. Chamroensaksri, N., Tanasupawat, S., Akaracharanya, A., Visessanguan, W., Kudo, T. and Itoh, T. (2010). Gracilibacillus thailandensis sp. nov., from fermented fish (pla-ra). International Journal of Systematic and Evolutionary Microbiology, 60(4), 944-948.

27. Chanama, M., Chanama, S., Shaw, P.J., Chitnumsub, P., Leartsakulpanich, U. and Yuthavong, Y. (2010). Formation of catalytically active cross-species heterodimers of thymidylate synthase from Plasmodium falciparum and Plasmodium vivax. Molecular Biology Reports, doi:10.1007/s11033-010-0199-7.

28. Chareonlimkun, A., Champreda, V., Shotipruk, A.

and Laosiripojana, N. (2010). Catalytic conversion of sugarcane bagasse, rice husk and corncob in the presence of TiO

2, ZrO

2 and mixed-oxide TiO

2-ZrO

2 under

hot compressed water (HCW) condition. Bioresource Technology, 101(11), 4179-4186.

29. Chareonlimkun, A., Champreda, V., Shotipruk, A. and Laosiripojana, N. (2010). Reactions of C

5 and C

6-sugars,

cellulose, and lignocellulose under hot compressed water (HCW) in the presence of heterogeneous acid catalysts. Fuel, 89(10), 2873-2880.

30. Charerntantanakul, W. and Kasinrerk, W. (2010). Interleukin-10 antisense oligodeoxynucleotide suppresses IL-10 expression and effects on proinflammatory cytokine responses to porcine reproductive and respiratory syndrome virus. Viral Immunology, 23(4), 425-435.

31. Charlermroj, R., Gajanandana, O., Barnett, C., Kirtikara, K. and Karoonuthaisiri, N. (2010). A chemiluminescent antibody array system for detection of foodborne pathogens in milk. Analytical Letters, doi:10.1080/00032719.2010.511736.

32. Charoensapsri, W., Amparyup, P., Hirono, I., Aoki, T. and Tassanakajon, A. (2010). PmPPAE2, a new class of crustacean prophenoloxidase (proPO)-activating enzyme and its role in PO activation. Developmental and Comparative Immunology, doi:10.1016/j.dci.2010.09.002.

33. Charoenthaikij, P., Jangchud, K., Jangchud, A., Piyachomkwan, K., Tungtrakul, P. and Prinyawiwatkul, W. (2009). Germination conditions affect physicochemical properties of germinated brown rice flour. Journal of Food Science, 74(9), c658-c665.

34. Cha-um S. and Kirdmanee, C. (2010). Salt tolerance screening in six maize (Zea mays L.) genotypes using multivariate cluster analysis. Philippine Agricultural Scientist, 93(2), 156-164.

35. Cha-um S., Ashraf, M. and Kirdmanee, C. (2010). Screening upland rice (Oryza sativa L. ssp. indica) genotypes for salt-tolerance using multivariate cluster analysis. African Journal of Biotechnology, 9(30), 4731-4740.

36. Cha-um, S. and Kirdmanee, C. (2009). Proline Accumulation, Photosynthetic Abilities and Growth Characters of Sugarcane (Saccharum officinarum L.)Plantlets in Response to Iso-Osmotic Salt and Water-Deficit Stress. Agricultural Science in China, 8(1), 51-58.

37. Cha-um, S. and Kirdmanee, C. (2010). Effect of glycinebetaine on proline, water use and photosynthetic efficiencies and growth of rice seedlings under salt stress. Turkish Journal of Agriculture and Forestry, doi:10.3906/ tar-0906-34.

38. Cha-um, S. and Kirdmanee, C. (2010). Effects of water stress induced by sodium chloride and mannitol on proline accumulation, photosynthetic abilities and growth characters of eucalyptus (Eucalyptus camaldulensis Dehnh.). New Forests, doi:10.1007/s11056-010-9204-1.

39. Cha-um, S., Boriboonkaset, T., Pichakum, A. and Kirdmanee, C. (2009). Multivariate physiological indices for salt tolerant classification in indica rice (Oryza sativa L. spp.


indica). General and Applied Plant Physiology, 35(1-2), 75-87.

40. Cha-Um, S., Nhung, N.T.H. and Chalermpol, K. (2010). Effect of mannitol- and salt-induced iso-osmotic stress on proline accumulation, photosynthetic abilities and growth characters of rice cultivars (Oryza sativa L. spp. Indica). Pakistan Journal of Botany, 42(2), 927-941.

41. Cha-Um, S., Siringam, K., Juntawong, N. and Kirdmanee, C. (2010). Water relations, pigment stabilization, photosynthetic abilities and growth improvement in salt stressed rice plants treated with exogenous potassium nitrate application. International Journal of Plant Production, 4(3), 187-198.

42. Cha-um, S., Supaibulwatana, K. and Kirdmanee, C. (2009). Comparative Effects of Salt Stress and Extreme pH Stress Combined on Glycinebetaine Accumulation, Photosynthetic Abilities and Growth Characters of Two Rice Genotypes.Rice Science, 16(4), 274-282.

43. Cha-Um, S., Takabe, T. and Kirdmanee, C. (2010). Ion contents, relative electrolyte leakage, proline accumulation, photosynthetic abilities and growth characters of oil palm seedlings in response to salt stress. Pakistan Journal of Botany, 42(3), 2191-2020.

44. Cha-um, S., Thadavong, S. and Kirdmanee C. (2009). Effects of mannitol induced osmotic stress on proline accumulation, pigment degradation, photosynthetic abilities and growth characters in C3 rice and C4 sorghum. Frontiers of Agriculture in China, 3(3), 266-273.

45. Cha-um, S., Trakulyingcharoen, T., Smitamana, P. and Kirdmanee, C. (2009). Salt tolerance in two rice cultivars differing salt tolerant abilities in responses to iso-osmotic stress. Australian Journal of Crop Science, 3(4), 221-230.

46. Chiampanichayakul, S., Anuchapreeda, S., Chruewkamlow, N., Mahasongkram, K., Thanaratanakorn, P. and Kasinrerk, W. (2010). Production of monoclonal antibodies to P- glycoprotein: its application in detection of soluble and surface P-glycoprotein of leukemia patients. International Journal of Hematology, 92(2), 326-333.

47. Chiaraphongphon, S., Suriyachadkun, C., Tamura, T. and Thawai, C. (2010). Dactylosporangium maewongense sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 60(2010), 1200-1205.

48. Chitnumsub, P., Yuvaniyama, J., Chahomchuen, T., Vilaivan, T. and Yuthavong,Y. (2009). Crystallization and preliminary crystallographic studies of dihydrofolate reductase- thymidylate synthase from Trypanosoma cruzi, the Chagas disease pathogen. Acta Crystallographica Section F: Structural Biology and Crystallization Communications, 65(11),1175-1178.

49. Chokpaiboon, S., Sommit, D., Teerawatananond, T., Muangsin, N., Bunyapaiboonsri, T. and Pudhom, K. (2010). Cytotoxic Nor-chamigrane and Chamigrane Endoperoxides from a Basidiomycetous Fungus. Journal of Natural Products, 73(5), 1005-1007.

50. Chu, C., Woods, N., Sawasdee, N., Guizouarn, H., Pellissier, B., Borgese, F., Yenchitsomanus, P.T., Gowrishankar, M. and Cordat, E. (2010). Band 3 Edmonton I, a novel

mutant of the anion exchanger 1 causing spherocytosis and distal renal tubular acidosis. Biochemical Journal, 426, 379-388.

51. Chusattayanond, A.D.-a., Boonsilp, S., Kasisit, J., Boonmee, A. and Warit, S. (2010). Thai Acanthamoeba isolate (T4) induced apoptotic death in neuroblastoma cells via the Bax-mediated pathway. Parasitology International, doi:10.1016/j.parint.2010.06.007.

52. Chutipaijit, S., Cha-um, S. and Sompornpailin, K. (2009). Differential accumulations of proline and flavonoids in indica rice varieties against salinity. Pakistan Journal of Botany, 41(5), 2497-2506.

53. Chutipaijit, S., Cha-um, S. and Sompornpailin, K. (2010). Proline accumulation and physiological responses of indica rice genotypes differ in tolerance to salt and drought stress. Philippine Agricultural Scientist, 93, 165-169.

54. Costin, J.M., Jenwitheesuk, E., Lok, S.M., Hunsperger, E., Conrads, K.A., Fontaine, K.A., Rees, C.R., Rossmann, M.G., Isern, S., Samudrala, R. and Michael, S.F. (2010). Structural Optimization and De Novo Design of Dengue Virus Entry Inhibitory Peptides. PLoS Neglected Tropical Diseases, doi:10.1371 journal.pntd.0000721.

55. Dejnirattisai, W., Jumnainsong, A., Onsirisakul, N., Fitton, P., Vasanawathana, S., Limpitikul, W., Puttikhunt, C., Edwards, C., Duangchinda, T., Supasa, S., Chawansuntati, K., Malasit, P., Mongkolsapaya, J. and Screaton, G. (2010). Cross-Reacting Antibodies Enhance Dengue Virus Infection in Humans. Science, 328(5979), 745-748.

56. Dikit, P., Methacanon, P., Visessanguan, W., H-kittikun, A. and Maneerat, S. (2010). Characterization of an unexpected bioemulsifier from spent yeast obtained from Thai traditional liquor distillation. International Journal of Biological Macromolecules, 47(4), 465-470.

57. Dupont, J., Magnin, S., Rousseau, F., Zbinden, M., Frebourg, G., Samadi, S., Richer de Forges, B. and Jones, E.B.G. (2009). Molecular and ultrastructural characterization of two ascomycetes found on sunken wood off Vanuatu Islands in the deep Pacific Ocean. Mycological Research, 113(12), 1351-1364.

58. Flegel, T.W. and Sritunyalucksana, K. (2010). Shrimp Molecular Responses to Viral Pathogens. Marine Biotechnology, doi:10.1007/s10126-010-9287-x.

59. Fugthong, A., Boonyapakron, K., Sornlek, W., Tanapongpipat, S., Eurwilaichitr, L. and Pootanakit, K. (2010). Biochemical characterization and in vitro digestibility assay of Eupenicillium parvum (BCC17694) phytase expressed in Pichia pastoris. Protein Expression and Purification, 70(1), 60-67.

60. Fusuwankaya, K., Chaiprasert, P. and Suraraksa, B. (2009). Treatment of modified starch wastewater with high sodium chloride (NaCl) concentration using an anaerobic hybrid reactor. Desalination and Water Treatment, 4, 224-228.

61. Gangnonngiw, W., Kanthong, N. and Flegel, T.W. (2010). Successful propagation of shrimp yellow head virus in immortal mosquito cells. Diseases of Aquatic Organisms, 90(1), 77-83.

62. Gangnonngiw, W., Laisutisan, K., Sriurairatana, S., Senapin,


S., Chuchird, N., Limsuwan, C., Chaivisuthangkura, P. and Flegel, T.W. (2010).Monodon baculovirus (MBV) infects the freshwater prawn Macrobrachium rosenbergii cultivated in Thailand.Virus Research, 148(1-2), 24-30.

63. Haritakun, R., Sappan, M., Suvannakad, R., Tasanathai, K. and Isaka, M. (2010). An Antimycobacterial Cyclodepsipeptide from the Entomopathogenic Fungus Ophiocordyceps communis BCC 16475. Journal of Natural Products, 73(1), 75-78.

64. Harnpicharnchai, P., Sornlake, W., Tang, K., Eurwilaichitr, L. and Tanapongpipat, S. (2010). Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement. FEMS Microbiology Letters. 302(1), 8-14.

65. Hongkachern, T., Champreda, V., Srikhirin, T., Wangkam, T. and Osotchan, T. (2010). Effect of pH on the Formation of a Bovine Serum Albumin Layer on a Poly (stryren-co- maleic Acid) Surface. Advanced Materials Research, 93-94(2010), 583-586.

66. Imanishi, Y., Jindamorakot, S., Limtong, S. and Nakase, T. (2010). Endospore formation in Hanseniaspora pseudoguilliermondii: A key characteristics of the species. Mycoscience, 51(5), 373-378.

67. Imjongjirak, C., Amparyup, P. and Tassanakajon, A. (2010). Molecular cloning, genomic organization and antibacterial activity of a second isoform of antilipopolysaccharide factor (ALF) from the mud crab, Scylla paramamosain. Fish and Shellfish Immunology, doi:10.1016/j.fsi.2010.09.011.

68. Intarapanich, A., Shaw, P.J., Assawamakin, A., Wan kumhang, P., Ngamphiw, C., Chaichoompu, K., Piriyapongsa, J. and Tongsima, S. (2009). Iterative pruning PCA improves resolution of highly structured populations. BMC Bioinformatics, 10, 382.

69. Isaka, M., Chinthanom, P., Boonruangprapa, T., Rungjindamai, N. and Pinruan, U. (2010). Eremophilane-Type Sesquiterpenes from the Fungus Xylaria sp. BCC 21097. Journal of Natural Products, 73(4), 683-687.

70. Isaka, M., Chinthanom, P., Kongthong, S., Supothina, S. and Ittiworapong, P. (2010). Hamigeromycins C–G, 14-membered macrolides from the fungus Hamigera avellanea BCC 17816. Tetrahedron, 66(4), 955-961.

71. Isaka, M., Sappan, M., Auncharoen, P. and Srikitikulchai, P. (2010). Chromone derivatives from the wood-decay fungus Rhizina sp. BCC 12292. Phytochemistry Letters, 3(3), 152-155.

72. Isaka, M., Yangchum, A., Rachtawee, P., Khoyaiklang, P., Boonyuen, N. and Lumyong, S. (2009). Dihydronaphthalenones from the endophytic fungus Botryosphaeria sp. BCC 8200. Phytochemistry Letters, 2(4), 207-210.

73. Isaka, M., Yangchum, A., Rachtawee, P., Komwijit, S. and Lutthisungneon, A. (2010). Hopane-Type Triterpenes and Binaphthopyrones from the Scale Insect Pathogenic Fungus Aschersonia paraphysata BCC 11964. Journal of Natural Products, 73(4), 688-692.

74. Jaichumjai, P., Valyasevi, R., Assavanig, A. and Kurdi, P. (2010). Isolation and characterization of acid-sensitive Lactobacillus plantarum with application as starter culture

for Nham production. Food Microbiology, 27(6), 741-748.75. Jariyapan, N., Baimai, V., Poovorawan, Y., Roytrakul, S.,

Saeung, A., Thongsahuan, S., Suwannamit, S., Otsuka, Y. and Choochote, W. (2010). Analysis of female salivary gland proteins of the Anopheles barbirostris complex (Diptera: Culicidae) in Thailand. Parasitology Research, doi:10.1007/s00436-010-1883-1.

76. Jeamton, W., Dulsawat, S., Laoteng, K., Tanticharoen, M. and Cheevadhanarak, S. (2010). Phycocyanin promoter of Spirulina platensis controlling heterologous expression in cyanobacteria. Journal of Applied Phycology, doi:10.1007/s10811-010-9540-8.

77. Jongkon, N., Mokmak, W., Chuakheaw, D., Shaw, P.J., Tongsima, S. and Sangma, C. (2009). Prediction of avian influenza A binding preference to human receptor using conformational analysis of receptor bound to hemagglutinin. BMC Genomics, 10(Suppl 3), S24.

78. Junjhon, J., Edwards, T.J., Utaipat, U., Bowman, V.D., Holdaway, H.A., Zhang, W., Keelapang, P., Puttikhunt, C., Perera, R., Chipman, P.R., Kasinrerk, W., Malasit, P., Kuhn, R.J. and Sittisombut, N. (2010). Influence of pr-M Cleavage on the Heterogeneity of Extracellular Dengue Virus Particles. Journal of Virology, 84(16), 8353-8358.

79. Kaewmanee, T., Benjakul, S. and Visessanguan, W. (2009). Protein Hydrolysate of Salted Duck Egg White as a Substitute of Phosphate and Its Effect on Quality of Pacific White Shrimp (Litopenaeus vannamei). Journal of Food Science, 74(8), s351-s361.

80. Kammarnjesadakul, P., Palaga, T., Sritunyalucksana, K., Mendoza, L., Krajaejun, T., Vanittanakom, N., Tongchusak, S., Denduangboripant, J. and Chindamporn, A. (2010). Phylogenetic analysis of Pythium insidiosum Thai strains using cytochrome oxidase II (COX II) DNA coding sequences and internal transcribed spacer regions (ITS). Medical Mycology, doi:10.3109/13693786.2010.511282.

81. Kanthong, N., Khemnu, N., Pattanakitsakul, S.-N., Malasit, P. and Flegel, T.W. (2010). Persistent, triple-virus co-infections in mosquito cells. BMC Microbiology, 10(art. no. 14).

82. Khongto, B., Laoteng, K. and Tongta, A. (2010). Fermentation process development of recombinant Hansenula polymorpha for gamma-linolenic acid production. Journal of Microbiology and Biotechnology, doi:10.4014/jmb.1003.03004.

83. Khouangvichit, S., Songkasiri, W., Stefan, C., Commins, T. and Touch, V. (2009). Survey on Participation of Nongtha Tai Villagers (Lao PDR) in Household Waste Management. Asian Journal of Energy and Environment, 10(2), 53-61.

84. Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. and Shahidi, F. (2009). Isolation and properties of acid- and pepsin-soluble collagen from the skin of blacktip shark (Carcharhinus limbatus). European Food Research and Technology, 230(3), 475-483.

85. Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. and Shahidi, F. (2010). Comparative study on characteristics of gelatin from the skins of brownbanded bamboo shark and blacktip shark as affected by


extraction conditions. Food Hydrocolloids, 24(2-3), 164-171.86. Kittiphattanabawon, P., Benjakul, S., Visessanguan, W.

and Shahidi, F. (2010). Effect of Extraction Temperature on Functional Properties and Antioxidative Activities of Gelatin from Shark Skin. Food and Bioprocess Technology, doi:10.1007/s11947-010-0427-0.

87. Kittiphattanabawon, P., Benjakul, S., Visessanguan, W. and Shahidi, F. (2010). Isolation and characterization of collagen from the cartilages of brownbanded bamboo shark (Chiloscyllium punctatum) and blacktip shark (Carcharhinus limbatus). Lwt-Food Science and Technol-ogy, 43(5), 792-800.

88. Kittiphattanabawon, P., Benjakul, S., Visessanguan, W., Kishimura, H. and Shahidi, F. (2010). Isolation and characterisation of collagen from the skin of brownbanded bamboo shark (Chiloscyllium punctatum). Food Chemistry, 119(4), 1519-1526.

89. Klinbunga, S., Sittikankaew, K., Yuvanatemiya, V., Preechaphol, R., Prasertlux, S., Yamano, K. and Menasveta, P. (2009). Molecular cloning and expression analysis of ovary-specific transcript 1 (Pm-OST1) of the giant tiger shrimp, Penaeus monodon. Zoological Science, 26(11), 783-790.

90. Klinbunga, S., Thamniemdee, N., Yuvanatemiya, V., Khetpu, K., Khamnamtong, B. and Menasveta, P. (2010). Species identification of the blue swimming crab Portunus pelagicus in Thai waters using mtDNA and RAPD-derived SCAR markers. Aquaculture, 308(Suppl.1), S39-S46.

91. Klinchid, J., Chewaskulyoung, B., Saeteng, S., Lertprasertsuke, N., Kasinrerk, W. and Cressey, R. (2009). Effect of combined genetic polymorphisms on lung cancer risk in northern Thai women. Cancer Genetics and Cytogenetics, 195(2), 143-149.

92. Kocharin, K., Rachathewee, P., Sanglier, J.-J. and Prathumpai, W. (2010). Exobiopolymer production of Ophiocordyceps dipterigena BCC 2073: Optimization, production in bioreactor and characterization. BMC Biotechnology, 10, 51.

93. Kommanee, J., Tanasupawat, S., Yukphan, P., Malimas, T., Muramatsu, Y., Nakagawa, Y. and Yamada, Y. (2010). Asaia spathodeae sp. nov., an acetic acid bacterium in the α-Proteobacteria. Journal of General and Applied Microbiology, 56(1), 81-87.

94. Kongprakhon, P., Cuesta-Marcos, A., Hayes, P.M., Hongtrakul, V., Sirithunya, P., Toojinda, T. and Sangduen, N. (2010). Four QTL in Rice associated with broad spectrum resistance to blast isolates from Rice and Barley. Journal of Phytopathology, 158(2), 125-131.

95. Kongprakhon, P., Cuesta-Marcos, A., Hayes, P.M., Richardson, K.L., Sirithunya, P., Sato, K., Steffenson, B. and Toojinda, T. (2009). Validation of rice blast resistance genes in barley using a QTL mapping population and near-isolines. Breeding Science, 59(4), 341-349.

96. Kornsakulkarn, J., Thongpanchang, C., Chainoy, R., Choowong, W., Nithithanasilp, S. and Thongpanchang, T. (2010). Bioactive Metabolites from Cultures of Basidiomycete Favolaschia tonkinensis. Journal of Natural Products, 73(4), 759-762.

97. Krajaejun, T., Keeratijarut, A., Sriwanichrak, K., Lowhnoo, T., Rujirawat, T., Petchthong, T., Yingyong, W., Kalambaheti, T., Smittipat, N., Juthayothin, T. and Sullivan, T.D. (2010). 74-kiloDalton Immunodominant Antigen of the Pathogenic Oomycete Pythium insidiosum is a Putative Exo-1,3-β Glucanase. Clinical and Vaccine Immunology, 17(8), 1203-1210.

98. Kunkeaw, S., Yoocha, T., Sraphet, S., Boonchanawiwat, A., Boonseng, O., Lightfoot, D.A., Triwitayakorn, K. and Tangphatsornruang, S. (2010). Construction of a genetic linkage map using simple sequence repeat markers from expressed sequence tags for cassava (Manihot esculenta Crantz). Molecular Breeding, doi:10.1007/s11032-010-9414-4.

99. Kuntaruk, S., Tatu, T., Keowkarnkah, T. and Kasinrerk, W. (2010). Sandwich ELISA for hemoglobin A

2 quantification

and identification of β-thalassemia carriers. International Journal of Hematology, 91(2), 219-228.

100. Kurdrid, P., Phuengcharoen, P., Cheevadhanarak, S., Tanticharoen, M. and Hongsthong, A. (2009). Identification of a heat shock-responsive cis-acting DNA sequence and its transcriptional regulator: Their roles in the expression of the Spirulina-desD gene in response to heat stress. Journal of Bioscience and Bioengineering, doi:10.1016/j.jbiosc.2009.09.002.

101. Lsse, T., Srikitikulchai, P., Fournier, J., Köpcke, B. and Stadler, M. (2010). Lepraric acid derivatives as chemotaxonomic markers in Hypoxylon aeruginosum, Chlorostroma subcubisporum and C. cyaninum, sp. nov.. Fungal Biology, 114(5-6), 481-489.

102. Laohaphatanaleart, K., Piyachomkwan, K., Sriroth, K., Santisopasri, V. and Bertoft, E. (2009). A study of the internal structure in cassava and rice amylopectin. Starch-Starke, 61(10), 557-569.

103. Laohaphatanalert, K., Piyachomkwan, K., Sriroth, K. and Bertoft, E. (2010). The fine structure of cassava starch amylopectin Part 1: Organization of clusters. International Journal of Biological Macromolecules, 47(3), 317-324.

104. Laoprom, N., Sithithaworn, P., Ando, K., Sithithaworn, J., Wongkham, S., Laha, T., Klinbunga, S., Webster, J.P. and Andrews, R.H. (2010). Microsatellite loci in the carcinogenic liver fluke, Opis horchis viverrini and their application as population genetic markers. Infection Genetics and Evolution, 10(1), 146-153.

105. Lee, V.S., Tue-ngeun, P., Nangola, S., Kitidee, K., Jitonnom, J., Nimmanpipug, P., Jiranusornkul, S. and Tayapiwatana, C. (2010). Pairwise decomposition of residue interaction energies of single chain Fv with HIV-1 p17 epitope variants. Molecular Immunology, 47(5), 982-990.

106. Limkhuansuwan, V. and Chaiprasert, P. (2010). Decolorization of molasses melanoidins and palm oil mill effluent phenolic compounds by fermentative lactic acid bacteria. Journal of Environmental Sciences-China, 22(8), 1209-1217.

107. Limtong, S., Kaewwichian, R., Am-In, S., Nakase, T., Lee, C.-F. and Yongmanitchai, W. (2010). Candida asiatica sp. nov., an anamorphic ascomycetous yeast species isolated from natural samples from Thailand, Taiwan,


and Japan. Antonie Van Leeuwenhoek International Journal of General and Molecular Microbiology, doi:10. 1007/s10482-010-9463-z.

108. Luangsa-ard, J.J., Ridkaew, R., Mongkolsamrit, S., Tasanathai, K. and Hywel-Jones, N.L. (2010). Ophiocordyceps barnesii and its relationship to other melolonthid pathogens with dark stromata. Fungal Biology, 114(9), 739-745.

109. Maczey, N., Dhendup, K., Cannon, P., Hywel-Jones, N. and Rai, T.B. (2010). Thitarodes namnai sp. nov. and T. caligophilus sp. nov. (Lepidoptera: Hepialidae), hosts of the economically important entomopathogenic fungus Ophiocordyceps sinensis in Bhutan. Zootaxa, 2412, 42-52.

110. Makarasara, W., Kumasaka, N., Assawamakin, A., Takahashi, A., Intarapanich, A., Ngamphiw, C., Kulawonganunchai, S., Ruangrit, U., Fucharoen, S., Kamatani, N. and Tongsima, S. (2009). pHCR: a Parallel Haplotype Configuration Reduction algorithm for haplotype interaction analysis. Journal of Human Genetics, 54(11), 634-641.

111. Malimas, T., Yukphan, P., Lundaa, T., Muramatsu, Y., Takahashi, M., Kaneyasu, M., Potacharoen, W., Tanasupawat, S., Nakagawa, Y., Suzuki, K., Tanticharoen, M. and Yamada, Y. (2009). Gluconobacter kanchanaburiensis sp. nov., a brown pigment-producing acetic acid bacterium for Thai isolates in the Alphaproteobacteria. Journal of General and Applied Microbiology, 55(3), 247-254.

112. Meunpol, O., Duangjai, E., Yoonpun, R. and Piyatiratitivorakul, S. (2010). Detection of prostaglandin E

2 in polychaete

Perinereis sp. and its effect on Penaeus monodon oocyte development in vitro. Fisheries Science, 76(2), 281-286.

113. Mongkolbovornkij, P., Champreda, V., Sutthisripok, W. and Laosiripojana, N. (2010). Esterification of industrial-grade palm fatty acid distillate over modified ZrO

2 (with WO

3–,

SO4 –and TiO

2–): Effects of co-solvent adding and

water removal. Fuel Processing Technology, 91(11), 1510-1516.

114. Moonsom, S., Tayapiwatana, C., Wongkham, S., Kongtawelert, P. and Kasinrerk, W. (2010). A Competitive ELISA for Quantifying Serum CD147: Reduction of Soluble CD147 Levels in Cancer Patient Sera. Hybridoma, 29(1), 45-52.

115. Nakase, T., Jindamorakot, S., Am-In, S., Ninomiya, S. and Kawasaki, H. (2010). Candida tanticharoeniae sp. nov., a novel anamorphic yeast species found in Thailand. Journal of General and Applied Microbiology, 56(1), 89-92.

116. Nakase, T., Jindamorakot, S., Tanaka, K., Ninomiya, S., Kawasaki, H., Limtong, S. and Lee, C.-F. (2010). Vanderwaltozyma tropicalis sp. nov., a novel ascomycetous yeast species found in Thailand. Journal of General and Applied Microbiology, 56(1), 31-36.

117. Nangola, S., Minard, P. and Tayapiwatana, C. (2010). Appraisal of translocation pathways for displaying ankyrin repeat protein on phage particles. Protein Expression and Purification, doi:10.1016/j.pep.2010.08.010.

118. Nasomphan, W., Tangboriboonrat, P. and Smanmoo, S. (2010). Selective Fluorescence Sensing of Deoxycytidine 5′-Monophosphate (dCMP) Employing a Bis (diphenylphosphate) diimine Ligand. Journal of Fluorescence, doi:10.1007/s10895-010-0703-4.

119. Netsawang, J., Noisakran, S., Puttikhunt, C., Kasinrerk, W., Wongwiwat, W., Malasit, P., Yenchitsomanus, P.T. and Limjindaporn, T. (2010). Nuclear localization of dengue virus capsid protein is required for DAXX interaction and apoptosis.Virus Research, 147(2), 275-283.

120. Nettuwakul, C., Sawasdee, N. and Yenchitsomanus, P. (2010). Rapid detection of solute carrier family 4, member 1 (SLC4A1) mutations and polymorphisms by high-resolution melting analysis. Clinical Biochemistry, 43(5), 497-504.

121. Nimitphak, T., Meemetta, W., Arunrut, N., Senapin, S. and Kiatpathomchai, W. (2010). Rapid and sensitive detection of Penaeus monodon nucleopolyhedrovirus (PemoNPV) by loop-mediated isothermal amplification combined with a lateral-flow dipstick. Molecular and Cellular Probes, 24(1), 1-5.

122. Nitiyon, S., Boonmak, C., Am-In, S., Jindamorakot, S., Kawasaki, H., Yongmanitchai, W. and Limtong, S. (2010). Candida saraburiensis sp. nov. and Candida prachuapensis sp. nov., two xylose-utilizing yeast species isolated in Thailand. International Journal of Systematic and Evolutionary Microbiology, doi:10.1099/ijs.0.023317-0.

123. Noisakran, S., Onlamoon, N., Songprakhon, P., Hsiao, H.M., Chokephaibulkit, K. and Perng, G.C. (2010). Cells in Dengue Virus Infection In Vivo. Advances in Virology, 2010(Article ID 164878).

124. Noophan, P., Paopuree, P., Kanlayaras, K., Sirivithayapakorn, S. and Techkarnjanaruk. S. (2009). Nitrogen Removal Efficiency at Centralized Domestic Wastewater Treatment Plants in Bangkok, Thailand. Environment Asia, 2(2), 30-35.

125. Nuclear, P., Sommit, D., Boonyuen, N. and Pudhom, K. (2010). Butenolide and furandione from an endophytic Aspergillus terreus. Chemical and Pharmaceutical Bulletin, 58(9), 1221-1223.

126. Nzila, A., Rottmann, M., Chitnumsub, P., Kiara, S.M., Kamchonwongpaisan, S., Maneeruttanarungroj, C., Taweechai, S., Yeung, B.K.S., Goh, A., Lakshminaraya-na, S.B., Zou, B., Wong, J., Ma, N.L., Weaver, M., Keller, T.H., Dartois, V., Wittlin, S., Brun, R., Yuthavong, Y. and Diagana, T.T. (2010). Preclinical Evaluation of the Antifolate QN254, 5-Chloro-N'6'-(2,5-Dimethoxy-Benzyl)-Quinazoline-2,4,6-Triamine, as an Antimalarial Drug Candidate. Antimicrobial Agents and Chemotherapy, 54(6), 2603-2610.

127. Onlamoon, N., Noisakran, S., Hsiao, H.M., Duncan, A., Villinger, F., Ansari, A.A. and Perng, G.C. (2010). Dengue virus-induced hemorrhage in a nonhuman primate model. Blood, 115(9), 1823-1824.

128. Paepatung, N., Nopharatana, A. and Songkasiri, W. (2009). Bio-methane potential of biological solid materials and agricultural wastes. Asian Journal of Energy and Environment, 10(1), 19-27.

129. Panichnumsin, P., Nopharatana, A., Ahring, B. and Chaiprasert, P. (2010). Production of methane by co-digestion of cassava pulp with various concentrations of pig manure. Biomass and Bioenergy, 34(8), 1117-1124.


130. Pengsuk, C., Longyant, S., Rukpratanporn, S., Chaivisuthangkura, P., Sridulyakul, P. and Sithigorngul, P. (2010). Development of monoclonal antibodies for simple detection and differentiation of Vibrio mimicus from V. cholerae and Vibrio spp. by dot blotting. Aquaculture, 300(1-4), 17-24.

131. Phannachet, K., Raksat, P., Limvuttegrijeerat, T. and Promdonkoy, B. (2010). Production and Characterization of N- and C-terminally Truncated Mtx2: a Mosquitocidal Toxin from Bacillus sphaericus. Current Microbiology, doi:10. 1007/s00284-010-9651-0.

132. Piccinato, C.A., Sartori, R., Sangsritavong, S., Souza, A.H., Grummer, R.R., Luchini, D. and Wiltbank, M.C. (2010). In vitro and in vivo analysis of fatty acid effects on metabolism of 17β-estradiol and progesterone in dairy cows. Journal of Dairy Science, 93(5), 1934-1943.

133. Pinruan, U., Rungjindamai, N., Choeyklin, R., Lumyong, S., Hyde, K.D. and Jones, E.B.G. (2010). Occurrence and diversity of basidiomycetous endophytes from the oil palm, Elaeis guineensis in Thailand. Fungal Diversity, 41(1), 71-88.

134. Pinruan, U., Rungjindamai, N., Sakayaroj, J., Lumyong, S., Hyde, K.D. and Jones, E.B.G. (2010). Baipadisphaeria gen. nov., a freshwater ascomycete (Hypocreales, Sordariomycetes) from decaying palm leaves in Thailand. Mycosphere, 1, 6.

135. Piriyapongsa, J., Ngamphiw, C., Assawamakin, A., Wangkumhang, P., Suwannasri, P., Ruangrit, U., Agavatpanitch, G. and Tongsima, S. (2009). RExPrimer: an integrated primer designing tool increases PCR effectiveness by avoiding 3' SNP-in-primer and mis- priming from structural variation. BMC Genomics, 10(Suppl 3), S4.

136. Pittayakhajonwut, P., Dramae, A., Intaraudom, C., Boonyuen, N., Nithithanasilp, S., Rachtawee, P. and Laksanacharoen, P. (2010). Two New Drimane Sesquiterpenes, Fudecadiones A and B, from the Soil Fungus Penicillium sp. BCC 17468. Planta Medica, PMID: 20577947.

137. Pongtippatee, P., Luppanakane, R., Thaweethamsewee, P., Kirirat, P., Weerachatyanukul, W. and Withyachumnarnkul, B. (2010). Delay of the egg activation process in the Black Tiger Shrimp Penaeus monodon by manipulation of magnesium levels in spawning water. Aquaculture Research, 41(2), 227-232.

138. Praipue, P., Klinbunga, S. and Jarayabhand, P. (2010). Genetic diversity of wild and domesticated stocks of Thai abalone, Haliotis asinina (Haliotidae), analyzed by single-strand conformational polymorphism of AFLP- derived markers. Genetics and Molecular Research, 9(2), 1136-1152.

139. Prasertlux, S., Khamnamtong, B., Chumtong, P., Klinbunga, S. and Menasveta, P. (2010). Expression levels of RuvBL2 during ovarian development and association between its single nucleotide polymorphism (SNP) and growth of the giant tiger shrimp Penaeus monodon. Aquaculture, 308(Suppl.1), S83-S90.

140. Preechaphol, R., Klinbunga, S., Ponza, P. and Menasveta,

P. (2010). Isolation and characterization of progesterone receptor-related protein p23 (Pm-p23) differentially expressed during ovarian development of the giant tiger shrimp Penaeus monodon. Aquaculture, 308(Suppl.1), S75-S82.

141. Preechaphol, R., Klinbunga, S., Yamano, K. and Menasveta, P. (2010). Molecular cloning and expression of progestin membrane receptor component 1 (Pgmrc1) of the giant tiger shrimp Penaeus monodon. General and Comparative Endocrinology, 168(3), 440-449.

142. Puthawibool, T., Senapin, S., Flegel, T.W. and Kiatpathomchai, W. (2010). Rapid and sensitive detection of Macrobrachium rosenbergii nodavirus in giant freshwater prawns by reverse transcription loop-mediated isothermal amplification combined with a lateral flow dipstick. Molecular and Cellular Probes, 24(5), 244-249.

143. Raita, M., Champreda, V. and Laosiripojana, N. (2010). Biocatalytic ethanolysis of palm oil for biodiesel production using microcrystalline lipase in tert-butanol system. Process Biochemistry, 45(6), 829-834.

144. Roongsawang, N., Promdonkoy, P., Wongwanichpokhin, M., Sornlake, W., Puseenam, A., Eurwilaichitr, L. and Tanapongpipat, S. (2010). Coexpression of fungal phytase and xylanase utilizing the cis-acting hydrolase element in Pichia pastoris. FEMS Yeast Research, doi:10.1111/j.1567-1364.2010.00669.x.

145. Rosa, C.A., Jindamorakot, S., Limtong, S., Nakase, T., Pagnocca, F.C., Lachance, M.-A. (2010). Candida golubevii sp. nov., an asexual yeast related to Metschnikowia lunata. International Journal of Systematic and Evolutionary Microbiology, 60(3), 704-706.

146. Ruenwai, R., Cheevadhanarak, S., Rachdawong, S., Tanticharoen, M. and Laoteng, K. (2010). Oxygen-induced expression of ∆6-, ∆9- and ∆12-desaturase genes modulates fatty acid composition in Mucor rouxii. Applied Microbiology and Biotechnology, 86(1), 327-334.

147. Rukachaisirikul, V., Arunpanichlert, J., Sukpondma, Y., Phongpaichit, S. and Sakayaroj, J. (2009). Metabolites from the endophytic fungi Botryosphaeria rhodina PSU-M35 and PSU-M114. Tetrahedron, 65(51), 10590-10595.

148. Rukachaisirikul, V., Khamthong, N., Sukpondma, Y., Pakawatchai, C., Phongpaichit, S., Sakayaroj, J. and Kirtikara, K. (2009). An [11]cytochalasin derivative from the marine-derived fungus Xylaria sp. PSU-F100. Chemical and Pharmaceutical Bulletin, 57(12), 1409-1411.

149. Rukachaisirikul, V., Khamthong, N., Sukpondma, Y., Phongpaichit, S., Hutadilok-Towatana, N., Graidist, P., Sakayaroj, J. and Kirtikara, K. (2010). Cyclohexene, diketopiperazine, lactone and phenol derivatives from the sea fan-derived fungi Nigrospora sp. PSU-F11 and PSU-F12. Archives of Pharmacal Research, 33(3), 375-380.

150. Rungrassamee, W., Leelatanawit, R., Jiravanichpaisal, P., Klinbunga, S. and Karoonuthaisiri, N. (2010). Expression and distribution of three heat shock protein genes under heat shock stress and under exposure to Vibrio harveyi in Penaeus monodon. Developmental and Comparative Immunology, 34(10), 1082-1089.

151. Sakayaroj, J., Preedanon, S., Supaphon, O., Jones, E.B.G.


and Phongpaichit, S. (2010). Phylogenetic diversity of endophyte assemblages associated with the tropical seagrass Enhalus acoroides in Thailand. Fungal Diversity, doi10.1007/s13225-009-0013-9.

152. Saksmerprome, V., Charoonnart, P., Gangnonngiw, W. and Withayachamnankul, B. (2009). A novel and inexpensive application of RNAi technology to protect shrimp from viral disease. Journal of Virological Methods, 162(1-2), 213-217.

153. Saksmerprome, V., Puiprom, O., Noonin, C. and Flegel, T.W. (2010). Detection of infectious hypodermal and haematopoietic necrosis virus (IHHNV) in farmed Australian Penaeus monodon by PCR analysis and DNA sequencing. Aquaculture, 298(3-4), 190-193.

154. Sangseethong, K., Termvejsayanon, N. and Sriroth, K. (2010). Characterization of physicochemical properties of hypochlorite- and peroxide-oxidized cassava starches. Carbohydrate Polymers, 82(2), 446-453.

155. Sangsuriya, P., Rojtinnakorn, J., Senapin, S. and Flegel, T.W. (2010). Identification and characterization of Alix/AIP1 interacting proteins from the black tiger shrimp Penaeus monodon. Journal of Fish Diseases, 33(7), 571-581.

156. Schoch, C.L., Crous, P.W., Groenewald, J.Z., Boehm, E.W.A., Burgess, T.I., de Gruyter, J., de Hoog, G.S., Dixon, L.J., Grube, M., Gueidan, C., Harada, Y., Hatakeyama, S., Hirayama, K., Hosoya, T., Huhndorf, S.M., Hyde, K.D., Jones, E.B.G., Kohlmeyer, J., Kruys, A., Li, Y.M., Lücking, R., Lumbsch, H.T., Marvanová, L., Mbatchou, J.S., MaVay, A.H., Miller, A.N., Mugambi, G.K., Muggia, L., Nelsen, M.P., Nelson, P., Owensby, C.A., Phillips, A.J.L., Phongpaichit, S., Pointing, S.B., Pujade-Renaud, V., Raja, H.A., Rivas Plata, E., Robbertse, B., Ruibal, C., Sakayaroj, J., Sano, T., Selbmann, L., Shearer, C.A., Shirouzu, T., Slippers, B., Suetrong, S., Tanaka, K., Volkmann-Kohlmeyer, B., Wingfield, M.J., Wood, A.R., Woudenberg, J.H.C., Yonezawa, H., Zhang, Y. and Spatafora, J.W. (2009). A class-wide phylogenetic assessment of Dothideomycetes. Studies in Mycology, 64(1), 1-15.

157. Senapin, S., Molthathong, S., Phiwsaiya, K., Jaengsanong, C. and Chuchird, N. (2010). Application of high resolution melt (HRM) analysis for duplex detection of Macrobrachium rosenbergii nodavirus (MrNV) and extra small virus (XSV) in shrimp. Molecular and Cellular Probes, 24(5), 291-297.

158. Senapin, S., Phiwsaiya, K., Anantasomboon, G., Sriphaijit, T., Browdy, C.L. and Flegel, T.W. (2010). Knocking down a Taura syndrome virus (TSV) binding protein Lamr is lethal for the whiteleg shrimp Penaeus vannamei. Fish and Shellfish Immunology, 29(3), 422-429.

159. Senapin, S., Thaowbut, Y., Gangnonngiw, W., Chuchird, N., Sriurairatana, S. and Flegel, T.W. (2010). Impact of yellow head virus outbreaks in the whiteleg shrimp, Penaeus vannamei (boone), in Thailand. Journal of Fish Diseases, 33(5), 421-430.

160. Shrestha, A., Dhamwichukorn, S. and Jenwitheesuk, E. (2010). Modeling of pyruvate decarboxylases from ethanol producing bacteria. Bioinformation, 4(8), 378-

384.161. Singboottra, P., Pata, S., Tayapiwatana, C. and Kasinrerk,

W. (2010). A simultaneously detection of phagocytosis and alteration of cell surface molecules by flow cytometry. Asian Pacific Journal of Allergy and Immunology, 28, 159-164.

162. Singkhamanan, K., Promdonkoy, B., Chaisri, U. and Boonserm, P. (2010). Identification of amino acids required for receptor binding and toxicity of the Bacillus sphaericus binary toxin. FEMS Microbiology Letters, 303(1), 84-91.

163. Sinsereekul, N., Wangkam, T., Thamchaipenet, A., Srikhirin, T., Eurwilaichitr, L. and Champreda, V. (2010). Recombinant expression of BTA hydrolase in Streptomyces rimosus and catalytic analysis on polyesters by surface plasmon resonance. Applied Microbiology and Biotechnology, 86(6), 1775-1784.

164. Sirilun, S., Chaiyasut, C., Kantachote, D. and Luxananil, P. (2010). Characterisation of non human origin probiotic Lactobacillus plantarum with cholesterol-lowering property. African Journal of Microbiology Research, 4(10), 994-1000.

165. Siringam, K., Juntawong, N., Cha-Um, S. and Kirdmanee, C. (2009). Relationships between sodium ion accumulation and physiological characteristics in rice (Oryza sativa L. spp. indica) seedlings grown under iso-osmotic salinity stress. Pakistan Journal of Botany, 41(4), 1837-1850.

166. Sittikankaew, K., Hiransuchalert, R., Yocawibun, P., Yamano, K. and Klinbunga, S. (2010). Identification, characterization and expression of adipose differentiation-related protein (ADRP) gene and protein in ovaries of the giant tiger shrimp Penaeus monodon. Aquaculture, 308(Suppl.1), S91-S99.

167. Sivichai, S. and Boonyuen, N. (2010). Jahnula morakotii sp. nov. and J. appendicutata from a peat swamp in Thailand. Mycotaxon, 112, 475-481.

168. Sreethawong, T., Niyamapa, T., Neramitsuk, H., Rangsunvigit, P., Leethochawalit, M. and Chavadej, S. (2010). Hydrogen production from glucose-containing wastewater using an anaerobic sequencing batch reactor: Effects of COD loading rate, nitrogen content, and organic acid composition. Chemical Engineering Journal, 160(1), 322-332.

169. Sreewongchai, T., Toojinda, T., Thanintorn, N., Kosawang, C., Vanavichit, A., Tharreau, D. and Sirithunya, P. (2010). Development of elite indica rice lines with wide spectrum of resistance to Thai blast isolates by pyramiding multiple resistance QTLs. Plant Breeding, 129(2), 176-180.

170. Sri-indrasutdhi, V., Boonyuen, N., Suetrong, S., Chuaseeharonnachai, C., Sivichai, S. and Jones, E.B.G. (2010). Wood-inhabiting freshwater fungi from Thailand: Ascothailandia grenadoidia gen. et sp. nov., Canalisporium grenadoidia sp. nov. with a key to Canalisporium species (Sordariomycetes, Ascomycota). Mycoscience, 51(6), 411-420.

171. Sriket, C., Benjakul, S. and Visessanguan, W. (2010). Post-mortem changes of muscle from fresh water prawn (Macrobrachium rosenbergii) as influenced by spawning stages. Lwt-Food Science and Technology, 43(4), 608-616.

172. Sriphairoj, K., Klinbu-nga, S., Kamonrat, W. and Na-Nakorn, U. (2010). Species identification of four


economically important Pangasiid catfishes and closely related species using SSCP markers. Aquaculture, 308(Suppl.1), S47-S50.

173. Sriroth, K., Piyachomkwan, K., Wanlapatit, S. and Nivitchanyong, S. (2010). The promise of a technology revolution in cassava bioethanol: From Thai practice to the world practice. Fuel, doi:10.1016/j.fuel.2009.12.008.

174. Srisuk, C., Chaivisuthangkura, P., Sukhumsirichart, W., Sridulyakul, P., Longyant, S., Rukpratanporn, S. and Sithigorngul, P. (2010). Improved immunodetection of Penaeus monodon densovirus with monoclonal antibodies raised against recombinant capsid protein. Aquaculture, doi:10.1016/j.aquaculture.2010.08.018.

175. Srisuk, C., Chaivisuthangkura, P., Rukpratanporn, S., Longyant, S., Sridulyakul, P. and Sithigorngul, P. (2010). Rapid and sensitive detection of Vibrio cholerae by loop-mediated isothermal amplification targeted to the gene of outer membrane protein ompW. Letters in Applied Microbiology, 50(1), 36-42.

176. Sritunyalucksana, K., Srisala, J., Wangnai, W. and Flegel, T.W. (2010). Yellow head virus (YHV) transmission risk from commodity shrimp is reduced to negligible levels by normal processing. Aquaculture, 300(1-4), 32-36.

177. Sudhadham, M., Gerrits van den Ende, A.H.G., Sihanonth, P., Sivichai, S., Chaiyarat, R., Menken, S.B.J., van Belkum, A. and de Hoog, G.S. (2010). Elucidation of distribution patterns and possible infection routes of the neurotropic black yeast Exophiala dermatitidis using AFLP. Mycological Research, doi:10.1016/j.funbio.2010.07.004.

178. Suetrong, S., Schoch, C.L., Spatafora, J.W., Kohlmeyer, J., Volkmann-Kohlmeyer, B., Sakayaroj, J., Phongpaichit, S., Tanaka, K., Hirayama, K. and Jones, E.B.G. (2009). Molecular systematics of the marine Dothideomycetes. Studies in Mycology, 64(1), 155-173.

179. Sungsuwan, S., Ruangsupapichart, N., Prabpai, S., Kongsaeree, P. and Thongpanchang, T. (2010). Tetrahydro-1, 4-epoxynaphthalene-1-carboxylic acid: a chiral derivatizing agent for the determination of the absolute configuration of secondary alcohols. Tetrahedron Letters, 51(38), 4965-4967.

180. Supungul, P., Rimphanitchayakit, V., Aoki, T., Hirono, I. and Tassanakajon, A. (2010). Molecular characterization and expression analysis of a c-type and two novel muramidase-deficient i-type lysozymes from Penaeus monodon. Fish and Shellfish Immunology, 28(3), 490-498.

181. Suriyachadkun, C., Chunhametha, S., Thawai, C., Tamura, T., Potacharoen, W., Kirtikara, K., Sanglier, J.-J. and Kitpreechavanich, V. (2010). Planotetraspora kaengkrachanensis sp. nov. and Planote traspora phitsanulokensis sp. nov., isolated from soil. International Journal of Systematic and Evolutionary Microbiology, 60(9), 2076-2081.

182. Tangphatsornruang, S., Birch-Machin, I., Newell, C.A. and Gray, J.C. (2010). The effect of different 3′ untranslated regions on the accumulation and stability of transcripts of a gfp transgene in chloroplasts of transplastomic tobacco. Plant Molecular Biology, doi:10.1007/s11103-010-9689-1.

183. Tangphatsornruang, S., Sangsrakru, D., Chanprasert, J., Uthaipaisanwong, P., Yoocha, T., Jomchai, N. and Tragoonrung, S. (2010). The Chloroplast Genome Sequence of Mungbean (Vigna radiata) Determined by High-throughput Pyrosequencing: Structural Organization and Phylogenetic Relationships. Dna Research, 17(1), 11-22.

184. Tangphatsornruang, S., Somta, P., Uthaipaisanwong, P., Chanprasert, J., Sangsrakru, D., Seehalak, W., Sommanas, W., Tragoonrung, S. and Srinives, P. (2009). Characterization of microsatellites and gene contents from genome shotgun sequences of mungbean (Vigna radiata (L.) Wilczek). BMC Plant Biology, 9, 137.

185. Tantivitayakul, P., Panapruksachat, S., Billamas, P. and Palittapongarnpim, P. (2010). Variable number of tandem repeat sequences act as regulatory elements in Mycobacterium tuberculosis. Tuberculosis, 90(5), 311-318.

186. Tapingkae, W., Parkin, K.L., Tanasupawat, S., Kruenate, J., Benjakul, S. and Visessanguan, W. (2010). Whole cell immobilisation of Natrinema gari BCC 24369 for histamine degradation. Food Chemistry, 120(3), 842-849.

187. Tapingkae, W., Tanasupawat, S., Parkin, K.L., Benjakul, S. and Visessanguan, W. (2010). Degradation of histamine by extremely halophilic archaea isolated from high salt-fermented fishery products. Enzyme and Microbial Technology, 46(2), 92-99.

188. Tassanakajon, A., Amparyup, P., Somboonwiwat, K. and Supungul, P. (2010). Cationic Antimicrobial Peptides in Penaeid Shrimp. Marine Biotechnology, 12(5), 487-505.

189. Techaprasan, J., Klinbunga, S., Ngamriabsakul, C. and Jenjittikul, T. (2010). Genetic variation of Kaempferia (Zingiberaceae) in Thailand based on chloroplast DNA (psbA-trnH and petA-psbJ) sequences. Genetics and Molecular Research, 9(4), 1957-1973.

190. Thammachat, S., Pungtanom, N., Kidsanguan, S., Pathaichindachote, W., Promdonkoy, B. and Krittanai, C. (2010). Amino acid substitution on β1 and αF of Cyt2Aa2 affects molecular interaction of protoxin. BMB Reports, 43(6), 427-431.

191. Thammasorn, W., Eadjongdee, K., Hongsthong, A., Porkaew, K. and Cheevadhanarak, S. (2009). Probability-Based Scoring Function as a Software Tool Used in the Genome-Based Identification of Proteins from Spirulina platensis. The Open Bioinformatics Journal, 3, 59-68.

192. Theeraapisakkun, M., Klinbunga, S. and Sittipraneed, S. (2010). Development of a species-diagnostic marker and its application for population genetics studies of the stingless bee Trigona collina in Thailand. Genetics and Molecular Research, 9(2), 919-930.

193. Thong-On, A., Smittipat, N., Juthayothin, T., Yanai, H., Yamada, N., Yorsangsukkamol, J., Chaiprasert, A., Rienthong, D., Billamas, P. and Palittapongarnpim, P. (2010). Variable-number tandem repeats typing of Mycobacterium tuberculosis isolates with low copy numbers of IS6110 in Thailand. Tuberculosis, 90(1),9-15.

194. Thummajitsakul, S., Klinbunga, S. and Sittipraneed, S. (2010). Development of a Species-Diagnostic Marker for Identification of the Stingless Bee Trigona pagdeni in Thailand. Biochemical Genetics, 48(3-4), 181-192.


195. Thuzar, M., Vanavichit, A., Tragoonrung, S. and Jantasuriyarat, C. (2010). Efficient and rapid plant regeneration of oil palm zygotic embryos cv. ‘Tenera’ through somatic embryogenesis. Acta Physiologiae Plantarum, doi:10.1007/s11738-010-0526-6.

196. Touch, V., Keo, S., Songkasiri, W., Stefan, C., Commins, T., Khouangvichit, S. and Green, J. (2009). Use of solid waste for small-scale biogas production for rural household energy consumption case study in takaev province, cambodia. Asian Journal of Energy and Environment, 10(1), 1-10.

197. Trisuwan, K., Rukachaisirikul, V., Sukpondma, Y., Phongpaichit, S., Preedanon, S. and Sakayaroj, J. (2010). Furo[3,2-h]isochroman, furo[3,2-h]isoquinoline, isochroman, phenol, pyranone, and pyrone derivatives from the sea fan-derived fungus Penicillium sp. PSU-F40. Tetrahedron, 66(25), 4484-4489.

198. Ungsupravate, D., Sawasdee, N., Khositseth, S., Udomchaiprasertkul, W., Khoprasert, S., Li, J., Reithmeier, R.A.F. and Yenchitsomanus, P.-T. (2010). Impaired trafficking and intracellular retention of mutant kidney anion exchanger 1 proteins (G701D and A858D) associated with distal renal tubular acidosis. Molecular Membrane Biology, 27(2-3), 92-103.

199. Visudtiphole, V., Watthanasurorot, A., Klinbunga, S., Menasveta, P. and Kirtikara, K. (2010). Molecular characterization of Calreticulin: A biomarker for temperature stress responses of the giant tiger shrimp Penaeus monodon. Aquaculture, 308(Suppl.1), S100-S108.

200. Vrydaghs, L., Ball, T., Volkaert, H., Houwe, I.V., Manwaring, J. and Langhe, E.D. (2009). Differentiating the Volcaniform Phytoliths of Bananas: Musa acuminata. Ethnobotany Research and Applications, 7, 239-246.

201. Wadbua, P., Promdonkoy, B., Maensiri, S. and Siri, S. (2010). Different properties of electrospun fibrous scaffolds of separated heavy-chain and light-chain fibroins of Bombyx mori. International Journal of Biological Macromolecules, 46(5), 493-501.

202. Wang, H.W., Noland, C., Siridechadilok, B., Taylor, D.W., Ma, E., Felderer, K., Doudna, J.A. and Nogales, E. (2009). Structural insights into RNA processing by the human RISC-loading complex. Nature Structural and Molecular Biology, 16(11), 1148-1153.

203. Wangchuk, P., Bremner, J.B., Samten, Rattanajak, R. and Kamchonwongpaisan, S. (2009). Antiplasmodial agents from the Bhutanese medicinal plant Corydalis calliantha. Phytotherapy Research, doi 10.1002/ptr.2893.

204. Wangchuk, P., Bremner, J.B., Samten, Skelton, B.W., White, A.H., Rattanajak, R. and Kamchonwongpaisan, S. (2010). Antiplasmodial activity of atisinium chloride from the Bhutanese medicinal plant, Aconitum orochryseum. Journal of Ethnopharmacology, 130(3), 559-562.

205. Wanichananan, P., Teerakathiti, T., Roytrakul, S., Kirdmanee, C. and Peyachoknagul, S. (2010). A highly efficient method for Agrobacterium mediated transformation in elite rice varieties (Oryza sativa L. spp. indica). African Journal of Biotechnology, 9(34), 5488-5495.

206. Wanitchang, A., Kramyu, J. and Jongkaewwattana, A. (2010). Enhancement of Reverse Genetics-derived Swine-Origin

H1N1 Influenza Virus Seed Vaccine Growth by Inclusion of Indigenous Polymerase PB1 Protein. Virus Research, 147(1), 145-148.

207. Washio, K., Lim, S.P., Roongsawang, N. and Morikawa, M. (2010). Identification and Characterization of the Genes Responsible for the Production of the Cyclic Lipopeptide Arthrofactin by Pseudomonas sp. MIS38. Bioscience Biotechnology and Biochemistry, 74(5), 992-999.

208. Watthanasurorot, A., Jiravanichpaisal, P., Sderhll, I. and Sderhll, K. (2010). A gC1qR prevents WSSV replication in the freshwater crayfish Pacifastacus leniusculus. Journal of Virology, doi:10.1128/JVI.01045-10.

209. Wittayacom, K., Uthaipibull, C., Kumpornsin, K., Tinikul, R., Kochakarn, T., Songprakhon, P. and Chookajorn, T. (2010). A nuclear targeting system in Plasmodium falciparum. Malaria Journal, 9, 126.

210. Wongsaprom, C., Sirithunya, P., Vanavichit, A., Pantuwan, G., Jongdee, B., Sidhiwong, N., Lanceras-Siangliw, J. and Toojinda, T. (2010). Two introgressed quantitative trait loci confer a broad-spectrum resistance to blast disease in the genetic background of the cultivar RD6 a Thai glutinous jasmine rice. Field Crops Research, 119(2-3), 245-251.

211. Wongsurawat, T., Leelatanawit, R., Thamniemdee, N., Uawisetwathana, U., Karoonuthaisiri, N., Menasveta, P. and Klinbunga, S. (2010). Identification of testis-relevant genes using in silico analysis from testis ESTs and cDNA microarray in the black tiger shrimp (Penaeus monodon). BMC Molecular Biology, 11, 55.

212. Wongwilaiwalin, S., Rattanachomsri, U., Laothanachareon, T., Eurwilaichitr, L., Igarashi, Y. and Champreda, V. (2010). Analysis of a thermophilic lignocellulose degrading microbial consortium and multi-species lignocellulolytic enzyme system. Enzyme and Microbial Technology, 47(6), 283-290.

213. Xu, S., Kangwanpong, D., Seielstad, M., Srikummool, M., Kampuansai, J., Jin, L. and The HUGO Pan-Asian SNP Consortium. (2010). Genetic evidence supports linguistic affinity of Mlabri - a hunter-gatherer group in Thailand. BMC Genetics, 11(1), 18.

214. Yukphan, P., Malimas, T., Lundaa, T., Muramatsu, Y., Takahashi, M., Kaneyasu, M., Tanasupawat, S., Nakagawa, Y.,Suzuki, K.-I.,Tanticharoen, M. and Yamada, Y. (2010). Gluconobacter wancherniae, sp. nov., an acetic acid bacterium from Thai isolates in the α-Proteobacteria. Journal of General and Applied Microbiology, 56(1), 67-73.

215. Yukphan, P., Malimas, T., Muramatsu, Y., Takahashi, M., Kaneyasu, M., Potacharoen, W., Tanasupawat, S., Nakagawa, Y., Hamana, K., Tahara, Y., Suzuki, K., Tanticharoen, M. and, Yamada, Y. (2009). Ameyamaea chiangmaiensis gen. nov., sp. nov., an Acetic Acid Bacterium in the α-Proteobacteria. Bioscience Biotechnology and Biochem-istry, 73(10), 2156-2162.

216. Zhang, Y., Sderhll, I., Sderhll, K. and Jiravanichpaisal, P. (2010). Expression of immune-related genes in one phase of embryonic development of freshwater crayfish, Pacifastacus leniusculus. Fish and Shellfish Immunology, 28(4), 649-653.


LIST OF PATENTS AND PETTY PATENTS

List of Patent / Petty Patent Applications

Title Filing Date Ref. No.

Xanthone analogues with anti-herpes simplex activity 16 October 2009 0903001233(Petty patent)

Xanthones with anti-herpes simplex activity 16 October 2009 0901004650

Calculation of marbling fat for meat quality determination by using random image processing technique

13 November 2009 0901005076

Biological process for degradation of histamine in high salt containing foods by using halophilic archaea producing histamine dehydrogenase

20 November 2009 0901005189

Method for production of monoclonal antibodies having desired isotypes 18 December 2009 0901005713

The use of yeast cell with phytase on cell surface in combination with a polysaccharide-degrading enzyme to improve nutrient in animal feed

15 January 2010 1003000039(Petty patent)

DNA vector for expressing gene responsible for the production of protein and metabolite in filamentous fungi

22 January 2010 1003000062(Petty patent)

Immunoassay methods for detecting bacterial fruit blotch in cucurbits using monoclonal antibody specific to Acidovorax avenae subsp. citrulli

19 February 2010 1001000265

Hemoglobin E (HbE) tube kit 26 February 2010 1001000304

Recombinant plasmid for the co-production and secretion of at least 2 enzymes and production thereof

25 March 2010 1003000265(Petty patent)

Pyrone compound that can inhibit the growth of cancer cell 1 April 2010 1001000566

Procedure for peptide inhibitor design 29 April 2010 1001000686

A gene expression-based method for serological identification of autoantibodies to cancer cells

29 April 2010 1003000366(Petty patent)

Continuous biogas production unit 27 May 2010 1003000451(Petty patent)

PCR detection method for infectious hypodermal and haematopoietic necrosis virus (IHHNV) in black tiger shrimp using primer pairs to cover the whole IHHNV genome

10 June 2010 1001000856

Determination of bovine sperm quality by measuring the RNA level of protamine gene

24 June 2010 1003000546(Petty patent)

Detection of porcine growth rate associated SNPs by using allele specific PCR


Detection of porcine production performance and litter size associated SNPs by using allele-specific PCR


Detection of porcine meat tenderness and meat quality associated SNPs by using allele-specific PCR


Detection of porcine growth rate associated SNPs 24 June 2010 1003000550(Petty patent)

Detection of porcine production performance and litter size associated SNPs 24 June 2010 1003000551(Petty patent)

Detection of porcine meat tenderness and meat quality associated SNPs 24 June 2010 1003000552(Petty patent)

Unless otherwise specified, all titles presented in the table are for patents filed in Thailand.


Title Filing Date Ref. No.

Detection of gene defect associated with canine drug allergy 24 June 2010 1003000553(Petty patent)

Detection of bovine marbling fat and meat tenderness associated SNPs by using loop-mediated isothermal DNA amplification


Method for lactic acid bacteria identification using DNA microarray 28 July 2010 1001001146

Sucrose measuring device based on pulse amperometry for sugar mill factory

2 August 2010 1003000692(Petty patent)

Simultaneously serotyping of dengue virus by using anti-dengue NS1 monoclonal antibodies

11 August 2010 1003000726(Petty patent)

Methods to enhance expression of influenza A virus neuraminidase in mammalian cells using influenza non-structural protein 1

20 August 2010 1001001267

A rapid dual-labelling bioassay for screening of anti-anthracnose agents 2 September 2010 1003000806(Petty patent)

Construction of a red fluorescent Colletotrichum capsici recombinant strain and its application

2 September 2010 1003000807(Petty patent)

Sandworm harvesting device 16 September 2010 1003000884(Petty patent)

Protocol for clonal propagation of oil palm plantlets (Elaeis guineensis Jacq.) 27 September 2010 1001001479

An effective protocol for rice (Oryza sativa L.) flowering and seed set in plant tissue culture system

30 September 2010 1001001519

DNA plasmid for the production of Vegetative insecticidal proteins (Vips) in Bacillus spp. hosts and its application.

30 September 2010 1001001520

List of Granted Patents / Petty Patents

Title Granting Date (Petty) Patent No.

Determination of risk of developing dengue hemorrhagic fever/dengue shock syndrome, methods and compositions therefor

8 December 2009 US7629117(USA)

TB detection kit using one-tube nested PCR technique 8 January 2010 TH27265

Nucleic acids that enhance the synthesis of 2-acetyl-1-pyrroline in plants and fungi

12 January 2010 VN8180(Vietnam)

Pollen trapping device 22 April 2010 TH5341(Petty patent)

Technique for long-term preservation of microbial cells 29 April 2010 TH16673

Dengue virus mutant strain, MBU 01-2002 25 May 2010 US7722885(USA)

Dried blood spot paper hole punching device for DNA extraction 25 May 2010 TH5396(Petty patent)

DNA detection of M. marinum and M. fortuitum complex 1 July 2010 TH28129

Identification of Mycobacterium tuberculosis and nontuberculous mycobacteria by one tube multiplex PCR

1 July 2010 TH28130

Unless otherwise specified, all titles presented in the table are for patents filed in Thailand.


Prof. Morakot TanticharoenSenior Advisor to the Executive DirectorAPBioChEC Award, presented at the Asia Pacific Biochemical Engineering Conference 2009 (APBioChEC’09) in Japan

Prof. Watchara KasinrerkBiomedical Technology Research CenterOutstanding Person of the Nation 2009 (Science and Technology), awarded by the National Identity Board, the Prime Minister’s

Office

Assoc. Prof. Dr. Apichart VanavichitRice Gene Discovery UnitFirst runner-up Innovation Award 2009, awarded by Kasetsart University

Dr. Verawat ChampredaBioresources Technology UnitYoung Scientist Award 2010 for his work on “Enzyme discovery from microbial resources in Thailand and enzyme application in

key local industries”, awarded by the Foundation for the Promotion of Science and Technology under the Patronage of His Majesty the King

Dr. Wonnop VisessanguanBIOTEC Central Research Unit NSTDA Inventor’s Day & Technopreneur Prize 2010 for his work on “Production of pentosanase enzyme from Aspergillus sp.

BCC7178 for animal feed”, awarded by the National Science and Technology Development Agency (NSTDA)

Dr. Chalermpol KirdmaneeBIOTEC Central Research Unit NSTDA Inventor’s Day & Technopreneur Prize 2010 for his work on “Air filter for air and moisture exchange for tissue culture

containers”, awarded by the National Science and Technology Development Agency (NSTDA)

Dr. Ekachai JenwitheesukGenome InstituteSecond runner-up in The NSTDA Idea to Product Competition 2009: Fast Track to Commercialization for his work on “Canine drug

allergy test kit”, awarded by Technology Management Center and Thammasart University

Dr. Nitsara Karoonuthaisiri et. al.(Dr. Oraprapai Gajanandana, Dr. Plearnpis Luxananil, Dr. Kanyawim Kirtikara, Mr. Ratthaphol Charlermroj and Ms. Umaporn

Uawisetwathana) BIOTEC Central Research Unit NRCT Invention Award 2010 for the development of “Antibody array for multiple detection of food-borne pathogens”, awarded by

the National Research Council of Thailand (NRCT)

Dr. Philip ShawBIOTEC Central Research UnitGrand Challenges Explorations Grant 2009 for a research project titled “A new tool for anti-malarial target gene validation”, awarded

by the Bill & Melinda Gates Foundation The Great Gigabase for a research project titled “Comprehensive Transcript Mapping of the 5’ ends of P. falciparum mRNA”,

awarded by Roche Diagnostics (Thailand) Ltd., Biogenomed Co., Ltd. and Genome Institute.

Dr. Nitsara Karoonuthaisiri BIOTEC Central Research UnitL’Oreal Thailand For Women in Science Fellowship 2009 (Life Science) for her work on “Application of Microarray Technology for

Research and Development in Thailand”, awarded by L’Oreal Thailand and the Thai National Commission for UNESCO

HONORS AND AWARDS


Dr. Chawanee ThongpanchangBioresources Technology Unit CST – Wiley Outstanding Publication Award 2009 for her work on “Immobilization of malarial (Plasmodium falciparum) dihydrofolate

reductase for the selection of tight-binding inhibitors from combinatorial library”, awarded by the Chemistry Society of Thailand

Dr. Nipa ChokesajjawateeBIOTEC Central Research Unit Endeavour Executive Awards 2010 for her work on “Principle and advanced application of predictive modeling for construction of

quantitative risk assessment model of Salmonella sp. in chicken”, awarded by the Australian Government

Dr. Alongkorn Amnuaykanjanasin Bioresources Technology Unit 2010 IFS Grant for a research project titled “Determination of potential role of reducing clade III PKS gene in the

entomopathogenic fungus Beauveria bassiana on pathogenesis against insect pests”, awarded by the International Foundation for Science (IFS)

Dr. Virak Visudtiphole BIOTEC Central Research Unit2010 IFS Grant for a research project titled “Characterization of genes and proteins related to Ca2+ homeostasis and stress in the

black tiger shrimp (Penaeus monodon)”, awarded by the International Foundation for Science (IFS)

Dr. Vanvimon Saksmerprome Center of Excellence for Shrimp Molecular Biology and Biotechnology 2010 IFS Grant for a research project titled “Production and delivery of double-stranded RNA to control yellow-head virus disease

in shrimp”, awarded by the International Foundation for Science (IFS) Best Oral Presentation 2009 for her work on “A novel and inexpensive application of RNAi technology to protect shrimp from viral

disease”, presented at the Meeting of Young Scientists and TRF Senior Research Scholars, organized by Thailand Research Fund (TRF)

Dr. Sithichoke Tangphatsornruang, et al. (Ms. Duangjai Sangsrakru, Ms. Juntima Chanprasert, Ms. Thippawan Yoocha, Ms. Pichahpuk Uthaipaisanwong, Ms. Nukoon Jomchai

and Dr. Somvong Tragoonrung) Genome Institute Paper titled “The chloroplast genome sequence of mungbean (Vigna radiata) determined by high-throughput pyrosequencing:

structural organization and phylogenetic relationships” was highlighted on A-IMBN Research, selected by the Asia-Pacific International Molecular Biology Network (A-IMBN)

Dr. Piyanun Harnpicharnchai, et. al.(Ms. Warasirin Sornlake, Mr. Kittapong Sae-Tang, Dr. Lily Eurwilaichitr and Dr. Sutipa Tanapongpipat)Bioresources Technology UnitPaper titled “Cell-surface phytase on Pichia pastoris cell wall offers great potential as a feed supplement” was highlighted on

A-IMBN Research, selected by the Asia-Pacific International Molecular Biology Network (A-IMBN)

Mr. Ukrit Rattanachomsri, et. al. (Dr. Sutipa Tanapongpipat, Dr. Lily Eurwilaichitr and Dr. Verawat Champreda) Bioresources Technology UnitJBB Excellent Paper Award for Overseas Researchers 2010 for the paper titled “Simultaneous non-thermal saccharification of cassava

pulp by multi-enzyme activity and ethanol fermentation by Candida tropicalis”, awarded by The Society for Biotechnology, Japan

Mr. Asawin Wanitchang and Dr. Anan Jongkaewwattana, et. al.(Research team of Virology and Cell Technology Laboratory)BIOTEC Central Research Unit Outstanding Poster Presentation Award on “Compatibility of strain-specific amino acids of PA of pandemic H1N1 influenza A virus

influences its polymerase function and virus growth”, presented at Thailand Conference on Emerging Infectious and Neglected Diseases (EID2010)


Ms. Pinpunya RiangrungrojBIOTEC Central Research Unit Best Poster Award 2010 (Third Prize) on “Biochemical characterization of adenosine deaminase from Plasmodium falciparum”,

presented at The 5th Annual Symposium of Protein Society of Thailand

Dr. Jittima PiriyapongsaGenome InstituteThesis Award 2009 (Agricultural Science and Biology) for the PhD thesis titled “Origin and evolution of eukaryotic gene sequences

derived from transposable elements”, awarded by the National Research Council of Thailand (NRCT)

Dr. Sumarin SoonsangaBioresources Technology Unit Thesis Award 2009 (Agricultural Science and Biology) for the PhD thesis titled “Sensing of organic hydroperoxide by Bacillus subtilis

OhrR”, awarded by the National Research Council of Thailand (NRCT)

Dr. Nanchaya WanasenBIOTEC Central Research Unit Thesis Award 2009 (Medical Science) for the PhD thesis titled “Immuopathogenic mechanisms of non – healing – cutaneous

leishmaniasis caused by Leishmania amazonensis”, awarded by the National Research Council of Thailand (NRCT)

Mr. Soonthorn Todam and Bala Conservation Youth GroupPeat Swamp and Hala-Bala Rain Forest Research Unit and Nikompattana 10 School, Narathiwat ProvinceGreen Globe Award 2009 (Youth Category) for the work on “Conservation and Integration of Biodiversity in Saiburi River into

School Curriculum”, awarded by PTT Public Company Limited


Advisors

Prof. Naksitte Coovattanachai Director of the Royal Golden Jubilee PhD Program, Thailand Research Fund (TRF)

Prof. Sanit Aksornkoae President, Thailand Environment Institute (TEI)

Dr. Sermpol Ratasuk Expert, TEAM Consulting Engineering and Management Co., Ltd. (TEAM)

Dr. Sakarindr Bhumiratana President, King Mongkut’s University of Technology Thonburi

Chairman

Prof. Sujin Jinahyon President, Naresuan University

Vice Chairman

Dr. Thaweesak Koanantakool President, National Science and Technology Development Agency (NSTDA)

Members

Prof. Narongsak Chaiyabutr Faculty of Veterinary Science, Chulalongkorn University

Prof. Prapon Wilairat Faculty of Science, Mahidol University

Mr. Pornsil Patchrintanakul Vice President, Charoen Pokphand Group Deputy Secretary General, Thai Chamber of Commerce

Prof. Peerasak Srinives Faculty of Agriculture, Kasetsart University

Dr. Vichai Chokevivat Director, Institute for Development of Human Research Protection

Mrs. Vipajaree Putthamilinprateep Bureau of the Budget

Prof. Sawasd Tantaratana Director, Thailand Research Fund (TRF)

Mr. Somchai Charnnarongkul Director General, Department of Agriculture

Dr. Somsak Chunharas Secretary General, National Health Foundation

Prof. Amaret Bhumiratana Faculty of Science, Mahidol University

Dr. Kanyawim Kirtikara Executive Director, BIOTEC

Ms. Dussadee Siamhan Deputy Executive Director, BIOTEC

International Advisory Board

Chairman

Prof. Ken-ichi Arai Professor Emeritus, The University of Tokyo, JAPAN

Members

Dr. Jill Conley Director, International and Precollege Science Education Programs Howard Hughes Medical Institute (HHMI), USA

Prof. Mauro Giacca Director, International Center for Genetic Engineering and Biotechnology (ICGEB) Trieste, ITALY

Prof. Paul Greenfield Vice-Chancellor, The University of Queensland, AUSTRALIA

Dr. Ming-Chu Hsu Chairman & CEO, TaiGen Biotechnology Co., Ltd., TAIWAN

Prof. Lene Lange Vice Dean for Faculty of Engineering, Science and Medicine, Aalborg University, DENMARK

Prof. Anthony Turner Commercial Director & Distinguished Professor of Biotechnology, Cranfield Health, Cranfield University, UK

Prof. Dyann Wirth Chair, Department of Immunology and Infectious Diseases, Harvard School of Public Health, USA

Prof. Albert Cheung Hoi Yu Vice-Director and Professor, Neuroscience Research Institute & Department of Neurobiology, Peking University, CHINA

Executives and Management Team

Executive Board

As of 30 September 2010


Management Team

Dr. Kanyawim KirtikaraExecutive Director

Ms. Dussadee SiamhanDeputy Executive Director

Dr. Suvit TiaDeputy Executive Director

Ms. Kruawan PotisombatAssistant Executive Director

Dr. Omjai SaimekAssistant Executive Director

As of 30 September 2010

Documents

Annual Report 2010 - biotec.or.th...Annual report 2010 National Center for Genetic Engineering and Biotechnology (BIOTEC) / National Center for Genetic Engineering and Biotechnology